ATE434043T1 - Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays - Google Patents
Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arraysInfo
- Publication number
- ATE434043T1 ATE434043T1 AT05707166T AT05707166T ATE434043T1 AT E434043 T1 ATE434043 T1 AT E434043T1 AT 05707166 T AT05707166 T AT 05707166T AT 05707166 T AT05707166 T AT 05707166T AT E434043 T1 ATE434043 T1 AT E434043T1
- Authority
- AT
- Austria
- Prior art keywords
- arrays
- dna molecules
- linear dna
- active linear
- interest
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108700026244 Open Reading Frames Proteins 0.000 abstract 1
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 108010026552 Proteome Proteins 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000003491 array Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2005/001078 WO2006081831A1 (en) | 2005-02-03 | 2005-02-03 | Method of generating translationally active linear dna molecules and use thereof in array formats |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE434043T1 true ATE434043T1 (de) | 2009-07-15 |
Family
ID=34960919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05707166T ATE434043T1 (de) | 2005-02-03 | 2005-02-03 | Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20080305961A1 (de) |
| EP (1) | EP1844143B1 (de) |
| AT (1) | ATE434043T1 (de) |
| CA (1) | CA2596758A1 (de) |
| DE (1) | DE602005015028D1 (de) |
| DK (1) | DK1844143T3 (de) |
| WO (1) | WO2006081831A1 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120141577A1 (en) * | 2006-12-04 | 2012-06-07 | Vical Incorporated | Linear expression cassette vaccines |
| WO2009155950A1 (en) | 2008-06-27 | 2009-12-30 | King Faisal Specialist Hospital And Research Centre | Cloning-free method of generating transcriptionally and post-transcriptionally controllable expression active linear reporter constructs |
| GB2483462A (en) | 2010-09-08 | 2012-03-14 | Mologen Ag | Use of a dumbbell-shaped DNA construct for the treatment of cancer through jet-injection administration |
| WO2012147370A1 (ja) * | 2011-04-28 | 2012-11-01 | 国立大学法人山口大学 | ターミネータ配列を含む高発現用リバースプライマー及びリニアｄｎａ |
| EP4010481A4 (de) | 2019-08-09 | 2023-09-20 | Nutcracker Therapeutics, Inc. | Mikrofluidische vorrichtungen und verfahren zur verwendung davon |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5571690A (en) * | 1991-05-08 | 1996-11-05 | The University Of Virginia Patents Foundation | Method for the cell-free synthesis of proteins |
| WO2001020015A1 (en) * | 1999-09-17 | 2001-03-22 | Whitehead Institute For Biomedical Research | Reverse transfection method |
-
2005
- 2005-02-03 AT AT05707166T patent/ATE434043T1/de not_active IP Right Cessation
- 2005-02-03 EP EP05707166A patent/EP1844143B1/de not_active Expired - Lifetime
- 2005-02-03 DK DK05707166T patent/DK1844143T3/da active
- 2005-02-03 WO PCT/EP2005/001078 patent/WO2006081831A1/en not_active Ceased
- 2005-02-03 CA CA002596758A patent/CA2596758A1/en not_active Abandoned
- 2005-02-03 US US11/722,927 patent/US20080305961A1/en not_active Abandoned
- 2005-02-03 DE DE602005015028T patent/DE602005015028D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1844143A1 (de) | 2007-10-17 |
| CA2596758A1 (en) | 2006-08-10 |
| DE602005015028D1 (de) | 2009-07-30 |
| WO2006081831A1 (en) | 2006-08-10 |
| DK1844143T3 (da) | 2009-10-12 |
| EP1844143B1 (de) | 2009-06-17 |
| US20080305961A1 (en) | 2008-12-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |