ATE425266T1 - Aptamer-nanopartikelkonjugate und verwendungsverfahren zur detektion von zielanalyten - Google Patents

Aptamer-nanopartikelkonjugate und verwendungsverfahren zur detektion von zielanalyten

Info

Publication number
ATE425266T1
ATE425266T1 AT05779974T AT05779974T ATE425266T1 AT E425266 T1 ATE425266 T1 AT E425266T1 AT 05779974 T AT05779974 T AT 05779974T AT 05779974 T AT05779974 T AT 05779974T AT E425266 T1 ATE425266 T1 AT E425266T1
Authority
AT
Austria
Prior art keywords
aptamer
methods
target analytes
detecting target
nanoparticle conjugates
Prior art date
Application number
AT05779974T
Other languages
English (en)
Inventor
Uwe Mueller
James Storhoff
Michael Senical
Viswanadham Garimella
Original Assignee
Nanosphere Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanosphere Inc filed Critical Nanosphere Inc
Application granted granted Critical
Publication of ATE425266T1 publication Critical patent/ATE425266T1/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nanotechnology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medical Informatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
AT05779974T 2004-05-03 2005-05-03 Aptamer-nanopartikelkonjugate und verwendungsverfahren zur detektion von zielanalyten ATE425266T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56787404P 2004-05-03 2004-05-03
US10/995,051 US20050250094A1 (en) 2003-05-30 2004-11-22 Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes

Publications (1)

Publication Number Publication Date
ATE425266T1 true ATE425266T1 (de) 2009-03-15

Family

ID=35428943

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05779974T ATE425266T1 (de) 2004-05-03 2005-05-03 Aptamer-nanopartikelkonjugate und verwendungsverfahren zur detektion von zielanalyten

Country Status (8)

Country Link
US (2) US20050250094A1 (de)
EP (1) EP1766085B1 (de)
JP (1) JP2007536528A (de)
AT (1) ATE425266T1 (de)
AU (2) AU2005245824A1 (de)
CA (1) CA2564217A1 (de)
DE (1) DE602005013225D1 (de)
WO (1) WO2005113817A2 (de)

