ATE446370T1 - Öl, das brassicastanol enthält - Google Patents
Öl, das brassicastanol enthältInfo
- Publication number
- ATE446370T1 ATE446370T1 AT00922076T AT00922076T ATE446370T1 AT E446370 T1 ATE446370 T1 AT E446370T1 AT 00922076 T AT00922076 T AT 00922076T AT 00922076 T AT00922076 T AT 00922076T AT E446370 T1 ATE446370 T1 AT E446370T1
- Authority
- AT
- Austria
- Prior art keywords
- seeds
- transgenic plants
- brassicastanol
- sitostanol
- dna sequences
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
- Lubricants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pyrane Compounds (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12899599P | 1999-04-12 | 1999-04-12 | |
| PCT/US2000/009696 WO2000061771A2 (en) | 1999-04-12 | 2000-04-12 | Transgenic plants containing altered levels of sterol compounds and tocopherols |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE446370T1 true ATE446370T1 (de) | 2009-11-15 |
Family
ID=22437998
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00922076T ATE446370T1 (de) | 1999-04-12 | 2000-04-12 | Öl, das brassicastanol enthält |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US7709666B2 (de) |
| EP (1) | EP1169462B1 (de) |
| CN (1) | CN1360637A (de) |
| AT (1) | ATE446370T1 (de) |
| AU (1) | AU4231600A (de) |
| BR (1) | BR0010597A (de) |
| CA (1) | CA2372120A1 (de) |
| DE (1) | DE60043189D1 (de) |
| HK (1) | HK1041608A1 (de) |
| MX (1) | MXPA01010304A (de) |
| WO (1) | WO2000061771A2 (de) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| EP1559788A1 (de) | 1997-04-09 | 2005-08-03 | Danisco A/S | Verwendung von Lipase zur Verbesserung von Teigen und Backwaren |
| MXPA01010488A (es) | 1999-04-15 | 2003-07-21 | Calgene Llc | Secuencias de acidos nucleicos para proteinas que intervienen en sintesis isoprenoide. |
| WO2000068393A1 (en) | 1999-05-07 | 2000-11-16 | Pioneer Hi-Bred International, Inc. | Phytyl/prenyltransferase nucleic acids, polypeptides and uses thereof |
| GB9925453D0 (en) * | 1999-10-27 | 1999-12-29 | Univ Bristol | Increasing isoprenoid biosynthesis |
| BR0014934A (pt) * | 1999-10-27 | 2002-06-18 | Unilever Nv | Usos de um gene que expressa uma hmg-redutase sem retro-inibição e de um gene heterólogo que expressa uma hmg-redutase sem retro-inibição truncada, método de obtenção de sementes, planta, tecido de planta, sementes, método de obtenção de óleo, e, produto alimentìcio |
| DK1121928T3 (da) * | 2000-01-31 | 2008-03-17 | Haerting S A | Sammensætninger indeholdende phytosterol- og policosanolestere af fedtsyrer til reduktion af niveauet af blodcholesterol og -triglycerider |
| CA2406076A1 (en) * | 2000-04-14 | 2001-10-25 | Unilever Plc | Process for modifying plants |
| US6872815B1 (en) | 2000-10-14 | 2005-03-29 | Calgene Llc | Nucleic acid sequences to proteins involved in tocopherol synthesis |
| ATE447017T1 (de) | 2000-08-07 | 2009-11-15 | Monsanto Technology Llc | Am methyl-d-erythriol phosphat reaktionsweg beteiligte gene |
| US20040019192A1 (en) * | 2000-11-24 | 2004-01-29 | Mark Harker | Process for modifying plants |
| US6822142B2 (en) | 2001-01-05 | 2004-11-23 | Monsanto Company | Transgenic plants containing altered levels of steroid compounds |
| DE10111676A1 (de) * | 2001-03-09 | 2002-09-12 | Sungene Gmbh & Co Kgaa | Erhöhung des Vitamin-E-Gehalts in Organismen durch Erhöhung der Tyrosinaminotransferase-Aktivität |
| CA2443865C (en) * | 2001-05-09 | 2012-02-07 | Monsanto Technology Llc | Tyra genes and uses thereof |
| EP1950305A1 (de) | 2001-05-09 | 2008-07-30 | Monsanto Technology, LLC | Tyr-A-Gene und ihre Verwendung |
| US7161061B2 (en) | 2001-05-09 | 2007-01-09 | Monsanto Technology Llc | Metabolite transporters |
| AU2002342260A1 (en) * | 2001-05-11 | 2002-11-25 | Bayer Aktiengesellschaft | Regulation of human steroid 5-alpha reductase-like protein |
| ES2284897T3 (es) | 2001-05-18 | 2007-11-16 | Danisco A/S | Procedimiento para la preparacion de una masa con una enzima. |
| CA2448881A1 (en) | 2001-06-04 | 2002-12-12 | Basf Plant Science Gmbh | Sugar and lipid metabolism regulators in plants ii |
| WO2003016482A2 (en) | 2001-08-17 | 2003-02-27 | Monsanto Technology Llc | Methyltransferase genes and uses thereof |
| AR036962A1 (es) | 2001-10-25 | 2004-10-13 | Monsanto Technology Llc | Metiltransferasas aromaticas y usos de las mismas |
| BR0308740A (pt) | 2002-03-19 | 2007-01-09 | Monsanto Technology Llc | ácidos nucléicos e polipeptìdeos de homogentisado prenil transferase ("hpt"), e empregos destes |
| EP2216405A1 (de) | 2002-05-03 | 2010-08-11 | Monsanto Technology LLC | Saatspezifische USP-Promotoren zur Expression von Genen in Pflanzen |
| BR0313270A (pt) * | 2002-08-05 | 2005-08-02 | Monsanto Technology Llc | Genes relacionados com a biosìntese de tocoferol e usos dos mesmos |
| FR2844142B1 (fr) | 2002-09-11 | 2007-08-17 | Bayer Cropscience Sa | Plantes transformees a biosynthese de prenylquinones amelioree |
| MXPA05007653A (es) | 2003-01-17 | 2005-09-30 | Danisco | Metodo. |
| EP1648238A1 (de) * | 2003-07-29 | 2006-04-26 | Unilever N.V. | Nahrungsmittel enthaltend phytosterole |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| CN102533440B (zh) | 2004-07-16 | 2017-09-19 | 杜邦营养生物科学有限公司 | 用酶使油脱胶的方法 |
| CA2603151A1 (en) * | 2005-03-30 | 2006-10-05 | Jitao Zou | Identification of a sterol acyltransferase gene |
| AR053269A1 (es) * | 2005-05-16 | 2007-04-25 | Monsanto Technology Llc | Plantas y semillas de maiz con mejoramiento de asparagina y proteina |
| EP1928908A2 (de) | 2005-09-20 | 2008-06-11 | BASF Plant Science GmbH | Für konstitutive triple-response1-like polypeptide codierende nukleinsäuremoleküle und verfahren zu deren anwendung |
| US8420890B2 (en) | 2006-03-28 | 2013-04-16 | Cornell Research Foundation, Inc. | Use of NAP gene to manipulate leaf senescence in plants |
| US20090004715A1 (en) | 2007-06-01 | 2009-01-01 | Solazyme, Inc. | Glycerol Feedstock Utilization for Oil-Based Fuel Manufacturing |
| MY182693A (en) | 2008-11-28 | 2021-01-29 | Terravia Holdings Inc | Production of tailored oils in heterotrophic microorganisms |
| CN102316753B (zh) | 2009-01-16 | 2016-06-29 | 杜邦营养生物科学有限公司 | 由谷物或谷物副流酶促生成功能性脂质 |
| BRPI1012562B1 (pt) | 2009-03-31 | 2020-10-27 | Dupont Nutrition Biosciences Aps | método para reduzir a cor e/ou sabor desagradável e/ou desenvolvimento de mau cheiro em um farelo de cereal solubilizado |
