ATE428770T1 - Verstärkte reaktivierung wärmebeständiger, reversibel inaktivierter enzyme - Google Patents

Verstärkte reaktivierung wärmebeständiger, reversibel inaktivierter enzyme

Info

Publication number
ATE428770T1
ATE428770T1 AT07111041T AT07111041T ATE428770T1 AT E428770 T1 ATE428770 T1 AT E428770T1 AT 07111041 T AT07111041 T AT 07111041T AT 07111041 T AT07111041 T AT 07111041T AT E428770 T1 ATE428770 T1 AT E428770T1
Authority
AT
Austria
Prior art keywords
resistant
heat
reversibly inactivated
inactivated enzymes
reactivation
Prior art date
Application number
AT07111041T
Other languages
English (en)
Inventor
Dirk Loeffert
Ralf Peist
Patrick Baumhof
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE428770T1 publication Critical patent/ATE428770T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/99—Enzyme inactivation by chemical treatment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AT07111041T 2006-06-28 2007-06-26 Verstärkte reaktivierung wärmebeständiger, reversibel inaktivierter enzyme ATE428770T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81704306P 2006-06-28 2006-06-28
US85280406P 2006-10-19 2006-10-19

Publications (1)

Publication Number Publication Date
ATE428770T1 true ATE428770T1 (de) 2009-05-15

Family

ID=38581995

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07111041T ATE428770T1 (de) 2006-06-28 2007-06-26 Verstärkte reaktivierung wärmebeständiger, reversibel inaktivierter enzyme

Country Status (6)

Country Link
US (2) US8435774B2 (de)
EP (1) EP1873238B1 (de)
JP (1) JP5219409B2 (de)
CN (1) CN101100662B (de)
AT (1) ATE428770T1 (de)
DE (1) DE602007000894D1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8435774B2 (en) 2006-06-28 2013-05-07 Qiagen Gmbh Enhancing reactivation of thermostable reversibly inactivated enzymes
EP2560488B1 (de) 2010-04-23 2015-10-28 Cytokinetics, Inc. Bestimmte aminopyrimidine und aminotriazine, zusammensetzungen daraus und verfahren zu ihrer verwendung
AR081626A1 (es) 2010-04-23 2012-10-10 Cytokinetics Inc Compuestos amino-piridazinicos, composiciones farmaceuticas que los contienen y uso de los mismos para tratar trastornos musculares cardiacos y esqueleticos
AR081331A1 (es) 2010-04-23 2012-08-08 Cytokinetics Inc Amino- pirimidinas composiciones de las mismas y metodos para el uso de los mismos
CN102958927A (zh) 2010-05-12 2013-03-06 Abbvie公司 激酶的吲唑抑制剂
AP2013006706A0 (en) 2010-07-02 2013-02-28 Gilead Sciences Inc Napht-2-ylacetic acid derivatives to treat AIDS
PH12013500015A1 (en) 2010-07-02 2013-02-18 Gilead Sciences Inc 2 -quinolinyl- acetic acid derivatives as hiv antiviral compounds
UA111841C2 (uk) 2011-04-21 2016-06-24 Гіліад Сайєнсіз, Інк. Сполуки бензотіазолу та їх фармацевтичне застосування
US8759380B2 (en) 2011-04-22 2014-06-24 Cytokinetics, Inc. Certain heterocycles, compositions thereof, and methods for their use
US9376392B2 (en) 2012-01-04 2016-06-28 Gilead Sciences, Inc. 2-(tert-butoxy)-2-(7-methylquinolin-6-yl) acetic acid derivatives for treating AIDS
WO2013103738A1 (en) 2012-01-04 2013-07-11 Gilead Sciences, Inc. Napthalene acetic acid derivatives against hiv infection
US9096586B2 (en) 2012-04-20 2015-08-04 Gilead Sciences, Inc. Therapeutic compounds
ES2846833T3 (es) 2016-07-18 2021-07-29 Janssen Pharmaceutica Nv Ligandos de obtención de imágenes de tau por PET
GB201813429D0 (en) * 2018-08-17 2018-10-03 Touchlight Ip Ltd Synthesis of DNA with improved yield

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9509336D0 (en) * 1995-05-09 1995-06-28 Dynal As Chemical method
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
JP3968606B2 (ja) * 1997-09-03 2007-08-29 東洋紡績株式会社 超好熱始原菌Ｐｙｒｏｃｏｃｃｕｓ ｓｐ．ＫＯＤ１株由来ＤＮＡポリメラーゼに特異的なモノクローナル抗体を利用した核酸増幅法
US6183998B1 (en) * 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
JP2002199877A (ja) * 2000-11-30 2002-07-16 Advanced Biotechnologies Ltd 酵素の可逆的な不活性化およびその酵素を含むキット
US20020184551A1 (en) * 2001-03-28 2002-12-05 Council Of Scientific & Industrial Research Process for the preparation of deactivated rice bran lipase
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US20060019366A1 (en) * 2004-06-09 2006-01-26 Wanli Bi Reversibly modified thermostable enzyme compositions and methods of making and using the same
US8435774B2 (en) 2006-06-28 2013-05-07 Qiagen Gmbh Enhancing reactivation of thermostable reversibly inactivated enzymes

Also Published As

Publication number Publication date
JP5219409B2 (ja) 2013-06-26
EP1873238A1 (de) 2008-01-02
DE602007000894D1 (de) 2009-05-28
US8435774B2 (en) 2013-05-07
JP2008005841A (ja) 2008-01-17
CN101100662A (zh) 2008-01-09
US20080003656A1 (en) 2008-01-03
EP1873238B1 (de) 2009-04-15
US8728786B2 (en) 2014-05-20
CN101100662B (zh) 2011-07-13
US20130217092A1 (en) 2013-08-22

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