ATE466096T1 - Zellisolierungsverfahren und verwendungen davon - Google Patents

Zellisolierungsverfahren und verwendungen davon

Info

Publication number
ATE466096T1
ATE466096T1 AT02728540T AT02728540T ATE466096T1 AT E466096 T1 ATE466096 T1 AT E466096T1 AT 02728540 T AT02728540 T AT 02728540T AT 02728540 T AT02728540 T AT 02728540T AT E466096 T1 ATE466096 T1 AT E466096T1
Authority
AT
Austria
Prior art keywords
cells
separating
differentially stained
property
stained cells
Prior art date
Application number
AT02728540T
Other languages
English (en)
Inventor
Gaoshan Jing
Jian Zhang
Jing Cheng
Original Assignee
Capitalbio Corp
Univ Tsinghua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Capitalbio Corp, Univ Tsinghua filed Critical Capitalbio Corp
Application granted granted Critical
Publication of ATE466096T1 publication Critical patent/ATE466096T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Electrostatic Separation (AREA)
  • Centrifugal Separators (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Local Oxidation Of Silicon (AREA)
  • Element Separation (AREA)
AT02728540T 2001-03-22 2002-03-20 Zellisolierungsverfahren und verwendungen davon ATE466096T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CNB01110015XA CN100494360C (zh) 2001-03-22 2001-03-22 细胞分离方法及其应用
PCT/US2002/008880 WO2002077269A1 (en) 2001-03-22 2002-03-20 Cell isolation method and uses thereof

Publications (1)

Publication Number Publication Date
ATE466096T1 true ATE466096T1 (de) 2010-05-15

Family

ID=4658284

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02728540T ATE466096T1 (de) 2001-03-22 2002-03-20 Zellisolierungsverfahren und verwendungen davon

Country Status (8)

Country Link
US (2) US7153648B2 (de)
EP (2) EP1379682B1 (de)
JP (2) JP2004522452A (de)
CN (1) CN100494360C (de)
AT (1) ATE466096T1 (de)
CA (1) CA2440385C (de)
DE (1) DE60236145D1 (de)
WO (1) WO2002077269A1 (de)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8980568B2 (en) 2001-10-11 2015-03-17 Aviva Biosciences Corporation Methods and compositions for detecting non-hematopoietic cells from a blood sample
US8986944B2 (en) * 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
US7998699B2 (en) 2002-08-15 2011-08-16 University Of South Florida Early detection of pathogens in blood
ITTO20030039A1 (it) * 2003-01-24 2004-07-25 Fresenius Hemocare Italia Srl Filtro per separare leucociti da sangue intero e/o da preparati derivati dal sangue, procedimento per la fabbricazione del filtro, dispositivo e utilizzazione.
PT2103694E (pt) 2003-08-15 2011-07-26 Univ South Florida Materiais e métodos para a captura de patogénios e a remoção de ácido aurintricarboxílico de uma amostra
US7384791B2 (en) * 2004-01-21 2008-06-10 Hewlett-Packard Development Company, L.P. Method of analyzing blood
US7390387B2 (en) * 2004-03-25 2008-06-24 Hewlett-Packard Development Company, L.P. Method of sorting cells in series
US7160425B2 (en) * 2004-03-25 2007-01-09 Hewlett-Packard Development Company, L.P. Cell transporter for a biodevice
US7390388B2 (en) * 2004-03-25 2008-06-24 Hewlett-Packard Development Company, L.P. Method of sorting cells on a biodevice
FR2876045B1 (fr) * 2004-10-04 2006-11-10 Commissariat Energie Atomique Dispositif pour realiser la separation dielectrophoretique de particules contenues dans un fluide
US20060177815A1 (en) * 2004-11-29 2006-08-10 The Regents Of The University Of California Dielectrophoretic particle sorter
KR100738071B1 (ko) * 2005-01-21 2007-07-12 삼성전자주식회사 농도구배발생부가 구비된 유전영동 장치, 그를 이용한물질의 분리방법 및 물질 분리의 최적 조건을 탐색하는 방법
JP2009014342A (ja) * 2005-10-19 2009-01-22 Sharp Corp 誘電泳動チップおよび誘電泳動装置並びに誘電泳動システム
WO2007046484A1 (ja) * 2005-10-19 2007-04-26 Sharp Kabushiki Kaisha 誘電泳動チップおよび誘電泳動装置並びに誘電泳動システム
JP4300296B2 (ja) 2006-02-10 2009-07-22 学校法人高知工科大学 粒子状物質の角度変調波による誘電泳動を利用した特性分析装置並びに方法
CA2657621A1 (en) 2006-07-14 2008-01-17 Aviva Biosciences Corporation Methods and compositions for detecting rare cells from a biological sample
CN101177673B (zh) * 2006-11-06 2010-05-12 瑞鼎科技股份有限公司 排列细胞的方法及其使用的电极图案
ITTO20070307A1 (it) 2007-05-04 2008-11-05 Silicon Biosystems Spa Metodo e dispositivo per la diagnosi prenatale non-invasiva
WO2009044902A1 (ja) * 2007-10-05 2009-04-09 Kyushu Institute Of Technology 誘電泳動装置および方法
US8162149B1 (en) 2009-01-21 2012-04-24 Sandia Corporation Particle sorter comprising a fluid displacer in a closed-loop fluid circuit
US9134221B2 (en) 2009-03-10 2015-09-15 The Regents Of The University Of California Fluidic flow cytometry devices and particle sensing based on signal-encoding
EP2511011A4 (de) 2009-12-07 2014-07-09 Min-Yong Jeon Zentrifugenrohr
JP5611582B2 (ja) * 2009-12-25 2014-10-22 株式会社東芝 電気的中性物質の分離方法、及び電気的中性物質の分離装置
EP2526183A4 (de) 2010-01-21 2017-05-03 Biocep Ltd. Magnetische trennung seltener zellen
US8774488B2 (en) 2010-03-11 2014-07-08 Cellscape Corporation Method and device for identification of nucleated red blood cells from a maternal blood sample
US20110225809A1 (en) * 2010-03-17 2011-09-22 Alan Francis Daher Apparatus for removably attaching an item to a surface
JP5771917B2 (ja) * 2010-08-04 2015-09-02 公益財団法人ヒューマンサイエンス振興財団 単核球分離管及び単核球分離システム
WO2012054904A2 (en) * 2010-10-21 2012-04-26 The Regents Of The University Of California Microfluidics with wirelessly powered electronic circuits
KR101284725B1 (ko) * 2011-07-22 2013-07-17 한국항공대학교산학협력단 유전영동력을 이용한 고속 입자분리 시스템
CA2845713A1 (en) * 2011-08-19 2013-02-28 David Martin Morrow Gradient array dielectrophoresis separation (grads) with concomitant light therapy
EP2749882B1 (de) * 2011-08-24 2017-05-03 Eiken Kagaku Kabushiki Kaisha Leukozytenmessvorrichtung und reagenzienkit
US20140207239A1 (en) 2011-09-09 2014-07-24 Spine Wave, Inc. Lateral approach expandable spinal implant and method
US9120105B2 (en) 2011-10-31 2015-09-01 Monika Weber Electronic device for pathogen detection
US11198126B2 (en) 2011-10-31 2021-12-14 Fluid-Screen, Inc. Apparatus for pathogen detection
US8926816B2 (en) 2011-11-08 2015-01-06 Rarecyte, Inc. Systems and methods to analyze materials of a suspension by means of dielectrophoresis
TWI448678B (zh) * 2012-03-23 2014-08-11 Univ Nat Cheng Kung 液體樣本之帶電粒子的分離方法及裝置與該裝置的製作方法
US10564147B2 (en) 2012-05-25 2020-02-18 The Regents Of The University Of California Microfluidic systems for particle trapping and separation using cavity acoustic transducers
TW201413230A (zh) * 2012-09-21 2014-04-01 Nat Applied Res Laboratories 可選擇地濃縮分離待測粒子的方法與晶片
JP5990440B2 (ja) * 2012-09-26 2016-09-14 テルモ株式会社 ヘマトクリット値の測定方法
EP2906928A4 (de) 2012-10-15 2016-11-09 Nanocellect Biomedical Inc Systeme, vorrichtungen und verfahren zum sortieren von partikeln
MX365324B (es) * 2013-03-15 2019-05-29 Theranos Ip Co Llc Metodos y dispositivos para recoleccion de muestras y separacion de muestras.
WO2015031586A1 (en) * 2013-08-29 2015-03-05 Apocell, Inc. Method and apparatus for isolation, capture and molecular analysis of target particles
WO2016045022A1 (zh) * 2014-09-25 2016-03-31 深圳华大基因科技有限公司 用于孕妇外周血样的保存液和孕妇外周血样的保存方法
CN106513179B (zh) * 2015-09-15 2019-05-31 王冰 一种钣金介电电泳电极结构
WO2017061496A1 (ja) * 2015-10-07 2017-04-13 株式会社Ａｆｉテクノロジー 検査装置、検査システム、及び検査方法
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
US10549277B2 (en) 2015-10-14 2020-02-04 The Regents Of The University Of California Integrated microfluidic platform for selective extraction of single-cell mRNA
SG11201808959QA (en) 2016-04-15 2018-11-29 Fluid Screen Inc Analyte detection methods and apparatus using dielectrophoresis and electroosmosis
CN107881104B (zh) * 2016-09-30 2023-04-14 国立大学法人东京大学 粒子捕获用微装置以及使用它的粒子的捕获、浓缩或分离方法
US11090653B2 (en) 2016-10-11 2021-08-17 The Regents Of The University Of California Systems and methods to encapsulate and preserve organic matter for analysis
US20190351409A1 (en) 2016-12-29 2019-11-21 Ador Diagnostics S.R.L. Cartridge for use in in-vitro diagnostics and method of use thereof
WO2018227210A1 (en) 2017-06-09 2018-12-13 The Regents Of The University Of California High-efficiency encapsulation in droplets based on hydrodynamic vortices control
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
WO2019079787A1 (en) 2017-10-20 2019-04-25 The Regents Of The University Of California MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX
WO2019147278A1 (en) 2018-01-29 2019-08-01 Hewlett-Packard Development Company, L.P. Object separating
US12179199B2 (en) 2019-08-09 2024-12-31 The Regents Of The University Of California Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments
EP4058778A4 (de) 2019-11-13 2023-12-27 Fluid-Screen, Inc. Verfahren und vorrichtung zum nachweis von bakterien in einer probe mittels dielektrophorese
EP4058055A4 (de) 2019-11-13 2023-12-13 Fluid-Screen, Inc. Vorrichtung und verfahren zur schnellen detektion, trennung, reinigung und quantifizierung verschiedener viren aus zellen, kulturmedien und anderen flüssigkeiten
CN110777060A (zh) * 2019-11-14 2020-02-11 北京酷搏科技有限公司 反应管、反应管阵列、控制参与反应的样品体积的方法及其应用
CN112362711B (zh) * 2020-11-11 2021-08-17 重庆大学 一种微生物检测装置及检测方法
CN112871229B (zh) * 2021-01-21 2022-06-28 中国科学技术大学 一种用于水体介电泳细菌分选的芯片
WO2024036174A1 (en) * 2022-08-08 2024-02-15 Sigil Biosciences, Inc. Systems and methods for isolating particles in solution by particle permittivity

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3975156A (en) * 1975-11-10 1976-08-17 Ortho Diagnostics, Inc. Method and material for detecting and quantitating fetal erythrocytes in adults
US4326934A (en) * 1979-12-31 1982-04-27 Pohl Herbert A Continuous dielectrophoretic cell classification method
US4786387A (en) * 1986-09-25 1988-11-22 Whitlock David R Single phase enrichment of super critical fluids
US5641628A (en) 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
GB9301122D0 (en) * 1993-01-21 1993-03-10 Scient Generics Ltd Method of analysis/separation
GB9306729D0 (en) * 1993-03-31 1993-05-26 British Tech Group Improvements in separators
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US6071394A (en) * 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US5662813A (en) 1994-10-21 1997-09-02 Bioseparations, Inc. Method for separation of nucleated fetal erythrocytes from maternal blood samples
DE19603043C2 (de) * 1996-01-29 1997-11-27 Ibm Ionenerzeuger für ionographischen Druckkopf und Verfahren zu dessen Herstellung
US5993630A (en) * 1996-01-31 1999-11-30 Board Of Regents The University Of Texas System Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation
US5888370A (en) * 1996-02-23 1999-03-30 Board Of Regents, The University Of Texas System Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation
EP0885055B1 (de) * 1996-01-31 2007-04-04 The Board Of Regents, The University Of Texas System Fraktionierung mittels dielektrophoresis und fraktionierung unter anwendung eines flussfeldes
US5866071A (en) 1996-03-06 1999-02-02 National Science Council Centrifuge tube with a built-in small tubing for separation following density gradients centrifugation
EP0925494B1 (de) * 1996-09-04 2001-12-19 Scandinavian Micro Biodevices A/S Mikrofliesssystem für die teilchenanalyse und trennung
DE19815882A1 (de) 1998-04-08 1999-10-14 Fuhr Guenther Verfahren und Vorrichtung zur Manipulierung von Mikropartikeln in Fluidströmungen
CA2333201A1 (en) * 1998-05-22 1999-12-02 California Institute Of Technology Microfabricated cell sorter
WO1999062622A1 (en) * 1998-05-29 1999-12-09 Industrial Research Limited Method and apparatus for concentrating and/or positioning particles or cells
JP2002008115A (ja) * 2000-06-23 2002-01-11 Sony Corp 情報配信システム、端末装置、サーバ装置、記録媒体、情報配信方法
AU2001285219A1 (en) 2000-08-24 2002-03-04 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
CN1325909C (zh) * 2000-09-27 2007-07-11 清华大学 用于微粒操纵与微粒导向的装置及其使用方法
EP1320922A2 (de) * 2000-09-27 2003-06-25 Aviva Biosciences Corporation Apparat zur umschalten und behandlen von teilchen und benutzungsverfahren dieses apparates
CN100495030C (zh) * 2000-09-30 2009-06-03 清华大学 多力操纵装置及其应用
CA2421828A1 (en) 2000-09-30 2002-04-11 Xiao-Bo Wang Apparatuses containing multiple force generating elements and uses thereof
CA2422837A1 (en) 2000-09-30 2002-04-11 Aviva Biosciences Corporation Apparatuses and methods for field flow fractionation of particles using acoustic and other forces

Also Published As

Publication number Publication date
US7153648B2 (en) 2006-12-26
EP1379682A4 (de) 2005-08-10
US20070128686A1 (en) 2007-06-07
CN1376779A (zh) 2002-10-30
WO2002077269A1 (en) 2002-10-03
DE60236145D1 (de) 2010-06-10
EP2116609A2 (de) 2009-11-11
CA2440385C (en) 2012-01-10
JP4411266B2 (ja) 2010-02-10
CA2440385A1 (en) 2002-10-03
EP2116609B1 (de) 2014-02-12
EP1379682B1 (de) 2010-04-28
EP2116609A3 (de) 2010-01-20
US20020182654A1 (en) 2002-12-05
JP2004522452A (ja) 2004-07-29
US7918981B2 (en) 2011-04-05
EP1379682A1 (de) 2004-01-14
JP2006126195A (ja) 2006-05-18
CN100494360C (zh) 2009-06-03

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