| WO2010122405A2 (en) * | 2009-04-23 | 2010-10-28 | Avesthagen Limited | A method for over expression of chemical constituents in plants |
| WO2011132197A1 (en) | 2010-04-20 | 2011-10-27 | Chetan Balar | An efficient compound for increasing reproductive growth in crop plants, vegetable plants, spices plants, flower and gardening plants thereof |
| SG10201504187YA (en) | 2010-05-28 | 2015-06-29 | Solazyme Inc | Tailored oils produced from recombinant heterotrophic microorganisms |
| CN108823254A (zh) | 2010-11-03 | 2018-11-16 | 柯碧恩生物技术公司 | 具有降低倾点的微生物油、从其中产生的介电流体、以及相关方法 |
| MX344012B (es) | 2011-02-02 | 2016-12-02 | Terravia Holdings Inc | Aceites adaptados producidos a partir de microorganismos oleaginosos recombinantes. |
| US8846352B2 (en) | 2011-05-06 | 2014-09-30 | Solazyme, Inc. | Genetically engineered microorganisms that metabolize xylose |
| US9719114B2 (en) | 2012-04-18 | 2017-08-01 | Terravia Holdings, Inc. | Tailored oils |
| KR20150001830A (ko) | 2012-04-18 | 2015-01-06 | 솔라짐, 인코포레이티드 | 맞춤 오일 |
| US9567615B2 (en) | 2013-01-29 | 2017-02-14 | Terravia Holdings, Inc. | Variant thioesterases and methods of use |
| US9816079B2 (en) | 2013-01-29 | 2017-11-14 | Terravia Holdings, Inc. | Variant thioesterases and methods of use |
| US9783836B2 (en) | 2013-03-15 | 2017-10-10 | Terravia Holdings, Inc. | Thioesterases and cells for production of tailored oils |
| US9290749B2 (en) | 2013-03-15 | 2016-03-22 | Solazyme, Inc. | Thioesterases and cells for production of tailored oils |
| SG11201602638SA (en) | 2013-10-04 | 2016-05-30 | Solazyme Inc | Tailored oils |
| CN106574255A (zh) | 2014-07-10 | 2017-04-19 | 泰拉瑞亚控股公司 | 酮脂酰acp合酶基因及其用途 |
| EP3172320B1 (de) | 2014-07-24 | 2019-11-20 | Corbion Biotech, Inc. | Variante thioesterasen und verfahren zur verwendung |
| CN107208103A (zh) | 2014-09-18 | 2017-09-26 | 泰拉瑞亚控股公司 | 酰基‑acp硫酯酶及其突变体 |
| CN113174395B (zh) * | 2021-04-30 | 2022-06-24 | 中国烟草总公司郑州烟草研究院 | 与烟气苯酚释放量相关的突变基因dhq-sdh1 |
| WO2022240690A1 (en) * | 2021-05-14 | 2022-11-17 | Canisus College | Methods and compositions for improving carbon accumulation in plants |
| CN118755867B (zh) * | 2024-07-22 | 2025-02-07 | 广东省农业科学院作物研究所 | 一种检测甜玉米籽粒gamma-生育三烯酚含量的Zmhppd1基因及其应用 |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4588717A (en) * | 1984-06-13 | 1986-05-13 | David C. Mitchell Medical Research Institute | Compounds and vitamin supplements and methods for making same |
| US5420034A (en) | 1986-07-31 | 1995-05-30 | Calgene, Inc. | Seed-specific transcriptional regulation |
| US5753475A (en) | 1985-01-17 | 1998-05-19 | Calgene, Inc. | Methods and compositions for regulated transcription and expression of heterologous genes |
| NZ221259A (en) | 1986-07-31 | 1990-05-28 | Calgene Inc | Seed specific transcriptional regulation |
| US5693507A (en) | 1988-09-26 | 1997-12-02 | Auburn University | Genetic engineering of plant chloroplasts |
| CA1340449C (en) | 1988-11-14 | 1999-03-16 | Susan Long | Expression of dna sequences derived from nocardioform microorganisms |
| US5530185A (en) | 1989-07-19 | 1996-06-25 | Calgene, Inc. | Use of ovary-tissue transcriptional factors |
| US5270041A (en) * | 1989-07-21 | 1993-12-14 | Marigen S.A. | Sterols, their fatty acid esters and glucosides; processes for their preparation; spontaneously dispersible agents containing these compounds, and their use for treatment of tumors |
| US5451513A (en) | 1990-05-01 | 1995-09-19 | The State University of New Jersey Rutgers | Method for stably transforming plastids of multicellular plants |
| US5306862A (en) | 1990-10-12 | 1994-04-26 | Amoco Corporation | Method and composition for increasing sterol accumulation in higher plants |
| HK1001951A1 (en) * | 1991-05-03 | 1998-07-24 | Raisio Benecol Ltd. | A substance for lowering high cholesterol level in serum and a method for preparing the same |
| EP0531639B1 (de) | 1991-07-18 | 1999-09-01 | F. Hoffmann-La Roche Ag | Tocopherol-Cyclase |
| US5558862A (en) | 1991-09-23 | 1996-09-24 | Monsanto Company | Method of controlling insects |
| US5763245A (en) | 1991-09-23 | 1998-06-09 | Monsanto Company | Method of controlling insects |
| US5518908A (en) | 1991-09-23 | 1996-05-21 | Monsanto Company | Method of controlling insects |
| FR2687284B1 (fr) * | 1992-02-14 | 1995-06-23 | Verneuil Rech | Plante portant des genes codant pour des enzymes de la voie de biosynthese des phytosterols, et procede d'obtention. |
| US5244887A (en) * | 1992-02-14 | 1993-09-14 | Straub Carl D | Stanols to reduce cholesterol absorption from foods and methods of preparation and use thereof |
| US5576198A (en) | 1993-12-14 | 1996-11-19 | Calgene, Inc. | Controlled expression of transgenic constructs in plant plastids |
| US5545818A (en) | 1994-03-11 | 1996-08-13 | Calgene Inc. | Expression of Bacillus thuringiensis cry proteins in plant plastids |
| US5545817A (en) | 1994-03-11 | 1996-08-13 | Calgene, Inc. | Enhanced expression in a plant plastid |
| US5530191A (en) | 1994-03-24 | 1996-06-25 | Rutgers, The State University Of New Jersey | Method for producing cytoplasmic male sterility in plants and use thereof in production of hybrid seed |
| EP0828434B1 (de) | 1995-06-01 | 2000-02-09 | Unilever N.V. | Fettenthaltende nahrungsmittelprodukte |
| US5811636A (en) | 1995-09-22 | 1998-09-22 | The United States Of America As Represented By The Secretary Of Agriculture | Apomixis for producing true-breeding plant progenies |
| US6087563A (en) | 1996-01-29 | 2000-07-11 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Cloned arabidopsis p-hydroxyphenyl pyruvic acid dioxygenase DNA |
| US6143950A (en) * | 1996-04-18 | 2000-11-07 | The Salk Institute For Biological Studies | Plant steroid 5α reductase, DET2 |
| WO1997042830A1 (en) | 1996-05-10 | 1997-11-20 | Unilever N.V. | Liquid fatty component containing composition |
| WO1997048793A1 (en) * | 1996-06-21 | 1997-12-24 | The General Hospital Corporation | Plant sterol reductases and uses thereof |
| FI107015B (fi) * | 1996-08-09 | 2001-05-31 | Raisio Benecol Oy | Kasvistanolirasvahappoestereiden seos ja sen käyttö sekä elintarvike |
| US6429356B1 (en) * | 1996-08-09 | 2002-08-06 | Calgene Llc | Methods for producing carotenoid compounds, and specialty oils in plant seeds |
| BR9713462A (pt) * | 1996-08-09 | 2000-03-28 | Calgene Inc | Métodos para produção de compostos carotenóides e óleos especiais em sementes de plantas. |
| US6139872A (en) | 1996-08-14 | 2000-10-31 | Henkel Corporation | Method of producing a vitamin product |
| US6498239B1 (en) * | 1996-10-21 | 2002-12-24 | Gesellschaft fur Erwerb und Verwertung von Schutzrechten—GVS mbH | Sterol glycosyl transferases |
| US5998396A (en) * | 1996-11-05 | 1999-12-07 | Riken Vitamin Co., Ltd. | Oil solubilized solutions and foods containing phytosterols and process for their production |
| EP0958370A1 (de) * | 1996-12-26 | 1999-11-24 | Monsanto Company | Transgene pflanzen mit veränderten sterol-biosynthesewegen |
| US6642434B1 (en) * | 1997-07-25 | 2003-11-04 | University Of Community College System Of Nevada | Transgenic plants with γ-tocopherol methyltransferase |
| MXPA00012100A (es) * | 1998-06-05 | 2003-04-22 | Calgene Llc | Secuencias de acidos nucleicos relacionados con acil coenzima a: colesterol aciltransferasa. |
| AU5250399A (en) * | 1998-08-03 | 2000-02-28 | Arizona Board Of Regents On Behalf Of The University Of Arizona, The | Sterol methyltransferase gene |
| BR9913329A (pt) * | 1998-08-25 | 2002-01-15 | Univ Nevada | Manipulação de nìveis de tocoferol em plantas transgênicas |
| AU2037700A (en) * | 1998-12-03 | 2000-06-19 | E.I. Du Pont De Nemours And Company | Plant vitamin e biosynthetic enzymes |
| WO2000032791A2 (en) * | 1998-12-03 | 2000-06-08 | E.I. Du Pont De Nemours And Company | Plan lecithin:cholesterol acyltransferases |
| EP1033405A3 (de) * | 1999-02-25 | 2001-08-01 | Ceres Incorporated | DNS-fragmente mit bestimmter Sequenz und die dadurch kodierte Polypeptide |
| DE19931834A1 (de) * | 1999-07-09 | 2001-01-11 | Sungene Gmbh & Co Kgaa | Identifizierung und Überexpression einer DNA-Sequenz codierend für eine 2-Methyl-6-phytylhydrochinon-methyltransferase in Pflanzen |
-
2000
- 2000-04-12 AT AT00922076T patent/ATE446370T1/de not_active IP Right Cessation
- 2000-04-12 BR BR0010597-0A patent/BR0010597A/pt not_active IP Right Cessation
- 2000-04-12 WO PCT/US2000/009696 patent/WO2000061771A2/en not_active Ceased
- 2000-04-12 AU AU42316/00A patent/AU4231600A/en not_active Abandoned
- 2000-04-12 EP EP00922076A patent/EP1169462B1/de not_active Expired - Lifetime
- 2000-04-12 CA CA002372120A patent/CA2372120A1/en not_active Abandoned
- 2000-04-12 HK HK02103157.1A patent/HK1041608A1/zh unknown
- 2000-04-12 DE DE60043189T patent/DE60043189D1/de not_active Expired - Lifetime
- 2000-04-12 MX MXPA01010304A patent/MXPA01010304A/es unknown
- 2000-04-12 CN CN00807669A patent/CN1360637A/zh active Pending
-
2004
- 2004-01-16 US US10/647,517 patent/US7709666B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1169462B1 (de) | 2009-10-21 |
| CN1360637A (zh) | 2002-07-24 |
| EP1169462A2 (de) | 2002-01-09 |
| US20050102716A1 (en) | 2005-05-12 |
| BR0010597A (pt) | 2002-02-13 |
| DE60043189D1 (de) | 2009-12-03 |
| HK1041608A1 (zh) | 2002-07-12 |
| WO2000061771A3 (en) | 2001-07-05 |
| CA2372120A1 (en) | 2000-10-19 |
| MXPA01010304A (es) | 2002-05-06 |
| WO2000061771A9 (en) | 2001-08-02 |
| WO2000061771A2 (en) | 2000-10-19 |
| US7709666B2 (en) | 2010-05-04 |
| AU4231600A (en) | 2000-11-14 |
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