ATE445704T1 - Expressionsvektor für tierische zellen - Google Patents
Expressionsvektor für tierische zellenInfo
- Publication number
- ATE445704T1 ATE445704T1 AT01957013T AT01957013T ATE445704T1 AT E445704 T1 ATE445704 T1 AT E445704T1 AT 01957013 T AT01957013 T AT 01957013T AT 01957013 T AT01957013 T AT 01957013T AT E445704 T1 ATE445704 T1 AT E445704T1
- Authority
- AT
- Austria
- Prior art keywords
- vector
- expression vector
- present
- animal cells
- expression
- Prior art date
Links
- 239000013604 expression vector Substances 0.000 title abstract 4
- 241001465754 Metazoa Species 0.000 title abstract 2
- 239000013598 vector Substances 0.000 abstract 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 2
- 108010052343 Gastrins Proteins 0.000 abstract 1
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 abstract 1
- 210000004102 animal cell Anatomy 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000005030 transcription termination Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4717—Plasma globulins, lactoglobulin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/595—Gastrins; Cholecystokinins [CCK]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Endocrinology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2000-0043996A KR100408844B1 (ko) | 2000-07-29 | 2000-07-29 | 동물세포 발현벡터 |
| PCT/KR2001/001285 WO2002014525A2 (en) | 2000-07-29 | 2001-07-27 | Expression vector using for animal cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE445704T1 true ATE445704T1 (de) | 2009-10-15 |
Family
ID=19680759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01957013T ATE445704T1 (de) | 2000-07-29 | 2001-07-27 | Expressionsvektor für tierische zellen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7422874B2 (de) |
| EP (1) | EP1392835B1 (de) |
| JP (1) | JP4138479B2 (de) |
| KR (1) | KR100408844B1 (de) |
| CN (1) | CN1262663C (de) |
| AT (1) | ATE445704T1 (de) |
| AU (1) | AU2001278797A1 (de) |
| DE (1) | DE60140218D1 (de) |
| ES (1) | ES2333774T3 (de) |
| WO (1) | WO2002014525A2 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2435972C (en) * | 2001-01-26 | 2011-09-13 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
| PL210412B1 (pl) | 2002-05-24 | 2012-01-31 | Schering Corp | Izolowane przeciwciało anty-IGFR lub jego fragment, kompozycja farmaceutyczna, zastosowanie, kwas nukleinowy, zrekombinowany wektor, komórka gospodarza, kompleks, polipeptyd i sposób jego wytwarzania |
| AU2003263161A1 (en) * | 2002-09-20 | 2004-04-08 | Novo Nordisk A/S | Method for making recombinant proteins |
| US6933136B2 (en) | 2002-09-20 | 2005-08-23 | Novo Nordisk A/S | Method for making recombinant proteins |
| KR100755931B1 (ko) * | 2003-06-11 | 2007-09-06 | (주)두비엘 | 고분자형 만노스 결합형 렉틴의 대량 생산방법 및 상기방법으로 제조된 만노스 결합형 렉틴 재조합 단백질의제제화 |
| US7135338B2 (en) * | 2003-06-11 | 2006-11-14 | Dobeel Corporation | Methods for overexpression of high molecular weight form of mannose binding lectin (MBL) and a specific formulation for active treatment for systemic infection with microorganism |
| CA2535836C (en) | 2003-10-24 | 2014-02-04 | Nicolas Mermod | High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of mar sequences |
| WO2005047512A2 (en) * | 2003-11-12 | 2005-05-26 | Shering Corporation | Plasmid system for multigene expression |
| AR046639A1 (es) | 2003-11-21 | 2005-12-14 | Schering Corp | Combinaciones terapeuticas de anticuerpo anti- igfr1 |
| WO2006046786A1 (en) * | 2004-10-28 | 2006-05-04 | Dobeel Co., Ltd. | Method for the mass production of multimeric mannose binding lectin |
| EP1859046B1 (de) * | 2005-03-04 | 2010-06-16 | Celltrion, Inc. | Expressionsvektor für tierische zelle mit wenigstens einer kopie von mar-dna-sequenzen am 3'-ende des transkriptionsterminationsbereichs eines gens sowie verfahren zur expression eines fremdgens unter verwendung des vektors |