Families Citing this family (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US8152991B2 (en) 2005-10-27 2012-04-10 Nanomix, Inc. Ammonia nanosensors, and environmental control system
US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US7714398B2 (en) * 2002-09-05 2010-05-11 Nanomix, Inc. Nanoelectronic measurement system for physiologic gases and improved nanosensor for carbon dioxide
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
WO2005026694A2 (en) * 2003-09-12 2005-03-24 Nanomix, Inc. Carbon dioxide nanoelectronic sensor
CA2834041C (en) * 2003-12-31 2017-05-16 President And Fellows Of Harvard College Assay device and method
JP2008507296A (ja) * 2004-07-26 2008-03-13 ナノスフェアー　インコーポレイテッド 混合培養物中でメチシリン耐性黄色ブドウ球菌とメチシリン感受性黄色ブドウ球菌とを区別する方法
WO2007044025A2 (en) * 2004-11-22 2007-04-19 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nonoparticle probe complexes
US20070026391A1 (en) * 2005-04-11 2007-02-01 Ghc Technologies, Inc. Methods and compositions for identifying chemical or biological agents using multiplexed labeling and colocalization detection
WO2006125050A2 (en) * 2005-05-17 2006-11-23 Nanosphere, Inc. Biobarcode assays for ultra high sensitive detection
EP1915466B1 (de) * 2005-08-19 2010-11-24 Nanosphere, Inc. Verfahren zur herstellung von hybridsubstraten mit dna und antikörpern sowie verwendungen davon
US20080019659A1 (en) * 2005-10-26 2008-01-24 Xiaoming Tao Photonic fabric display with controlled graphic pattern, color, luminescence intensity, and light self-amplification
US7466896B2 (en) 2005-10-26 2008-12-16 The Hong Kong Polytechnic University Photonic fabric display with controlled pattern, color, luminescence intensity, scattering intensity and light self-amplification
EP2644615B1 (de) * 2006-02-08 2017-04-05 Becton Dickinson and Company SERS-Nanoetikett-Assays
JP2009532064A (ja) * 2006-04-04 2009-09-10 ナノミックス・インコーポレーテッド 被検体増幅とレポーターを用いる生体分子のナノ電子的検出
WO2007133704A2 (en) * 2006-05-11 2007-11-22 Nanosphere, Inc. A non-nucleic acid based biobarcode assay for detection of biological materials
WO2007146162A2 (en) * 2006-06-08 2007-12-21 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Devices, systems and methods for reducing the concentration of a chemical entity in fluids
JP4878223B2 (ja) 2006-06-16 2012-02-15 国立大学法人東京農工大学 核酸と蛋白質の相互作用の検出方法及び装置
WO2008016988A1 (en) * 2006-08-01 2008-02-07 Gen-Probe Incorporated Methods of nonspecific target capture of nucleic acids
KR100828936B1 (ko) 2006-08-01 2008-05-20 김성천 단일가닥핵산 압타머와 금-나노입자를 이용한 생체분자분석방법
US20100099858A1 (en) * 2006-09-28 2010-04-22 Mirkin Chad A Maximizing Oligonucleotide Loading on Gold Nanoparticle
WO2008147394A1 (en) * 2006-11-08 2008-12-04 Northeastern University Three dimensional nanoscale circuit interconnect and method of assembly by dielectrophoresis
US9589686B2 (en) 2006-11-16 2017-03-07 General Electric Company Apparatus for detecting contaminants in a liquid and a system for use thereof
US9658178B2 (en) 2012-09-28 2017-05-23 General Electric Company Sensor systems for measuring an interface level in a multi-phase fluid composition
US9536122B2 (en) 2014-11-04 2017-01-03 General Electric Company Disposable multivariable sensing devices having radio frequency based sensors
US10914698B2 (en) 2006-11-16 2021-02-09 General Electric Company Sensing method and system
US9538657B2 (en) 2012-06-29 2017-01-03 General Electric Company Resonant sensor and an associated sensing method
US20100134286A1 (en) * 2008-12-01 2010-06-03 General Electric Company Radio frequency based sensors employing analyte recognition element
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
AU2008212820B2 (en) * 2007-02-09 2014-01-30 Northwestern University Particles for detecting intracellular targets
JP5238171B2 (ja) * 2007-03-14 2013-07-17 株式会社東芝 光導波路型抗体チップ
WO2008150873A1 (en) * 2007-05-31 2008-12-11 Purdue Research Foundation Ultrasensitive detection of biomolecules using immunoseparation and diffractometry
US20100105079A1 (en) * 2007-06-01 2010-04-29 Atonomics A/S Bio surface acoustic wave (saw) resonator amplification with nanoparticles for detection of a target analyte
US20110059431A1 (en) * 2007-06-18 2011-03-10 Northwestern University Non-enzymatic detection of bacterial genomic dna
JP4947520B2 (ja) * 2007-07-04 2012-06-06 国立大学法人東京農工大学 生体小分子の検出方法及び装置
US7973019B1 (en) 2007-10-03 2011-07-05 Alcon Research, Ltd. Transferrin/transferrin receptor-mediated siRNA delivery
US20090131358A1 (en) * 2007-11-15 2009-05-21 Alcon Research, Ltd. LOW DENSITY LIPOPROTEIN RECEPTOR-MEDIATED siRNA DELIVERY
WO2009076323A2 (en) 2007-12-06 2009-06-18 Genalyte Inc. Monitoring enzymatic process
US20110065086A1 (en) * 2008-02-21 2011-03-17 Otc Biotechnologies, Llc Methods of producing homogeneous plastic-adherent aptamer-magnetic bead-fluorophore and other sandwich assays
JP5588970B2 (ja) 2008-05-27 2014-09-10 ダコ・デンマーク・エー／エス ハイブリダイゼーション組成物及び方法
EP2347247B1 (de) 2008-10-27 2019-06-26 Genalyte, Inc. Auf optischer sondierung und optischem abtasten basierende biosensoren
CN102227625A (zh) * 2008-12-02 2011-10-26 皇家飞利浦电子股份有限公司 用于通过受抑全内反射来探测目标颗粒的传感器装置
US7879625B1 (en) 2008-12-03 2011-02-01 The United States Of America As Represented By The Secretary Of The Navy Preparation of SERS substrates on silica-coated magnetic microspheres
JP5260339B2 (ja) * 2009-01-30 2013-08-14 株式会社日立ハイテクノロジーズ 核酸分析デバイス、及び核酸分析装置
US9303287B2 (en) 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
KR101090982B1 (ko) 2009-03-16 2011-12-08 경희대학교 산학협력단 양자점-앱타머 접합체를 이용한 생분석 방법
GB0904934D0 (en) 2009-03-23 2009-05-06 Geneseqe As Method and apparatus for detecting molecules
US8993346B2 (en) 2009-08-07 2015-03-31 Nanomix, Inc. Magnetic carbon nanotube based biodetection
EP2504436A1 (de) 2009-11-23 2012-10-03 INSERM - Institut National de la Santé et de la Recherche Médicale Gegen matrixprotein-1 von typ-a-influenzaviren gerichtete aptamere und ihre verwendungen
US20110158901A1 (en) * 2009-12-29 2011-06-30 Swadeshmukul Santra Chitosan-based nanoparticles and methods for making and using the same
US9005994B2 (en) 2010-01-14 2015-04-14 University Of Central Florida Research Foundation, Inc. Methods for biomolecule and biomolecule complex (BMC) detection and analysis and the use of such for research and medical diagnosis
KR101198476B1 (ko) * 2010-08-31 2012-11-06 연세대학교 산학협력단 나노 구조 기반의 초고해상도 영상 방법 및 장치
CN101967517B (zh) * 2010-03-19 2012-11-07 黄乐群 一种无需借助pcr的基因检测方法
CN102269758B (zh) * 2010-06-04 2013-11-20 国家纳米科学中心 可视化检测抗原抗体反应的方法、试剂盒及其应用
WO2012004790A2 (en) * 2010-07-06 2012-01-12 Aptateck Bio Ltd. Nucleic acid aptamer-based diagnostic methods with novel techniques for signal enhancement
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
EP2635710B1 (de) * 2010-11-05 2017-08-09 Genalyte, Inc. Optische analytnachweissysteme und anwendungsverfahren dafür
US8542023B2 (en) 2010-11-09 2013-09-24 General Electric Company Highly selective chemical and biological sensors
US9353421B2 (en) 2011-03-31 2016-05-31 Helmholtz-Zentrum für Umweltforschung GmbH-UFZ Aptamers that are specific for immunoglobulin-binding cell wall proteins
DE102011006612B3 (de) 2011-03-31 2012-04-12 Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz Aminoglykosid-spezifische Aptamere
DE102011006610B3 (de) 2011-03-31 2012-06-21 Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz Aptamere, die spezifisch sind für Immunoglobulin bindende Zellwandproteine
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
WO2012161677A1 (en) 2011-05-20 2012-11-29 Alcon Research, Ltd. TRANSFERRIN/TRANSFERRIN RECEPTOR-MEDIATED siRNA DELIVERY
EP2723897A4 (de) * 2011-06-24 2015-03-18 Nanostring Technologies Inc Multivariate diagnostische tests und verfahren zu ihrer verwendung
CN102253194B (zh) * 2011-06-25 2013-08-28 南方医科大学 一种采用安培免疫传感器进行夹心免疫反应的二抗探针
US8691500B2 (en) 2011-07-29 2014-04-08 Korea Institute Of Science And Technology Device and method for detecting biomolecule
KR101292636B1 (ko) * 2011-08-04 2013-08-02 한국식품연구원 식중독균 검출 센서용 슬라이드 칩 및 그 제조방법
WO2013046033A1 (en) * 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
EP3252173A1 (de) 2011-10-21 2017-12-06 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren
CA2864035C (en) * 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
US10598650B2 (en) 2012-08-22 2020-03-24 General Electric Company System and method for measuring an operative condition of a machine
DE112013004129T5 (de) 2012-08-22 2015-05-21 General Electric Company Drahtloses System und Verfahren zum Messen einer Betriebsbedingung einer Maschine
US10684268B2 (en) 2012-09-28 2020-06-16 Bl Technologies, Inc. Sensor systems for measuring an interface level in a multi-phase fluid composition
US9982292B2 (en) 2012-09-28 2018-05-29 Src, Inc. Detecting chemical and biological agents using textile-based sensors
JP6395718B2 (ja) * 2012-11-19 2018-09-26 アプトン バイオシステムズ インコーポレイテッド 単一分子検出を用いた分子分析物のデジタル分析の方法
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
WO2014113740A1 (en) 2013-01-18 2014-07-24 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Removal of carbon dioxide via dialysis
US10023869B2 (en) 2013-02-05 2018-07-17 Auramer Bio Limited Bio-sensor for the detection of small molecules
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
DE102013204057A1 (de) 2013-03-08 2014-09-11 Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz Fluorchinolon-spezifische Aptamere
US12016682B2 (en) * 2013-03-14 2024-06-25 The Regents Of The University Of California In-vivo monitoring of molecular targets
WO2014143637A1 (en) 2013-03-15 2014-09-18 The Board Of Trustees Of The University Of Illinois Methods and compositions for enhancing immunoassays
CA2921809A1 (en) * 2013-08-22 2015-02-26 Apton Biosystems, Inc. Digital analysis of molecular analytes using electrical methods
US9874554B1 (en) * 2014-07-16 2018-01-23 Verily Life Sciences Llc Aptamer-based in vivo diagnostic system
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
CN104569420B (zh) * 2014-10-09 2016-08-17 南京大学 适配体修饰的纳米银探针及其应用
