ATE461292T1 - CREATION OF EXPRESSION PROFILES USING MICROARRAYS - Google Patents

CREATION OF EXPRESSION PROFILES USING MICROARRAYS

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Publication number
ATE461292T1
ATE461292T1 AT04816864T AT04816864T ATE461292T1 AT E461292 T1 ATE461292 T1 AT E461292T1 AT 04816864 T AT04816864 T AT 04816864T AT 04816864 T AT04816864 T AT 04816864T AT E461292 T1 ATE461292 T1 AT E461292T1
Authority
AT
Austria
Prior art keywords
methods
microarrays
creation
microarray
expression profiles
Prior art date
Application number
AT04816864T
Other languages
German (de)
Inventor
Joseph Monforte
Original Assignee
Althea Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Althea Technologies Inc filed Critical Althea Technologies Inc
Application granted granted Critical
Publication of ATE461292T1 publication Critical patent/ATE461292T1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides novel compositions and methods for the analysis of gene expression (e.g., expression profiling) using microarray-based technology. In some embodiments of the invention, the novel methods use gene-specific as well as universal amplification primers during sample preparation, and the methods permit the simultaneous analysis of multiple samples on the same microarray. Furthermore, some embodiments of the invention incorporate barcode sequences into the amplified products, thereby permitting the use of generic arrays and generic labeled probes.
AT04816864T 2003-09-10 2004-09-10 CREATION OF EXPRESSION PROFILES USING MICROARRAYS ATE461292T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50210803P 2003-09-10 2003-09-10
US53828304P 2004-01-21 2004-01-21
PCT/US2004/029749 WO2005042759A2 (en) 2003-09-10 2004-09-10 Expression profiling using microarrays

Publications (1)

Publication Number Publication Date
ATE461292T1 true ATE461292T1 (en) 2010-04-15

Family

ID=34555682

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04816864T ATE461292T1 (en) 2003-09-10 2004-09-10 CREATION OF EXPRESSION PROFILES USING MICROARRAYS

Country Status (9)

Country Link
US (2) US7824856B2 (en)
EP (1) EP1660674B1 (en)
JP (1) JP5171037B2 (en)
AT (1) ATE461292T1 (en)
AU (1) AU2004286201B2 (en)
CA (1) CA2536565A1 (en)
DE (1) DE602004026077D1 (en)
SG (1) SG149007A1 (en)
WO (1) WO2005042759A2 (en)

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