ATE46363T1 - Klonierungs- und expressionsplasmidvektoren fuer ein protein in einem mikroorganismus die mindestens den expressionspromotor von betaglucosidase in den hefen enthalten, dieselben enthaltende mikroorganismen, gaerungsverfahren und erhaltene enzyme. - Google Patents

Klonierungs- und expressionsplasmidvektoren fuer ein protein in einem mikroorganismus die mindestens den expressionspromotor von betaglucosidase in den hefen enthalten, dieselben enthaltende mikroorganismen, gaerungsverfahren und erhaltene enzyme.

Info

Publication number
ATE46363T1
ATE46363T1 AT84402482T AT84402482T ATE46363T1 AT E46363 T1 ATE46363 T1 AT E46363T1 AT 84402482 T AT84402482 T AT 84402482T AT 84402482 T AT84402482 T AT 84402482T AT E46363 T1 ATE46363 T1 AT E46363T1
Authority
AT
Austria
Prior art keywords
expression
protein
glucosidase
beta
yeasts
Prior art date
Application number
AT84402482T
Other languages
English (en)
Inventor
Michel Guerineau
Alain Raynal
Original Assignee
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient filed Critical Centre Nat Rech Scient
Application granted granted Critical
Publication of ATE46363T1 publication Critical patent/ATE46363T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/94—Saccharomyces
    • Y10S435/942—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT84402482T 1983-12-06 1984-12-04 Klonierungs- und expressionsplasmidvektoren fuer ein protein in einem mikroorganismus die mindestens den expressionspromotor von betaglucosidase in den hefen enthalten, dieselben enthaltende mikroorganismen, gaerungsverfahren und erhaltene enzyme. ATE46363T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8319494A FR2556008B1 (fr) 1983-12-06 1983-12-06 Vecteurs plasmidiques de clonage et d'expression d'une proteine dans un micro-organisme, comportant au moins le promoteur d'expression de la b-glucosidase dans les levures; micro-organismes les contenant; procede de fermentation et enzymes obtenues
EP84402482A EP0148668B1 (de) 1983-12-06 1984-12-04 Klonierungs- und Expressionsplasmidvektoren für ein Protein in einem Mikroorganismus die mindestens den Expressionspromotor von beta-Glucosidase in den Hefen enthalten, dieselben enthaltende Mikroorganismen, Gärungsverfahren und erhaltene Enzyme

Publications (1)

Publication Number Publication Date
ATE46363T1 true ATE46363T1 (de) 1989-09-15

Family

ID=9294899

Family Applications (1)

Application Number Title Priority Date Filing Date
AT84402482T ATE46363T1 (de) 1983-12-06 1984-12-04 Klonierungs- und expressionsplasmidvektoren fuer ein protein in einem mikroorganismus die mindestens den expressionspromotor von betaglucosidase in den hefen enthalten, dieselben enthaltende mikroorganismen, gaerungsverfahren und erhaltene enzyme.

Country Status (7)

Country Link
US (1) US4745062A (de)
EP (1) EP0148668B1 (de)
JP (1) JPS60141292A (de)
AT (1) ATE46363T1 (de)
BR (1) BR8406215A (de)
DE (1) DE3479745D1 (de)
FR (1) FR2556008B1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0668B2 (ja) * 1985-08-30 1994-01-05 財団法人化学及血清療法研究所 単純ヘルペスウイルス遺伝子が組込まれた組換えプラスミド
JPS62181775A (ja) * 1986-02-05 1987-08-10 Sanyo Kokusaku Pulp Co Ltd 分泌型β−グルコシダ−ゼ遺伝子を有する酵母の製造方法
US5298405A (en) * 1986-04-30 1994-03-29 Alko Limited Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production
DE3804891A1 (de) * 1988-01-05 1989-07-13 Boehringer Mannheim Gmbh Expressionsvektor und verfahren zur regulierbaren herstellung von proteinen in eukaryonten
US6103464A (en) * 1990-12-10 2000-08-15 Genencor International, Inc. Method of detecting DNA encoding a β-glucosidase from a filamentous fungus
ATE223490T1 (de) 1990-12-10 2002-09-15 Genencor Int Verbesserte saccharifizierung von zellulose durch klonierung und vervielfältigung des beta- glukosidase genes aus trichoderma reesei
FR2706485B1 (fr) * 1993-06-15 1995-09-01 Rhone Poulenc Rorer Sa Promoteur de levure et son utilisation.
DK0684306T4 (da) * 1994-05-27 2004-05-10 Agrano Ag Fremgangsmåde til fremstilling af en biomasse ud fra et kornsubstrat, anvendelse af de ved fremgangsmåden opnåede produkter, samt brödbagningsgærstof
DK0684308T4 (da) * 1994-05-27 2004-05-10 Agrano Ag Fremgangsmåde til fremstilling af en biomasse, anvendelse af den ved fremgangsmåden opnåede biomasse samt brödbagningsgærstof
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275163A (en) * 1978-11-20 1981-06-23 The United States Of America As Represented By The Secretary Of The Army Cellulase-producing microorganism
US4243752A (en) * 1979-04-09 1981-01-06 Sri International Production of increased yields of cellulolytic enzymes from Thielavia terrestris and separating methods therefor
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
FR2489363A1 (fr) * 1980-08-29 1982-03-05 Pasteur Institut Nouveaux plasmides hybrides utiles notamment pour l'introduction des genes responsables de la fixation de l'azote dans des microorganismes et cellules eucaryotes; microorganismes et cellules eucaryotes contenant lesdits plasmides
US4464471A (en) * 1982-02-01 1984-08-07 University Of Cincinnati Biologically engineered plasmid coding for production of β-glucosidase, organisms modified by this plasmid and methods of use
US4487831A (en) * 1982-05-19 1984-12-11 Research Corporation Process for the conversion of cellulose to glucose

Also Published As

Publication number Publication date
FR2556008B1 (fr) 1987-06-26
EP0148668B1 (de) 1989-09-13
BR8406215A (pt) 1985-10-01
FR2556008A1 (fr) 1985-06-07
EP0148668A2 (de) 1985-07-17
JPS60141292A (ja) 1985-07-26
EP0148668A3 (en) 1985-08-28
DE3479745D1 (en) 1989-10-19
US4745062A (en) 1988-05-17

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