ATE428002T1 - Nachweis von immobilisierter nukleinsaure - Google Patents

Nachweis von immobilisierter nukleinsaure

Info

Publication number
ATE428002T1
ATE428002T1 AT04789513T AT04789513T ATE428002T1 AT E428002 T1 ATE428002 T1 AT E428002T1 AT 04789513 T AT04789513 T AT 04789513T AT 04789513 T AT04789513 T AT 04789513T AT E428002 T1 ATE428002 T1 AT E428002T1
Authority
AT
Austria
Prior art keywords
nucleic acid
immobilized nucleic
methods
dye compound
solid
Prior art date
Application number
AT04789513T
Other languages
English (en)
Inventor
Matthew Beaudet
Gregory Cox
Stephen Yue
Original Assignee
Molecular Probes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Probes Inc filed Critical Molecular Probes Inc
Application granted granted Critical
Publication of ATE428002T1 publication Critical patent/ATE428002T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT04789513T 2003-09-30 2004-09-30 Nachweis von immobilisierter nukleinsaure ATE428002T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50763003P 2003-09-30 2003-09-30

Publications (1)

Publication Number Publication Date
ATE428002T1 true ATE428002T1 (de) 2009-04-15

Family

ID=34421643

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04789513T ATE428002T1 (de) 2003-09-30 2004-09-30 Nachweis von immobilisierter nukleinsaure

Country Status (7)

Country Link
US (7) US7727716B2 (de)
EP (2) EP1668162B1 (de)
JP (2) JP4651621B2 (de)
AT (1) ATE428002T1 (de)
CA (1) CA2540508A1 (de)
DE (1) DE602004020484D1 (de)
WO (1) WO2005033342A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4651621B2 (ja) 2003-09-30 2011-03-16 モレキュラー プローブス， インコーポレイテッド 固定化された核酸の検出
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
EP2062036A4 (de) * 2006-08-31 2016-09-21 Life Technologies Israel Ltd Verfahren, kassetten, gele und vorrichtungen zur isolation und sammlung von biomolekülen aus elektrophorese-gelen
CN101535412B (zh) * 2006-11-02 2014-05-07 霍夫曼-拉罗奇有限公司 新型双链dna结合荧光染料
EP3118201A1 (de) * 2007-10-02 2017-01-18 Thermo Fisher Scientific Inc. Monomethin-farbstoffe
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
EP2697730A4 (de) 2011-04-15 2015-04-15 Advanced Diamond Technologies Inc Elektrochemisches system und verfahren zur vor-ort-erzeugung von oxidationsmitteln bei hoher stromdichte
JP2013040136A (ja) * 2011-08-17 2013-02-28 Tosoh Corp ４−アルコキシ−２−（４−アミノスチリル）ベンゾチアゾリウム塩、その製造方法およびそれを用いる核酸の検出方法
US20160010145A1 (en) * 2012-12-07 2016-01-14 Life Technologies Corporation Detection of rna with substituted unsymmetrical cyanine dyes
US10011570B2 (en) 2013-10-03 2018-07-03 Biotium, Inc. Nucleic acid binding dyes with improved safety
CN104849253B (zh) * 2014-06-11 2017-07-18 南方医科大学 苯并吲哚半菁染料检测葡萄糖的用途
US9969887B2 (en) * 2016-04-20 2018-05-15 Sfc Co., Ltd. Merocyanine-based compounds, and dyes, kits and contrast medium compositions for labelling biomolecules comprising the same
US20200232944A1 (en) * 2017-09-30 2020-07-23 Bioatlas Oü Multicomponent tablet for making agarose gel and methods thereof
CN114174799A (zh) * 2019-05-30 2022-03-11 贝克曼库尔特有限公司 用于固定生物标本以进行显微成像的方法和系统
US12169166B2 (en) 2021-02-16 2024-12-17 Aat Bioquest, Inc. Fluorogenic cyanine compounds for detecting nucleic acids
CN119301272A (zh) * 2022-06-02 2025-01-10 瑞孚迪健康科学公司 腺相关病毒衣壳的dna含量的电泳介导的表征
US20240191297A1 (en) 2022-10-14 2024-06-13 10X Genomics, Inc. Methods, compositions, and systems for assessing biological sample quality
CN122374014A (zh) * 2023-12-11 2026-07-10 库贝生物科技有限公司 包含功能化琼脂糖颗粒的片剂、其生产方法以及该片剂的用途

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Publication number Priority date Publication date Assignee Title
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4957870A (en) 1985-11-01 1990-09-18 Becton, Dickinson And Company Detection of Reticulocytes, RNA and DNA
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5534416A (en) 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5656449A (en) * 1995-03-06 1997-08-12 Molecular Probes, Inc. Neutral unsymmetrical cyanine dyes
US5582702A (en) 1995-04-26 1996-12-10 Ethrog Biotechnology Ltd. Apparatus and method for electrophoresis
US6379516B1 (en) * 1995-04-26 2002-04-30 Ethrog Biotechnology Ltd. Apparatus and method for electrophoresis
US7824532B2 (en) 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
US5929227A (en) 1995-07-12 1999-07-27 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
EP0965034B1 (de) 1997-03-07 2007-05-30 Clare Chemical Research, Inc. Fluorometrischer Nachweis mit sichtbarem Light
US5865874A (en) * 1997-06-27 1999-02-02 Duracell Inc. Hydrogen storage alloy
CA2321100A1 (en) 1998-02-19 1999-08-26 Edvotek Articles of manufacture and methods for staining biomolecules
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
IL157813A0 (en) 2001-03-08 2004-03-28 Ethrog Biotechnology Ltd Apparatus and method for electrophoresis
JP4651621B2 (ja) * 2003-09-30 2011-03-16 モレキュラー プローブス， インコーポレイテッド 固定化された核酸の検出

Also Published As

Publication number Publication date
US20140076726A1 (en) 2014-03-20
DE602004020484D1 (de) 2009-05-20
US7727716B2 (en) 2010-06-01
CA2540508A1 (en) 2005-04-14
EP1668162B1 (de) 2009-04-08
WO2005033342A1 (en) 2005-04-14
US20150247799A1 (en) 2015-09-03
JP2007507238A (ja) 2007-03-29
US20100216665A1 (en) 2010-08-26
US20130011927A1 (en) 2013-01-10
US20100227331A1 (en) 2010-09-09
US7977057B2 (en) 2011-07-12
US8969004B2 (en) 2015-03-03
EP1668162A1 (de) 2006-06-14
JP4651621B2 (ja) 2011-03-16
US20120058566A1 (en) 2012-03-08
US20050239096A1 (en) 2005-10-27
JP2011099862A (ja) 2011-05-19
EP1988177A1 (de) 2008-11-05

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