ATE447040T1 - Verbesserte zusammensetzungen und verfahren für die cdna-synthese - Google Patents

Verbesserte zusammensetzungen und verfahren für die cdna-synthese

Info

Publication number: ATE447040T1
Authority: AT; Austria
Prior art keywords: cdna; methods; compositions; cdna synthesis; kits
Prior art date: 2002-09-03

Application number

AT03749359T

Other languages

English (en)

Inventor

Ayoub Rashtchian

David Schuster

Original Assignee

Quanta Biosciences Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2002-09-03

Filing date

2003-09-03

Publication date

2009-11-15

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=31978445&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE447040(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

2003-09-03 Application filed by Quanta Biosciences Inc filed Critical Quanta Biosciences Inc

2009-11-15 Application granted granted Critical

2009-11-15 Publication of ATE447040T1 publication Critical patent/ATE447040T1/de

Links

239000000203 mixture Substances 0.000 title abstract 4
238000000034 method Methods 0.000 title abstract 3
238000010804 cDNA synthesis Methods 0.000 title abstract 2
239000002299 complementary DNA Substances 0.000 abstract 4
102100034343 Integrase Human genes 0.000 abstract 2
108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 2
230000003321 amplification Effects 0.000 abstract 2
238000003199 nucleic acid amplification method Methods 0.000 abstract 2
239000000872 buffer Substances 0.000 abstract 1
238000006243 chemical reaction Methods 0.000 abstract 1
239000003153 chemical reaction reagent Substances 0.000 abstract 1
238000010790 dilution Methods 0.000 abstract 1
239000012895 dilution Substances 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
238000003752 polymerase chain reaction Methods 0.000 abstract 1
238000002360 preparation method Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Chemical Kinetics & Catalysis (AREA)
Analytical Chemistry (AREA)
Bioinformatics & Computational Biology (AREA)
Plant Pathology (AREA)
Crystallography & Structural Chemistry (AREA)
Immunology (AREA)
General Chemical & Material Sciences (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)

AT03749359T 2002-09-03 2003-09-03 Verbesserte zusammensetzungen und verfahren für die cdna-synthese ATE447040T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US40724802P	2002-09-03	2002-09-03
PCT/US2003/027520 WO2004021986A2 (en)	2002-09-03	2003-09-03	Improved compositions and methods for cdna synthesis

Publications (1)

Publication Number	Publication Date
ATE447040T1 true ATE447040T1 (de)	2009-11-15

Family

ID=31978445

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT03749359T ATE447040T1 (de)	2002-09-03	2003-09-03	Verbesserte zusammensetzungen und verfahren für die cdna-synthese

Country Status (10)

Country	Link
US (5)	US7638612B2 (de)
EP (3)	EP1534850B1 (de)
AT (1)	ATE447040T1 (de)
AU (1)	AU2003268397A1 (de)
CA (1)	CA2497420C (de)
DE (1)	DE60329853D1 (de)
DK (1)	DK2143803T3 (de)
ES (1)	ES2579803T3 (de)
PT (1)	PT2143803T (de)
WO (1)	WO2004021986A2 (de)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP2264045B1 (de) *	1996-08-14	2015-10-21	Life Technologies Corporation	Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren
ATE447040T1 (de)	2002-09-03	2009-11-15	Quanta Biosciences Inc	Verbesserte zusammensetzungen und verfahren für die cdna-synthese
EP1678327A4 (de) *	2003-10-16	2007-10-10	Genomic Health Inc	Qrt-pcr-testsystem zur erstellung von genexpressionsprofilen
US8835358B2 (en)	2009-12-15	2014-09-16	Cellular Research, Inc.	Digital counting of individual molecules by stochastic attachment of diverse labels
WO2012054727A1 (en)	2010-10-22	2012-04-26	Bio-Rad Laboratories, Inc.	Reverse transcriptase mixtures with improved storage stability
JP5731167B2 (ja) *	2010-10-29	2015-06-10	シスメックス株式会社	一本鎖ｃＤＮＡの合成方法、マイクロアレイ用試料の調製方法および核酸の検出方法
US10011876B2 (en)	2010-11-23	2018-07-03	Krisani Biosciences Pvt. Ltd	Method and system for prognosis and treatment of diseases using portfolio of genes
ES2904816T3 (es)	2012-02-27	2022-04-06	Becton Dickinson Co	Composiciones para recuento molecular
US20140199749A1 (en) *	2013-01-15	2014-07-17	Jun Euihum Lee	Compositions and Methods for cDNA Synthesis
JP6431522B2 (ja) *	2013-03-15	2018-11-28	アボツト・モレキユラー・インコーポレイテツド	核酸の精製のための１工程法
EP3480321B8 (de)	2013-08-28	2021-03-10	Becton, Dickinson and Company	Massiv parallele einzelzellanalyse
ES2548299B2 (es) *	2014-03-13	2016-05-13	Universidad De Málaga	Firma de microARN como indicador del riesgo de recurrencia temprana en pacientes con cáncer de mama
US10975371B2 (en) *	2014-04-29	2021-04-13	Illumina, Inc.	Nucleic acid sequence analysis from single cells
US20160097085A1 (en) *	2014-10-03	2016-04-07	Jun Euihum Lee	Compositions and Methods for cDNA Synthesis
ES2836802T3 (es)	2015-02-27	2021-06-28	Becton Dickinson Co	Códigos de barras moleculares espacialmente direccionables
EP4180535A1 (de)	2015-03-30	2023-05-17	Becton, Dickinson and Company	Verfahren und zusammensetzungen für kombinatorische barcodierung
CN107580632B (zh)	2015-04-23	2021-12-28	贝克顿迪金森公司	用于全转录组扩增的方法和组合物
CN115928221A (zh) *	2015-08-28	2023-04-07	Illumina公司	单细胞核酸序列分析
JP6940484B2 (ja)	2015-09-11	2021-09-29	セルラーリサーチ，インコーポレイテッド	ライブラリー正規化のための方法および組成物
US11299777B2 (en)	2016-04-04	2022-04-12	Nat Diagnostics, Inc.	Isothermal amplification components and processes
US9617587B1 (en)	2016-04-04	2017-04-11	Nat Diagnostics, Inc.	Isothermal amplification components and processes
US10301677B2 (en)	2016-05-25	2019-05-28	Cellular Research, Inc.	Normalization of nucleic acid libraries
US10640763B2 (en)	2016-05-31	2020-05-05	Cellular Research, Inc.	Molecular indexing of internal sequences
US10202641B2 (en)	2016-05-31	2019-02-12	Cellular Research, Inc.	Error correction in amplification of samples
CN109791157B (zh)	2016-09-26	2022-06-07	贝克顿迪金森公司	使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
CN110382708A (zh)	2017-02-01	2019-10-25	赛卢拉研究公司	使用阻断性寡核苷酸进行选择性扩增
KR102790050B1 (ko)	2017-06-05	2025-04-04	백톤 디킨슨 앤드 컴퍼니	단일 세포를 위한 샘플 인덱싱
US11773441B2 (en)	2018-05-03	2023-10-03	Becton, Dickinson And Company	High throughput multiomics sample analysis
EP3788170B1 (de)	2018-05-03	2025-01-01	Becton, Dickinson and Company	Molekulare barcodierung auf gegenüberliegenden transkriptenden
US11639517B2 (en)	2018-10-01	2023-05-02	Becton, Dickinson And Company	Determining 5′ transcript sequences
EP3877520B1 (de)	2018-11-08	2025-03-19	Becton, Dickson And Company	Ganze transkriptomanalyse von einzelzellen mittels random-priming
US11492660B2 (en)	2018-12-13	2022-11-08	Becton, Dickinson And Company	Selective extension in single cell whole transcriptome analysis
US11661631B2 (en)	2019-01-23	2023-05-30	Becton, Dickinson And Company	Oligonucleotides associated with antibodies
US12071617B2 (en)	2019-02-14	2024-08-27	Becton, Dickinson And Company	Hybrid targeted and whole transcriptome amplification
EP4707405A2 (de)	2019-07-22	2026-03-11	Becton, Dickinson and Company	Einzelzellchromatin-immunpräzipitations-sequenzierungs-assay
EP4055160B1 (de)	2019-11-08	2024-04-10	Becton Dickinson and Company	Verwendung von random-rpiming zur gewinnung von v(d)j-informationen in voller länge für immunrepertoiresequenzierung
WO2021146207A1 (en)	2020-01-13	2021-07-22	Becton, Dickinson And Company	Methods and compositions for quantitation of proteins and rna
EP4097228B1 (de)	2020-01-29	2024-08-14	Becton, Dickinson and Company	Barkodierte wells zur räumlichen abbildung einzelner zellen durch sequenzierung
WO2021173719A1 (en)	2020-02-25	2021-09-02	Becton, Dickinson And Company	Bi-specific probes to enable the use of single-cell samples as single color compensation control
CN111518669A (zh) *	2020-05-07	2020-08-11	安徽科技学院	一种核酸检测微流控芯片及其应用
WO2021231779A1 (en)	2020-05-14	2021-11-18	Becton, Dickinson And Company	Primers for immune repertoire profiling
ES2987035T3 (es)	2020-06-02	2024-11-13	Becton Dickinson Co	Oligonucleótidos y perlas para el ensayo de expresión génica principal 5
US11932901B2 (en)	2020-07-13	2024-03-19	Becton, Dickinson And Company	Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en)	2020-07-31	2022-02-03	Becton, Dickinson And Company	Single cell assay for transposase-accessible chromatin
US11739443B2 (en)	2020-11-20	2023-08-29	Becton, Dickinson And Company	Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en)	2020-12-15	2025-08-19	Becton, Dickinson And Company	Single cell secretome analysis
CN118339306A (zh) *	2021-12-02	2024-07-12	生物辐射实验室股份有限公司	Dna:rna双链体断裂的用途

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4683195A (en)	1986-01-30	1987-07-28	Cetus Corporation	Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en)	1985-03-28	1987-07-28	Cetus Corporation	Process for amplifying nucleic acid sequences
US5693517A (en) *	1987-06-17	1997-12-02	Roche Molecular Systems, Inc.	Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5310652A (en) *	1986-08-22	1994-05-10	Hoffman-La Roche Inc.	Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5561058A (en)	1986-08-22	1996-10-01	Hoffmann-La Roche Inc.	Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5244797B1 (en)	1988-01-13	1998-08-25	Life Technologies Inc	Cloned genes encoding reverse transcriptase lacking rnase h activity
US5747296A (en) *	1992-05-11	1998-05-05	Corvas International, Inc.	Method of detecting neutophil inhibitory factor mimics
US6214542B1 (en) *	1992-10-20	2001-04-10	The United States Of America As Represented By The Department Of Health And Human Services	Quantification of indicators of fibrosis
DE4336266A1 (de) *	1993-10-23	1995-04-27	Boehringer Mannheim Gmbh	Stabilisierte flüssige Mischungen für die Markierung von Nukleinsäuren
US5556771A (en) *	1995-02-10	1996-09-17	Gen-Probe Incorporated	Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
EP0912766B2 (de) *	1996-06-04	2011-12-14	University of Utah Research Foundation	Überwachung der hybridisierung während pcr
EP2264045B1 (de)	1996-08-14	2015-10-21	Life Technologies Corporation	Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren
ES2355739T3 (es) *	1997-04-03	2011-03-30	Life Technologies Corporation	Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).
WO1999002700A1 (en) *	1997-07-10	1999-01-21	Human Genome Sciences, Inc.	HISTIDINE KINASE TWO-COMPONENT IN $i(CANDIDA ALBICANS)
US6087489A (en) *	1998-06-02	2000-07-11	Isis Pharmaceuticals Inc.	Antisense oligonucleotide modulation of human thymidylate synthase expression
US6406891B1 (en) *	1998-09-28	2002-06-18	Board Of Regents, The University Of Texas System	Dual RT procedure for cDNA synthesis
US6300069B1 (en) *	1999-05-03	2001-10-09	Qiagen Gmbh	Generation and amplification of nucleic acids from ribonucleic acids
US6083727A (en) *	1999-06-30	2000-07-04	Incyte Pharmaceuticals Inc.	Methods and compositions for producing 5' enriched cDNA libraries
ATE481499T1 (de) *	1999-09-24	2010-10-15	Ambion Inc	Cocktail von nukleaseinhibitoren
US6300073B1 (en)	1999-10-01	2001-10-09	Clontech Laboratories, Inc.	One step RT-PCR methods, enzyme mixes and kits for use in practicing the same
US7078208B2 (en) *	2000-05-26	2006-07-18	Invitrogen Corporation	Thermostable reverse transcriptases and uses thereof
US6521411B2 (en)	2000-09-28	2003-02-18	Transgenomic, Inc.	Method and system for the preparation of cDNA
AU2002236524A1 (en)	2000-11-28	2002-06-11	Rosetta Inpharmatics, Inc.	In vitro transcription method for rna amplification
CN1384203A (zh) *	2001-04-30	2002-12-11	香港科技创业股份有限公司	具有高度延伸专一性的dna低温循环延伸反应方法
ATE447040T1 (de)	2002-09-03	2009-11-15	Quanta Biosciences Inc	Verbesserte zusammensetzungen und verfahren für die cdna-synthese