| KR100779761B1 (ko) * | 2005-10-28 | 2007-11-30 | 대한민국 | 인슐린 억제 및 사람 ide 유전자로 형질전환된 동물 및그 제조방법 |
| KR100836745B1 (ko) * | 2007-01-31 | 2008-06-10 | (주)두비엘 | Hbv 백신 및 그의 제조 방법 |
| KR101258949B1 (ko) | 2007-06-15 | 2013-04-30 | 재단법인 목암생명공학연구소 | 활성형 재조합 혈액응고 9인자의 대량생산 방법 |
| CN101348786B (zh) * | 2007-07-20 | 2011-05-11 | 谭孟群 | 一种人β珠蛋白基因及其重组腺相关病毒载体 |
| US20110195451A1 (en) * | 2008-06-10 | 2011-08-11 | Isis Innovation Limited | Protein Expression |
| KR101054362B1 (ko) | 2008-07-03 | 2011-08-05 | 재단법인 목암생명공학연구소 | 재조합 단백질의 푸코스 함량을 감소시키는 방법 |
| US8137933B2 (en) | 2008-11-12 | 2012-03-20 | Schering Corporation | Mammalian expression vector pUHAB |
| US20120064631A1 (en) * | 2009-03-27 | 2012-03-15 | Hiroshima University | Method for amplification and high-level expression of target gene in mammalian cell, and kit for achieving the method |
| KR20120099376A (ko) * | 2009-09-18 | 2012-09-10 | 셀렉시스 에스. 에이. | 강화된 전이 유전자 발현과 공정 방법 및 그 산물 |
| CN102639699A (zh) * | 2009-10-01 | 2012-08-15 | Toto株式会社 | Dna构建体以及用其制备重组cho细胞的方法 |
| US20130034875A1 (en) * | 2009-10-01 | 2013-02-07 | Toto Ltd. | Dna construct, and process for production of recombinant cho cell using same |
| BR112013008459A2 (pt) | 2010-10-08 | 2016-06-28 | Cadila Healthcare Ltd | ''vetor de expressão,célula hospedeira,processo para a produção de proteinas e de peptideos e dos variantes dos mesmos'' |
| JP5931174B2 (ja) * | 2011-03-30 | 2016-06-08 | パンジェン バイオテク インコーポレイテッド | 動物細胞発現ベクター |
| JP5972866B2 (ja) | 2011-03-31 | 2016-08-17 | クニミネ工業株式会社 | タンパク質結晶化条件探索剤及びタンパク質結晶化条件探索方法 |
| KR101420274B1 (ko) * | 2011-06-13 | 2014-07-21 | 주식회사 종근당 | Csp-b 5'sar 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 |
| CA2849318C (en) | 2011-09-22 | 2019-11-12 | Amgen Inc. | Cd27l antigen binding proteins |
| CN104011075B (zh) * | 2011-12-22 | 2017-06-06 | 弗·哈夫曼-拉罗切有限公司 | 表达载体元件组合、新的生产用细胞产生方法及其在重组产生多肽中的用途 |
| SG11201403223PA (en) | 2011-12-22 | 2014-07-30 | Hoffmann La Roche | Expression vector organization, novel production cell generation methods and their use for the recombinant production of polypeptides |
| UY34813A (es) | 2012-05-18 | 2013-11-29 | Amgen Inc | Proteínas de unión a antígeno dirigidas contra el receptor st2 |
| UY35148A (es) | 2012-11-21 | 2014-05-30 | Amgen Inc | Immunoglobulinas heterodiméricas |
| US9546203B2 (en) | 2013-03-14 | 2017-01-17 | Amgen Inc. | Aglycosylated Fc-containing polypeptides with cysteine substitutions |
| WO2014144817A2 (en) | 2013-03-15 | 2014-09-18 | Amgen Inc. | Inhibitory polypeptides specific to wnt inhibitors |
| CN103194455B (zh) * | 2013-04-08 | 2014-10-15 | 山东省农业科学院畜牧兽医研究所 | 高效表达的猪粒细胞巨噬细胞集落刺激因子基因及其表达蛋白的用途 |
| IL317484A (en) | 2013-05-30 | 2025-02-01 | Kiniksa Pharmaceuticals Ltd | Oncostatin M receptor antigen-binding proteins |
| CA2923145C (en) | 2013-09-05 | 2025-09-16 | Amgen Inc. | MOLECULES CONTAINING FCS AND EXHIBITING PREDICTABLE, UNIFORM AND REPRODUCIBLE GLYCOFORM PROFILES |
| AU2014317961B2 (en) | 2013-09-05 | 2020-07-30 | Murdoch University | Antisense-induced exon2 inclusion in acid alpha-glucosidase |
| KR101591823B1 (ko) | 2013-12-27 | 2016-02-04 | 재단법인 목암생명공학연구소 | 증가된 유전자 발현능을 갖는 발현벡터 |
| EA201792250A1 (ru) | 2015-04-10 | 2018-05-31 | Эмджен Инк. | Мутеины интерлейкина-2 для роста регуляторных т-клеток |
| WO2016164937A2 (en) | 2015-04-10 | 2016-10-13 | Amgen Inc. | Interleukin-2 muteins for the expansion of t-regulatory cells |
| CA2980189A1 (en) | 2015-04-24 | 2016-10-27 | Genentech, Inc. | Multispecific antigen-binding proteins |
| MX2018003445A (es) | 2015-09-22 | 2018-08-01 | Genentech Inc | Expresion de proteinas que contienen fc. |
| EP4524161A3 (de) | 2015-10-08 | 2025-06-04 | Zymeworks BC Inc. | Antigenbindende polypeptidkonstrukte mit kappa- und lambda-leichtketten und verwendungen davon |
| SG11201802773RA (en) | 2015-11-02 | 2018-05-30 | Genentech Inc | Methods of making fucosylated and afucosylated forms of a protein |
| BR112018071477A2 (pt) | 2016-04-18 | 2019-02-19 | Sarepta Therapeutics, Inc. | oligômeros antisenso e métodos de utilização dos mesmos para tratamento de doenças associadas com o gene da alfa-glicosidase ácida |
| WO2018150345A1 (en) * | 2017-02-16 | 2018-08-23 | De Vita Bio Life Sciences | An expression vector |
| US11965019B2 (en) | 2017-06-30 | 2024-04-23 | Zymeworks Bc Inc. | Stabilized chimeric Fabs |
| CN110343718A (zh) * | 2018-04-03 | 2019-10-18 | 新乡医学院 | 一种高效稳定的细胞表达载体、表达系统及其制备方法、应用 |
| AU2020328038B2 (en) | 2019-08-13 | 2025-10-09 | Amgen Inc. | Interleukin-2 muteins for the expansion of T-regulatory cells |
| MX2022007712A (es) | 2019-12-17 | 2022-09-26 | Amgen Inc | Agonista doble de interleucina-2/receptor de tnf para uso en terapia. |
| AU2024307352A1 (en) | 2023-06-27 | 2025-12-11 | Amgen Inc. | Charge pair mutations to enable correct heavy-light chain pairing |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4956288A (en) * | 1988-04-22 | 1990-09-11 | Biogen, Inc. | Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA |
| US5108901A (en) * | 1988-09-02 | 1992-04-28 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
| EP0663921B1 (de) | 1992-10-05 | 2002-05-08 | North Carolina State University | Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen |
| US5738985A (en) * | 1993-04-02 | 1998-04-14 | Ribogene, Inc. | Method for selective inactivation of viral replication |
| US5773695A (en) | 1996-01-26 | 1998-06-30 | North Carolina State University | Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells |
| WO1997046687A1 (en) * | 1996-06-06 | 1997-12-11 | Novartis Ag | Vectors comprising sar elements |
| EP0914454B1 (de) | 1996-06-11 | 2005-03-09 | Pioneer Hi-Bred International, Inc. | Ein synthetischer, pflanzlicher kernpromotor und stromaufwaerts gelegenes, regulatorisches element |
| US6245974B1 (en) | 1997-08-06 | 2001-06-12 | North Carolina State University | Matrix attachment regions |
| KR100313559B1 (ko) * | 1998-07-22 | 2002-03-08 | 허영섭 | 수용성 TGFβ 수용체를 발현하는 재조합 세포주 및 전기세포주를 이용한 암치료 방법 |
| EP1117815A1 (de) * | 1998-09-29 | 2001-07-25 | Pioneer Hi-Bred International Inc. | Mar/sar elemente, die ein durch rsyn7 reguliertes konstrukt flankieren |
| CA2435972C (en) * | 2001-01-26 | 2011-09-13 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
-
2000
- 2000-07-29 KR KR10-2000-0043996A patent/KR100408844B1/ko not_active Expired - Lifetime
-
2001
- 2001-07-27 CN CNB018135579A patent/CN1262663C/zh not_active Expired - Lifetime
- 2001-07-27 JP JP2002519651A patent/JP4138479B2/ja not_active Expired - Lifetime
- 2001-07-27 EP EP01957013A patent/EP1392835B1/de not_active Expired - Lifetime
- 2001-07-27 AT AT01957013T patent/ATE445704T1/de not_active IP Right Cessation
- 2001-07-27 DE DE60140218T patent/DE60140218D1/de not_active Expired - Lifetime
- 2001-07-27 ES ES01957013T patent/ES2333774T3/es not_active Expired - Lifetime
- 2001-07-27 US US10/343,303 patent/US7422874B2/en not_active Expired - Lifetime
- 2001-07-27 WO PCT/KR2001/001285 patent/WO2002014525A2/en not_active Ceased
- 2001-07-27 AU AU2001278797A patent/AU2001278797A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004506428A (ja) | 2004-03-04 |
| DE60140218D1 (de) | 2009-11-26 |
| US20040038394A1 (en) | 2004-02-26 |
| WO2002014525A2 (en) | 2002-02-21 |
| CN1444657A (zh) | 2003-09-24 |
| EP1392835A2 (de) | 2004-03-03 |
| KR100408844B1 (ko) | 2003-12-06 |
| WO2002014525A3 (en) | 2003-11-20 |
| EP1392835B1 (de) | 2009-10-14 |
| CN1262663C (zh) | 2006-07-05 |
| ES2333774T3 (es) | 2010-03-01 |
| US7422874B2 (en) | 2008-09-09 |
| JP4138479B2 (ja) | 2008-08-27 |
| EP1392835A4 (de) | 2006-04-19 |
| AU2001278797A1 (en) | 2002-02-25 |
| KR20020010327A (ko) | 2002-02-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |