ATE450607T1 - Rhodococcus-stamm, nitrilase-gen aus rhodococcus, nitrilhydratas-gen und amidase-gen und verfahren zur herstellung carboxylsäuren unter deren verwendung - Google Patents

Rhodococcus-stamm, nitrilase-gen aus rhodococcus, nitrilhydratas-gen und amidase-gen und verfahren zur herstellung carboxylsäuren unter deren verwendung

Info

Publication number
ATE450607T1
ATE450607T1 AT00970065T AT00970065T ATE450607T1 AT E450607 T1 ATE450607 T1 AT E450607T1 AT 00970065 T AT00970065 T AT 00970065T AT 00970065 T AT00970065 T AT 00970065T AT E450607 T1 ATE450607 T1 AT E450607T1
Authority
AT
Austria
Prior art keywords
gene
carboxylic acids
rhodococcus
amidase
transformant
Prior art date
Application number
AT00970065T
Other languages
English (en)
Inventor
Hirobumi Aoki
Harumi Kamachi
Original Assignee
Showa Denko Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP30321299A external-priority patent/JP2001120290A/ja
Priority claimed from JP2000021797A external-priority patent/JP2001136977A/ja
Priority claimed from JP2000107855A external-priority patent/JP2001292772A/ja
Application filed by Showa Denko Kk filed Critical Showa Denko Kk
Application granted granted Critical
Publication of ATE450607T1 publication Critical patent/ATE450607T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00970065T 1999-10-26 2000-10-25 Rhodococcus-stamm, nitrilase-gen aus rhodococcus, nitrilhydratas-gen und amidase-gen und verfahren zur herstellung carboxylsäuren unter deren verwendung ATE450607T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP30321299A JP2001120290A (ja) 1999-10-26 1999-10-26 微生物によるカルボン酸類の製法
JP2000021797A JP2001136977A (ja) 1999-09-02 2000-01-26 ロドコッカス属細菌由来のニトリラーゼ遺伝子
JP2000107855A JP2001292772A (ja) 2000-04-10 2000-04-10 ロドコッカス属細菌由来のニトリルヒドラターゼ遺伝子およびアミダーゼ遺伝子
PCT/JP2000/007464 WO2001030994A1 (en) 1999-10-26 2000-10-25 Novel rhodococcus, rhodococcus-origin nitrilase gene, nitrilehydratase gene and amidase gene and process for producing carboxylic acids by using the same

Publications (1)

Publication Number Publication Date
ATE450607T1 true ATE450607T1 (de) 2009-12-15

Family

ID=27338591

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00970065T ATE450607T1 (de) 1999-10-26 2000-10-25 Rhodococcus-stamm, nitrilase-gen aus rhodococcus, nitrilhydratas-gen und amidase-gen und verfahren zur herstellung carboxylsäuren unter deren verwendung

Country Status (11)

Country Link
US (1) US7118898B1 (de)
EP (1) EP1142997B1 (de)
KR (1) KR100540991B1 (de)
CN (1) CN1340101A (de)
AT (1) ATE450607T1 (de)
AU (1) AU7957100A (de)
CA (1) CA2356741C (de)
DE (1) DE60043424D1 (de)
EA (1) EA200100718A1 (de)
NO (1) NO328690B1 (de)
WO (1) WO2001030994A1 (de)

Families Citing this family (22)

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Publication number Priority date Publication date Assignee Title
EP1599584B1 (de) 2003-02-27 2009-07-01 Basf Se Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäuren
DE10315376B4 (de) * 2003-04-03 2005-07-14 Stockhausen Gmbh Verfahren zur Kultivierung des Nitrilhydratase-produzierenden Stammes RHODOCOCCUS RHODOCHROUS M33
DE602004029180D1 (de) * 2003-10-31 2010-10-28 Daiichi Fine Chem Co Ltd Neue plasmide und deren nutzung
AU2005248259B2 (en) * 2004-05-26 2010-07-08 Mitsubishi Chemical Corporation Improved nitrile hydratase
US7148051B2 (en) * 2004-08-16 2006-12-12 E. I. Du Pont De Nemours And Company Production of 3-hydroxycarboxylic acid using nitrilase
EP1929026A4 (de) * 2005-09-22 2011-12-28 Astrazeneca Ab Neues verfahren zur umwandlung aromatischer halo-substituierter dinitrile in halo-substituierte cyan-carboxylsäuren
US20100267100A1 (en) * 2007-03-21 2010-10-21 Ling Hua Enzymatic synthesis of optically pure beta-hydroxy carboxylic acids and their derivatives
US20090004720A1 (en) * 2007-06-26 2009-01-01 Ling Hua Smart Biocatalysts For Organic Synthesis
WO2009117843A1 (zh) 2008-03-24 2009-10-01 清华大学 敲除酰胺酶基因的产腈水合酶工程菌及其构建方法和应用
CN101434926B (zh) * 2008-10-31 2010-08-11 云南大学 一株可代谢尼古丁的红球菌及其应用
CN101875907B (zh) * 2009-04-28 2013-07-10 上海市农药研究所 一种可水解3-羟基丁腈的微生物菌株
KR101223663B1 (ko) 2011-01-26 2013-01-21 재단법인 경북해양바이오산업연구원 나이트릴레이즈 ｖｍｎ１을 이용한 카르복실산의 제조방법
CN103757068B (zh) * 2014-01-10 2016-06-29 江苏清泉化学股份有限公司 一种苯甲酸衍生物的制备方法
CN105420154B (zh) * 2015-12-16 2020-03-17 清华大学 双基因敲除重组红球菌、构建方法及其应用
EP3564368B1 (de) * 2016-12-28 2025-03-26 Mitsui Chemicals, Inc. Mutierte nitrilhydratase, nukleinsäure zur codierung der besagten mutierten nitrilhydratase, expressionsvektor und transformant mit der besagten nukleinsäure, herstellungsverfahren für besagte mutierte nitrilhydratase und herstellungsverfahren für amidverbindung
CN109136301A (zh) * 2018-09-19 2019-01-04 安徽瑞邦生物科技有限公司 一种水解制备烟酸的方法
CN109295124A (zh) * 2018-10-24 2019-02-01 张刘兵 一种水解制备烟酸的方法
CN110205315B (zh) * 2019-05-30 2021-01-01 中国石油大学(华东) 腈水解酶XiNit2及其编码基因和应用
CN110499274B (zh) * 2019-09-18 2021-11-23 清华大学 一种基因工程红球菌及其构建方法与应用
CN111378640A (zh) * 2020-03-12 2020-07-07 东莞市东阳光生物合成药有限公司 制备右旋酰胺酮洛芬的方法
WO2024197480A1 (zh) * 2023-03-24 2024-10-03 浙江九洲药业股份有限公司 一种利用酰胺酶合成右旋酮洛芬的方法
CN119162158B (zh) * 2024-11-21 2025-03-14 百葵锐(天津)生物科技有限公司 一种在红球菌中高效表达酰胺酶的方法及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629700A (en) 1983-11-30 1986-12-16 Standard Oil Company (Indiana) Selective conversion of cyano compounds to amides and carboxylic acids
CN85107055A (zh) 1984-10-01 1986-06-10 诺沃工业联合股票公司 酶促工艺方法
AU627648B2 (en) 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
EP0502476B1 (de) 1991-03-04 2001-07-18 Mitsubishi Rayon Co., Ltd. Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren
JPH0799980A (ja) * 1993-10-05 1995-04-18 Japan Energy Corp ニトリラーゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、およびこれを含有する形質転換体によるニトリル類からカルボン酸の製造法
RU2053300C1 (ru) 1993-12-17 1996-01-27 Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Штамм бактерий rhodococcus rhodochrous - продуцент нитрилгидратазы
AU707650B2 (en) * 1995-08-09 1999-07-15 Ciba Specialty Chemicals Water Treatments Limited Processes for the production of amidase
EP0853663A1 (de) * 1995-10-06 1998-07-22 E.I. Du Pont De Nemours And Company Nukleinsäurefragmente, die für stereospezifische nitrilhydratase und amidase kodieren, sowie rekombinante mikroorganismen, die diese enzyme exprimieren und die für die produktion von chiralen amiden und säuren verwendet werden können
DE19743683A1 (de) * 1997-10-02 1999-04-08 Basf Ag Verfahren zur Änderung der Substratspezifität von Enzymen
JP2001136977A (ja) * 1999-09-02 2001-05-22 Showa Denko Kk ロドコッカス属細菌由来のニトリラーゼ遺伝子
JP2001069978A (ja) * 1999-09-02 2001-03-21 Showa Denko Kk ロドコッカス属細菌由来のニトリルヒドラターゼ遺伝子およびアミダーゼ遺伝子
JP2001292772A (ja) 2000-04-10 2001-10-23 Showa Denko Kk ロドコッカス属細菌由来のニトリルヒドラターゼ遺伝子およびアミダーゼ遺伝子
DE10010149A1 (de) * 2000-03-03 2001-09-06 Basf Ag Nitrilase aus Rhodococcus rhodochrous NCIMB 11216

Also Published As

Publication number Publication date
KR20010084883A (ko) 2001-09-06
NO20013209D0 (no) 2001-06-26
AU7957100A (en) 2001-05-08
KR100540991B1 (ko) 2006-01-10
EP1142997B1 (de) 2009-12-02
CA2356741C (en) 2011-01-25
NO20013209L (no) 2001-07-03
CA2356741A1 (en) 2001-05-03
EA200100718A1 (ru) 2001-12-24
DE60043424D1 (de) 2010-01-14
US7118898B1 (en) 2006-10-10
EP1142997A1 (de) 2001-10-10
CN1340101A (zh) 2002-03-13
NO328690B1 (no) 2010-04-26
WO2001030994A1 (en) 2001-05-03
EP1142997A4 (de) 2004-03-24

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