2003
- 2003-09-03 AT AT03749359T patent/ATE447040T1/de not_active IP Right Cessation
- 2003-09-03 ES ES09013546.8T patent/ES2579803T3/es not_active Expired - Lifetime
- 2003-09-03 PT PT90135468T patent/PT2143803T/pt unknown
- 2003-09-03 EP EP03749359A patent/EP1534850B1/de not_active Expired - Lifetime
- 2003-09-03 EP EP16164558.5A patent/EP3067429A1/de not_active Withdrawn
- 2003-09-03 DK DK09013546.8T patent/DK2143803T3/en active
- 2003-09-03 DE DE60329853T patent/DE60329853D1/de not_active Expired - Lifetime
- 2003-09-03 AU AU2003268397A patent/AU2003268397A1/en not_active Abandoned
- 2003-09-03 CA CA2497420A patent/CA2497420C/en not_active Expired - Lifetime
- 2003-09-03 WO PCT/US2003/027520 patent/WO2004021986A2/en not_active Ceased
- 2003-09-03 US US10/653,416 patent/US7638612B2/en not_active Expired - Lifetime
- 2003-09-03 EP EP09013546.8A patent/EP2143803B1/de not_active Revoked
2005
- 2005-09-29 US US11/237,922 patent/US7470515B2/en not_active Expired - Lifetime
2009
- 2009-12-08 US US12/632,802 patent/US8420324B2/en not_active Expired - Fee Related
2013
- 2013-03-04 US US13/783,780 patent/US20130244286A1/en not_active Abandoned
2015
- 2015-10-08 US US14/878,751 patent/US9988660B2/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
EP2143803B1 (de)	2016-04-13
EP3067429A1 (de)	2016-09-14
EP1534850A4 (de)	2007-01-03
EP1534850B1 (de)	2009-10-28
PT2143803T (pt)	2016-07-14
EP3067429A9 (de)	2016-10-26
US20130244286A1 (en)	2013-09-19
DK2143803T3 (en)	2016-08-01
US8420324B2 (en)	2013-04-16
EP1534850A2 (de)	2005-06-01
US20060068436A1 (en)	2006-03-30
US9988660B2 (en)	2018-06-05
US7638612B2 (en)	2009-12-29
WO2004021986A2 (en)	2004-03-18
US20160046975A1 (en)	2016-02-18
EP2143803A1 (de)	2010-01-13
US7470515B2 (en)	2008-12-30
US20110151520A1 (en)	2011-06-23
AU2003268397A1 (en)	2004-03-29
CA2497420A1 (en)	2004-03-18
US20040110201A1 (en)	2004-06-10
DE60329853D1 (de)	2009-12-10
WO2004021986A3 (en)	2004-06-24
CA2497420C (en)	2016-04-19
AU2003268397A8 (en)	2004-03-29
ES2579803T3 (es)	2016-08-16

Legal Events

Date	Code	Title	Description
2010-04-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
ATE447040T1 (de)	2009-11-15	Verbesserte zusammensetzungen und verfahren für die cdna-synthese
Nolan et al.	2013	PCR technology: current innovations
EP2272952B8 (de)	2012-08-15	Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
WO2007041342A3 (en)	2007-10-04	Labeled nucleotide analogs and uses therefor
WO2003008624A3 (en)	2004-03-04	Nucleic acid amplification using nicking agents
WO2008033442A3 (en)	2008-10-16	Methods and compositions for performing low background multiplex nucleic acid amplification reactions
WO2001094630A3 (en)	2003-08-21	Compositions and methods for array-based nucleic acid hybridization
WO2000050871A8 (en)	2001-10-11	Microstructures for use in biological assays and reactions
DE60034396D1 (de)	2007-05-31	Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure
WO2006135684A3 (en)	2007-06-21	Methods and kits for sense rna synthesis
IL131966A0 (en)	2001-03-19	An in vitro method for construction of a dna library
WO2005030984A3 (en)	2005-12-22	Method for small volume nucleic acid synthesis
WO2021228905A3 (en)	2022-01-13	Mutant polymerases and methods of using the same
WO2001025483A3 (en)	2002-01-24	Compositions for dna amplification, synthesis, and mutagenesis
WO2008016562A3 (en)	2008-07-17	Nucleic acid amplification using a reversibly modified oligonucleotide
WO2009135214A3 (en)	2010-03-11	Rna polyphosphatase compositions, kits, and uses thereof
WO2002086155A3 (en)	2003-03-06	Method for the preferential nucleic acid amplification of selected nucleic acid regions
ATE484515T1 (de)	2010-10-15	Dna-polymerase-mischungen und mutante dna- polymerasen
WO2004068111A3 (en)	2004-12-09	Methods and compositions for rna detection and quantitation
WO2003002587A3 (en)	2003-08-28	Phosphoramide containing nucleic acid-based compounds and libraries as antivirals
WO2005121373A3 (en)	2007-05-10	Hybridization of pna probes in alcohol solutions
WO2005118866A9 (en)	2009-02-26	COMPOSITIONS AND METHODS FOR SYNTHESIZING cDNA
WO2006081623A8 (en)	2006-12-07	Double-stranded oligonucleotides and uses therefor
WO2009029648A3 (en)	2009-04-30	Methods and compositions for pcr
ATE491810T1 (de)	2011-01-15	Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung