ATE448325T1 - Asynchrones pcr priming - Google Patents
Asynchrones pcr primingInfo
- Publication number
- ATE448325T1 ATE448325T1 AT01946152T AT01946152T ATE448325T1 AT E448325 T1 ATE448325 T1 AT E448325T1 AT 01946152 T AT01946152 T AT 01946152T AT 01946152 T AT01946152 T AT 01946152T AT E448325 T1 ATE448325 T1 AT E448325T1
- Authority
- AT
- Austria
- Prior art keywords
- probe
- reporter dye
- asynchronous
- stranded
- annealed
- Prior art date
Links
- 230000037452 priming Effects 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 5
- 238000002844 melting Methods 0.000 abstract 4
- 230000008018 melting Effects 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 238000005382 thermal cycling Methods 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 239000003298 DNA probe Substances 0.000 abstract 1
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 238000000137 annealing Methods 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20988300P | 2000-06-06 | 2000-06-06 | |
| US09/875,211 US6887664B2 (en) | 2000-06-06 | 2001-06-05 | Asynchronous primed PCR |
| PCT/US2001/018464 WO2001094638A2 (en) | 2000-06-06 | 2001-06-06 | Asynchronous primed pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE448325T1 true ATE448325T1 (de) | 2009-11-15 |
Family
ID=26904613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01946152T ATE448325T1 (de) | 2000-06-06 | 2001-06-06 | Asynchrones pcr priming |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6887664B2 (de) |
| EP (3) | EP2316975A1 (de) |
| JP (1) | JP2004509613A (de) |
| AT (1) | ATE448325T1 (de) |
| AU (1) | AU6823501A (de) |
| CA (1) | CA2412413A1 (de) |
| DE (1) | DE60140487D1 (de) |
| WO (1) | WO2001094638A2 (de) |
Families Citing this family (51)
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| US6887664B2 (en) * | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
| CA2439402A1 (en) * | 2001-03-02 | 2002-09-12 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Pcr method |
| US20030175749A1 (en) | 2001-12-08 | 2003-09-18 | Jong-Yoon Chun | Annealing control primer and its uses |
| US7198897B2 (en) * | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
| EP1942196B1 (de) | 2001-12-19 | 2012-05-30 | Brandeis University | Late-PCR |
| JP2003199568A (ja) * | 2002-01-10 | 2003-07-15 | Nichirei Corp | Dna増幅反応の効率向上方法 |
| CA2501144C (en) | 2002-10-23 | 2015-10-06 | University Of Utah Research Foundation | Amplicon melting analysis with saturation dyes |
| JP4280519B2 (ja) * | 2003-03-07 | 2009-06-17 | アークレイ株式会社 | Dna増幅法およびそのためのキット |
| JP2005000162A (ja) * | 2003-05-19 | 2005-01-06 | Canon Inc | 核酸のpcr増幅方法、pcrプライマー・セット、pcr増幅産物、ならびに、該増幅方法を利用する核酸の検出方法 |
| US20040235032A1 (en) * | 2003-05-19 | 2004-11-25 | Canon Kabushiki Kaisha | PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method |
| JP4322554B2 (ja) * | 2003-05-19 | 2009-09-02 | 株式会社ニチレイフーズ | Dna増幅反応の効率向上方法 |
| AU2003276765A1 (en) * | 2003-11-10 | 2005-05-26 | Seegene, Inc. | Method for amplifying unknown dna sequence adjacent to known sequence |
| US7927797B2 (en) * | 2004-01-28 | 2011-04-19 | 454 Life Sciences Corporation | Nucleic acid amplification with continuous flow emulsion |
| US7481997B1 (en) | 2004-02-12 | 2009-01-27 | Montana State University | Snow mountain virus genome sequence, virus-like particles and methods of use |
| US7387887B2 (en) * | 2004-04-20 | 2008-06-17 | University Of Utah Research Foundation | Nucleic acid melting analysis with saturation dyes |
| US9657347B2 (en) | 2004-04-20 | 2017-05-23 | University of Utah Research Foundation and BioFire Defense, LLC | Nucleic acid melting analysis with saturation dyes |
| US20060228726A1 (en) * | 2005-01-06 | 2006-10-12 | Martin Patrick K | Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification |
| JP2008535518A (ja) | 2005-04-14 | 2008-09-04 | アプレラ コーポレイション | プソイドイソシトシン核酸塩基誘導体を含む3’改変オリゴヌクレオチド、およびプライマーまたはプローブとしてのその適用 |
| EP1880021A2 (de) * | 2005-05-02 | 2008-01-23 | Stratagene California | Oligonukleotidsonde/primerzusammensetzungen und verfahren zum nachweis von oligonukleotiden |
| JP4879975B2 (ja) * | 2005-05-31 | 2012-02-22 | アプライド バイオシステムズ リミテッド ライアビリティー カンパニー | 短い核酸のマルチプレックス増幅 |
| CN101495647B (zh) * | 2005-06-02 | 2014-12-17 | 阿德万德克斯公司 | 用于微生物分析的肽核酸探针 |
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| WO2007115059A2 (en) | 2006-03-29 | 2007-10-11 | Merial Limited | Vaccine against streptococci |
| CA2651815A1 (en) | 2006-05-10 | 2007-11-22 | Dxterity Diagnostics | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
| EP2046989B1 (de) | 2006-07-17 | 2013-03-20 | Brandeis University | Spezialisierte oligonukleotide und deren verwendung bei nukleinsäureamplifikation und -nachweis |
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| FI121379B (fi) * | 2008-01-17 | 2010-10-29 | Mobidiag Oy | Menetelmä yksijuosteisen DNA:n tuottamiseksi kaksivaiheisella symmetrisellä PCR:llä sekä menetelmään tarkoitettu reagenssipakkaus |
| GB0806041D0 (en) | 2008-04-03 | 2008-05-14 | Genomica S A | Method for detection of herpesvirus in test sample |
| US8993230B2 (en) * | 2008-12-04 | 2015-03-31 | Pacific Biosciences of Californ, Inc. | Asynchronous sequencing of biological polymers |
| CA2757300C (en) | 2009-04-01 | 2018-01-09 | Dxterity Diagnostics Incorporated | Chemical ligation dependent probe amplification (clpa) |
| US9334531B2 (en) * | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| US20110039304A1 (en) * | 2009-08-12 | 2011-02-17 | President And Fellows Of Harvard College | Methods to Generate Oligonucleotide Pools and Enrich Target Nucleic Acid Sequences |
| CA2825151A1 (en) * | 2011-01-31 | 2012-08-09 | Primeradx, Inc. | Methods of nucleic acid quantification and detection using anomalous migration |
| US9745616B2 (en) | 2011-05-17 | 2017-08-29 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
| EP2722399A1 (de) | 2012-10-18 | 2014-04-23 | Roche Diagniostics GmbH | Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation |
| US10942184B2 (en) | 2012-10-23 | 2021-03-09 | Caris Science, Inc. | Aptamers and uses thereof |
| CN106879252B (zh) | 2014-06-10 | 2020-07-17 | 德克斯特里蒂诊断公司 | 用于收集和稳定生物样品的装置及方法 |
| US20170233818A1 (en) * | 2014-07-25 | 2017-08-17 | Panagene Inc. | Multiple target nucleic acid detection method using clamping probe and detection probe |
| PL3298162T3 (pl) * | 2015-05-18 | 2020-07-27 | Saga Diagnostics Ab | Wykrywanie docelowego kwasu nukleinowego i wariantów |
| US10443095B2 (en) | 2016-08-02 | 2019-10-15 | Roche Molecular Systems, Inc. | Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids |
| CN108342489A (zh) * | 2017-01-23 | 2018-07-31 | 公安部物证鉴定中心 | 一种对男性个体进行y-snp分型的方法和系统 |
| US10513730B2 (en) * | 2017-11-22 | 2019-12-24 | Safeguard Biosystems Holdings Ltd. | Asymmetric PCR methods, primers and kits |
| KR101919896B1 (ko) * | 2018-05-03 | 2018-11-19 | 주식회사 유디피아 | 단일 형광 채널 유전자증폭기기를 이용한 다중 핵산 검출 방법 |
| US20210317527A1 (en) * | 2018-08-27 | 2021-10-14 | The Regents Of The University Of California | Reporter nucleic acids for type v crispr-mediated detection |
| KR102923870B1 (ko) * | 2019-09-02 | 2026-02-06 | 주식회사 시선바이오머티리얼스 | 신규한 pna 올리고머, 이의 dna 메틸화 검출용도 및 이를 이용한 dna 메틸화 검출방법 |
| EP4087941A4 (de) * | 2020-01-10 | 2023-06-28 | Siemens Healthcare Diagnostics, Inc. | Zusammensetzungen, kits und verfahren zum durchführen von schnellen polymerase-kettenreaktionen |
| US20240102085A1 (en) * | 2022-02-15 | 2024-03-28 | Cepheid | EXPONENTIAL BASE-GREATER-THAN-BASE 2 NUCLEIC ACID AMPLIFICATION USING HIGH- AND LOW-Tm PRIMERS |
| CN118726554A (zh) * | 2024-07-03 | 2024-10-01 | 苏州海苗生物科技有限公司 | 一种条件性多重qpcr方法 |
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-
2001
- 2001-06-05 US US09/875,211 patent/US6887664B2/en not_active Expired - Lifetime
- 2001-06-06 DE DE60140487T patent/DE60140487D1/de not_active Expired - Lifetime
- 2001-06-06 EP EP10184140A patent/EP2316975A1/de not_active Withdrawn
- 2001-06-06 AT AT01946152T patent/ATE448325T1/de not_active IP Right Cessation
- 2001-06-06 AU AU6823501A patent/AU6823501A/xx active Pending
- 2001-06-06 CA CA002412413A patent/CA2412413A1/en not_active Abandoned
- 2001-06-06 WO PCT/US2001/018464 patent/WO2001094638A2/en not_active Ceased
- 2001-06-06 EP EP09014084A patent/EP2256213A1/de not_active Withdrawn
- 2001-06-06 JP JP2002502178A patent/JP2004509613A/ja active Pending
- 2001-06-06 EP EP01946152A patent/EP1368489B1/de not_active Expired - Lifetime
-
2004
- 2004-06-09 US US10/865,683 patent/US20050130178A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20050130178A1 (en) | 2005-06-16 |
| WO2001094638A2 (en) | 2001-12-13 |
| EP1368489B1 (de) | 2009-11-11 |
| CA2412413A1 (en) | 2001-12-13 |
| US6887664B2 (en) | 2005-05-03 |
| JP2004509613A (ja) | 2004-04-02 |
| DE60140487D1 (en) | 2009-12-24 |
| AU6823501A (en) | 2001-12-17 |
| EP1368489A2 (de) | 2003-12-10 |
| WO2001094638A3 (en) | 2003-07-10 |
| EP2316975A1 (de) | 2011-05-04 |
| WO2001094638A8 (en) | 2002-04-11 |
| US20030207266A1 (en) | 2003-11-06 |
| EP2256213A1 (de) | 2010-12-01 |
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