WO2016059436A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Method for nanopore rna characterisation
CN104297309B (zh) * 2014-10-29 2016-08-17 江苏出入境检验检疫局动植物与食品检测中心 检测致病菌的电化学传感器、制备方法及其应用
US20180036427A1 (en) 2015-03-04 2018-02-08 Inserm (Institut National De La Santé Etde La Rech Médicale) Methods and pharmaceutical compositions for the treatment of hiv infection
AU2016270823B2 (en) 2015-06-01 2020-09-03 California Institute Of Technology Compositions and methods for screening T cells with antigens for specific populations
EP3380841B1 (de) 2015-11-27 2019-10-02 AIT Austrian Institute of Technology GmbH Autonome abtastmoleküle (asm)
US20190002882A1 (en) 2015-11-27 2019-01-03 Ait Austrian Institute Of Technology Gmbh Molecular robot
KR101928620B1 (ko) * 2016-07-01 2018-12-12 중앙대학교 산학협력단 표적 단백질 검출을 위한 압타머-면역세포 바이오프로브
US20190276828A1 (en) * 2016-10-25 2019-09-12 Purdue Research Foundation Conjugates for sensing
TW201831694A (zh) * 2016-11-08 2018-09-01 美商Ｄｏｔｓ科技公司 過敏原偵測劑和檢定
CN107058501A (zh) * 2017-02-15 2017-08-18 济南大学 一种基于核酸适配体检测氨苄青霉素的方法
EP3592768B1 (de) 2017-03-08 2024-12-25 The Regents of The University of Michigan Analytnachweis
EP3592862A1 (de) 2017-03-08 2020-01-15 California Institute of Technology Paarung der antigenspezifität einer t-zelle mit t-zell-rezeptor-sequenzen
EP3595806A4 (de) 2017-03-17 2021-06-16 Apton Biosystems, Inc. Sequenzierung und hochauflösende bildgebung
CN109001165B (zh) * 2017-06-07 2020-11-20 天津师范大学 一种免标记磷光探针定量检测三磷酸腺苷的方法
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
GB201720162D0 (en) * 2017-12-04 2018-01-17 Univ Oxford Innovation Ltd Method
WO2019200326A1 (en) 2018-04-13 2019-10-17 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
WO2020061237A1 (en) 2018-09-19 2020-03-26 Apton Biosystems, Inc. Densely-packed analyte layers and detection methods
WO2020156029A1 (zh) * 2019-01-30 2020-08-06 苏州宇测生物科技有限公司 一种单分子定量检测方法及检测系统
CN110082524B (zh) * 2019-04-29 2022-06-14 上海大学 检测脂多糖用荧光传感器、其制备方法和应用
GB201907007D0 (en) * 2019-05-17 2019-07-03 Nano Biosols Ltd A simple method and kit for quantifying gold nanoparticles in a liquid sample
WO2021034995A1 (en) * 2019-08-20 2021-02-25 Dots Technology Corp. Optimized nucleic acid probes for analyte detection
US11624606B2 (en) * 2020-02-20 2023-04-11 Cognex Corporation Methods and apparatus for using range data to predict object features
WO2022060728A1 (en) * 2020-09-15 2022-03-24 Palamedrix, Inc. Structure and methods for detection of sample analytes
CN112326602B (zh) * 2020-10-15 2023-07-21 哈尔滨工业大学 一种可实现肿瘤光学诊断与光热治疗双重功能的纳米复合物的筛选方法及其应用
EP4373970A4 (de) * 2021-07-23 2025-07-23 Univ Michigan Regents Entschützungszählsonden zum nachweis und zur quantifizierung von einzelnen molekularen analyten
US11530406B1 (en) 2021-08-30 2022-12-20 Sachi Bioworks Inc. System and method for producing a therapeutic oligomer
US12636303B2 (en) 2021-08-30 2026-05-26 Sachi Bioworks Inc. Composition of a therapeutic oligomer
WO2024191806A1 (en) * 2023-03-10 2024-09-19 Illumina, Inc. Aptamer detection techniques
GB2639519A (en) * 2023-10-26 2025-10-01 Todd Daniel Nanites: smart, programmable polymer composite nucleic-acid nanostructures for sensitive, targeted, molecular nucleic acid detection
WO2026011085A1 (en) * 2024-07-03 2026-01-08 Incyto Discovery Llc Nanoparticle compositions and methods for biological measurements

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193983A (en) * 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
NL7807532A (nl) * 1978-07-13 1980-01-15 Akzo Nv Metaal-immunotest.
US4318707A (en) * 1978-11-24 1982-03-09 Syva Company Macromolecular fluorescent quencher particle in specific receptor assays
US4256834A (en) * 1979-04-09 1981-03-17 Syva Company Fluorescent scavenger particle immunoassay
US4261968A (en) * 1979-05-10 1981-04-14 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4376110A (en) * 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US4650770A (en) * 1981-04-27 1987-03-17 Syntex (U.S.A.) Inc. Energy absorbing particle quenching in light emitting competitive protein binding assays
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
US4713348A (en) * 1983-04-05 1987-12-15 Syntex (U.S.A.) Inc. Fluorescent multiparameter particle analysis
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4851337A (en) * 1986-01-08 1989-07-25 Hygeia Sciences, Inc. Extraction of test substances
US5137827A (en) * 1986-03-25 1992-08-11 Midwest Research Technologies, Inc. Diagnostic element for electrical detection of a binding reaction
US5514602A (en) * 1986-06-09 1996-05-07 Ortho Diagnostic Systems, Inc. Method of producing a metal sol reagent containing colloidal metal particles
US5360895A (en) * 1987-04-22 1994-11-01 Associated Universities, Inc. Derivatized gold clusters and antibody-gold cluster conjugates
US4853335A (en) * 1987-09-28 1989-08-01 Olsen Duane A Colloidal gold particle concentration immunoassay
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5252459A (en) * 1988-09-23 1993-10-12 Abbott Laboratories Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles
US4966143A (en) * 1988-12-06 1990-10-30 Meinershagen Charles I Surgical wire guide
US5151510A (en) * 1990-04-20 1992-09-29 Applied Biosystems, Inc. Method of synethesizing sulfurized oligonucleotide analogs
US5789163A (en) * 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
ATE318832T1 (de) * 1990-06-11 2006-03-15 Gilead Sciences Inc Verfahren zur vervendung von nukleinsäureliganden
US5567588A (en) * 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5460831A (en) * 1990-06-22 1995-10-24 The Regents Of The University Of California Targeted transfection nanoparticles
US5665582A (en) * 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ　プラント　ジェネティクス 磁気性粒子を使用する生物学的材料の単離
US5294369A (en) * 1990-12-05 1994-03-15 Akzo N.V. Ligand gold bonding
US5840867A (en) * 1991-02-21 1998-11-24 Gilead Sciences, Inc. Aptamer analogs specific for biomolecules
EP0613585A4 (de) * 1991-11-22 1995-06-21 Univ California Halbleitende nanokristalle, die durch selbstzusammengesetzte einzelschichten mit festen anorganischen oberflüchen verbunden sind.
US5225064A (en) * 1992-01-15 1993-07-06 Enzyme Technology Research Group, Inc. Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
US5472881A (en) * 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
JPH08507203A (ja) * 1992-12-04 1996-08-06 イノーバー ラボラトリーズ，インコーポレイテッド 調節可能な核酸治療およびそれらの使用方法
US5637508A (en) * 1993-03-26 1997-06-10 Geo-Centers, Inc. Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same
US5384265A (en) * 1993-03-26 1995-01-24 Geo-Centers, Inc. Biomolecules bound to catalytic inorganic particles, immunoassays using the same
US5681943A (en) * 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
US5543158A (en) * 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
US5466580A (en) * 1993-09-22 1995-11-14 Xoma Corporation Method for quantifying BPI in body fluids
US5521289A (en) * 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
US6025202A (en) * 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
US5609907A (en) * 1995-02-09 1997-03-11 The Penn State Research Foundation Self-assembled metal colloid monolayers
IL126544A (en) * 1996-04-25 2004-08-31 Genicon Sciences Inc Analyte assay using scattered-light detectable particles
WO1998004740A1 (en) * 1996-07-29 1998-02-05 Nanosphere Llc Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) * 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US5830986A (en) * 1996-10-28 1998-11-03 Massachusetts Institute Of Technology Methods for the synthesis of functionalizable poly(ethylene oxide) star macromolecules
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US5922537A (en) * 1996-11-08 1999-07-13 N.o slashed.AB Immunoassay, Inc. Nanoparticles biosensor
US5939021A (en) * 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay
US6001570A (en) * 1997-02-18 1999-12-14 Invitro Diagnostics, Inc. Compositions, methods, kits and apparatus for determining the presence or absence of target molecules
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6149868A (en) * 1997-10-28 2000-11-21 The Penn State Research Foundation Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches
US5990479A (en) * 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US6225058B1 (en) * 1998-01-13 2001-05-01 Invitro Diagnostics, Inc. Compositions, methods, kits and apparatus for determining the presence or absence of target molecules
US5972615A (en) * 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US6406921B1 (en) * 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6399302B1 (en) * 1998-08-21 2002-06-04 University Of Virginia Patent Foundation Signal generating oligonucleotide-based biosensor
US6306610B1 (en) * 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6251303B1 (en) * 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6203989B1 (en) * 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
FI982489L (fi) * 1998-11-17 2000-05-18 Arto Remes Menetelmä lihasten harjoittamiseksi
US6277489B1 (en) * 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
ES2459745T3 (es) * 1999-05-14 2014-05-12 Brandeis University Detección basada en aptámero
US6680377B1 (en) * 1999-05-14 2004-01-20 Brandeis University Nucleic acid-based detection
ATE502117T1 (de) * 1999-06-25 2011-04-15 Nanosphere Inc Nanopartikel mit gebundenen oligonukleotiden und deren verwendungen
CA2379711A1 (en) * 1999-07-02 2001-01-25 Conceptual Mindworks, Inc Organic semiconductor recognition complex and system
CA2396113C (en) * 2000-01-13 2009-04-07 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU2001257091A1 (en) * 2000-04-18 2001-10-30 Gilead Sciences, Inc. Aptamer based two-site binding assay
US20020001810A1 (en) * 2000-06-05 2002-01-03 Farrell Michael Patrick Q-beta replicase based assays; the use of chimeric DNA-RNA molecules as probes from which efficient Q-beta replicase templates can be generated in a reverse transcriptase dependent manner
AU2001276870B2 (en) * 2000-07-11 2006-07-20 Northwestern University Method of detection by enhancement of silver staining
US6649138B2 (en) * 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
WO2002046483A2 (en) * 2000-12-06 2002-06-13 Northwestern University Silver stain removal from dna detection chips by cyanide etching or sonication
US20020127563A1 (en) * 2001-01-08 2002-09-12 Salafsky Joshua S. Method and apparatus using a surface-selective nonlinear optical technique for detection of probe-target interactions without labels
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
PT2070939E (pt) * 2001-05-25 2014-06-09 Univ Duke Moduladores de agentes farmacológicos
US7147687B2 (en) * 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
AU2002239726A1 (en) * 2001-05-25 2002-12-09 Northwestern University Non-alloying core shell nanoparticles
ATE423975T1 (de) * 2001-08-03 2009-03-15 Nanosphere Inc Nanopartikelabbildungssystem und -verfahren
ATE437216T1 (de) * 2001-09-06 2009-08-15 Genomic Profiling Systems Inc Schnellnachweis replizierender zellen
CA2466656C (en) * 2001-11-09 2013-07-02 Nanosphere, Inc. Bioconjugate-nanoparticle probes
US20030162190A1 (en) * 2001-11-15 2003-08-28 Gorenstein David G. Phosphoromonothioate and phosphorodithioate oligonucleotide aptamer chip for functional proteomics
CA2473683A1 (en) * 2002-01-22 2003-07-31 Mcmaster University Signalling aptamer complexes
DE60325564D1 (de) * 2002-03-05 2009-02-12 Univ Texas Biospezifische kontrastmittel
US20030211488A1 (en) * 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
DE10228260A1 (de) * 2002-06-25 2004-01-22 Bayer Ag Methode und Vorrichtung zum impedimetrischen Nachweis eines oder mehrerer Analyten in einer Probe
JP2005532456A (ja) * 2002-07-02 2005-10-27 ナノスフェアー　インコーポレイテッド ナノ粒子ポリアニオン複合物ならびに分析物の検出における同複合物の用途
WO2004013628A1 (en) * 2002-08-02 2004-02-12 Applera Corporation Fluorescence polarization assay
JP2005537487A (ja) * 2002-09-03 2005-12-08 ツェプトゼンス　アクチエンゲゼルシャフト 試料中において任意には分画された後の試料中において、固定化特異的結合パートナーとして測定される分析対象物を用いる分析プラットフォーム及び検出法
ATE441861T1 (de) * 2003-05-30 2009-09-15 Nanosphere Inc Verfahren zum nachweis von analyten auf grundlage von evaneszenzbelichtung und auf streuung beruhender nachweis von nanopartikelsondenkomplexen

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