ATE434051T1 - Hohe expression eines heterologen proteins mit seltenen codons - Google Patents

Hohe expression eines heterologen proteins mit seltenen codons

Info

Publication number
ATE434051T1
ATE434051T1 AT00914444T AT00914444T ATE434051T1 AT E434051 T1 ATE434051 T1 AT E434051T1 AT 00914444 T AT00914444 T AT 00914444T AT 00914444 T AT00914444 T AT 00914444T AT E434051 T1 ATE434051 T1 AT E434051T1
Authority
AT
Austria
Prior art keywords
host cells
expression
heterologue
protein
high expression
Prior art date
Application number
AT00914444T
Other languages
English (en)
Inventor
Carsten-Peter Carstens
Original Assignee
Stratagene California
Carsten-Peter Carstens
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene California, Carsten-Peter Carstens filed Critical Stratagene California
Application granted granted Critical
Publication of ATE434051T1 publication Critical patent/ATE434051T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Lubricants (AREA)
AT00914444T 1999-01-27 2000-01-27 Hohe expression eines heterologen proteins mit seltenen codons ATE434051T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11735599P 1999-01-27 1999-01-27
PCT/US2000/002002 WO2000044926A1 (en) 1999-01-27 2000-01-27 High level expression of a heterologous protein having rare codons

Publications (1)

Publication Number Publication Date
ATE434051T1 true ATE434051T1 (de) 2009-07-15

Family

ID=22372458

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00914444T ATE434051T1 (de) 1999-01-27 2000-01-27 Hohe expression eines heterologen proteins mit seltenen codons

Country Status (6)

Country Link
US (1) US20040214281A1 (de)
EP (1) EP1147211B1 (de)
AT (1) ATE434051T1 (de)
CA (1) CA2360352A1 (de)
DE (1) DE60042396D1 (de)
WO (1) WO2000044926A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2221600A (en) 1998-12-31 2000-07-31 Chiron Corporation Improved expression of hiv polypeptides and production of virus-like particles
AU2487300A (en) 1998-12-31 2000-07-31 Chiron Corporation Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof
AU2001249170A1 (en) * 2000-03-13 2001-09-24 Aptagen Method for modifying a nucleic acid
DE10037111A1 (de) * 2000-07-27 2002-02-07 Boehringer Ingelheim Int Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle
US6821755B2 (en) 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
EP1411770A4 (de) 2001-07-05 2006-05-10 Chiron Corp Polynukleotide, die für antigene hiv-polypeptide des c-typs codieren, polypeptide und ihre verwendung
US20030170614A1 (en) 2001-08-31 2003-09-11 Megede Jan Zur Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides and uses thereof
WO2003083101A1 (fr) * 2002-03-29 2003-10-09 Mitsubishi Pharma Corporation Cristaux de dap-kinase, procede de criblage d'une dap-kinase candidate ciblant un medicament au moyen de ces cristaux et medicament ainsi obtenu
JP2006500927A (ja) 2002-09-13 2006-01-12 ザ・ユニバーシティ・オブ・クイーンズランド コドン翻訳効率に基づく遺伝子発現系
WO2006055836A2 (en) * 2004-11-16 2006-05-26 President And Fellows Of Harvard College In vivo alteration of cellular dna
DE602007007612D1 (de) * 2006-04-22 2010-08-19 Scarab Genomics Llc Verfahren und zusammensetzungen zur herstellung rekombinanter proteine unter verwendung eines gens für trna
US8361746B2 (en) * 2008-07-24 2013-01-29 Brookhaven Science Associates, Llc Methods for detection of methyl-CpG dinucleotides
KR101488110B1 (ko) 2013-11-29 2015-01-29 성균관대학교산학협력단 나노아케움 이퀴탄스 유래의 Neq HS DNA 중합효소의 돌연변이체 제조 및 이를 이용한 hot-start PCR 응용
US10724040B2 (en) * 2015-07-15 2020-07-28 The Penn State Research Foundation mRNA sequences to control co-translational folding of proteins
CN105274042B (zh) * 2015-11-24 2019-05-21 山东省医学科学院基础医学研究所 一株重组大肠杆菌及其在发酵生产2[4Fe4S]铁氧还蛋白中的应用
CN105349480B (zh) * 2015-11-24 2019-03-05 山东省医学科学院基础医学研究所 一株高效表达[2Fe2S]铁氧还蛋白的重组大肠杆菌及其应用
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU618640B2 (en) * 1988-11-11 1992-01-02 Boehringer Mannheim Gmbh Process for the expression of a recombinant gene
DE4417598A1 (de) * 1994-05-19 1995-12-14 Max Planck Gesellschaft Verwendung des Tetracyclinpromotors zur stringent regulierten Produktion von rekombinanten Proteinen in prokaryontischen Zellen
US6013505A (en) * 1996-10-08 2000-01-11 Smithkline Beecham Corporation Topoisomerase I

Also Published As

Publication number Publication date
EP1147211A1 (de) 2001-10-24
EP1147211A4 (de) 2002-08-21
DE60042396D1 (de) 2009-07-30
EP1147211B1 (de) 2009-06-17
WO2000044926A1 (en) 2000-08-03
US20040214281A1 (en) 2004-10-28
CA2360352A1 (en) 2000-08-03

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