ATE438093T1 - Verfahren zur charakterisierung einzelner polymere - Google Patents

Verfahren zur charakterisierung einzelner polymere

Info

Publication number
ATE438093T1
ATE438093T1 AT02737289T AT02737289T ATE438093T1 AT E438093 T1 ATE438093 T1 AT E438093T1 AT 02737289 T AT02737289 T AT 02737289T AT 02737289 T AT02737289 T AT 02737289T AT E438093 T1 ATE438093 T1 AT E438093T1
Authority
AT
Austria
Prior art keywords
determining
molecules
velocity
methods
length
Prior art date
Application number
AT02737289T
Other languages
English (en)
Inventor
Eugene Chan
Lance Gleich
Parris Wellman
Original Assignee
Us Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Genomics Inc filed Critical Us Genomics Inc
Application granted granted Critical
Publication of ATE438093T1 publication Critical patent/ATE438093T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size
    • G01N2015/1495—Deformation of particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/44—Resins; Plastics; Rubber; Leather
    • G01N33/442—Resins; Plastics
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Polymerisation Methods In General (AREA)
  • Length Measuring Devices With Unspecified Measuring Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT02737289T 2001-06-06 2002-05-31 Verfahren zur charakterisierung einzelner polymere ATE438093T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/875,779 US6927065B2 (en) 1999-08-13 2001-06-06 Methods and apparatus for characterization of single polymers
PCT/US2002/017124 WO2002099398A1 (en) 2001-06-06 2002-05-31 Methods and apparatus for characterization of single polymers

Publications (1)

Publication Number Publication Date
ATE438093T1 true ATE438093T1 (de) 2009-08-15

Family

ID=25366347

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02737289T ATE438093T1 (de) 2001-06-06 2002-05-31 Verfahren zur charakterisierung einzelner polymere

Country Status (6)

Country Link
US (1) US6927065B2 (de)
EP (1) EP1402245B1 (de)
JP (1) JP2004537045A (de)
AT (1) ATE438093T1 (de)
DE (1) DE60233138D1 (de)
WO (1) WO2002099398A1 (de)

Families Citing this family (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
DE69825601T2 (de) 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline Verfahren zur analyse von polymeren
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1192453B1 (de) 1999-06-22 2012-02-15 President and Fellows of Harvard College Bestimmung von biopolymeren entsprechend der molekularen oder atomaren skala
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
CA2440754A1 (en) 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
EP1402069A4 (de) * 2001-06-08 2006-01-25 Us Genomics Inc Verfahren und produkte zur nukleinsäureanalyse mittels nick-translation
US20030104428A1 (en) * 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
AU2002337653A1 (en) * 2001-07-25 2003-02-17 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
US9678038B2 (en) 2001-07-25 2017-06-13 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
AU2002336613A1 (en) * 2001-09-18 2003-04-01 U.S. Genomics, Inc. Differential tagging of polymers for high resolution linear analysis
WO2003106693A2 (en) 2002-01-01 2003-12-24 Princeton University Gradient structures interfacing microfluidics and nanofluidics, methods for fabrication and uses thereof
US7652574B2 (en) * 2002-04-08 2010-01-26 Sayegh Adel O Article surveillance tag having a vial
WO2003091455A1 (en) * 2002-04-23 2003-11-06 U.S.Genomics, Inc. Compositions and methods related to two-arm nucleic acid probes
EP1540017A4 (de) * 2002-05-09 2006-05-24 Us Genomics Inc Verfahren zur analyse einer nukleinsäure
WO2003100101A1 (en) * 2002-05-28 2003-12-04 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US7282330B2 (en) * 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US20040053399A1 (en) * 2002-07-17 2004-03-18 Rudolf Gilmanshin Methods and compositions for analyzing polymers using chimeric tags
US20040110205A1 (en) * 2002-09-23 2004-06-10 Hui Wang Methods and systems for nanopore data analysis
EP1586068B1 (de) * 2003-01-23 2008-07-30 U.S. Genomics, Inc. Verfahren zur analyse von polymer-populationen
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
WO2005017205A2 (en) * 2003-08-04 2005-02-24 U. S. Genomics, Inc. Nucleic acid mapping using linear analysis
WO2005017025A2 (en) 2003-08-15 2005-02-24 The President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
WO2005022162A1 (en) * 2003-08-21 2005-03-10 U.S. Genomics, Inc. Quantum dots and methods of use thereof
WO2005047470A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Intercalator fret donors or acceptors
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7595160B2 (en) * 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
EP1704256A4 (de) * 2004-01-13 2008-01-16 Us Genomics Inc Nachweis und quantifizierung von analyten in lösung unter verwendung von polymeren
US20050196790A1 (en) * 2004-02-05 2005-09-08 U.S. Genomics, Inc. Methods for detection and quantitation of minimum length polymers
EP1716254B1 (de) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Verfahren zur analyse von polynukleotidsequenzen
US20050221408A1 (en) * 2004-03-19 2005-10-06 U.S. Genomics, Inc. Compositions and methods for detection of single molecules
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
EP1766090B1 (de) 2004-05-25 2011-04-27 Helicos Biosciences Corporation Verfahren zur nukleinsäureimmobilisierung
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
WO2006017274A2 (en) * 2004-07-13 2006-02-16 U.S. Genomics, Inc. Systems and methods for sample modification using fluidic chambers
US7351538B2 (en) * 2004-08-23 2008-04-01 U.S. Genomics Systems and methods for detecting and analyzing polymers
US7262859B2 (en) * 2004-10-13 2007-08-28 U.S. Genomics, Inc. Systems and methods for measurement optimization
WO2006044728A2 (en) * 2004-10-18 2006-04-27 U.S.Genomics, Inc. Methods for isolation of nucleic acids from prokaryotic spores
US20060160231A1 (en) * 2004-11-24 2006-07-20 U.S. Genomics, Inc. Linear analysis of polymers
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20070128083A1 (en) * 2005-07-18 2007-06-07 U.S. Genomics, Inc. Microfluidic methods and apparatuses for sample preparation and analysis
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007056250A2 (en) * 2005-11-04 2007-05-18 U.S. Genomics, Inc. Heterogeneous assay of analytes in solution using polymers
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP3978121A1 (de) 2006-07-19 2022-04-06 BioNano Genomics, Inc. Nanodüsen-geräteanordnungen: ihre herstellung und verwendung zur makromolekularen analyse
US20080032410A1 (en) * 2006-07-19 2008-02-07 Bio-Rad Laboratories, Inc. Labeling for identification of proteins and other macromolecules
US7985542B2 (en) * 2006-09-07 2011-07-26 Institut Pasteur Genomic morse code
WO2008085991A2 (en) 2007-01-08 2008-07-17 U.S. Genomics, Inc. Reaction chamber
US20090136932A1 (en) * 2007-03-16 2009-05-28 Craighead Harold G Fibers with isolated biomolecules and uses thereof
CA2964611C (en) * 2007-03-28 2021-06-01 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
WO2009046094A1 (en) * 2007-10-01 2009-04-09 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
WO2009092035A2 (en) 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
AU2009256064B2 (en) 2008-06-06 2015-04-30 Bionano Genomics, Inc. Integrated nanofluidic analysis devices, fabrication methods and analysis techniques
CA2729159C (en) 2008-06-30 2020-01-14 Bionanomatrix, Inc. Methods and devices for single-molecule whole genome analysis
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
WO2010028140A2 (en) 2008-09-03 2010-03-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8361716B2 (en) 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
US9181578B2 (en) 2008-11-18 2015-11-10 Bionano Genomics, Inc. Polynucleotide mapping and sequencing
US8455260B2 (en) 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
US8246799B2 (en) 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US9605298B2 (en) 2009-08-06 2017-03-28 Cornell University Device and methods for molecular analysis
WO2011030944A1 (ko) * 2009-09-14 2011-03-17 한국과학기술연구원 다중 pna를 이용하는 dna 서열 분석 방법 및 장치
EP2580588B1 (de) 2010-06-08 2014-09-24 President and Fellows of Harvard College Nanoporen-vorrichtung mit graphengetragener künstlicher lipidmembran
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
AU2011316989B2 (en) * 2010-10-20 2016-06-16 Bionano Genomics, Inc. Systems and methods for assessing biomolecule characteristics
EP2640849B1 (de) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Verfahren zur sequenzierung von biomolekülen durch nachweis der relativen positionen von hybridisierten sonden
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
JP5853331B2 (ja) * 2011-03-11 2016-02-09 株式会社ブイ・テクノロジー レーザ照射装置及びそれを使用した液晶表示パネルの輝点修正方法
US8956815B2 (en) 2012-04-18 2015-02-17 Toxic Report Llc Intercalation methods and devices
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
WO2014113557A1 (en) 2013-01-18 2014-07-24 Nabsys, Inc. Enhanced probe binding
US10443092B2 (en) * 2013-03-13 2019-10-15 President And Fellows Of Harvard College Methods of elongating DNA
US9182420B2 (en) * 2013-03-15 2015-11-10 Palo Alto Research Center Incorporated Phase-change enabled flow field visualization
US9946918B2 (en) 2015-11-16 2018-04-17 MorphoTrak, LLC Symbol detection for desired image reconstruction
WO2017210505A1 (en) * 2016-06-03 2017-12-07 Verily Life Sciences Llc Multichannel excitation and emission for miniaturized, planar fluorescence activated cell sorting
US10379038B2 (en) 2016-10-06 2019-08-13 Government Of The United States Of America, As Represented By The Secretary Of Commerce Measuring a size distribution of nucleic acid molecules in a sample
US10731210B2 (en) 2017-02-01 2020-08-04 Seagate Technology Llc Fabrication of nanochannel with integrated electrodes for DNA sequencing using tunneling current
US10655164B2 (en) * 2017-02-01 2020-05-19 Seagate Technology Llc Single molecule DNA sequencing method using confined nano-fluidic channel and sub-nanometer electrode gap
US10641726B2 (en) 2017-02-01 2020-05-05 Seagate Technology Llc Fabrication of a nanochannel for DNA sequencing using electrical plating to achieve tunneling electrode gap
US10889857B2 (en) 2017-02-01 2021-01-12 Seagate Technology Llc Method to fabricate a nanochannel for DNA sequencing based on narrow trench patterning process
US10761058B2 (en) 2017-02-01 2020-09-01 Seagate Technology Llc Nanostructures to control DNA strand orientation and position location for transverse DNA sequencing
US10640827B2 (en) 2017-02-01 2020-05-05 Seagate Technology Llc Fabrication of wedge shaped electrode for enhanced DNA sequencing using tunneling current
US10752947B2 (en) 2017-03-09 2020-08-25 Seagate Technology Llc Method to amplify transverse tunneling current discrimination of DNA nucleotides via nucleotide site specific attachment of dye-peptide
US20180259475A1 (en) 2017-03-09 2018-09-13 Seagate Technology Llc Vertical nanopore coupled with a pair of transverse electrodes having a uniform ultrasmall nanogap for dna sequencing
CN110741093A (zh) * 2017-04-28 2020-01-31 渥太华大学 控制通过纳米孔的分子的移位
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
EP3936856A1 (de) * 2020-07-10 2022-01-12 Vrije Universiteit Brussel Schaltung und verfahren zur fluoreszenzlebensdauerabbildung

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4573796A (en) * 1984-01-06 1986-03-04 The United States Of America As Represented By The United States Department Of Energy Apparatus for eliminating background interference in fluorescence measurements
DE69025969T2 (de) 1989-04-05 1996-08-08 New York University, New York, N.Y. Verfahren zur Charakterisierung von Teilchen
US4979824A (en) * 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5079169A (en) 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
JP3123249B2 (ja) * 1991-09-10 2001-01-09 株式会社日立製作所 Ｄｎａ分子の長さ計測方法および計測装置
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
AU4238593A (en) 1992-05-07 1993-11-29 Ig Laboratories, Inc. Methods for high resolution gene mapping
WO1994016104A1 (en) * 1993-01-08 1994-07-21 Ctrc Research Foundation Color imaging system for use in molecular biology
US5427663A (en) 1993-06-08 1995-06-27 British Technology Group Usa Inc. Microlithographic array for macromolecule and cell fractionation
FR2716263B1 (fr) 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US5538898A (en) * 1995-03-16 1996-07-23 International Business Machines Corporation Method suitable for identifying a code sequence of a biomolecule
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
AU5525296A (en) 1995-03-24 1996-10-16 Ely Michael Rabani Assembly of complex molecular and supramolecular objects and devices and uses thereof
ES2296297T3 (es) 1995-08-09 2008-04-16 Adam J. Simon Orientacion molecular de macromoleculas mediante la accion de un menisco sobre superficies altamente especificas.
US5846832A (en) 1995-09-20 1998-12-08 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for shear breakage of polynucleotides
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
WO1998010097A2 (en) 1996-09-03 1998-03-12 Pioneer Hi-Bred International, Inc. Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
DE69825601T2 (de) 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline Verfahren zur analyse von polymeren
WO1999005315A2 (en) 1997-07-28 1999-02-04 Medical Biosystems Ltd. Nucleic acid sequence analysis
US7098012B1 (en) 1997-11-12 2006-08-29 Mcgill University DNA demethylase, therapeutic and diagnostic uses thereof
WO2000006587A1 (en) 1998-07-29 2000-02-10 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. New cofactors for methyltransferases
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
JP2002522780A (ja) 1998-08-13 2002-07-23 ユー．エス．ゲノミクス ポリマーの光学的な特性決定
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
EP1210578A4 (de) 1999-08-13 2007-02-21 U S Genomics Verfahren und apparate zur dehnung von polymeren
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6762059B2 (en) 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
EP1402071A4 (de) 2001-06-08 2005-12-14 Us Genomics Inc Verfahren und produkte zur analyse von nukleinsäuren auf grundlage des methylierungsstatus
EP1402069A4 (de) 2001-06-08 2006-01-25 Us Genomics Inc Verfahren und produkte zur nukleinsäureanalyse mittels nick-translation
AU2002336613A1 (en) 2001-09-18 2003-04-01 U.S. Genomics, Inc. Differential tagging of polymers for high resolution linear analysis
WO2003091455A1 (en) 2002-04-23 2003-11-06 U.S.Genomics, Inc. Compositions and methods related to two-arm nucleic acid probes
EP1540017A4 (de) 2002-05-09 2006-05-24 Us Genomics Inc Verfahren zur analyse einer nukleinsäure
WO2003100101A1 (en) 2002-05-28 2003-12-04 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US20040053399A1 (en) 2002-07-17 2004-03-18 Rudolf Gilmanshin Methods and compositions for analyzing polymers using chimeric tags
EP1586068B1 (de) 2003-01-23 2008-07-30 U.S. Genomics, Inc. Verfahren zur analyse von polymer-populationen

Also Published As

Publication number Publication date
US6927065B2 (en) 2005-08-09
WO2002099398A1 (en) 2002-12-12
US20020039737A1 (en) 2002-04-04
EP1402245A1 (de) 2004-03-31
EP1402245B1 (de) 2009-07-29
DE60233138D1 (de) 2009-09-10
JP2004537045A (ja) 2004-12-09
EP1402245A4 (de) 2006-03-22

Similar Documents

Publication Publication Date Title
DE60233138D1 (de) Verfahren zur Charakterisierung einzelner Polymere
EP3596906A4 (de) System und verfahren zur blockchain-basierten authentifizierung
GB0031564D0 (en) Viscosity measurement
DE69825601D1 (de) Verfahren zur analyse von polymeren
EP4271015A4 (de) Registrierungsverfahren und -vorrichtung, authentifizierungsverfahren und -vorrichtung, routingindikatorbestimmungsverfahren und -vorrichtung, einheit und endgerät
ATE376195T1 (de) Verfahren und vorrichtung zur bestimmung von abstand und relativgeschwindigkeit eines entfernten objektes
DE10291293T1 (de) Vorrichtung und Verfahren zum Messen und Schätzen der Wahrscheinlichkeit von Taktschrägen
ATE489779T1 (de) Verfahren und vorrichtung zur schätzung der dopplerspreizung
AT5911U3 (de) Verfahren zur kontaktfreien messung eines querprofils bzw. abstands von schienen eines gleises
DK1454164T3 (da) Fremgangsmåde og anordning til geometrisk opmåling og hastighedsbestemmelse af köretöjer
ATE421791T1 (de) Verfahren und vorrichtung zur arcerkennung in einem plasmaprozess
DE60044713D1 (de) Verfahren und Vorrichtung zur gleichzeitigen Prüfung einer Vielzahl von differenziellen Druckmesssensoren
EP4503493A4 (de) Verfahren und vorrichtung zur bestimmung der beziehung zwischen ptrs-ports und dmrs-ports, medium und produkt
ATE335186T1 (de) Verfahren und vorrichtung zur erfassung von auf einem fördermittel bewegten objekten mittels eines optoelektronischen sensors
ATE328121T1 (de) Nachweisbares kationisches flockungsmittel sowie verfahren zur verwendung desselben in industriellen lebensmittelprozessen
DE60218877D1 (de) Verfahren und vorrichtung zur messung des aldehyd-gehalts in einem polyester-polymer
EP4568358A4 (de) Verfahren zur bestimmung von aufwecksignalinformationen, endgerät und netzwerkseitige vorrichtung
DE60202656D1 (de) Verfahren und Vorrichtung zur Ermittlung von Frequenzdifferenzen, und Phasenregelkreis
EP4383874A4 (de) Verfahren und vorrichtung zum senden und empfangen eines signals und kommunikationssystem
EP3803355A4 (de) Prüfsystem für eine elektronische vorrichtung, dieses enthaltende system zur herstellung elektronischer vorrichtungen und verfahren zum testen einer elektronischen vorrichtung
ATE414917T1 (de) Verfahren zur bestimmung der position und geschwindigkeit von targets aus durch die targets gestreuten signalen
ATE442629T1 (de) Verfahren und system zum identifizieren von elektronischen dateien
DE602004015454D1 (de) Verfahren und vorrichtung zur kontinuierlichen radioaktiven markierung von proteinen im grossen massstab
DE60124262D1 (de) Vorrichtung und verfahren zur charakterisierung und sequenzierung von polymeren
EP3841504A4 (de) System und verfahren zur erkennung von cyberangriffen basierend auf einer schnellen unüberwachten erkennung von wiederkehrenden signalmustern

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties