ATE420208T1 - Verbesserte koamplifikation von nukleinsäuren - Google Patents
Verbesserte koamplifikation von nukleinsäurenInfo
- Publication number
- ATE420208T1 ATE420208T1 AT03021423T AT03021423T ATE420208T1 AT E420208 T1 ATE420208 T1 AT E420208T1 AT 03021423 T AT03021423 T AT 03021423T AT 03021423 T AT03021423 T AT 03021423T AT E420208 T1 ATE420208 T1 AT E420208T1
- Authority
- AT
- Austria
- Prior art keywords
- target nucleic
- primer extension
- nucleic acids
- reaction mixture
- temperature
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 11
- 150000007523 nucleic acids Chemical class 0.000 title abstract 11
- 102000039446 nucleic acids Human genes 0.000 title abstract 11
- 230000003321 amplification Effects 0.000 abstract 8
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 8
- 239000011541 reaction mixture Substances 0.000 abstract 6
- 230000007717 exclusion Effects 0.000 abstract 5
- 239000003153 chemical reaction reagent Substances 0.000 abstract 4
- 239000003795 chemical substances by application Substances 0.000 abstract 4
- 230000037452 priming Effects 0.000 abstract 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 239000012807 PCR reagent Substances 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 238000001816 cooling Methods 0.000 abstract 2
- 230000036425 denaturation Effects 0.000 abstract 2
- 238000004925 denaturation Methods 0.000 abstract 2
- 238000010438 heat treatment Methods 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- 238000011534 incubation Methods 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20020021174 EP1403379A1 (de) | 2002-09-24 | 2002-09-24 | Verbesserte Koamplifikation von Nukleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE420208T1 true ATE420208T1 (de) | 2009-01-15 |
Family
ID=31970287
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03021423T ATE420208T1 (de) | 2002-09-24 | 2003-09-23 | Verbesserte koamplifikation von nukleinsäuren |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8067206B2 (de) |
| EP (1) | EP1403379A1 (de) |
| JP (1) | JP4675558B2 (de) |
| AT (1) | ATE420208T1 (de) |
| DE (1) | DE60325679D1 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| WO2005118853A2 (en) * | 2004-06-01 | 2005-12-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| GB2424886A (en) * | 2005-04-04 | 2006-10-11 | Dxs Ltd | Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations |
| EP2829615B1 (de) | 2005-07-25 | 2018-05-09 | Alere San Diego, Inc. | Kit zur Multiplexierung einer Rekombinase-Polymerase-Verstärkung |
| WO2007032837A2 (en) * | 2005-08-11 | 2007-03-22 | The J. Craig Venter Institute | Method for in vitro recombination |
| DK1915446T3 (en) | 2005-08-11 | 2017-09-11 | Synthetic Genomics Inc | IN VITRO RECOMBINATION PROCEDURE |
| EP2029782B1 (de) | 2006-05-04 | 2014-11-26 | Alere San Diego, Inc. | Rekombinase-polymerase-verstärkung |
| EP2077337A1 (de) * | 2007-12-26 | 2009-07-08 | Eppendorf Array Technologies SA | Zusammensetzung, Verfahren und Kit zum Nachweis und zur Amplifikation |
| JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質 |
| EP2369325A1 (de) * | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Arrayanalyse für Online-Detektion |
| US9587268B2 (en) * | 2014-01-29 | 2017-03-07 | Agilent Technologies Inc. | Fast hybridization for next generation sequencing target enrichment |
| ES2899826T3 (es) | 2015-06-04 | 2022-03-14 | Mizuho Medy Co Ltd | Kit para detectar conjuntamente múltiples ácidos nucleicos diana que difieren entre sí y procedimiento de detección usando el mismo |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2555589B1 (fr) * | 1983-11-30 | 1986-05-16 | Choay Sa | Nouveaux derives du dextrane a activites anticoagulante en anti-inflammatoire, leur procede de preparation et utilisation de ces derives en tant qu'anticoagulants et en tant que substituts du plasma sanguin |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4902624A (en) | 1987-11-23 | 1990-02-20 | Eastman Kodak Company | Temperature cycling cuvette |
| US5459038A (en) * | 1988-01-29 | 1995-10-17 | Advanced Riverina Holdings, Ltd. | Determination of genetic sex in ruminants using Y-chromosome specific polynucleotides |
| US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| US5229297A (en) | 1989-02-03 | 1993-07-20 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
| US5089233A (en) | 1989-06-12 | 1992-02-18 | Eastman Kodak Company | Processing apparatus for a chemical reaction pack |
| US5106730A (en) | 1989-07-24 | 1992-04-21 | Microprobe Corporation | Lactam-containing compositions and methods useful for the hybridization of nucleic acids |
| CA2075050C (en) | 1990-02-16 | 1998-10-06 | Will Bloch | Specificity and convenience of the polymerase chain reaction |
| US5194370A (en) | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
| US5173260A (en) | 1990-09-17 | 1992-12-22 | Eastman Kodak Company | Beads fused to a test device support |
| US5705366A (en) * | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US6183998B1 (en) * | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| EP1111044A4 (de) * | 1998-09-08 | 2003-06-04 | Takara Shuzo Co | Methode zur dna-synthese |
| JP4186269B2 (ja) * | 1998-09-21 | 2008-11-26 | 株式会社島津製作所 | 核酸合成法 |
| EP0989192A3 (de) * | 1998-09-21 | 2004-02-25 | Shimadzu Corporation | Verfahren zur Nucleinsäuresynthese |
-
2002
- 2002-09-24 EP EP20020021174 patent/EP1403379A1/de not_active Withdrawn
-
2003
- 2003-09-23 DE DE60325679T patent/DE60325679D1/de not_active Expired - Lifetime
- 2003-09-23 AT AT03021423T patent/ATE420208T1/de not_active IP Right Cessation
- 2003-09-24 US US10/669,976 patent/US8067206B2/en not_active Expired - Lifetime
- 2003-09-24 JP JP2003332155A patent/JP4675558B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20040115712A1 (en) | 2004-06-17 |
| DE60325679D1 (de) | 2009-02-26 |
| EP1403379A1 (de) | 2004-03-31 |
| US8067206B2 (en) | 2011-11-29 |
| JP2004113242A (ja) | 2004-04-15 |
| JP4675558B2 (ja) | 2011-04-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |