ATE455180T1 - Biochip electroporationsgerät mit mehreren positionen zur einzelzellelectroporation - Google Patents

Biochip electroporationsgerät mit mehreren positionen zur einzelzellelectroporation

Info

Publication number
ATE455180T1
ATE455180T1 AT04706137T AT04706137T ATE455180T1 AT E455180 T1 ATE455180 T1 AT E455180T1 AT 04706137 T AT04706137 T AT 04706137T AT 04706137 T AT04706137 T AT 04706137T AT E455180 T1 ATE455180 T1 AT E455180T1
Authority
AT
Austria
Prior art keywords
electroporation
biochip
single cell
multiple positions
adhering
Prior art date
Application number
AT04706137T
Other languages
English (en)
Inventor
Stefano Vassanelli
Giorgio Cellere
Mauro Borgo
Leonardo Bandiera
Original Assignee
Narvalus S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Narvalus S R L filed Critical Narvalus S R L
Application granted granted Critical
Publication of ATE455180T1 publication Critical patent/ATE455180T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT04706137T 2004-01-29 2004-01-29 Biochip electroporationsgerät mit mehreren positionen zur einzelzellelectroporation ATE455180T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2004/000789 WO2005075656A1 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation

Publications (1)

Publication Number Publication Date
ATE455180T1 true ATE455180T1 (de) 2010-01-15

Family

ID=34833863

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04706137T ATE455180T1 (de) 2004-01-29 2004-01-29 Biochip electroporationsgerät mit mehreren positionen zur einzelzellelectroporation

Country Status (11)

Country Link
US (1) US8017367B2 (de)
EP (1) EP1720991B1 (de)
JP (1) JP4588719B2 (de)
CN (1) CN100497641C (de)
AT (1) ATE455180T1 (de)
AU (1) AU2004315392B2 (de)
CA (1) CA2554027C (de)
DE (1) DE602004025146D1 (de)
DK (1) DK1720991T3 (de)
IL (1) IL177110A0 (de)
WO (1) WO2005075656A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7266457B1 (en) * 1999-05-21 2007-09-04 Hesperos, Llc High throughput functional genomics
KR20070077320A (ko) * 2006-01-23 2007-07-26 삼성전자주식회사 세포 배양을 실시간으로 모니터링 가능한 세포 배양 칩 및그를 이용한 세포 배양 모니터링 방법
ITTO20060273A1 (it) 2006-04-12 2007-10-13 Silicon Biosystem S P A Metodi ed apparati per la selezione e/o il processamento di particellle, in particolare per la lisi selettiva e/o ottimizzata di cellule
ITTO20060278A1 (it) * 2006-04-13 2007-10-14 Silicon Biosystem S P A Metodo per la selezione e/o il processamento di particelle, in particolare cellule
WO2008104086A1 (en) 2007-03-01 2008-09-04 Queen's University At Kingston Planar electroporation apparatus and method
CN101038284B (zh) 2007-04-25 2011-04-27 博奥生物有限公司 一种提高电阻抗检测装置的电阻抗检测灵敏度的方法
US8673623B2 (en) * 2007-08-31 2014-03-18 Board Of Regents, The University Of Texas System Apparatus for performing magnetic electroporation
US20090087900A1 (en) * 2007-09-28 2009-04-02 Kent Davey Apparatus for Performing Electrodistention on Algae Cells
CN101556273B (zh) * 2008-04-08 2013-03-20 博奥生物有限公司 利用电阻抗传感技术分析细胞迁移的方法及其专用装置
US8008063B2 (en) * 2008-06-24 2011-08-30 Bio-Rad Laboratories, Inc. Individual-cell electroporation using area-focused electric fields
CN101614729B (zh) * 2008-06-27 2013-04-24 博奥生物有限公司 用于细胞操作及电生理信号检测的微电极阵列器件及专用装置
EP2147697A1 (de) * 2008-07-21 2010-01-27 Centre National De La Recherche Scientifique-CNRS Verfahren und Vorrichtung zur Anwendung elektrischer Felder in leitfähiges Material
US9304132B2 (en) 2009-04-16 2016-04-05 President And Fellows Of Harvard College Molecular delivery with nanowires
US20110065161A1 (en) * 2009-09-14 2011-03-17 Board Of Regents, The University Of Texas System Bipolar solid state marx generator
CN101870949B (zh) * 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 一种电穿孔芯片及基于电穿孔芯片的多孔板装置
ES2413557T3 (es) * 2010-06-22 2013-07-16 Lonza Cologne Gmbh Procedimiento y disposición de electrodos para tratar células adherentes
EP2621582A2 (de) * 2010-09-29 2013-08-07 President and Fellows of Harvard College Molekulare freisetzung mit nanodrähten
DK2940120T3 (en) * 2014-05-02 2018-11-26 Lonza Cologne Gmbh Device and method for large-volume transfection
US20180169403A1 (en) 2015-01-09 2018-06-21 President And Fellows Of Harvard College Nanowire arrays for neurotechnology and other applications
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
LU92920B1 (en) * 2015-12-21 2017-07-13 Luxembourg Inst Science & Tech List Electrochemical reactor to control the pH in miniaturized dimensions
CN114933967A (zh) * 2020-10-13 2022-08-23 北京航空航天大学 基于单细胞阵列的纳米电穿孔装置及其应用
CN113528332B (zh) * 2021-07-19 2023-10-24 中山大学 自动化电穿孔调控筛选的胞内外电生理记录系统与方法
JP7817391B2 (ja) * 2021-10-28 2026-02-18 ナノキャヴ，エルエルシー 細胞トランスフェクションのエレクトロポレーションデバイス及び方法
CN115120859B (zh) * 2022-05-18 2024-04-26 华南师范大学 一种高通量纳米电穿孔-纳米基因融合芯片及其制法和用途
CN116121063B (zh) * 2022-12-30 2023-08-04 山东大学 一种实现磁场调控与温度监测的生物芯片及其制备方法
CN118766470B (zh) * 2024-06-07 2026-02-17 中山大学 耦合微电穿孔的多尺寸微电极阵列检测装置及制备方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5134070A (en) * 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
US6352535B1 (en) * 1997-09-25 2002-03-05 Nanoptics, Inc. Method and a device for electro microsurgery in a physiological liquid environment
US6132683A (en) * 1998-12-23 2000-10-17 Matsushita Electric Industrial Co., Ltd. Cell potential measuring electrode and measuring apparatus using the same
DE19841337C1 (de) * 1998-05-27 1999-09-23 Micronas Intermetall Gmbh Verfahren und Vorrichtung zur intrazellulären Manipulation einer biologischen Zelle
CN1185492C (zh) * 1999-03-15 2005-01-19 清华大学 可单点选通式微电磁单元阵列芯片、电磁生物芯片及应用
US6300108B1 (en) * 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US20020090649A1 (en) * 1999-12-15 2002-07-11 Tony Chan High density column and row addressable electrode arrays
US7306924B2 (en) * 2000-04-17 2007-12-11 Purdue Research Foundation Biosensor and related method
US6846306B1 (en) 2000-10-10 2005-01-25 Cold Spring Harbor Laboratory Single cell electroporation
CA2493101C (en) * 2002-07-20 2012-05-08 Acea Biosciences, Inc. Impedance based apparatuses and methods for analyzing cells and particles
US7470533B2 (en) * 2002-12-20 2008-12-30 Acea Biosciences Impedance based devices and methods for use in assays
US7521224B2 (en) * 2003-09-30 2009-04-21 The United States Of America As Represented By The Secretary Of The Navy Microelectronic cell electroporation array

Also Published As

Publication number Publication date
CN1906305A (zh) 2007-01-31
US20070155015A1 (en) 2007-07-05
AU2004315392A1 (en) 2005-08-18
EP1720991B1 (de) 2010-01-13
JP2007519405A (ja) 2007-07-19
JP4588719B2 (ja) 2010-12-01
DK1720991T3 (da) 2010-05-25
CA2554027C (en) 2013-10-08
CN100497641C (zh) 2009-06-10
WO2005075656A8 (en) 2005-11-17
US8017367B2 (en) 2011-09-13
DE602004025146D1 (de) 2010-03-04
IL177110A0 (en) 2006-12-10
EP1720991A1 (de) 2006-11-15
CA2554027A1 (en) 2005-08-18
AU2004315392B2 (en) 2010-04-22
WO2005075656A1 (en) 2005-08-18

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