ATE432347T1 - Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen - Google Patents
Methoden und systeme zur detektion und isolation von nucleinsäuresequenzenInfo
- Publication number
- ATE432347T1 ATE432347T1 AT03780549T AT03780549T ATE432347T1 AT E432347 T1 ATE432347 T1 AT E432347T1 AT 03780549 T AT03780549 T AT 03780549T AT 03780549 T AT03780549 T AT 03780549T AT E432347 T1 ATE432347 T1 AT E432347T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- isolation
- molecules
- rna
- nucleotide sequence
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 150000007523 nucleic acids Chemical group 0.000 title abstract 4
- 238000002955 isolation Methods 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title 1
- 108020004999 messenger RNA Proteins 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 238000001574 biopsy Methods 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 241001493065 dsRNA viruses Species 0.000 abstract 1
- 229920001519 homopolymer Polymers 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 241001430294 unidentified retrovirus Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39092802P | 2002-06-24 | 2002-06-24 | |
| PCT/IB2003/006354 WO2004020575A2 (en) | 2002-06-24 | 2003-06-20 | Methods and systems for detection and isolation of a nucleotide sequence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE432347T1 true ATE432347T1 (de) | 2009-06-15 |
Family
ID=31978221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03780549T ATE432347T1 (de) | 2002-06-24 | 2003-06-20 | Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20050053942A1 (de) |
| EP (1) | EP1527175B1 (de) |
| AT (1) | ATE432347T1 (de) |
| AU (1) | AU2003288474A1 (de) |
| DE (1) | DE60327775D1 (de) |
| WO (1) | WO2004020575A2 (de) |
Families Citing this family (50)
| Publication number | Priority date | Publication date | Assignee | Title |
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| DE102005011350A1 (de) * | 2005-03-11 | 2006-09-14 | Eppendorf Ag | CDNA Microarrays mit Zufallsspacern |
| US20070072223A1 (en) * | 2005-09-16 | 2007-03-29 | Primera Biosystems, Inc. | Compositions and methods for purifying nucleic acids |
| US20070212704A1 (en) | 2005-10-03 | 2007-09-13 | Applera Corporation | Compositions, methods, and kits for amplifying nucleic acids |
| JP2007105041A (ja) * | 2005-10-13 | 2007-04-26 | F Hoffmann La Roche Ag | 多重固相核酸増幅アッセイ |
| EP3617321B1 (de) * | 2006-05-31 | 2024-10-23 | Sequenom, Inc. | Kit zur nukleinsäureextraktion und -verstärkung aus einer probe |
| CN102016035A (zh) | 2006-11-02 | 2011-04-13 | 犹他大学研究基金会 | 可用于等位基因特异pcr的寡聚核苷酸 |
| WO2008150937A2 (en) * | 2007-05-29 | 2008-12-11 | Aivaradx, Inc. | Real-time pcr |
| DK2191016T3 (en) * | 2007-08-20 | 2015-06-08 | Gen Hospital Corp | INSULATION OF PROTEIN FACTORS BINDING DIRECT OR INDIRECT WITH NUCLEIC ACIDS |
| EP2195428B1 (de) | 2007-09-19 | 2013-12-11 | Applied Biosystems, LLC | Sirna-sequenz-unabhängige modifikationsformate zur verringerung von das ziel verfehlenden phänotypischen effekten bei rnai und stabilisierte formen davon |
| EP3514244B1 (de) | 2009-04-03 | 2021-07-07 | Sequenom, Inc. | Verfahren für die herstellung von nukleinsäuren |
| WO2011035135A1 (en) * | 2009-09-18 | 2011-03-24 | The Trustees Of The University Of Pennsylvania | Novel molecular beacons |
