ATE438093T1 - Verfahren zur charakterisierung einzelner polymere - Google Patents

Verfahren zur charakterisierung einzelner polymere

Info

Publication number
ATE438093T1
ATE438093T1 AT02737289T AT02737289T ATE438093T1 AT E438093 T1 ATE438093 T1 AT E438093T1 AT 02737289 T AT02737289 T AT 02737289T AT 02737289 T AT02737289 T AT 02737289T AT E438093 T1 ATE438093 T1 AT E438093T1
Authority
AT
Austria
Prior art keywords
determining
molecules
velocity
methods
length
Prior art date
Application number
AT02737289T
Other languages
English (en)
Inventor
Eugene Chan
Lance Gleich
Parris Wellman
Original Assignee
Us Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Genomics Inc filed Critical Us Genomics Inc
Application granted granted Critical
Publication of ATE438093T1 publication Critical patent/ATE438093T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size
    • G01N2015/1495—Deformation of particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/44—Resins; Plastics; Rubber; Leather
    • G01N33/442—Resins; Plastics
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Polymerisation Methods In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Length Measuring Devices With Unspecified Measuring Means (AREA)
AT02737289T 2001-06-06 2002-05-31 Verfahren zur charakterisierung einzelner polymere ATE438093T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/875,779 US6927065B2 (en) 1999-08-13 2001-06-06 Methods and apparatus for characterization of single polymers
PCT/US2002/017124 WO2002099398A1 (en) 2001-06-06 2002-05-31 Methods and apparatus for characterization of single polymers

Publications (1)

Publication Number Publication Date
ATE438093T1 true ATE438093T1 (de) 2009-08-15

Family

ID=25366347

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02737289T ATE438093T1 (de) 2001-06-06 2002-05-31 Verfahren zur charakterisierung einzelner polymere

Country Status (6)

Country Link
US (1) US6927065B2 (de)
EP (1) EP1402245B1 (de)
JP (1) JP2004537045A (de)
AT (1) ATE438093T1 (de)
DE (1) DE60233138D1 (de)
WO (1) WO2002099398A1 (de)

Families Citing this family (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
EP1009802B1 (de) 1997-02-12 2004-08-11 Eugene Y. Chan Verfahren zur analyse von polymeren
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
AU5631200A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Control of solid state dimensional features
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
EP1402069A4 (de) * 2001-06-08 2006-01-25 Us Genomics Inc Verfahren und produkte zur nukleinsäureanalyse mittels nick-translation
US20030104428A1 (en) * 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
WO2003010289A2 (en) * 2001-07-25 2003-02-06 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
US9678038B2 (en) 2001-07-25 2017-06-13 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
AU2002336613A1 (en) * 2001-09-18 2003-04-01 U.S. Genomics, Inc. Differential tagging of polymers for high resolution linear analysis
US7652574B2 (en) * 2002-04-08 2010-01-26 Sayegh Adel O Article surveillance tag having a vial
AU2003269813A1 (en) 2002-04-16 2003-12-31 Princeton University Gradient structures interfacing microfluidics and nanofluidics, methods for fabrication and uses thereof
AU2003228649A1 (en) * 2002-04-23 2003-11-10 U.S.Genomics, Inc. Compositions and methods related to two-arm nucleic acid probes
US20030235854A1 (en) * 2002-05-09 2003-12-25 Chan Eugene Y. Methods for analyzing a nucleic acid
AU2003238796A1 (en) * 2002-05-28 2003-12-12 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US7282330B2 (en) * 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
WO2004007692A2 (en) * 2002-07-17 2004-01-22 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
US20040110205A1 (en) * 2002-09-23 2004-06-10 Hui Wang Methods and systems for nanopore data analysis
EP1586068B1 (de) * 2003-01-23 2008-07-30 U.S. Genomics, Inc. Verfahren zur analyse von polymer-populationen
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
WO2005017205A2 (en) * 2003-08-04 2005-02-24 U. S. Genomics, Inc. Nucleic acid mapping using linear analysis
WO2005017025A2 (en) 2003-08-15 2005-02-24 The President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
US20050042665A1 (en) * 2003-08-21 2005-02-24 U.S. Genomics, Inc. Quantum dots and methods of use thereof
WO2005047470A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Intercalator fret donors or acceptors
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005067692A2 (en) * 2004-01-13 2005-07-28 U.S. Genomics, Inc. Detection and quantification of analytes in solution using polymers
US7595160B2 (en) * 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US20050196790A1 (en) * 2004-02-05 2005-09-08 U.S. Genomics, Inc. Methods for detection and quantitation of minimum length polymers
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
EP1725587A2 (de) * 2004-03-19 2006-11-29 U.S. Genomics, Inc. Zusammensetzungen und verfahren zum nachweis von einzelnen molekülen
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
CA2566806A1 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
WO2006017274A2 (en) * 2004-07-13 2006-02-16 U.S. Genomics, Inc. Systems and methods for sample modification using fluidic chambers
EP1786932A4 (de) * 2004-08-23 2010-10-27 Us Genomics Inc Systeme und verfahren zur detektion und analyse von polymeren
US7262859B2 (en) * 2004-10-13 2007-08-28 U.S. Genomics, Inc. Systems and methods for measurement optimization
US7888011B2 (en) * 2004-10-18 2011-02-15 U.S. Genomics, Inc. Methods for isolation of nucleic acids from prokaryotic spores
US20060160231A1 (en) * 2004-11-24 2006-07-20 U.S. Genomics, Inc. Linear analysis of polymers
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20070128083A1 (en) * 2005-07-18 2007-06-07 U.S. Genomics, Inc. Microfluidic methods and apparatuses for sample preparation and analysis
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007056250A2 (en) * 2005-11-04 2007-05-18 U.S. Genomics, Inc. Heterogeneous assay of analytes in solution using polymers
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP2049262A2 (de) 2006-07-19 2009-04-22 Bionanomatrix, Inc. Nanodüsen-geräteanordnungen: ihre herstellung und verwendung zur makromolekularen analyse
US20080032410A1 (en) * 2006-07-19 2008-02-07 Bio-Rad Laboratories, Inc. Labeling for identification of proteins and other macromolecules
US7985542B2 (en) * 2006-09-07 2011-07-26 Institut Pasteur Genomic morse code
WO2008085991A2 (en) 2007-01-08 2008-07-17 U.S. Genomics, Inc. Reaction chamber
US20090136932A1 (en) * 2007-03-16 2009-05-28 Craighead Harold G Fibers with isolated biomolecules and uses thereof
CA2964611C (en) 2007-03-28 2021-06-01 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
WO2009046094A1 (en) 2007-10-01 2009-04-09 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
WO2009092035A2 (en) * 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
KR20170094003A (ko) * 2008-06-06 2017-08-16 바이오나노 제노믹스, 인크. 통합 나노유체 분석 장치, 제작 방법 및 분석 기술
CA3062190C (en) 2008-06-30 2023-03-28 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
US8882980B2 (en) 2008-09-03 2014-11-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US8361716B2 (en) 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
AU2009316628B2 (en) 2008-11-18 2016-06-16 Bionano Genomics, Inc. Polynucleotide mapping and sequencing
US8455260B2 (en) 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
US8246799B2 (en) 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
EP2462245B1 (de) 2009-08-06 2016-10-05 Cornell University Vorrichtung und verfahren für epigenetische analyse
US20120282709A1 (en) * 2009-09-14 2012-11-08 Byung Chul Lee Method and device for dna sequence analysis using multiple pna
WO2012005857A1 (en) 2010-06-08 2012-01-12 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
WO2012054735A2 (en) * 2010-10-20 2012-04-26 Bionano Genomics, Inc. Systems and methods for assessing biomolecule characteristics
EP2640849B1 (de) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Verfahren zur sequenzierung von biomolekülen durch nachweis der relativen positionen von hybridisierten sonden
WO2012109574A2 (en) 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
JP5853331B2 (ja) * 2011-03-11 2016-02-09 株式会社ブイ・テクノロジー レーザ照射装置及びそれを使用した液晶表示パネルの輝点修正方法
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
US8956815B2 (en) 2012-04-18 2015-02-17 Toxic Report Llc Intercalation methods and devices
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
WO2014113557A1 (en) 2013-01-18 2014-07-24 Nabsys, Inc. Enhanced probe binding
WO2014164716A1 (en) * 2013-03-13 2014-10-09 President And Fellows Of Harvard College Methods of elongating dna
US9182420B2 (en) * 2013-03-15 2015-11-10 Palo Alto Research Center Incorporated Phase-change enabled flow field visualization
US9946918B2 (en) * 2015-11-16 2018-04-17 MorphoTrak, LLC Symbol detection for desired image reconstruction
US11828697B2 (en) 2016-06-03 2023-11-28 Verily Life Sciences Llc Multichannel excitation and emission for miniaturized, planar fluorescence activated cell sorting
US10379038B2 (en) 2016-10-06 2019-08-13 Government Of The United States Of America, As Represented By The Secretary Of Commerce Measuring a size distribution of nucleic acid molecules in a sample
US10641726B2 (en) 2017-02-01 2020-05-05 Seagate Technology Llc Fabrication of a nanochannel for DNA sequencing using electrical plating to achieve tunneling electrode gap
US10655164B2 (en) * 2017-02-01 2020-05-19 Seagate Technology Llc Single molecule DNA sequencing method using confined nano-fluidic channel and sub-nanometer electrode gap
US10640827B2 (en) 2017-02-01 2020-05-05 Seagate Technology Llc Fabrication of wedge shaped electrode for enhanced DNA sequencing using tunneling current
US10889857B2 (en) 2017-02-01 2021-01-12 Seagate Technology Llc Method to fabricate a nanochannel for DNA sequencing based on narrow trench patterning process
US10731210B2 (en) 2017-02-01 2020-08-04 Seagate Technology Llc Fabrication of nanochannel with integrated electrodes for DNA sequencing using tunneling current
US10761058B2 (en) 2017-02-01 2020-09-01 Seagate Technology Llc Nanostructures to control DNA strand orientation and position location for transverse DNA sequencing
US10752947B2 (en) 2017-03-09 2020-08-25 Seagate Technology Llc Method to amplify transverse tunneling current discrimination of DNA nucleotides via nucleotide site specific attachment of dye-peptide
US20180259475A1 (en) 2017-03-09 2018-09-13 Seagate Technology Llc Vertical nanopore coupled with a pair of transverse electrodes having a uniform ultrasmall nanogap for dna sequencing
AU2018260787A1 (en) * 2017-04-28 2019-10-31 The University Of Ottawa Controlling translocating molecules through a nanopore
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
EP3936856A1 (de) * 2020-07-10 2022-01-12 Vrije Universiteit Brussel Schaltung und verfahren zur fluoreszenzlebensdauerabbildung

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4573796A (en) * 1984-01-06 1986-03-04 The United States Of America As Represented By The United States Department Of Energy Apparatus for eliminating background interference in fluorescence measurements
DE69025969T2 (de) 1989-04-05 1996-08-08 New York University, New York, N.Y. Verfahren zur Charakterisierung von Teilchen
US4979824A (en) * 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5079169A (en) 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
JP3123249B2 (ja) * 1991-09-10 2001-01-09 株式会社日立製作所 Ｄｎａ分子の長さ計測方法および計測装置
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
JPH07506259A (ja) 1992-05-07 1995-07-13 アイジー・ラボラトリーズ，インコーポレイテッド 高分解遺伝子マッピング法
WO1994016104A1 (en) * 1993-01-08 1994-07-21 Ctrc Research Foundation Color imaging system for use in molecular biology
US5427663A (en) 1993-06-08 1995-06-27 British Technology Group Usa Inc. Microlithographic array for macromolecule and cell fractionation
FR2716263B1 (fr) 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US5538898A (en) * 1995-03-16 1996-07-23 International Business Machines Corporation Method suitable for identifying a code sequence of a biomolecule
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1996030508A1 (en) 1995-03-24 1996-10-03 Ely Michael Rabani Assembly of complex molecular and supramolecular objects and devices and uses thereof
DE69637317T2 (de) 1995-08-09 2008-10-16 Simon, Adam J. Molekulare ausrichtung von makromolekuelen mit hilfe eines fluessigkeitsmeniskus auf einer hochspezifischen oberflaeche
US5846832A (en) 1995-09-20 1998-12-08 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for shear breakage of polynucleotides
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
WO1998010097A2 (en) 1996-09-03 1998-03-12 Pioneer Hi-Bred International, Inc. Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination
EP1009802B1 (de) 1997-02-12 2004-08-11 Eugene Y. Chan Verfahren zur analyse von polymeren
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
DK1017848T3 (da) 1997-07-28 2003-02-10 Medical Biosystems Ltd Nucleinsyresekvensanalyse
WO1999024583A1 (en) 1997-11-12 1999-05-20 Mcgill University Dna demethylase, therapeutic and diagnostic uses thereof
WO2000006587A1 (en) 1998-07-29 2000-02-10 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. New cofactors for methyltransferases
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
CA2340228A1 (en) 1998-08-13 2000-02-24 U.S. Genomics, Inc. Optically characterizing polymers
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
AU6771100A (en) 1999-08-13 2001-03-13 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6762059B2 (en) 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
AU2002318336A1 (en) 2001-06-08 2002-12-23 U.S. Genomics, Inc. Methods and products for analyzing nucleic acids based on methylation status
EP1402069A4 (de) 2001-06-08 2006-01-25 Us Genomics Inc Verfahren und produkte zur nukleinsäureanalyse mittels nick-translation
AU2002336613A1 (en) 2001-09-18 2003-04-01 U.S. Genomics, Inc. Differential tagging of polymers for high resolution linear analysis
AU2003228649A1 (en) 2002-04-23 2003-11-10 U.S.Genomics, Inc. Compositions and methods related to two-arm nucleic acid probes
US20030235854A1 (en) 2002-05-09 2003-12-25 Chan Eugene Y. Methods for analyzing a nucleic acid
AU2003238796A1 (en) 2002-05-28 2003-12-12 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
WO2004007692A2 (en) 2002-07-17 2004-01-22 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
EP1586068B1 (de) 2003-01-23 2008-07-30 U.S. Genomics, Inc. Verfahren zur analyse von polymer-populationen

Also Published As

Publication number Publication date
US6927065B2 (en) 2005-08-09
JP2004537045A (ja) 2004-12-09
DE60233138D1 (de) 2009-09-10
EP1402245A4 (de) 2006-03-22
US20020039737A1 (en) 2002-04-04
EP1402245A1 (de) 2004-03-31
WO2002099398A1 (en) 2002-12-12
EP1402245B1 (de) 2009-07-29

Similar Documents

Publication Publication Date Title
DE60233138D1 (de) Verfahren zur Charakterisierung einzelner Polymere
EP3596906A4 (de) System und verfahren zur blockchain-basierten authentifizierung
GB0031564D0 (en) Viscosity measurement
ATE273381T1 (de) Verfahren zur analyse von polymeren
EP4271015A4 (de) Registrierungsverfahren und -vorrichtung, authentifizierungsverfahren und -vorrichtung, routingindikatorbestimmungsverfahren und -vorrichtung, einheit und endgerät
ATE376195T1 (de) Verfahren und vorrichtung zur bestimmung von abstand und relativgeschwindigkeit eines entfernten objektes
DE10291293T1 (de) Vorrichtung und Verfahren zum Messen und Schätzen der Wahrscheinlichkeit von Taktschrägen
ATE372089T1 (de) Analyseverfahren von unregelmässigkeiten in menschlicher fortbewegung
DE60238392D1 (de) Verfahren und vorrichtung zur schätzung der dopplerspreizung
AT5911U3 (de) Verfahren zur kontaktfreien messung eines querprofils bzw. abstands von schienen eines gleises
ATE421791T1 (de) Verfahren und vorrichtung zur arcerkennung in einem plasmaprozess
DE602004023824D1 (de) Verfahren zum direkten nachweis einzelner methotrexatmetabolite
ATE553142T1 (de) Verfahren zur fraktionierung/konzentration zur verringerung der polydispersität von polyetherimiden
ATE397098T1 (de) Verfahren zur diagnostik von eierstock endometriose
EP4383874A4 (de) Verfahren und vorrichtung zum senden und empfangen eines signals und kommunikationssystem
EP4503493A4 (de) Verfahren und vorrichtung zur bestimmung der beziehung zwischen ptrs-ports und dmrs-ports, medium und produkt
ATE335186T1 (de) Verfahren und vorrichtung zur erfassung von auf einem fördermittel bewegten objekten mittels eines optoelektronischen sensors
ATE328121T1 (de) Nachweisbares kationisches flockungsmittel sowie verfahren zur verwendung desselben in industriellen lebensmittelprozessen
ATE356992T1 (de) Verfahren und vorrichtung zur messung des aldehyd-gehalts in einem polyester-polymer
ATE540499T1 (de) Verfahren und vorrichtung zur signalintegritätsverifikation
ATE422334T1 (de) System und verfahren zur gewebeanalyse
EP4568358A4 (de) Verfahren zur bestimmung von aufwecksignalinformationen, endgerät und netzwerkseitige vorrichtung
DE50310921D1 (de) Verfahren und Vorrichtung zur Übertragung von Information und Fehlererkennung in einem ringförmigen Netzwerk
DE60202656D1 (de) Verfahren und Vorrichtung zur Ermittlung von Frequenzdifferenzen, und Phasenregelkreis
EP4429359A4 (de) Verfahren und vorrichtung zur bestimmung verfügbarer zeitschlitze und endgerät

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties