ATE437964T1 - Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation - Google Patents

Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation

Info

Publication number
ATE437964T1
ATE437964T1 AT04751627T AT04751627T ATE437964T1 AT E437964 T1 ATE437964 T1 AT E437964T1 AT 04751627 T AT04751627 T AT 04751627T AT 04751627 T AT04751627 T AT 04751627T AT E437964 T1 ATE437964 T1 AT E437964T1
Authority
AT
Austria
Prior art keywords
sense
double stranded
rna polymerase
rna
oligodeoxynucleotide tail
Prior art date
Application number
AT04751627T
Other languages
English (en)
Inventor
Robert Getts
Jaime Schwalm
Original Assignee
Genisphere Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genisphere Inc filed Critical Genisphere Inc
Application granted granted Critical
Publication of ATE437964T1 publication Critical patent/ATE437964T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT04751627T 2003-05-09 2004-05-07 Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation ATE437964T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46938303P 2003-05-09 2003-05-09
PCT/US2004/014325 WO2004101749A2 (en) 2003-05-09 2004-05-07 Methods for amplification of nucleic acid sequences using staggered ligation

Publications (1)

Publication Number Publication Date
ATE437964T1 true ATE437964T1 (de) 2009-08-15

Family

ID=33452281

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04751627T ATE437964T1 (de) 2003-05-09 2004-05-07 Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation

Country Status (7)

Country Link
US (1) US7494789B2 (de)
EP (1) EP1623044B1 (de)
JP (1) JP4599483B2 (de)
AT (1) ATE437964T1 (de)
AU (1) AU2004239283B2 (de)
DE (1) DE602004022282D1 (de)
WO (1) WO2004101749A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1730314B1 (de) * 2004-04-01 2009-10-21 Genisphere Inc. Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
US7550264B2 (en) * 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
US20070105124A1 (en) 2005-11-08 2007-05-10 Getts Robert C Methods and kits for nucleic acid amplification
US7998423B2 (en) 2007-02-27 2011-08-16 Basf Corporation SCR on low thermal mass filter substrates
US20110306099A1 (en) * 2008-11-03 2011-12-15 Matthew Beasley Method of cloning dna
LT2825672T (lt) 2012-03-13 2019-06-10 Swift Biosciences, Inc. Būdai ir kompozicijos, skirti valdomam pagal dydį substrato polinukleotidų homopolimeriniam uodegų formavimui su nukleorūgščių polimeraze
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
KR100440044B1 (ko) * 1998-02-27 2004-07-14 팸진 비.브이. 핵산의 비특이적 증폭방법
US20020102589A1 (en) 2000-10-13 2002-08-01 Ryoiti Kiyama Microarrays and methods for evaluating activity of compounds having estrogen-like activity
JP2002360249A (ja) * 2000-10-13 2002-12-17 National Institute Of Advanced Industrial & Technology マイクロアレイ及びエストロゲン類似活性化合物の活性評価方法
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
GB0123401D0 (en) 2001-09-28 2001-11-21 Novartis Forschungsstiftung Methods of inducing gene expression
EP1730314B1 (de) 2004-04-01 2009-10-21 Genisphere Inc. Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates

Also Published As

Publication number Publication date
EP1623044A4 (de) 2007-11-07
WO2004101749A3 (en) 2005-02-10
AU2004239283A1 (en) 2004-11-25
US7494789B2 (en) 2009-02-24
AU2004239283B2 (en) 2010-01-28
JP2007515938A (ja) 2007-06-21
EP1623044B1 (de) 2009-07-29
US20070072182A1 (en) 2007-03-29
EP1623044A2 (de) 2006-02-08
JP4599483B2 (ja) 2010-12-15
DE602004022282D1 (de) 2009-09-10
WO2004101749A2 (en) 2004-11-25

Similar Documents

Publication Publication Date Title
WO2006081222A3 (en) Isothermal dna amplification
WO2007017229A3 (de) Verfahren für die kontinuierliche zielgerichtete evolution von proteinen in vitro
JP2017537626A5 (de)
WO2023201281A3 (en) Oligonucleotides for atn1 modulation
WO2004007718A3 (en) Rna-interference by single-stranded rna molecules
AU2008339215A8 (en) Novel siRNA structure for minimizing off-target effects and relaxing saturation of RNAi machinery and the use thereof
SI2258847T1 (sl) Nadaljnje nove oblike molekul interferenčne RNA
DK1934345T3 (da) Modifikationer af rna, der fører til en forøget transkriptstabilitet og translationseffektivitet
WO2004013280A3 (en) ALLELE-SPECIFIC siRNA-MEDIATED GENE SILENCING
DE50206993D1 (de) Verfahren zur hemmung der expression eine zielgens
DE60227525D1 (de) Verfahren zur Herstellung von Nukleinsäuremolekülen
DE69827745D1 (de) Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase
ATE528402T1 (de) Effiziente genabschaltung in pflanzen unter verwendung von kurzen dsrna-sequenzen
WO2003057840A3 (en) Compositions for dna mediated gene silencing
CO6241167A2 (es) Arn circular monocatenario y metodo para producirlo
ATE464381T1 (de) Rna-amplifikation auf ligationsbasis
JP2024534945A5 (de)
WO2004065600A3 (en) Rna interference by palindromic or modified rna molecules
ATE437964T1 (de) Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation
WO2003102232A3 (de) Vermehrung von ribonukleinsäuren
BR112022014513A2 (pt) Agentes caotrópicos para reduzir a formação de rna de fita dupla
WO2005042705A3 (en) Short interfering rna and micro-rna compounds and methods of designing, making and using the same
ATE539167T1 (de) Verfahren zur amplifikation von rna-sequenzen
MX2007004686A (es) Moleculas de arn de multidominio que comprenden al menos un aptamero para suministrar arn de doble hebra a organismos nocivos.
JP2007515938A5 (de)

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties