ATE456678T1 - Nukleinsäurenachweisverfahren mit erhöhter empfindlichkeit - Google Patents
Nukleinsäurenachweisverfahren mit erhöhter empfindlichkeitInfo
- Publication number
- ATE456678T1 ATE456678T1 AT04818676T AT04818676T ATE456678T1 AT E456678 T1 ATE456678 T1 AT E456678T1 AT 04818676 T AT04818676 T AT 04818676T AT 04818676 T AT04818676 T AT 04818676T AT E456678 T1 ATE456678 T1 AT E456678T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- detection method
- acid detection
- increased sensitivity
- technique involves
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 230000035945 sensitivity Effects 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000003992 Peroxidases Human genes 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000003197 catalytic effect Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000007899 nucleic acid hybridization Methods 0.000 abstract 1
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51881603P | 2003-11-10 | 2003-11-10 | |
| PCT/US2004/037407 WO2005047474A2 (en) | 2003-11-10 | 2004-11-10 | Nucleic acid detection method having increased sensitivity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE456678T1 true ATE456678T1 (de) | 2010-02-15 |
Family
ID=34590306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04818676T ATE456678T1 (de) | 2003-11-10 | 2004-11-10 | Nukleinsäurenachweisverfahren mit erhöhter empfindlichkeit |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20050186590A1 (de) |
| EP (2) | EP1687406B1 (de) |
| JP (1) | JP4947355B2 (de) |
| AT (1) | ATE456678T1 (de) |
| AU (1) | AU2004290036B2 (de) |
| CA (1) | CA2545356A1 (de) |
| DE (1) | DE602004025371D1 (de) |
| WO (1) | WO2005047474A2 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7258978B2 (en) * | 2002-11-06 | 2007-08-21 | Geneohm Sciences | Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen |
| WO2006065598A2 (en) * | 2004-12-13 | 2006-06-22 | Geneohm Sciences, Inc. | Fluidic cartridges for electrochemical detection of dna |
| US7910719B2 (en) * | 2005-12-08 | 2011-03-22 | Kabushiki Kaisha Toshiba | Method of detecting human papilloma virus by using nucleic acid amplification method and nucleic acid chain-immobilized carrier |
| JP2009540290A (ja) * | 2006-06-08 | 2009-11-19 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | Dna検出用ミクロ電子工学センサ装置 |
| DE102006027675B4 (de) * | 2006-06-14 | 2011-05-12 | Siemens Ag | Verfahren zur Bestimmung der Konzentration von Nukleinsäuren |
| US7948015B2 (en) | 2006-12-14 | 2011-05-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| EP2171097A2 (de) | 2007-06-29 | 2010-04-07 | Population Genetics Technologies LTD. | Verfahren und zusammensetzungen zur isolierung der varianten von nukleinsäuresequenzen |
| US9551026B2 (en) * | 2007-12-03 | 2017-01-24 | Complete Genomincs, Inc. | Method for nucleic acid detection using voltage enhancement |
| JP5712129B2 (ja) | 2008-09-02 | 2015-05-07 | ザ ガバニング カウンシル オブ ザ ユニバーシティ オブ トロント | ナノ構造化微小電極およびそれを組み込んだバイオセンシング装置 |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| JP2012506557A (ja) * | 2008-10-22 | 2012-03-15 | ライフ テクノロジーズ コーポレーション | 生物学的および化学的分析のための集積センサアレイ |
| WO2010060060A1 (en) * | 2008-11-24 | 2010-05-27 | Adnavance Technologies, Inc. | Electrochemical methods of detecting nucleic acid hybridization |
| US8776573B2 (en) | 2009-05-29 | 2014-07-15 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| AU2011226767B1 (en) | 2010-06-30 | 2011-11-10 | Life Technologies Corporation | Ion-sensing charge-accumulation circuits and methods |
| ES2704685T3 (es) | 2011-01-11 | 2019-03-19 | Governing Council Univ Toronto | Método para la detección de proteínas |
| US9580742B2 (en) | 2011-03-10 | 2017-02-28 | Shana O. Kelley | Diagnostic and sample preparation devices and methods |
| US10837879B2 (en) | 2011-11-02 | 2020-11-17 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
| US9970984B2 (en) | 2011-12-01 | 2018-05-15 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
| JP6206181B2 (ja) * | 2012-02-27 | 2017-10-04 | 東レ株式会社 | 核酸の検出方法 |
| WO2014076209A1 (en) * | 2012-11-14 | 2014-05-22 | Olink Ab | Localised rca-based amplification method |
| US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
| HK1226104A1 (zh) * | 2013-08-07 | 2017-09-22 | Xagenic, Inc. | 用辅助寡核苷酸电化学检测的系统、方法和装置 |
| EP3064934B1 (de) * | 2013-10-25 | 2020-06-03 | The Foundation for the Promotion of Industrial Science | Dna-nachweisverfahren |
| EP3234576B1 (de) | 2014-12-18 | 2023-11-22 | Life Technologies Corporation | Integrierte schaltung mit hoher datenrate und mit transmitterkonfiguration |
| GB201614023D0 (en) | 2016-08-16 | 2016-09-28 | Olink Bioscience Ab | Double-stranded circle probes |
| CN112400016A (zh) * | 2018-06-26 | 2021-02-23 | Tdk株式会社 | 用于检测dna的试剂盒与方法 |
| JP7333928B2 (ja) * | 2018-10-05 | 2023-08-28 | 国立大学法人山形大学 | 電気化学式非標識核酸検出の測定方法 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3310337A1 (de) * | 1983-03-22 | 1984-09-27 | Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg | Verfahren zur durchfuehrung von hybridisierungsreaktionen |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| WO1985002627A1 (en) * | 1983-12-16 | 1985-06-20 | Genetics International, Inc. | Assay for nucleic acids |
| US5019496A (en) * | 1989-02-17 | 1991-05-28 | Gerald Oster | Photopolymerization diagnostic test composition and method for immunoassay and nucleic acid assay |
| US5776672A (en) * | 1990-09-28 | 1998-07-07 | Kabushiki Kaisha Toshiba | Gene detection method |
| US5372931A (en) * | 1992-12-22 | 1994-12-13 | Eastman Kodak Company | Use of 4'-hydroxy- and 4'-alkoxy-substituted electron transfer agents in compositions, elements, test kits and analytical methods |
| US6638482B1 (en) * | 1993-11-01 | 2003-10-28 | Nanogen, Inc. | Reconfigurable detection and analysis apparatus and method |
| US5695934A (en) * | 1994-10-13 | 1997-12-09 | Lynx Therapeutics, Inc. | Massively parallel sequencing of sorted polynucleotides |
| US5604097A (en) * | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US5846719A (en) * | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US6458530B1 (en) * | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
| CA2276462C (en) * | 1996-12-31 | 2007-06-12 | High Throughput Genomics, Inc. | Multiplexed molecular analysis system apparatus and method |
| US6391558B1 (en) * | 1997-03-18 | 2002-05-21 | Andcare, Inc. | Electrochemical detection of nucleic acid sequences |
| EP1985714B1 (de) * | 1998-03-25 | 2012-02-29 | Olink AB | Zwei Affinitätsproben und Rolling-Circle-Replikation zur Detektion eines Zielmoleküls verwendende Verfahren und Kit |
| US6290839B1 (en) * | 1998-06-23 | 2001-09-18 | Clinical Micro Sensors, Inc. | Systems for electrophoretic transport and detection of analytes |
| WO2000004193A1 (en) * | 1998-07-20 | 2000-01-27 | Yale University | Method for detecting nucleic acids using target-mediated ligation of bipartite primers |
| US6638716B2 (en) * | 1998-08-24 | 2003-10-28 | Therasense, Inc. | Rapid amperometric verification of PCR amplification of DNA |
| CA2367400A1 (en) * | 1999-04-06 | 2000-10-12 | Yale University | Fixed address analysis of sequence tags |
| JP2003527087A (ja) * | 1999-08-13 | 2003-09-16 | イェール・ユニバーシティ | バイナリーコード化配列タグ |
| US6448004B1 (en) * | 1999-10-05 | 2002-09-10 | Aventis Pharmaceuticals Inc. | Electrochemiluminescence helicase assay |
| FR2805545B1 (fr) * | 2000-02-24 | 2002-05-17 | Argene Sa | Procede electrochimique de detection d'acides nucleiques |
| US6291187B1 (en) * | 2000-05-12 | 2001-09-18 | Molecular Staging, Inc. | Poly-primed amplification of nucleic acid sequences |
| IL138229A (en) * | 2000-09-04 | 2005-03-20 | Yissum Res Dev Co | Method and system for detecting nucleic acids |
| AU2003303395A1 (en) * | 2001-05-22 | 2004-07-22 | Dahl, Gary, A. | Target-dependent transcription using deletion mutants of n4 rna polymerase |
| US7468244B2 (en) * | 2001-09-27 | 2008-12-23 | University Of Delaware | Polymorphism detection and separation |
| WO2003064692A1 (en) * | 2002-01-29 | 2003-08-07 | Atlantic Biolabs, Inc. | Detecting and quantifying many target nucleic acids within a single sample |
| EP1478783A4 (de) * | 2002-02-06 | 2006-12-27 | Ge Healthcare Bio Sciences Ab | Zusammensetzungen und verfahren für rolling circle-amplifikation |
| US20050118616A1 (en) * | 2002-08-16 | 2005-06-02 | Kawashima Tadashi R. | Amplification of target nucleotide sequence without polymerase chain reaction |
| US20040086892A1 (en) * | 2002-11-06 | 2004-05-06 | Crothers Donald M. | Universal tag assay |
| US7258978B2 (en) * | 2002-11-06 | 2007-08-21 | Geneohm Sciences | Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen |
| US20040086895A1 (en) * | 2002-11-06 | 2004-05-06 | Crothers Donald M. | Method of electrochemical detection of somatic cell mutations |
| US7531306B2 (en) * | 2003-11-12 | 2009-05-12 | Geneohm Sciences, Inc. | Nucleic acid hybridization methods |
-
2004
- 2004-11-10 EP EP04818676A patent/EP1687406B1/de not_active Expired - Lifetime
- 2004-11-10 EP EP09015418A patent/EP2163653B1/de not_active Expired - Lifetime
- 2004-11-10 WO PCT/US2004/037407 patent/WO2005047474A2/en not_active Ceased
- 2004-11-10 AT AT04818676T patent/ATE456678T1/de not_active IP Right Cessation
- 2004-11-10 JP JP2006539763A patent/JP4947355B2/ja not_active Expired - Fee Related
- 2004-11-10 AU AU2004290036A patent/AU2004290036B2/en not_active Ceased
- 2004-11-10 US US10/985,256 patent/US20050186590A1/en not_active Abandoned
- 2004-11-10 CA CA002545356A patent/CA2545356A1/en not_active Abandoned
- 2004-11-10 DE DE602004025371T patent/DE602004025371D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005047474A3 (en) | 2006-09-14 |
| AU2004290036B2 (en) | 2011-08-04 |
| JP2007512810A (ja) | 2007-05-24 |
| JP4947355B2 (ja) | 2012-06-06 |
| EP2163653A2 (de) | 2010-03-17 |
| EP2163653A3 (de) | 2010-05-26 |
| EP1687406A4 (de) | 2008-05-21 |
| EP2163653B1 (de) | 2013-03-27 |
| EP1687406B1 (de) | 2010-01-27 |
| EP1687406A2 (de) | 2006-08-09 |
| WO2005047474A2 (en) | 2005-05-26 |
| CA2545356A1 (en) | 2005-05-26 |
| US20050186590A1 (en) | 2005-08-25 |
| DE602004025371D1 (de) | 2010-03-18 |
| AU2004290036A1 (en) | 2005-05-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |