ATE408824T1 - Miniaturisierte zellenanordnung und verfahren und vorrichtung zum screening mittels zellen - Google Patents

Miniaturisierte zellenanordnung und verfahren und vorrichtung zum screening mittels zellen

Info

Publication number
ATE408824T1
ATE408824T1 AT97928806T AT97928806T ATE408824T1 AT E408824 T1 ATE408824 T1 AT E408824T1 AT 97928806 T AT97928806 T AT 97928806T AT 97928806 T AT97928806 T AT 97928806T AT E408824 T1 ATE408824 T1 AT E408824T1
Authority
AT
Austria
Prior art keywords
cells
screening
methods
present
combining
Prior art date
Application number
AT97928806T
Other languages
English (en)
Inventor
D Taylor
Original Assignee
Cellomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc filed Critical Cellomics Inc
Application granted granted Critical
Publication of ATE408824T1 publication Critical patent/ATE408824T1/de

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
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    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT97928806T 1996-05-30 1997-05-29 Miniaturisierte zellenanordnung und verfahren und vorrichtung zum screening mittels zellen ATE408824T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1869696P 1996-05-30 1996-05-30

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ATE408824T1 true ATE408824T1 (de) 2008-10-15

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AT97928806T ATE408824T1 (de) 1996-05-30 1997-05-29 Miniaturisierte zellenanordnung und verfahren und vorrichtung zum screening mittels zellen

Country Status (7)

Country Link
EP (1) EP0912892B1 (de)
JP (1) JP2000512009A (de)
AT (1) ATE408824T1 (de)
AU (1) AU734704B2 (de)
CA (1) CA2257287A1 (de)
DE (1) DE69738996D1 (de)
WO (1) WO1997045730A1 (de)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6794127B1 (en) 1997-06-16 2004-09-21 Diversa Corporation Capillary array-based sample screening
US6972183B1 (en) 1997-06-16 2005-12-06 Diversa Corporation Capillary array-based enzyme screening
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
CN101419170A (zh) * 1996-08-16 2009-04-29 Ge保健尼亚加拉公司 用于分析井板、凝胶和斑点的数字成像系统
JP4663824B2 (ja) * 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド 多重化分子分析装置および方法
US6756207B1 (en) 1997-02-27 2004-06-29 Cellomics, Inc. System for cell-based screening
US6759206B1 (en) 1997-02-27 2004-07-06 Cellomics, Inc. System for cell-based screening
US6727071B1 (en) 1997-02-27 2004-04-27 Cellomics, Inc. System for cell-based screening
US7117098B1 (en) 1997-02-27 2006-10-03 Cellomics, Inc. Machine-readable storage medium for analyzing distribution of macromolecules between the cell membrane and the cell cytoplasm
US6416959B1 (en) 1997-02-27 2002-07-09 Kenneth Giuliano System for cell-based screening
US7853411B2 (en) 1997-02-27 2010-12-14 Cellomics, Inc. System for cell-based screening
US6548263B1 (en) 1997-05-29 2003-04-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US7160687B1 (en) 1997-05-29 2007-01-09 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
DE19742246A1 (de) * 1997-09-25 1999-04-01 Basf Ag Analytisches Meßverfahren und seine Verwendung
WO1999039184A1 (en) * 1998-01-29 1999-08-05 Ribozyme Pharmaceuticals, Inc. Image acquisition and image analysis of biological material
WO1999052625A2 (de) * 1998-04-09 1999-10-21 Institut für Diagnostikforschung GmbH an der Freien Universität Berlin Verfahren und vorrichtung zur anfertigung von synthese- oder analysereihen
US6884626B1 (en) 1998-04-27 2005-04-26 Corning Incorporated Redrawn capillary imaging reservoir
US6350618B1 (en) 1998-04-27 2002-02-26 Corning Incorporated Redrawn capillary imaging reservoir
EP0955084B1 (de) * 1998-04-27 2006-07-26 Corning Incorporated Verfahren zur Ablage von biologischen Proben mit hilfe eines nachgezogenen Kapillar-speichers
US6048695A (en) * 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
GB9809943D0 (en) * 1998-05-08 1998-07-08 Amersham Pharm Biotech Ab Microfluidic device
GB9812783D0 (en) 1998-06-12 1998-08-12 Cenes Ltd High throuoghput screen
US6762061B1 (en) 1998-07-03 2004-07-13 Corning Incorporated Redrawn capillary imaging reservoir
DE69905049T2 (de) * 1998-07-13 2003-11-20 Cellomics, Inc. Auf zellen basiertes screening-system
US6238874B1 (en) * 1998-07-28 2001-05-29 Biometric Imaging, Inc. Cell motility assay
US6271042B1 (en) 1998-08-26 2001-08-07 Alpha Innotech Corporation Biochip detection system
WO2000017624A2 (en) * 1998-09-22 2000-03-30 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
DE19907011A1 (de) 1999-02-18 2000-08-31 Deutsches Krebsforsch Fluoreszenzkorrelationsspektroskopievorrichtung und -verfahren, insbesondere zur Mehrfarbenfluoreszenzkorrelationsspektroskopie
DE19913076A1 (de) * 1999-03-23 2000-10-19 Hahn Schickard Ges Vorrichtung und Verfahren zum Aufbringen von Mikrotröpfchen auf ein Substrat
DE19914279C1 (de) 1999-03-25 2000-09-07 Jena Optronik Gmbh Anordnung zum optischen Auslesen der Information von einem matrixförmigen Substrat mit einer Vielzahl von Einzelproben
US7932213B2 (en) 1999-05-11 2011-04-26 President And Fellows Of Harvard College Small molecule printing
US6743576B1 (en) 1999-05-14 2004-06-01 Cytokinetics, Inc. Database system for predictive cellular bioinformatics
US6651008B1 (en) 1999-05-14 2003-11-18 Cytokinetics, Inc. Database system including computer code for predictive cellular bioinformatics
US7151847B2 (en) 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
AU7131900A (en) * 1999-06-21 2001-01-09 Cellomics, Inc. A system for cell-based screening
WO2001007889A2 (en) * 1999-07-27 2001-02-01 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
AU6622800A (en) * 1999-08-05 2001-03-05 Cellomics, Inc. A system for cell-based screening
DE19941871A1 (de) 1999-09-02 2001-04-19 Hahn Schickard Ges Vorrichtung und Verfahren zum Aufbringen einer Mehrzahl von Mikrotröpfchen auf ein Substrat
WO2001027327A2 (en) 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
AU2622101A (en) * 1999-11-02 2001-05-14 Celine Hu Molecular microarrays and methods for production and use thereof
WO2001035072A2 (en) * 1999-11-09 2001-05-17 Cellomics, Inc. A system for cell-based screening
EP1144679A3 (de) * 1999-11-22 2002-02-06 Diversa Corporation Kapillar-anordnung und darauf basierte screeningverfahren
US6365367B1 (en) 1999-12-06 2002-04-02 Cellomics, Inc. Environmental chamber for the analysis of live cells
US6406840B1 (en) 1999-12-17 2002-06-18 Biomosaic Systems, Inc. Cell arrays and the uses thereof
AU2001234996A1 (en) 2000-02-11 2001-08-20 Yale University Planar patch clamp electrodes
DE10014204C2 (de) * 2000-03-22 2002-08-14 Max Planck Gesellschaft Mikrohybridisierungskammer
US6495337B1 (en) * 2000-03-29 2002-12-17 Cytokinetics, Inc. High throughput sarcomeric assay
DE10020704B4 (de) 2000-04-27 2006-09-28 Bioref Gmbh Biochip zur Archivierung und labormedizinischen Analyse von biologischem Probenmaterial, Verfahren zu dessen Herstellung sowie dessen Verwendung in diagnostischen Verfahren
US7546210B2 (en) 2000-06-08 2009-06-09 The Regents Of The University Of California Visual-servoing optical microscopy
EP1287114B1 (de) * 2000-06-08 2016-08-10 The Regents of The University of California Visuelle servosteuerung für optische mikroskopie
AU2007201501B2 (en) * 2000-06-08 2009-02-19 The Regents Of The University Of California Visual-servoing optical microscopy
US8548745B2 (en) 2000-06-08 2013-10-01 The Regents Of The University Of California Visual-servoing optical microscopy
DE10032730A1 (de) * 2000-07-05 2002-01-24 Nmi Univ Tuebingen Trägerplatte und Verfahren zur Durchführung funktioneller Tests
JP2004512833A (ja) * 2000-09-18 2004-04-30 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 単一細胞の選択部分の様々な流体成分での差別的な処理
GB2368903A (en) * 2000-11-08 2002-05-15 Proimmune Ltd Analysis of biological and biochemical assays
US6893836B2 (en) 2000-11-29 2005-05-17 Picoliter Inc. Spatially directed ejection of cells from a carrier fluid
US20020064808A1 (en) * 2000-11-29 2002-05-30 Mutz Mitchell W. Focused acoustic energy for ejecting cells from a fluid
US6849423B2 (en) 2000-11-29 2005-02-01 Picoliter Inc Focused acoustics for detection and sorting of fluid volumes
US7218764B2 (en) 2000-12-04 2007-05-15 Cytokinetics, Inc. Ploidy classification method
US6599694B2 (en) * 2000-12-18 2003-07-29 Cytokinetics, Inc. Method of characterizing potential therapeutics by determining cell-cell interactions
US6956961B2 (en) 2001-02-20 2005-10-18 Cytokinetics, Inc. Extracting shape information contained in cell images
US6999607B2 (en) 2001-02-20 2006-02-14 Cytokinetics, Inc. Method and apparatus for automated cellular bioinformatics
US7016787B2 (en) 2001-02-20 2006-03-21 Cytokinetics, Inc. Characterizing biological stimuli by response curves
CA2441206A1 (en) 2001-03-19 2002-09-26 Gyros Ab Characterization of reaction variables
DE60233542D1 (de) 2001-03-29 2009-10-15 Canon Kk Träger für Zellkulturen, seine Herstellung, Methode zur Kultivierung von Zellen
EP1248108B1 (de) * 2001-03-29 2011-08-31 Canon Kabushiki Kaisha Verfahren zum Zellscreening und dafür geeignete Substrata
GB0111004D0 (en) * 2001-05-04 2001-06-27 Univ Sheffield Screening method
EP1499705A2 (de) * 2002-05-01 2005-01-26 Massachusetts Institute of Technology Mikrofermentoren für schnelles screening und analyse von biochemischen prozessen
EP1599727B1 (de) * 2003-03-04 2008-05-14 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen Verfahren zur herstellung eines trägers mit immobilisierten viren und verwendung eines derartigen trägers
US7235353B2 (en) 2003-07-18 2007-06-26 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
EP1646926A2 (de) 2003-07-18 2006-04-19 Cytokinetics, Inc. Charakterisierung biologischer stimuli durch ansprechkurven
US20050014217A1 (en) 2003-07-18 2005-01-20 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
GB0317679D0 (en) 2003-07-29 2003-09-03 Amersham Biosciences Uk Ltd Analysing biological entities
JP4632400B2 (ja) 2003-12-16 2011-02-16 キヤノン株式会社 細胞培養用基板、その製造方法、それを用いた細胞スクリーニング法
US7419820B2 (en) 2003-12-16 2008-09-02 Canon Kabushiki Kaisha Transfer sheet for transferring biologically active substance to culture plate
JP2005323509A (ja) * 2004-05-12 2005-11-24 Inoue Keisuke 遺伝子発現計測用細胞培養観察装置
US7323318B2 (en) 2004-07-15 2008-01-29 Cytokinetics, Inc. Assay for distinguishing live and dead cells
US20070021433A1 (en) 2005-06-03 2007-01-25 Jian-Qiang Fan Pharmacological chaperones for treating obesity
EP1931979B1 (de) 2005-10-04 2015-11-18 Headway Technologies, Inc. Mikrofluidischer nachweis von analyten
US8114615B2 (en) 2006-05-17 2012-02-14 Cernostics, Inc. Method for automated tissue analysis
WO2011011350A2 (en) * 2009-07-20 2011-01-27 Siloam Biosciences, Inc. Microfluidic assay platforms
ES2739623T3 (es) 2011-03-17 2020-02-03 Cernostics Inc Sistemas y composiciones para diagnosticar el esófago de Barrett y métodos para usarlos
US9423234B2 (en) 2012-11-05 2016-08-23 The Regents Of The University Of California Mechanical phenotyping of single cells: high throughput quantitative detection and sorting
JP7109130B2 (ja) 2018-04-04 2022-07-29 株式会社エビデント 顕微鏡

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096807A (en) * 1985-03-06 1992-03-17 Murex Corporation Imaging immunoassay detection system with background compensation and its use
US4673988A (en) * 1985-04-22 1987-06-16 E.I. Du Pont De Nemours And Company Electronic mosaic imaging process
US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
SE462408B (sv) * 1988-11-10 1990-06-18 Pharmacia Ab Optiskt biosensorsystem utnyttjande ytplasmonresonans foer detektering av en specific biomolekyl, saett att kalibrera sensoranordningen samt saett att korrigera foer baslinjedrift i systemet
US5326691A (en) * 1991-11-21 1994-07-05 John Hozier Micro-libraries and methods of making and manipulating them methods for generating and analyzing micro-libraries
US5384261A (en) * 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths

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JP2000512009A (ja) 2000-09-12
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DE69738996D1 (de) 2008-10-30
EP0912892B1 (de) 2008-09-17

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