| US8278049B2 (en) * | 2010-04-26 | 2012-10-02 | Ann & Robert H. Lurie Children's Hospital of Chicago | Selective enrichment of CpG islands |
| WO2012006551A2 (en) | 2010-07-08 | 2012-01-12 | The Brigham And Women's Hospital, Inc. | Neuroprotective molecules and methods of treating neurological disorders and inducing stress granules |
| AU2012236099A1 (en) | 2011-03-31 | 2013-10-03 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
| US20140329700A1 (en) * | 2011-08-23 | 2014-11-06 | Rutgers, The State University Of New Jersey | Methods of isolating rna and mapping of polyadenylation isoforms |
| CN104204224B (zh) | 2011-11-23 | 2018-04-24 | 康奈尔大学 | 用于诊断人免疫缺陷病毒感染的方法 |
| US9725770B2 (en) * | 2012-03-06 | 2017-08-08 | The Regents Of The University Of California | Methods and compositions for identification of source of microbial contamination in a sample |
| WO2013151665A2 (en) | 2012-04-02 | 2013-10-10 | modeRNA Therapeutics | Modified polynucleotides for the production of proteins associated with human disease |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| WO2014134144A1 (en) | 2013-02-28 | 2014-09-04 | The General Hospital Corporation | Mirna profiling compositions and methods of use |
| ES2708562T3 (es) | 2013-03-14 | 2019-04-10 | Translate Bio Inc | Evaluación cuantitativa de la eficacidad de tapa de ARN mensajero |
| BR112015022505A2 (pt) | 2013-03-14 | 2017-10-24 | Shire Human Genetic Therapies | avaliação quantitativa para eficiência do cap de rna mensageiro |
| WO2014152031A1 (en) * | 2013-03-15 | 2014-09-25 | Moderna Therapeutics, Inc. | Ribonucleic acid purification |
| EP2971165A4 (de) | 2013-03-15 | 2016-11-23 | Moderna Therapeutics Inc | Entfernung von dna-fragmenten in mrna-herstellungsverfahren |
| US10590161B2 (en) | 2013-03-15 | 2020-03-17 | Modernatx, Inc. | Ion exchange purification of mRNA |
| WO2014152027A1 (en) | 2013-03-15 | 2014-09-25 | Moderna Therapeutics, Inc. | Manufacturing methods for production of rna transcripts |
| CN103308373A (zh) * | 2013-06-05 | 2013-09-18 | 南昌大学 | 磁珠分离大肠杆菌o157的方法 |
| CA2917348A1 (en) | 2013-07-11 | 2015-01-15 | Moderna Therapeutics, Inc. | Compositions comprising synthetic polynucleotides encoding crispr related proteins and synthetic sgrnas and methods of use |
| EP3052511A4 (de) | 2013-10-02 | 2017-05-31 | Moderna Therapeutics, Inc. | Polynukleotidmoleküle und verwendungen davon |
| WO2015196128A2 (en) | 2014-06-19 | 2015-12-23 | Moderna Therapeutics, Inc. | Alternative nucleic acid molecules and uses thereof |
| US10407683B2 (en) | 2014-07-16 | 2019-09-10 | Modernatx, Inc. | Circular polynucleotides |
| US10093967B2 (en) * | 2014-08-12 | 2018-10-09 | The Regents Of The University Of Michigan | Detection of nucleic acids |
| WO2016094839A2 (en) | 2014-12-12 | 2016-06-16 | Exact Sciences Corporation | Compositions and methods for performing methylation detection assays |
| CA3183545C (en) * | 2014-12-12 | 2026-01-13 | Exact Sciences Corporation | Compositions and methods for performing methylation detection assays |
| EP3350333B2 (de) | 2015-09-17 | 2025-08-06 | ModernaTX, Inc. | Polynukleotide mit stabilisierendem schwanzbereich |
| WO2017049286A1 (en) | 2015-09-17 | 2017-03-23 | Moderna Therapeutics, Inc. | Polynucleotides containing a morpholino linker |
| EP4299763A2 (de) * | 2015-09-22 | 2024-01-03 | Biocartis NV | Verbesserte detektion von kurzen homopolymeren wiederholungen |
| WO2017068087A1 (en) * | 2015-10-22 | 2017-04-27 | Roche Innovation Center Copenhagen A/S | Oligonucleotide detection method |
| WO2017223176A1 (en) | 2016-06-24 | 2017-12-28 | Modernatx, Inc. | Methods and apparatus for filtration |
| WO2018165309A1 (en) | 2017-03-08 | 2018-09-13 | The Regents Of The University Of Michigan | Analyte detection |
| CN108519481B (zh) * | 2018-03-08 | 2020-10-16 | 捷和泰（北京）生物科技有限公司 | 一种改善核心抗体磁微粒化学发光免疫分析精密度的方法 |
| JP7347849B2 (ja) | 2018-04-19 | 2023-09-20 | ファースト ライト ダイアグノスティクス， インコーポレイテッド | 標的の検出 |
| CA3111562A1 (en) | 2018-09-07 | 2020-04-02 | The General Hospital Corporation | Compositions and methods for immune checkpoint inhibition |
| JP7659314B2 (ja) * | 2018-10-04 | 2025-04-09 | ファースト ライト ダイアグノスティックス， インコーポレイテッド | 分析機器 |
| EP3999624B1 (de) | 2019-07-16 | 2026-03-25 | Meliolabs Inc. | Verfahren und vorrichtungen für einzellenbasierte digitale hochauflösende schmelzanalyse |
| US20220325322A1 (en) * | 2019-08-02 | 2022-10-13 | Vladimir I. Slepnev | Sequence specific nucleic acid enrichment methods and uses thereof |
| CA3178629A1 (en) * | 2020-05-29 | 2021-12-02 | Jesus Ching | Programmable nuclease diagnostic device |
| AU2021409643A1 (en) * | 2020-12-21 | 2023-01-05 | Illumina, Inc. | Compositions and methods for capturing and amplifying target polynucleotides using modified capture primers |
| CN114107446A (zh) * | 2021-12-16 | 2022-03-01 | 福建和瑞基因科技有限公司 | 一种核酸的检测试剂盒及其检测方法 |
| WO2024015792A2 (en) * | 2022-07-11 | 2024-01-18 | Rensselaer Polytechnic Institute | Ligands and methods of making ligands for affinity capture on a surface of mrna in a solution |
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| US4670379A (en) * | 1984-12-19 | 1987-06-02 | E. I. Du Pont De Nemours And Company | Polynucleotide hydridization assays employing catalyzed luminescence |
| US5310651A (en) * | 1986-01-22 | 1994-05-10 | Institut Pasteur | DNA probes of human immunodeficiency virus type 2 (HIV-2), and methods employing these probes for dectecting the presence of HIV-2 |
| US5622820A (en) * | 1988-03-10 | 1997-04-22 | City Of Hope | Method for amplification and detection of RNA and DNA sequences |
| US5196306A (en) * | 1989-03-29 | 1993-03-23 | E. I. Du Pont De Nemours And Company | Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system |
| US5514545A (en) * | 1992-06-11 | 1996-05-07 | Trustees Of The University Of Pennsylvania | Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics |
| US5804383A (en) * | 1992-08-21 | 1998-09-08 | The Regents Of The University Of California | Method and assay for detection of the expression of allele-specific mutations by allele-specific in situ reverse transcriptase polymerase chain reaction |
| DE4317028C3 (de) * | 1993-05-21 | 2000-06-29 | Martin Busch | Verfahren zur Akquisition und Auswertung von Daten in einem Kernspin-Tomographen |
| US6562596B1 (en) * | 1993-10-06 | 2003-05-13 | Amgen Inc. | Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods |
| US5955072A (en) * | 1994-07-13 | 1999-09-21 | Sankyo Company, Limited | Expression systems utilizing autolyzing fusion proteins and a reducing polypeptide |
| GB9417593D0 (en) * | 1994-09-01 | 1994-10-19 | Secr Defence | Luciferase labelling method |
| US5698686A (en) * | 1994-10-20 | 1997-12-16 | Arch Development Corporation | Yeast telomerase compositions |
| AU2069597A (en) * | 1996-03-04 | 1997-09-22 | Genetrace Systems, Inc. | Methods of screening nucleic acids using mass spectrometry |
| US5713358A (en) * | 1996-03-26 | 1998-02-03 | Wisconsin Alumni Research Foundation | Method for producing a time-resolved series of 3D magnetic resonance angiograms during the first passage of contrast agent |
| US6072043A (en) * | 1996-06-04 | 2000-06-06 | Polyprobe, Inc. | Optimally fluorescent oligonucleotides |
| US5691153A (en) * | 1996-09-06 | 1997-11-25 | Creighton University | Genetic markers to detect high bone mass |
| US5916750A (en) * | 1997-01-08 | 1999-06-29 | Biogenex Laboratories | Multifunctional linking reagents for synthesis of branched oligomers |
| US5873825A (en) * | 1997-04-11 | 1999-02-23 | Wisconsin Alumni Research Foundation | Three dimensional digital subtraction magnetic resonance angiography with limited k-space mask |
| JP4236812B2 (ja) * | 1997-09-12 | 2009-03-11 | エクシコン エ／エス | オリゴヌクレオチド類似体 |
| US6487435B2 (en) * | 1998-04-10 | 2002-11-26 | Wisconsin Alumni Research Foundation | Magnetic resonance angiography using undersampled 3D projection imaging |
| DE60025172T2 (de) * | 1999-03-18 | 2006-09-21 | Exiqon A/S | Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben |
| KR20020013513A (ko) * | 1999-03-18 | 2002-02-20 | 추후제출 | Ｘｙｌｏ-ｌｎａ 유사체 |
| HK1048339A1 (zh) * | 1999-03-18 | 2003-03-28 | 埃克西库恩公司 | 利用特异性lna引物检测基因突变 |
| DE19924448A1 (de) * | 1999-05-28 | 2000-12-07 | Siemens Ag | Verfahren zur Gewinnung von zeit- und ortsaufgelösten dreidimensionalen Datensätzen mittels der magnetischen Resonanz und Vorrichtung zur Durchführung des Verfahrens |
| US20030077609A1 (en) * | 2001-03-25 | 2003-04-24 | Jakobsen Mogens Havsteen | Modified oligonucleotides and uses thereof |
| US7003343B2 (en) * | 2001-03-30 | 2006-02-21 | Cornell Research Foundation, Inc. | Method and apparatus for anatomically tailored k-space sampling and recessed elliptical view ordering for bolus-enhanced 3D MR angiography |
-
2003
- 2003-06-20 AT AT03780549T patent/ATE432347T1/de not_active IP Right Cessation
- 2003-06-20 DE DE60327775T patent/DE60327775D1/de not_active Expired - Lifetime
- 2003-06-20 US US10/601,140 patent/US20050053942A1/en not_active Abandoned
- 2003-06-20 AU AU2003288474A patent/AU2003288474A1/en not_active Abandoned
- 2003-06-20 WO PCT/IB2003/006354 patent/WO2004020575A2/en not_active Ceased
- 2003-06-20 EP EP03780549A patent/EP1527175B1/de not_active Expired - Lifetime
-
2007
- 2007-08-14 US US11/893,108 patent/US20080096191A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1527175A2 (de) | 2005-05-04 |
| AU2003288474A1 (en) | 2004-03-19 |
| US20080096191A1 (en) | 2008-04-24 |
| DE60327775D1 (de) | 2009-07-09 |
| WO2004020575A3 (en) | 2004-12-23 |
| EP1527175B1 (de) | 2009-05-27 |
| US20050053942A1 (en) | 2005-03-10 |
| WO2004020575A2 (en) | 2004-03-11 |
| AU2003288474A8 (en) | 2004-03-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |