ATE454456T1 - Intein enthaltende multigen-expressionskonstrukte - Google Patents
Intein enthaltende multigen-expressionskonstrukteInfo
- Publication number
- ATE454456T1 ATE454456T1 AT01909045T AT01909045T ATE454456T1 AT E454456 T1 ATE454456 T1 AT E454456T1 AT 01909045 T AT01909045 T AT 01909045T AT 01909045 T AT01909045 T AT 01909045T AT E454456 T1 ATE454456 T1 AT E454456T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- plants
- constructs
- fused
- splicing unit
- Prior art date
Links
- 230000017730 intein-mediated protein splicing Effects 0.000 title abstract 4
- 229940098006 multigen Drugs 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 102000004169 proteins and genes Human genes 0.000 abstract 5
- 108091026890 Coding region Proteins 0.000 abstract 2
- 108010076039 Polyproteins Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 239000005562 Glyphosate Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 abstract 1
- 229940097068 glyphosate Drugs 0.000 abstract 1
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 abstract 1
- 230000008488 polyadenylation Effects 0.000 abstract 1
- 229920000903 polyhydroxyalkanoate Polymers 0.000 abstract 1
- 230000016434 protein splicing Effects 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Revetment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18173900P | 2000-02-11 | 2000-02-11 | |
| PCT/US2001/004254 WO2001059091A2 (en) | 2000-02-11 | 2001-02-09 | Multi-gene expression constructs containing modified inteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE454456T1 true ATE454456T1 (de) | 2010-01-15 |
Family
ID=22665581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01909045T ATE454456T1 (de) | 2000-02-11 | 2001-02-09 | Intein enthaltende multigen-expressionskonstrukte |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7026526B2 (de) |
| EP (3) | EP1255846B1 (de) |
| AT (1) | ATE454456T1 (de) |
| AU (1) | AU2001236839A1 (de) |
| DE (1) | DE60140996D1 (de) |
| ES (1) | ES2336641T3 (de) |
| WO (1) | WO2001059091A2 (de) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7235716B2 (en) | 1997-06-03 | 2007-06-26 | Chromatin, Inc. | Plant centromere compositions |
| US7119250B2 (en) | 1997-06-03 | 2006-10-10 | The University Of Chicago | Plant centromere compositions |
| US7227057B2 (en) | 1997-06-03 | 2007-06-05 | Chromatin, Inc. | Plant centromere compositions |
| US7193128B2 (en) | 1997-06-03 | 2007-03-20 | Chromatin, Inc. | Methods for generating or increasing revenues from crops |
| ATE454456T1 (de) * | 2000-02-11 | 2010-01-15 | Metabolix Inc | Intein enthaltende multigen-expressionskonstrukte |
| AU2001261408A1 (en) * | 2000-05-12 | 2001-11-26 | Odyssey Pharmaceuticals, Inc. | Mapping molecular interactions in plants with protein fragments complementation assays |
| WO2003056904A2 (en) * | 2002-01-08 | 2003-07-17 | Raab Michael R | Transgenic plants expressing civps or intein modified proteins and related method |
| DK1499734T3 (da) * | 2002-04-29 | 2009-10-05 | Icon Genetics Gmbh | Fremgangsmåde til fremstilling af miljömæssigt sikre transgene organismer |
| AU2003226693B2 (en) * | 2002-05-31 | 2008-02-21 | Bayer Cropscience N.V. | Transgenic plants with controlled distribution of a trait to progeny |
| PT1818405E (pt) | 2004-04-09 | 2015-10-20 | Monsanto Technology Llc | Composições e métodos para o controlo de infestações de plantas por insectos |
| JP2008532553A (ja) | 2005-03-16 | 2008-08-21 | メタボリックス, インコーポレイテッド | 生合成経路の化学的に誘導可能な発現 |
| BRPI0613784A2 (pt) * | 2005-07-21 | 2011-02-01 | Abbott Lab | expressão de gene múltipla incluindo constructos sorf e métodos com poliproteìnas, pro-proteìnas e proteólise |
| US9150877B2 (en) * | 2007-09-27 | 2015-10-06 | Dow Agrosciences Llc | Construct and method for expressing transgenes using a Brassica bidirectional constitutive promoter |
| EP2456894A4 (de) | 2009-07-23 | 2013-09-25 | Chromatin Inc | Sorghum-zentromer-sequenzen und -minichromosomen |
| EP2477477A1 (de) | 2009-09-15 | 2012-07-25 | Metabolix, Inc. | Erzeugung von ölsamen zur hohen polyhydroxybuttersäureproduktion |
| EP2493923A1 (de) * | 2009-10-30 | 2012-09-05 | Abbott Laboratories | Sorf-konstrukte und multiple genexpression |
| US10407742B2 (en) | 2009-11-06 | 2019-09-10 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
| US8420387B2 (en) | 2009-11-06 | 2013-04-16 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
| US9464333B2 (en) | 2009-11-06 | 2016-10-11 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
| US8609967B2 (en) * | 2010-01-14 | 2013-12-17 | Kmc Music, Inc. | Top-tuning system for hand percussion instrument |
| US10443068B2 (en) | 2010-06-25 | 2019-10-15 | Agrivida, Inc. | Plants with engineered endogenous genes |
| US9598700B2 (en) | 2010-06-25 | 2017-03-21 | Agrivida, Inc. | Methods and compositions for processing biomass with elevated levels of starch |
| AU2012225487B2 (en) | 2011-03-07 | 2017-04-06 | Agrivida, Inc. | Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes |
| USD831312S1 (en) | 2014-11-14 | 2018-10-23 | Gavrieli Brands LLC | Dual-sole shoe with blue outpatch sole and contrasting upper |
| WO2013078433A1 (en) * | 2011-11-23 | 2013-05-30 | University Of Hawaii | Auto-processing domains for polypeptide expression |
| AU2013271740B2 (en) | 2012-06-07 | 2017-04-13 | Corteva Agriscience Llc | Construct and method for expressing transgenes using a Brassica bidirectional constitutive promoter |
| BR112015007466B1 (pt) | 2012-10-03 | 2022-10-11 | Agrivida, Inc | Protease inteína-modificada, cassete de expressão, hospedeiro, método de produção de uma protease e detergente |
| RU2601126C2 (ru) * | 2014-09-17 | 2016-10-27 | Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России | Способ получения биологически активных пептидов |
| EP3660053A1 (de) | 2018-11-27 | 2020-06-03 | Fraunhofer Gesellschaft zur Förderung der Angewand | Multiproteinexpressionssysteme zur schädlingsbekämpfung |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5034322A (en) | 1983-01-17 | 1991-07-23 | Monsanto Company | Chimeric genes suitable for expression in plant cells |
| US5352605A (en) | 1983-01-17 | 1994-10-04 | Monsanto Company | Chimeric genes for transforming plant cells using viral promoters |
| US5231019A (en) | 1984-05-11 | 1993-07-27 | Ciba-Geigy Corporation | Transformation of hereditary material of plants |
| US5668298A (en) | 1984-12-24 | 1997-09-16 | Eli Lilly And Company | Selectable marker for development of vectors and transformation systems in plants |
| US5420034A (en) | 1986-07-31 | 1995-05-30 | Calgene, Inc. | Seed-specific transcriptional regulation |
| US5188958A (en) | 1986-05-29 | 1993-02-23 | Calgene, Inc. | Transformation and foreign gene expression in brassica species |
| US5024944A (en) | 1986-08-04 | 1991-06-18 | Lubrizol Genetics, Inc. | Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species |
| US5276268A (en) | 1986-08-23 | 1994-01-04 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
| US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
| US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
| US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
| US5030572A (en) | 1987-04-01 | 1991-07-09 | Lubrizol Genetics, Inc. | Sunflower regeneration from cotyledons |
| US5480794A (en) * | 1987-06-29 | 1996-01-02 | Massachusetts Institute Of Technology And Metabolix, Inc. | Overproduction and purification of soluble PHA synthase |
| ATE105585T1 (de) | 1987-12-21 | 1994-05-15 | Univ Toledo | Transformation von keimenden pflanzensamen mit hilfe von agrobacterium. |
| US5614395A (en) | 1988-03-08 | 1997-03-25 | Ciba-Geigy Corporation | Chemically regulatable and anti-pathogenic DNA sequences and uses thereof |
| US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| HU218717B (hu) | 1989-03-17 | 2000-11-28 | E. I. Du Pont De Nemours And Co. | Nukleinsav-termelést fokozó növényi eredetű génfragmentek és eljárás előállításukra |
| US5629183A (en) | 1989-05-08 | 1997-05-13 | The United States Of America As Represented By The Secretary Of Agriculture | Plant transformation by gene transfer into pollen |
| US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| ATE227340T1 (de) | 1989-07-10 | 2002-11-15 | Massachusetts Inst Technology | Eine zur herstellung von polyhydroxybutyrat oder einem anderen polyhydroxyalkanoat geeignete pflanze oder pflanzliche zelle |
| US5322783A (en) | 1989-10-17 | 1994-06-21 | Pioneer Hi-Bred International, Inc. | Soybean transformation by microparticle bombardment |
| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| US5519164A (en) | 1990-02-01 | 1996-05-21 | Hoechst Aktiengesellschaft | Expression of a multigene RNA having self-splicing activity |
| NZ239977A (en) | 1990-11-14 | 1993-08-26 | Pioneer Hi Bred Int | Transforming plants by the use of agrobacterium |
| US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
| US5650554A (en) | 1991-02-22 | 1997-07-22 | Sembiosys Genetics Inc. | Oil-body proteins as carriers of high-value peptides in plants |
| WO1993020216A1 (en) | 1991-02-22 | 1993-10-14 | University Technologies International, Inc. | Oil-body protein cis-elements as regulatory signals |
| GB9304200D0 (en) | 1993-03-02 | 1993-04-21 | Sandoz Ltd | Improvements in or relating to organic compounds |
| DK152291D0 (da) | 1991-08-28 | 1991-08-28 | Danisco | Fremgangsmaade og kemiske forbindelser |
| DE69334225D1 (de) | 1992-07-07 | 2008-07-31 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
| US5527695A (en) | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
| WO1995021249A1 (en) * | 1994-02-03 | 1995-08-10 | The Scripps Research Institute | A cassette to accumulate multiple proteins through synthesis of a self-processing polypeptide |
| FR2717187B1 (fr) | 1994-03-10 | 1996-05-31 | Transgene Sa | Usage combiné de deux cassettes d'expression pour la production d'une protéine d'intérêt. |
| DE4433134A1 (de) | 1994-09-16 | 1996-03-21 | Buck Chem Tech Werke | Verfahren zur Herstellung von Polyhydroxyfettsäuren sowie rekombinanter Bakterienstämme zur Durchführung des Verfahrens |
| US5958745A (en) | 1996-03-13 | 1999-09-28 | Monsanto Company | Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants |
| US5750848A (en) | 1996-08-13 | 1998-05-12 | Monsanto Company | DNA sequence useful for the production of polyhydroxyalkanoates |
| US6117658A (en) | 1997-02-13 | 2000-09-12 | James Madison University | Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units |
| JP2001516574A (ja) | 1997-09-19 | 2001-10-02 | メタボリックス,インコーポレイテッド | 4−ヒドロキシ酸を含むポリヒドロキシアルカノエートポリマー製造のための生物学的システム |
| US6858775B1 (en) * | 1999-05-24 | 2005-02-22 | New England Biolabs, Inc. | Method for generating split, non-transferable genes that are able to express an active protein product |
| ATE454456T1 (de) * | 2000-02-11 | 2010-01-15 | Metabolix Inc | Intein enthaltende multigen-expressionskonstrukte |
-
2001
- 2001-02-09 AT AT01909045T patent/ATE454456T1/de not_active IP Right Cessation
- 2001-02-09 EP EP01909045A patent/EP1255846B1/de not_active Expired - Lifetime
- 2001-02-09 DE DE60140996T patent/DE60140996D1/de not_active Expired - Lifetime
- 2001-02-09 AU AU2001236839A patent/AU2001236839A1/en not_active Abandoned
- 2001-02-09 ES ES01909045T patent/ES2336641T3/es not_active Expired - Lifetime
- 2001-02-09 EP EP09075515.8A patent/EP2159287A3/de not_active Withdrawn
- 2001-02-09 EP EP09075516.6A patent/EP2159288A3/de not_active Withdrawn
- 2001-02-09 WO PCT/US2001/004254 patent/WO2001059091A2/en not_active Ceased
- 2001-02-09 US US09/779,957 patent/US7026526B2/en not_active Expired - Fee Related
-
2005
- 2005-06-08 US US11/147,546 patent/US7741530B2/en not_active Expired - Fee Related
-
2010
- 2010-04-21 US US12/764,516 patent/US8293971B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20100251418A1 (en) | 2010-09-30 |
| WO2001059091A3 (en) | 2001-12-20 |
| US7026526B2 (en) | 2006-04-11 |
| EP1255846B1 (de) | 2010-01-06 |
| ES2336641T3 (es) | 2010-04-15 |
| AU2001236839A1 (en) | 2001-08-20 |
| EP2159287A3 (de) | 2014-02-12 |
| WO2001059091A2 (en) | 2001-08-16 |
| EP2159288A2 (de) | 2010-03-03 |
| EP2159287A2 (de) | 2010-03-03 |
| US8293971B2 (en) | 2012-10-23 |
| DE60140996D1 (de) | 2010-02-25 |
| US7741530B2 (en) | 2010-06-22 |
| US20050283850A1 (en) | 2005-12-22 |
| EP2159288A3 (de) | 2014-02-12 |
| EP1255846A2 (de) | 2002-11-13 |
| US20020129400A1 (en) | 2002-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE454456T1 (de) | Intein enthaltende multigen-expressionskonstrukte | |
| Kumar et al. | Transgene integration in aspen: structures of integration sites and mechanism of T‐DNA integration | |
| AU2913601A (en) | Potassium channel stress-related proteins and methods of use in plants | |
| Weber et al. | A modular cloning system for standardized assembly of multigene constructs | |
| IL71906A (en) | Method for producing a baculovirus vector capable of producing a selected non-fused recombinant protein or capable of being utilized as a transfer vector;a recombinant baculovirus expression vector capable of expressing under the control of a baculovirus polyhedrin promoter a selected non-fused protein in a host insect cell;a recombinant transfer vector utilizing a baculovirus polyhedrin promoter and flanking sequences for introducing a selected gene into a baculovirus genome,or for insertion of a selected gene for a genetic manipulation of a baculovirus;and a method for the synthesis of a selected non-fused protein in a host insect cell using the expression vector | |
| WO2001077161A3 (en) | Gtp binding stress-related proteins and methods of use in plants | |
| DE60042969D1 (de) | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren | |
| AU5308698A (en) | Design principle for constructing expression constructs for gene therapy | |
| BRPI9814125A8 (pt) | Genes otimizados em plantas codificando toxinas pesticidas | |
| PL352332A1 (en) | Expression and export of interferon-alpha proteins as fc fusion proteins | |
| Vazquez‐Vilar et al. | Multigene engineering by GoldenBraid cloning: from plants to filamentous fungi and beyond | |
| ATE542913T1 (de) | Promotorvarianten zur expression von genen in einer pilzzelle | |
| WO2001034817A3 (en) | Genes encoding enzymes for lignin biosynthesis and uses thereof | |
| CN102850444A (zh) | 一对转录激活子样效应因子核酸酶l3和r1及其编码基因与应用 | |
| KR880014107A (ko) | 안지오게닌의 유전공학적 제조방법 | |
| WO2002012519A3 (en) | Counter selection strategy for gram-negative bacteria | |
| CN102702335A (zh) | 重组转录激活子样效应因子、转录激活子样效应因子核酸酶及其编码基因及应用 | |
| EP2189524A3 (de) | Proteinkinase-stressbedingte Polypeptide und Verwendungsverfahren für Pflanzen | |
| WO2005060553A3 (en) | Geraniol synthase, methods of production and uses thereof | |
| KR920004577A (ko) | 단백질 합성의 cks 방법 | |
| DE602004029158D1 (en) | Archäen-expressionssystem | |
| DE60109819D1 (de) | Expression von multiplen proteinen in transgenen pflanzen | |
| DE50111213D1 (de) | Nucleinsäuren, mit deren hilfe pflanzen mit verändertem metabolit-gehalt erzeugt werden können | |
| WO2003040344A3 (en) | Transcription factor stress-related polypeptides and methods of use in plants | |
| WATSON | Bioengineered bubbly. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |