ATE417096T1 - Neue assays auf zellbasis für durch g-protein- gekoppelte rezeptoren vermittelte aktivitäten - Google Patents

Neue assays auf zellbasis für durch g-protein- gekoppelte rezeptoren vermittelte aktivitäten

Info

Publication number
ATE417096T1
ATE417096T1 AT02802463T AT02802463T ATE417096T1 AT E417096 T1 ATE417096 T1 AT E417096T1 AT 02802463 T AT02802463 T AT 02802463T AT 02802463 T AT02802463 T AT 02802463T AT E417096 T1 ATE417096 T1 AT E417096T1
Authority
AT
Austria
Prior art keywords
protein
coupled receptors
assays
based assays
new cell
Prior art date
Application number
AT02802463T
Other languages
English (en)
Inventor
Yong Yao
Liang Cao
Original Assignee
Atto Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atto Bioscience Inc filed Critical Atto Bioscience Inc
Application granted granted Critical
Publication of ATE417096T1 publication Critical patent/ATE417096T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT02802463T 2001-10-26 2002-10-25 Neue assays auf zellbasis für durch g-protein- gekoppelte rezeptoren vermittelte aktivitäten ATE417096T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33066301P 2001-10-26 2001-10-26
US10/087,217 US7115377B2 (en) 2001-10-26 2002-03-04 Cell-based assays for G-protein-coupled receptor-mediated activities

Publications (1)

Publication Number Publication Date
ATE417096T1 true ATE417096T1 (de) 2008-12-15

Family

ID=26776733

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02802463T ATE417096T1 (de) 2001-10-26 2002-10-25 Neue assays auf zellbasis für durch g-protein- gekoppelte rezeptoren vermittelte aktivitäten

Country Status (8)

Country Link
US (3) US7115377B2 (de)
EP (1) EP1444331B1 (de)
JP (1) JP4324474B2 (de)
AT (1) ATE417096T1 (de)
AU (1) AU2002363225A1 (de)
DE (1) DE60230313D1 (de)
DK (1) DK1444331T3 (de)
WO (1) WO2003038039A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115377B2 (en) * 2001-10-26 2006-10-03 Atto Bioscience, Inc. Cell-based assays for G-protein-coupled receptor-mediated activities
US7238213B2 (en) 2001-10-26 2007-07-03 Atto Bioscience Cell-based assays employing voltage and calcium dyes
US7166463B2 (en) * 2001-11-16 2007-01-23 The Regents Of The University Of Colorado Nucleic acids encoding modified olfactory cyclic nucleotide gated ion channels
JP4429577B2 (ja) * 2002-09-30 2010-03-10 株式会社ニコン 細胞内反応測定装置および細胞内反応測定方法
DE10311769A1 (de) * 2003-03-18 2004-09-30 Bayer Healthcare Ag Testmethoden zur Bestimmung der intrazellulären Konzentration zyklischer Nukleotide
EP1682577B1 (de) * 2003-10-30 2012-04-18 Atto Bioscience, Inc. Assays auf zellbasis unter anwendung von spannung und calciumfarbstoffen
US8329390B2 (en) * 2004-10-21 2012-12-11 Anaspec Incorporated Detection of transmembrane potentials using N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonols
GB2456236B8 (en) * 2007-03-22 2009-12-09 Heptares Therapeutics Ltd Stable muscarinic receptor mutants
GB0724051D0 (en) * 2007-12-08 2008-01-16 Medical Res Council Mutant proteins and methods for producing them
GB0724860D0 (en) 2007-12-20 2008-01-30 Heptares Therapeutics Ltd Screening
GB0802474D0 (en) * 2008-02-11 2008-03-19 Heptares Therapeutics Ltd Mutant proteins and methods for selecting them
WO2008068534A2 (en) * 2008-03-05 2008-06-12 Heptares Therapeutics Limited Crystal structure of a betal -adremergi c receptor and uses thereof
WO2010011407A2 (en) * 2008-05-23 2010-01-28 President And Fellows Of Harvard College Methods of generating patterned soft substrates and uses thereof
GB0910725D0 (en) 2009-06-22 2009-08-05 Heptares Therapeutics Ltd Mutant proteins and methods for producing them
US20180080929A1 (en) * 2015-03-24 2018-03-22 National University Corporation Nagoya University Analytical device
WO2017087759A1 (en) 2015-11-18 2017-05-26 President And Fellows Of Harvard College Cartridge-based system for long term culture of cell clusters
US11629318B2 (en) 2017-10-20 2023-04-18 President And Fellows Of Harvard College Methods for producing mature adipocytes and methods of use thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0988395A2 (de) 1997-06-13 2000-03-29 Allelix Biopharmaceuticals Inc. Nachweisverfahren und zusammensetzungen um rezeptor-ligand bindungen zu messen
CA2255548A1 (en) 1998-12-14 2000-06-14 Allelix Biopharmaceuticals Inc. Assay methods and compositions useful for measuring receptor ligand binding
US7115377B2 (en) * 2001-10-26 2006-10-03 Atto Bioscience, Inc. Cell-based assays for G-protein-coupled receptor-mediated activities
US7166463B2 (en) * 2001-11-16 2007-01-23 The Regents Of The University Of Colorado Nucleic acids encoding modified olfactory cyclic nucleotide gated ion channels

Also Published As

Publication number Publication date
US7384755B2 (en) 2008-06-10
DK1444331T3 (da) 2009-04-06
WO2003038039A3 (en) 2004-03-18
US7897386B2 (en) 2011-03-01
AU2002363225A1 (en) 2003-05-12
EP1444331A4 (de) 2005-03-02
US7115377B2 (en) 2006-10-03
EP1444331A2 (de) 2004-08-11
US20030100059A1 (en) 2003-05-29
EP1444331B1 (de) 2008-12-10
WO2003038039A2 (en) 2003-05-08
JP2005507667A (ja) 2005-03-24
DE60230313D1 (de) 2009-01-22
US20090087906A1 (en) 2009-04-02
US20070178483A1 (en) 2007-08-02
JP4324474B2 (ja) 2009-09-02

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