ATE452187T1 - Methode zur isolation von nukleinsäuren - Google Patents

Methode zur isolation von nukleinsäuren

Info

Publication number
ATE452187T1
ATE452187T1 AT04758687T AT04758687T ATE452187T1 AT E452187 T1 ATE452187 T1 AT E452187T1 AT 04758687 T AT04758687 T AT 04758687T AT 04758687 T AT04758687 T AT 04758687T AT E452187 T1 ATE452187 T1 AT E452187T1
Authority
AT
Austria
Prior art keywords
nucleic acid
molecular weight
isolation
cell
nucleic acids
Prior art date
Application number
AT04758687T
Other languages
English (en)
Inventor
Kevin Mckernan
Junaid Ziauddin
Original Assignee
Agencourt Bioscience Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agencourt Bioscience Corp filed Critical Agencourt Bioscience Corp
Application granted granted Critical
Publication of ATE452187T1 publication Critical patent/ATE452187T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
AT04758687T 2003-04-02 2004-04-01 Methode zur isolation von nukleinsäuren ATE452187T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/406,141 US20040197780A1 (en) 2003-04-02 2003-04-02 Method for isolating nucleic acids
PCT/US2004/009960 WO2004090132A2 (en) 2003-04-02 2004-04-01 Method for isolating nucleic acids

Publications (1)

Publication Number Publication Date
ATE452187T1 true ATE452187T1 (de) 2010-01-15

Family

ID=33097261

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04758687T ATE452187T1 (de) 2003-04-02 2004-04-01 Methode zur isolation von nukleinsäuren

Country Status (7)

Country Link
US (2) US20040197780A1 (de)
EP (1) EP1608752B1 (de)
AT (1) ATE452187T1 (de)
DE (1) DE602004024663D1 (de)
ES (1) ES2341959T3 (de)
PL (1) PL1608752T3 (de)
WO (1) WO2004090132A2 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999058664A1 (en) * 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
EP1349951A4 (de) * 2001-01-09 2005-08-24 Whitehead Biomedical Inst Verfahren und reagenzien zur isolierung von nukleinsäuren
US20040197780A1 (en) * 2003-04-02 2004-10-07 Agencourt Bioscience Corporation Method for isolating nucleic acids
US20060078923A1 (en) * 2003-04-02 2006-04-13 Mckernan Kevin Method for isolating nucleic acids
EP1778877B1 (de) 2004-07-28 2009-10-21 Canon U.S. Life Sciences, Inc. Verfahren zur überwachung genomischer dna von organismen
JP2008510456A (ja) * 2004-07-30 2008-04-10 アジェンコート バイオサイエンス コーポレーション 多官能基コート化固相担体を用いる核酸の単離方法
US8569477B2 (en) * 2005-02-11 2013-10-29 Life Technologies As Method for isolating nucleic acids comprising the use of ethylene glycol multimers
KR101544351B1 (ko) 2005-02-18 2015-08-13 캐논 유.에스. 라이프 사이언시즈, 인크. 유기체의 게놈 ｄｎａ를 확인하기 위한 장치 및 방법
CN101300352B (zh) 2005-09-01 2013-03-20 佳能美国生命科学公司 检测、分析和鉴定基因组dna的方法和分子诊断装置
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
US20090036325A1 (en) * 2007-05-25 2009-02-05 Applera Corporation Directed assembly of amplicons to enhance read pairing signature with massively parallel short read sequencers
MY157651A (en) * 2007-07-23 2016-07-15 Yiap Beow Chin A method, kit and apparatus for extracting biological materials
WO2009020609A2 (en) * 2007-08-06 2009-02-12 Nanogen, Inc. Isolation of nucleic acids molecules using modified solid supports
WO2009049147A2 (en) * 2007-10-10 2009-04-16 Philadelphia Health & Education Corporation D/B/A Drexel University College Of Medicine Method of tumor screening by detection of tumour marker in low molecular weight nucleic acid from urine
TWI407994B (zh) 2009-10-22 2013-09-11 Ind Tech Res Inst 分離核酸的方法、試劑及套組
DE102012012523B4 (de) 2012-06-26 2015-02-12 Magnamedics Gmbh Reinigung von Nukleinsäuren
WO2017158425A1 (en) 2016-03-18 2017-09-21 Andrew Alliance S.A. Methods and apparatus for bead manipulation in a tip of a liquid handler
US20200080074A1 (en) 2016-12-15 2020-03-12 Qiagen Gmbh Method for isolating highly pure nucleic acid with magnetic particles
EP4127731A1 (de) 2020-04-03 2023-02-08 Andrew Alliance S.A Perlenmanipulation in einem verbrauchsmaterial

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4695393A (en) * 1983-05-12 1987-09-22 Advanced Magnetics Inc. Magnetic particles for use in separations
US4628037A (en) * 1983-05-12 1986-12-09 Advanced Magnetics, Inc. Binding assays employing magnetic particles
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4698302A (en) * 1983-05-12 1987-10-06 Advanced Magnetics, Inc. Enzymatic reactions using magnetic particles
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5523231A (en) * 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
JP3696238B2 (ja) * 1993-08-30 2005-09-14 プロメガ・コーポレイシヨン 核酸精製用組成物及び方法
US5443989A (en) * 1993-10-25 1995-08-22 Beth Israel Hospital Association Method for assessing fetal lung maturity using amniotic fluid samples
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
GB9411572D0 (en) * 1994-06-09 1994-08-03 Amersham Int Plc Magnetic bead precipitation method
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5939291A (en) * 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
KR20010032806A (ko) * 1997-12-06 2001-04-25 매튜 존 베이커 핵산 분리방법
US5972613A (en) * 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
WO1999058664A1 (en) * 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
GB9915398D0 (en) * 1999-07-02 1999-09-01 Baker Matthew J Magnetic particles
EP1349951A4 (de) * 2001-01-09 2005-08-24 Whitehead Biomedical Inst Verfahren und reagenzien zur isolierung von nukleinsäuren
US6534363B2 (en) * 2001-03-12 2003-03-18 Advanced Micro Devices, Inc. High voltage oxidation method for highly reliable flash memory devices
US20040197780A1 (en) * 2003-04-02 2004-10-07 Agencourt Bioscience Corporation Method for isolating nucleic acids
US20060078923A1 (en) * 2003-04-02 2006-04-13 Mckernan Kevin Method for isolating nucleic acids
JP2008510456A (ja) * 2004-07-30 2008-04-10 アジェンコート バイオサイエンス コーポレーション 多官能基コート化固相担体を用いる核酸の単離方法

Also Published As

Publication number Publication date
US20070054285A1 (en) 2007-03-08
ES2341959T3 (es) 2010-06-30
WO2004090132A3 (en) 2004-12-02
WO2004090132A2 (en) 2004-10-21
US20040197780A1 (en) 2004-10-07
EP1608752B1 (de) 2009-12-16
DE602004024663D1 (de) 2010-01-28
EP1608752A2 (de) 2005-12-28
PL1608752T3 (pl) 2010-09-30

Similar Documents

Publication Publication Date Title
ATE452187T1 (de) Methode zur isolation von nukleinsäuren
WO2019103442A3 (ko) CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도
EP4219747A3 (de) Verfahren zur herstellung sterilisierter zusammensetzungen zur stabilisierung extrazellulärer nukleinsäuren
WO2011101811A3 (en) Improved meganuclease recombination system
DE602007012858D1 (de) Verfahren zur durchführung homologer rekombinationen in pflanzen
DK1513952T3 (da) Sammensætninger og fremgangsmåder til frembringelse af nukleinsyre af sputum
WO2010083250A3 (en) Single-cell nucleic acid analysis
US20190194648A1 (en) Construction method for serial sequencing libraries of rad tags
EP4397759A3 (de) Zusammensetzung zur spaltung einer ziel-dna mit einer für die ziel-dna spezifischen leit-rna und für cas-protein codierende nukleinsäure oder cas-protein sowie verwendung davon
RU2014127702A (ru) Модифицированные Cascade-рибонуклеопротеины и их применения
WO2007014275A3 (en) Targeted integration and expression of exogenous nucleic acid sequences
EP3495478A3 (de) Erkennungssequenzen für meganukleasen aus i-crei und ihre verwendung
EP4028515A1 (de) Neue typ-ii- und typ-v crispr-cas-rna-gesteuerte endonukleasen der klasse 2
WO2002044400A3 (en) Compositions and methods for extracting a nucleic acid
EP3935185A1 (de) Zusammensetzungen und verfahren zur markierung von nukleinsäuren und sequenzierung und analyse davon
MXPA06000092A (es) Transformacion de cloroplasto de la lenteja de agua.
JP2020518247A5 (de)
WO2005081632A3 (en) Enzymes, cells and methods for site specific recombination at asymmetric sites
WO2004010925A3 (en) Mammalian genes involved in viral infection and tumor suppression
EP1642970A4 (de) Verfahren zur herstellung eines transgenen organismus unter verwendung von methylierung und system dafür
WO2008066858A3 (en) Method of separating target dna from mixed dna
WO2004013333A3 (de) Revertierung der negativ-selektiven wirkung von negativen markerproteinen als selektionsverfahren
CN103757053A (zh) 一种特异性的dna病毒基因组定点改造及筛选方法
US11761033B2 (en) Methods to amplify highly uniform and less error prone nucleic acid libraries
NO20063278L (no) Hurtigfremstilling av nukleinsyrer ved enzymatisk fordoyelse

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties