ATE432355T1 - Expressionsklonierungsverfahren in filamentösen pilzen - Google Patents

Expressionsklonierungsverfahren in filamentösen pilzen

Info

Publication number
ATE432355T1
ATE432355T1 AT03704338T AT03704338T ATE432355T1 AT E432355 T1 ATE432355 T1 AT E432355T1 AT 03704338 T AT03704338 T AT 03704338T AT 03704338 T AT03704338 T AT 03704338T AT E432355 T1 ATE432355 T1 AT E432355T1
Authority
AT
Austria
Prior art keywords
expression cloning
polypeptide
filamentous fungi
library
cloning process
Prior art date
Application number
AT03704338T
Other languages
English (en)
Inventor
Mary Stringer
Kirk Schnorr
Jesper Vind
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE432355T1 publication Critical patent/ATE432355T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preliminary Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT03704338T 2002-02-19 2003-02-18 Expressionsklonierungsverfahren in filamentösen pilzen ATE432355T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200256 2002-02-19
PCT/DK2003/000106 WO2003070956A1 (en) 2002-02-19 2003-02-18 Expression cloning methods in filamentous fungi

Publications (1)

Publication Number Publication Date
ATE432355T1 true ATE432355T1 (de) 2009-06-15

Family

ID=27741065

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03704338T ATE432355T1 (de) 2002-02-19 2003-02-18 Expressionsklonierungsverfahren in filamentösen pilzen

Country Status (7)

Country Link
US (2) US20050170350A1 (de)
EP (1) EP1478755B1 (de)
AT (1) ATE432355T1 (de)
AU (1) AU2003206683A1 (de)
DE (1) DE60327740D1 (de)
DK (1) DK1478755T3 (de)
WO (1) WO2003070956A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4891220B2 (ja) 2004-02-25 2012-03-07 ノボザイムス アクティーゼルスカブ 菌類細胞壁分解酵素
CN101679993A (zh) 2007-05-09 2010-03-24 诺维信公司 适于选择产生目标多肽的文库克隆的表达克隆方法
ES2547118T3 (es) 2008-02-18 2015-10-01 Meiji Seika Pharma Co., Ltd. Catalasa termoestable
CN102947442B (zh) 2009-11-06 2016-03-09 诺维信股份有限公司 用于糖化纤维素材料的组合物
US9150842B2 (en) 2011-03-09 2015-10-06 Novozymes A/S Methods of increasing the cellulolytic enhancing activity of a polypeptide
US9409958B2 (en) 2011-03-10 2016-08-09 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN103649308A (zh) 2011-04-28 2014-03-19 诺维信股份有限公司 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸
US9624518B2 (en) 2011-04-29 2017-04-18 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
WO2013075644A1 (en) 2011-11-22 2013-05-30 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
RU2619051C2 (ru) 2011-11-25 2017-05-11 Новозимс А/С Полипептиды с лизоцимной активностью и полинуклеотиды, кодирующие их
WO2014086706A1 (en) 2012-12-07 2014-06-12 Novozymes A/S Method for generating site-specific mutations in filamentous fungi
CN110628749A (zh) 2013-03-08 2019-12-31 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
CN105283546B (zh) 2013-05-10 2025-08-12 国投生物科技投资有限公司 具有木聚糖酶活性的多肽以及对其进行编码的多核苷酸
EP3594335B1 (de) 2014-09-05 2024-05-01 Novozymes A/S Kohlenhydratbindende modulvarianten und polynukleotide zur codierung davon
DK3739045T3 (da) 2015-02-24 2024-10-21 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
WO2018085370A1 (en) 2016-11-02 2018-05-11 Novozymes A/S Processes for reducing production of primeverose during enzymatic saccharification of lignocellulosic material
AU2018319349B2 (en) 2017-08-25 2024-02-29 Novozymes A/S Enzyme assisted crude palm oil extraction
CN113302293A (zh) 2018-12-12 2021-08-24 诺维信公司 具有木聚糖酶活性的多肽以及编码这些多肽的多核苷酸
CN114958838B (zh) * 2021-02-23 2025-02-28 中国科学院天津工业生物技术研究所 一组高强度的酿酒酵母人工小启动子
CN114875046B (zh) * 2022-05-30 2023-07-18 福州大学 一种丝状真菌复制子
CN114958899A (zh) * 2022-05-30 2022-08-30 福州大学 一种能在黑曲霉细胞内自主复制的复制子
CN119913118B (zh) * 2025-04-03 2025-06-24 湖南工程学院 一种漆酶突变体及其在处理退浆废水中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0383779B2 (de) * 1987-09-04 2000-05-31 Novo Nordisk A/S VERFAHREN ZUR HERSTELLUNG VON PROTEINPRODUKTEN IN $i(ASPERGILLUS) UND PROMOTOREN ZUR VERWENDUNG IN $i(ASPERGILLUS)
US5648267A (en) * 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
US6107477A (en) * 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
DK1124949T3 (da) * 1998-10-26 2006-11-06 Novozymes As Konstruktion og screening af et DNA-bibliotek af interesse i trådformede svampeceller
AU2001234443A1 (en) * 2000-01-13 2001-07-24 Novozymes Biotech, Inc. Methods for producing a polypeptide using a crippled translational initiator sequence
GB0001401D0 (en) * 2000-01-22 2000-03-08 Univ Edinburgh Methods for amplifying genetic material and use thereof

Also Published As

Publication number Publication date
US20110059862A1 (en) 2011-03-10
AU2003206683A1 (en) 2003-09-09
DK1478755T3 (da) 2009-09-14
DE60327740D1 (de) 2009-07-09
US20050170350A1 (en) 2005-08-04
EP1478755B1 (de) 2009-05-27
EP1478755A1 (de) 2004-11-24
WO2003070956A1 (en) 2003-08-28

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