ATE419384T1 - Durchfluss-mikroreaktor in dem lokale temperaturzyklen auf eine fliessende probe einwirken - Google Patents

Durchfluss-mikroreaktor in dem lokale temperaturzyklen auf eine fliessende probe einwirken

Info

Publication number
ATE419384T1
ATE419384T1 AT00949852T AT00949852T ATE419384T1 AT E419384 T1 ATE419384 T1 AT E419384T1 AT 00949852 T AT00949852 T AT 00949852T AT 00949852 T AT00949852 T AT 00949852T AT E419384 T1 ATE419384 T1 AT E419384T1
Authority
AT
Austria
Prior art keywords
local temperature
temperature cycles
sample
flowing sample
flow microreactor
Prior art date
Application number
AT00949852T
Other languages
English (en)
Inventor
Yves Fouillet
Claude Vauchier
Jean-Frederic Clerc
Christine Peponnet
Original Assignee
Merck Serono Biodevelopment
Commissariat Energie Atomique
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9909806A external-priority patent/FR2796863B1/fr
Priority claimed from FR9911652A external-priority patent/FR2798604B1/fr
Priority claimed from FR9912317A external-priority patent/FR2799139B1/fr
Application filed by Merck Serono Biodevelopment, Commissariat Energie Atomique filed Critical Merck Serono Biodevelopment
Application granted granted Critical
Publication of ATE419384T1 publication Critical patent/ATE419384T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0845—Filaments, strings, fibres, i.e. not hollow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1883—Means for temperature control using thermal insulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/02—Water baths; Sand baths; Air baths
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F2260/00—Heat exchangers or heat exchange elements having special size, e.g. microstructures
    • F28F2260/02—Heat exchangers or heat exchange elements having special size, e.g. microstructures having microchannels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Optical Measuring Cells (AREA)
AT00949852T 1999-07-28 2000-07-28 Durchfluss-mikroreaktor in dem lokale temperaturzyklen auf eine fliessende probe einwirken ATE419384T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9909806A FR2796863B1 (fr) 1999-07-28 1999-07-28 Procede et dispositif permettant de realiser en flux continu un protocole de traitement thermique sur une substance
FR9911652A FR2798604B1 (fr) 1999-09-17 1999-09-17 Dispositif pour la realisation de reactions chimiques ou biochimiques par cyclage thermique
FR9912317A FR2799139B1 (fr) 1999-10-01 1999-10-01 Dispositif d'analyse biochimique comprenant un substrat microfluidique notamment pour l'amplification ou l'analyse d'acides nucleiques.

Publications (1)

Publication Number Publication Date
ATE419384T1 true ATE419384T1 (de) 2009-01-15

Family

ID=27253548

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00949852T ATE419384T1 (de) 1999-07-28 2000-07-28 Durchfluss-mikroreaktor in dem lokale temperaturzyklen auf eine fliessende probe einwirken

Country Status (8)

Country Link
US (2) US7015030B1 (de)
EP (1) EP1203096B1 (de)
JP (1) JP2003505059A (de)
AT (1) ATE419384T1 (de)
AU (1) AU782726B2 (de)
CA (1) CA2379927A1 (de)
DE (1) DE60041264D1 (de)
WO (1) WO2001007159A2 (de)

Families Citing this family (114)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6977145B2 (en) * 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US8900811B2 (en) * 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
FR2822848B1 (fr) * 2001-03-28 2004-05-07 Genesystems Dispositif integre et miniature de sequencage d'acides nucleiques
US20030032172A1 (en) * 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
AUPR707101A0 (en) * 2001-08-16 2001-09-06 Corbett Research Pty Ltd Continuous flow thermal device
US20030108664A1 (en) * 2001-10-05 2003-06-12 Kodas Toivo T. Methods and compositions for the formation of recessed electrical features on a substrate
FR2831081B1 (fr) * 2001-10-24 2004-09-03 Commissariat Energie Atomique Dispositif d'injection parallelisee et synchronisee pour injections sequentielles de reactifs differents
US7094379B2 (en) 2001-10-24 2006-08-22 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
AU2003283663A1 (en) * 2002-11-01 2004-05-25 Cellectricon Ab Computer programs,workstations, systems and methods for microfluidic substrates in cell
DE10258398A1 (de) * 2002-12-11 2004-08-12 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Verfahren zur Amplifikation von DNA
WO2004061085A2 (en) * 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
JP2004305009A (ja) * 2003-04-02 2004-11-04 Hitachi Ltd 核酸増幅装置及び核酸増幅方法
US8293471B2 (en) * 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
KR100552706B1 (ko) * 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
EP1598429A1 (de) * 2004-05-19 2005-11-23 Amplion Ltd. Detektion von Amplikon-Kontamination während einer PCR, die zwei verschiedene Annealing-Temperaturen aufweist
JP4482929B2 (ja) * 2004-06-01 2010-06-16 富士フイルム株式会社 科学現象の評価装置の製造方法
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
WO2006032044A2 (en) 2004-09-15 2006-03-23 Microchip Biotechnologies, Inc. Microfluidic devices
WO2006046433A1 (ja) * 2004-10-27 2006-05-04 Konica Minolta Medical & Graphic, Inc. 遺伝子検査用マイクロリアクタ
US7727477B2 (en) * 2004-12-10 2010-06-01 Bio-Rad Laboratories, Inc. Apparatus for priming microfluidics devices with feedback control
EP2339320B1 (de) * 2005-01-25 2012-06-20 Oscillogy LLC Temperaturregler für kleine Flüssigkeitsproben mit unterschiedlichen Wärmekapazitäten
WO2006100812A1 (ja) * 2005-03-23 2006-09-28 Fujifilm Corporation 科学現象評価装置の実験方法、及び科学現象評価装置
JP4501158B2 (ja) * 2005-03-30 2010-07-14 富士フイルム株式会社 マイクロ化学装置の運転方法
KR100714988B1 (ko) * 2005-08-09 2007-05-09 삼성전자주식회사 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한ｄｎａ 정제 방법 및 정제장치
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
CA2641271A1 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US20080280331A1 (en) 2006-02-07 2008-11-13 Stokes Bio Limited Microfluidic Analysis System
US7766033B2 (en) * 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7816121B2 (en) * 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
US8389297B2 (en) 2006-04-18 2013-03-05 Duke University Droplet-based affinity assay device and system
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
WO2007142061A1 (ja) * 2006-06-06 2007-12-13 Konica Minolta Medical & Graphic, Inc. マイクロチップ検査装置
US7500379B2 (en) * 2006-06-26 2009-03-10 Applied Sensor Research & Development Corporation Acoustic wave array chemical and biological sensor
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
EP1876453A1 (de) * 2006-07-07 2008-01-09 Agilent Technologies, Inc. Temperatursteuerung für integrierten Chip
WO2008045288A2 (en) * 2006-10-06 2008-04-17 Vandalia Research, Inc. Method for a continuous rapid thermo cycle system
US8841116B2 (en) * 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US9114398B2 (en) * 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
KR20100028526A (ko) 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. 마이크로유체 및 나노유체 장치, 시스템 및 응용
CA2691197C (en) 2007-06-21 2013-03-12 Gen-Probe Incorporated Instrument and receptacles for use in performing processes
US8454906B2 (en) * 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US8380457B2 (en) * 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
JP5224801B2 (ja) * 2007-12-21 2013-07-03 キヤノン株式会社 核酸増幅装置
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
FR2930457B1 (fr) * 2008-04-24 2010-06-25 Commissariat Energie Atomique Procede de fabrication de microcanaux reconfigurables
DE102008040062A1 (de) * 2008-07-02 2010-01-07 Robert Bosch Gmbh Verfahren zum Betreiben eines Fluidventils mit einer oszillierenden Ventilbewegung
CN102405402A (zh) 2008-09-23 2012-04-04 阔达生命有限公司 基于液滴的测定系统
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
EP2384429A1 (de) 2008-12-31 2011-11-09 Integenx Inc. Instrument mit mikrofluidischem chip
CN102439130A (zh) 2009-03-31 2012-05-02 财团法人神奈川科学技术研究院 液体回流型高速基因扩增装置
CN102459565A (zh) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 具有隔膜阀的流控设备
EP2438016B1 (de) 2009-06-05 2021-06-02 IntegenX Inc. Universelles probenpräparationssystem und seine verwendung in einem integrierten analysesystem
US20110001963A1 (en) * 2009-07-02 2011-01-06 Durack Gary P System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system
WO2011005781A1 (en) * 2009-07-06 2011-01-13 Sony Corporation Microfluidic device
TW201105971A (en) * 2009-07-06 2011-02-16 Sony Corp Microfluidic device having onboard tissue or cell sample handling capability
TW201109653A (en) * 2009-07-06 2011-03-16 Sony Corp Microfluidic device
CN102482631A (zh) 2009-07-07 2012-05-30 索尼公司 适于在离心处理后选择性地提取样品的微流体装置及其使用方法
WO2011005776A1 (en) * 2009-07-07 2011-01-13 Sony Corporation Microfluidic device
WO2011005754A1 (en) * 2009-07-08 2011-01-13 Sony Corporation Microfluidic device having a flow channel within a gain medium
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
EP2550528B1 (de) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Tropfenerzeugung für tropfenbasierte prüfungen
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP2606242A4 (de) 2010-08-20 2016-07-20 Integenx Inc Mikrofluidikvorrichtungen mit mechanisch abgedichteten membranventilen
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
WO2012027391A1 (en) * 2010-08-23 2012-03-01 Life Technologies Corporation Temperature control of chemical detection system
CA2816712C (en) 2010-11-01 2018-12-11 Donald A. Masquelier System for forming emulsions
PL2640823T3 (pl) * 2010-11-17 2019-04-30 Diagcor Life Science Ltd Urządzenie przepływowe
EP2646154A2 (de) * 2010-11-30 2013-10-09 Quantumdx Group Limited Entwurf, herstellung und verwendung einer flexiblen multitemperatur-reaktionsvorrichtung für die mikrofluidik
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
EP2702175B1 (de) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
DE102011017596A1 (de) 2011-04-27 2012-10-31 Robert Bosch Gmbh Mikrofluidisches System und Verfahren für eine Polymerase Kettenreaktion
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
JP6027321B2 (ja) * 2012-03-06 2016-11-16 公益財団法人神奈川科学技術アカデミー 高速遺伝子増幅検出装置
JPWO2013141331A1 (ja) * 2012-03-22 2015-08-03 和光純薬工業株式会社 マイクロサテライト領域を有するｄｎａの検出方法
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
WO2015054188A1 (en) 2013-10-07 2015-04-16 Apdn (B.V.I), Inc. Multimode image and spectral reader
CN105873681B (zh) 2013-11-18 2019-10-11 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
CN106103121B (zh) 2014-03-18 2019-12-06 亚普蒂恩(B.V.I.)公司 用于安全应用的加密光学标记物
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
CN113092563B (zh) 2014-10-22 2024-06-07 尹特根埃克斯有限公司 用于样品制备、处理和分析的系统和方法
EP3313978B1 (de) * 2016-01-08 2024-02-28 Hewlett-Packard Development Company, L.P. Polymerase-kettenreaktionsvorrichtung
CN109070130B (zh) 2016-04-11 2022-03-22 亚普蒂恩（B V I）公司 用于标记纤维素产品的方法
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
WO2018156352A1 (en) 2017-02-21 2018-08-30 Apdn (B.V.I) Inc. Nucleic acid coated submicron particles for authentication
DE102017204705A1 (de) * 2017-03-21 2018-09-27 Robert Bosch Gmbh Stoffgemisch und Verwendung eines Stoffgemischs für eine Polymerase-Kettenreaktion
WO2019103730A1 (en) 2017-11-22 2019-05-31 Hewlett-Packard Development Company, L.P. Temperature-controlling microfluidic devices
CN108300656A (zh) * 2018-02-05 2018-07-20 冷彦宁 一种热介质流动式pcr扩增的方法
TWI708842B (zh) * 2019-10-02 2020-11-01 中原大學 仿生器官裝置以及其形成方法
WO2025111528A1 (en) * 2023-11-22 2025-05-30 Idex Health & Science Llc In-line reaction system with gas removal
WO2025229120A1 (en) * 2024-04-30 2025-11-06 Qiagen Gmbh Digital polymerase chain reaction, pcr, device, microfluidic device, system, and method of heating a sample in a digital pcr device

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2565350B1 (fr) * 1984-06-05 1986-10-10 Paris Nord Universite Moyens propres a permettre le support, le traitement, le stockage et l'analyse automatiques en continu d'echantillons biologiques
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
WO1993022058A1 (en) 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Polynucleotide amplification analysis using a microfabricated device
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
DE69429038T2 (de) * 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US5580728A (en) 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
US5508197A (en) * 1994-07-25 1996-04-16 The Regents, University Of California High-speed thermal cycling system and method of use
DE4435107C1 (de) 1994-09-30 1996-04-04 Biometra Biomedizinische Analy Miniaturisierter Fluß-Thermocycler
JP3599390B2 (ja) 1994-11-04 2004-12-08 日本メクトロン株式会社 パーフルオロ不飽和ニトリル化合物およびその製造法
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
WO1996015269A2 (en) * 1994-11-14 1996-05-23 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
WO1996028412A1 (en) 1995-03-10 1996-09-19 Photocure As Esters of 5-aminolevulinic acid as photosensitizing agents in photochemotherapy
US5589136A (en) 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US5795976A (en) 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
AU1115697A (en) 1995-11-03 1997-05-22 Sarnoff Corporation Assay system and method for conducting assays
US5736314A (en) 1995-11-16 1998-04-07 Microfab Technologies, Inc. Inline thermo-cycler
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6054277A (en) 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6136212A (en) * 1996-08-12 2000-10-24 The Regents Of The University Of Michigan Polymer-based micromachining for microfluidic devices
EP0946749A1 (de) 1996-11-20 1999-10-06 The Regents Of The University Of Michigan Mikrofabrizierte vorrichtungen sowie verfahren zur isothermen amplifikation von nukleinsäuren
SE9700205D0 (sv) 1997-01-24 1997-01-24 Peter Lindberg Integrated microfluidic element
AU746892B2 (en) 1997-04-04 2002-05-02 Caliper Life Sciences, Inc. Closed-loop biochemical analyzers
DE19717085C2 (de) * 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Verfahren und Geräte für extrem schnelle DNA-Vervielfachung durch Polymerase-Kettenreaktionen (PCR)
AU746098B2 (en) 1997-09-02 2002-04-18 Caliper Life Sciences, Inc. Microfluidic system with electrofluidic and electrothermal controls
US6210882B1 (en) 1998-01-29 2001-04-03 Mayo Foundation For Medical Education And Reseach Rapid thermocycling for sample analysis
US6896855B1 (en) 1998-02-11 2005-05-24 Institut Fuer Physikalische Hochtechnologie E.V. Miniaturized temperature-zone flow reactor
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
WO2000023190A1 (fr) * 1998-10-16 2000-04-27 Commissariat A L'energie Atomique Dispositif d'analyse chimique et/ou biochimique avec un support d'analyse
US6977145B2 (en) * 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor

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