ATE438858T1 - Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimieren - Google Patents
Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimierenInfo
- Publication number
- ATE438858T1 ATE438858T1 AT04735027T AT04735027T ATE438858T1 AT E438858 T1 ATE438858 T1 AT E438858T1 AT 04735027 T AT04735027 T AT 04735027T AT 04735027 T AT04735027 T AT 04735027T AT E438858 T1 ATE438858 T1 AT E438858T1
- Authority
- AT
- Austria
- Prior art keywords
- target protein
- soluble variant
- express
- filter
- cell colonies
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 108010052285 Membrane Proteins Proteins 0.000 abstract 1
- 102000018697 Membrane Proteins Human genes 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000006166 lysate Substances 0.000 abstract 1
- 239000011148 porous material Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Threshing Machine Elements (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0315525.6A GB0315525D0 (en) | 2003-07-02 | 2003-07-02 | A method of screening |
| PCT/GB2004/002271 WO2005003784A1 (en) | 2003-07-02 | 2004-05-27 | A method of screening for cell colonies that express a soluble variant of a target protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE438858T1 true ATE438858T1 (de) | 2009-08-15 |
Family
ID=27676553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04735027T ATE438858T1 (de) | 2003-07-02 | 2004-05-27 | Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimieren |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7718381B2 (de) |
| EP (1) | EP1639373B1 (de) |
| AT (1) | ATE438858T1 (de) |
| AU (1) | AU2004254096B2 (de) |
| CA (1) | CA2531026C (de) |
| DE (1) | DE602004022414D1 (de) |
| DK (1) | DK1639373T3 (de) |
| ES (1) | ES2331311T3 (de) |
| GB (1) | GB0315525D0 (de) |
| WO (1) | WO2005003784A1 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0419628D0 (en) * | 2004-09-03 | 2004-10-06 | European Molecular Biology Lab Embl | Method for determining protein solubility |
| WO2012135535A1 (en) * | 2011-03-31 | 2012-10-04 | Los Alamos National Security, Llc | Method of identifying soluble proteins and soluble protein complexes |
| US9523693B2 (en) | 2011-04-18 | 2016-12-20 | Biotarget Engagement Interest Group Ab | Methods for determining ligand binding to a target protein using a thermal shift assay |
| GB201106548D0 (en) | 2011-04-18 | 2011-06-01 | Evitraproteoma Ab | A method for determining ligand binding to a target protein using a thermal shift assahy |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2152214A (en) | 1983-11-08 | 1985-07-31 | Univ Surrey | Detection of enterotoxigenic E. coli |
| DE4237113B4 (de) * | 1992-11-03 | 2006-10-12 | "Iba Gmbh" | Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins |
| US20020127587A1 (en) * | 2001-02-13 | 2002-09-12 | Domenica Simms | Methods and compositions for isolation of biological macromolecules |
-
2003
- 2003-07-02 GB GBGB0315525.6A patent/GB0315525D0/en not_active Ceased
-
2004
- 2004-05-27 DK DK04735027T patent/DK1639373T3/da active
- 2004-05-27 EP EP04735027A patent/EP1639373B1/de not_active Expired - Lifetime
- 2004-05-27 AU AU2004254096A patent/AU2004254096B2/en not_active Ceased
- 2004-05-27 DE DE602004022414T patent/DE602004022414D1/de not_active Expired - Lifetime
- 2004-05-27 CA CA2531026A patent/CA2531026C/en not_active Expired - Lifetime
- 2004-05-27 US US10/562,734 patent/US7718381B2/en not_active Expired - Lifetime
- 2004-05-27 ES ES04735027T patent/ES2331311T3/es not_active Expired - Lifetime
- 2004-05-27 WO PCT/GB2004/002271 patent/WO2005003784A1/en not_active Ceased
- 2004-05-27 AT AT04735027T patent/ATE438858T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2531026A1 (en) | 2005-01-13 |
| AU2004254096A1 (en) | 2005-01-13 |
| WO2005003784A1 (en) | 2005-01-13 |
| GB0315525D0 (en) | 2003-08-06 |
| DK1639373T3 (da) | 2009-12-07 |
| EP1639373A1 (de) | 2006-03-29 |
| US7718381B2 (en) | 2010-05-18 |
| EP1639373B1 (de) | 2009-08-05 |
| AU2004254096B2 (en) | 2009-06-11 |
| DE602004022414D1 (de) | 2009-09-17 |
| ES2331311T3 (es) | 2009-12-29 |
| US20070087325A1 (en) | 2007-04-19 |
| CA2531026C (en) | 2010-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69943048D1 (de) | Methode zum nachweis biologischer faktoren in der epidermis | |
| WO2006047481A3 (en) | Enhanced amplifiability of minute fixative-treated tissue samples, minute stained cytology samples, and other minute sources of dna | |
| WO2015128272A3 (en) | Photo-selective method for biological sample analysis field | |
| WO2007027905A3 (en) | Human renal stem cells | |
| WO2007061943A8 (en) | Portable preparation, analysis, and detection apparatus for nucleic acid processing | |
| WO2013157917A3 (en) | A biosensor and preparation method thereof | |
| DE60300339D1 (de) | Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben | |
| ATE307902T1 (de) | Methoden und testsätze zur probennahme und aufbewahrung von biologischen proben | |
| MX2011003982A (es) | Métodos para la separación, caracterización y/o identificación de microorganismos usando espectroscopia. | |
| ATE356884T1 (de) | Methode zur detektion von pathogenen organismen | |
| MX2021012677A (es) | Identificacion de proteinas de celulas huesped. | |
| DE59912716D1 (de) | Verfahren zur bestimmung der immunabwehraktivität des blutes sowie testkit hierfür | |
| ATE461443T1 (de) | Verfahren zur bestimmung der viabilität von zellen in zellkulturen | |
| EA201990294A1 (ru) | Способ и система для микробиальной фармакогеномики | |
| WO2016127077A8 (en) | Systems and methods for detection and analysis of biological species | |
| RU2009117464A (ru) | Способ определения фрагментации днк в микроорганизмах | |
| DE60108337D1 (de) | Methode und testsatz zur detektion von schistosomiasis mittels polymerasekettenreaktion | |
| ATE438858T1 (de) | Methode zum auffinden von zellkolonien die eine lösliche variante eines zielproteins exprimieren | |
| DE602005018742D1 (de) | Verfahren zur bestimmung der dna-fragmentierung in spermienzellen von tieren | |
| DE50105263D1 (de) | Verfahren zur analyse von proteinen | |
| WO2021026025A3 (en) | Method of testing for specific organisms in an individual | |
| ATE345397T1 (de) | Verfahren zur isolierung von bakterien aus biologischen proben | |
| WO2007095359A3 (en) | Assay for distinguishing live and dead cells | |
| WO2014014696A3 (en) | Sample dependent selection of parameters for use in electrokinetic treatment of the sample | |
| DE602004019180D1 (de) | Methode zur Identifizierung von unbekannten Proteinen mittels zwei-dimensionaler Auftrennung und Verwendung von Markern |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |