ATE466107T1 - Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe - Google Patents

Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe

Info

Publication number
ATE466107T1
ATE466107T1 AT05767727T AT05767727T ATE466107T1 AT E466107 T1 ATE466107 T1 AT E466107T1 AT 05767727 T AT05767727 T AT 05767727T AT 05767727 T AT05767727 T AT 05767727T AT E466107 T1 ATE466107 T1 AT E466107T1
Authority
AT
Austria
Prior art keywords
capture
nucleic acid
hairpin
methods
sample
Prior art date
Application number
AT05767727T
Other languages
English (en)
Inventor
Michael Becker
Mehrdad Majlessi
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE466107T1 publication Critical patent/ATE466107T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT05767727T 2004-07-01 2005-06-29 Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe ATE466107T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58542104P 2004-07-01 2004-07-01
PCT/US2005/023555 WO2006007567A2 (en) 2004-07-01 2005-06-29 Methods and compositions to detect nucleic acids in a biological sample

Publications (1)

Publication Number Publication Date
ATE466107T1 true ATE466107T1 (de) 2010-05-15

Family

ID=35457287

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05767727T ATE466107T1 (de) 2004-07-01 2005-06-29 Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe

Country Status (9)

Country Link
US (3) US8034554B2 (de)
EP (1) EP1778867B1 (de)
JP (1) JP5020818B2 (de)
AT (1) ATE466107T1 (de)
AU (2) AU2005262357B2 (de)
CA (1) CA2572472C (de)
DE (1) DE602005020943D1 (de)
DK (1) DK1778867T3 (de)
WO (1) WO2006007567A2 (de)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006007567A2 (en) 2004-07-01 2006-01-19 Gen-Probe Incorporated Methods and compositions to detect nucleic acids in a biological sample
DE602005022759D1 (de) * 2004-12-08 2010-09-16 Gen Probe Inc Nukleinsäuredetektion aus verschiedenen typen des humanen papillomavirus
US7993853B2 (en) * 2005-05-06 2011-08-09 Gen-Probe Incorporated Methods of nucleic acid target capture
US8632970B2 (en) * 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
WO2007035685A2 (en) * 2005-09-16 2007-03-29 Primera Biosystems, Inc. Compositions and methods for purifying nucleic acids
AU2006304721B2 (en) 2005-10-17 2012-01-19 Gen-Probe Incorporated Compositions and methods to detect Legionella pneumophila nucleic acid
JP5777269B2 (ja) 2006-02-13 2015-09-09 フリューダイム カナダ インコーポレイテッド 元素分析によって実施される遺伝子発現アッセイ
CA2651946A1 (en) * 2006-05-12 2007-11-22 Gen-Probe Incorporated Compositions and methods to detect enterococci nucleic acid
DE602007012045D1 (de) 2006-06-06 2011-03-03 Gen Probe Inc Markierte oligonukleotide und ihre verwendung in nukleinsäureverstärkungsverfahren
WO2008052774A2 (en) * 2006-10-31 2008-05-08 Noxxon Pharma Ag Methods for detection of a single- or double-stranded nucleic acid molecule
AU2014200826B2 (en) * 2006-11-06 2016-01-14 Clondiag Gmbh Assays
CN101553306B (zh) * 2006-11-06 2013-04-03 科隆迪亚戈有限公司 使用结合元件用于分析的装置和方法
AU2016202243B2 (en) * 2006-11-06 2017-12-14 Clondiag Gmbh Assays
WO2008080029A2 (en) 2006-12-21 2008-07-03 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP1978111B1 (de) 2007-04-02 2013-03-27 Gen-Probe Incorporated Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa
WO2009013321A2 (en) 2007-07-23 2009-01-29 Clondiag Gmbh Assays
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
EP2235214B1 (de) * 2007-12-26 2014-07-09 Gen Probe Inc Zusammensetzungen und verfahren für den nachweis von candida albicans-nukleinsäure
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
EP2806037B1 (de) 2008-05-13 2016-09-21 Gen-Probe Incorporated Inaktivierbare Target-Capture-Oligomere zur Verwendung bei der selektiven Hybridisierung und beim Fangen von Zielnukleinsäuresequenzen
CA2723917A1 (en) * 2008-05-30 2009-12-30 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of salmonella
WO2010009398A1 (en) * 2008-07-18 2010-01-21 Xenomics, Inc. Methods for pcr-based detection of "ultra short" nucleic acid sequences
AU2009324884B2 (en) * 2008-11-25 2013-10-03 Gen-Probe Incorporated Compositions and methods for detecting small RNAs, and uses thereof
EP2389449B1 (de) 2008-12-30 2015-02-18 Gen-Probe Incorporated Zusammensetzungen, kits und damit verbundene verfahren zum nachweis und/oder überwachen von listerien
EP2446057B1 (de) 2009-06-23 2014-11-19 Gen-Probe Incorporated Zusammensetzungen und verfahren für den nukleinsäurenachweis aus mollicutes
CA2766391C (en) 2009-07-01 2021-04-20 Gen-Probe Incorporated Methods and compositions for multiplex nucleic acid amplification
WO2011026139A1 (en) 2009-08-31 2011-03-03 Gen-Probe Incorporated Dengue virus assay
EP3604558B1 (de) 2010-02-17 2022-10-12 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von atopobium-vaginae-nukleinsäure
EP3587593B1 (de) 2010-04-21 2023-09-27 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits für den nachweis von nukleinsäuren des herpes-simplex-virus
WO2012030856A2 (en) 2010-08-30 2012-03-08 Gen-Probe Incorporated Compositions, methods and reaction mixtures for the detection of xenotropic murine leukemia virus-related virus
EP2616555B1 (de) * 2010-09-16 2017-11-08 Gen-Probe Incorporated Über l-nukleotidenden immobilisierbare fängersonden
EP3438289A1 (de) 2010-10-04 2019-02-06 Gen-Probe Prodesse, Inc. Zusammensetzungen, verfahren und kits für den nachweis von adenovirus-nukleinsäuren
AU2011323478B2 (en) 2010-11-01 2015-09-10 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
USRE49975E1 (en) 2011-03-10 2024-05-21 Gen-Probe Incorporated Methods and compositions for the selection and optimization of oligonucleotide tag sequences
EP3604559A1 (de) 2011-04-25 2020-02-05 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von bv-assoziierter bakterieller nukleinsäure
CA3113828A1 (en) 2011-07-15 2013-01-24 Gen-Probe Incorporated Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis a virus nucleic acids in single-plex or multiplex assays
EP4219741A3 (de) 2011-09-06 2023-08-23 Gen-Probe Incorporated Geschlossene nukleinsäurestrukturen
EP3255155B1 (de) 2011-09-08 2019-04-24 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von bv-assoziierter bakterieller nukleinsäure
US9175337B2 (en) 2011-09-21 2015-11-03 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
DE102011120550B4 (de) 2011-12-05 2013-11-07 Gen-Probe Prodesse, Inc. Zusammensetzungen, Verfahren und Kits zur Detektion von Adenovirusnukleinsäuren
EP2809807B1 (de) * 2012-02-01 2019-04-10 Gen-Probe Incorporated Asymmetrische hairpin-target-capture-oligomere
US11098375B2 (en) 2012-02-24 2021-08-24 Gen-Probe Prodesse, Inc. Detection of Shiga toxin genes in bacteria
JP6196611B2 (ja) * 2012-03-05 2017-09-13 日本碍子株式会社 標的核酸の検出方法
AU2013205110B2 (en) 2012-04-24 2016-10-13 Gen-Probe Incorporated Compositions, Methods and Kits to Detect Herpes Simplex Virus Nucleic Acids
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
CN104755630B (zh) 2012-08-30 2019-01-11 简·探针公司 多阶段核酸扩增
AU2013205122B2 (en) 2012-10-11 2016-11-10 Gen-Probe Incorporated Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid
AU2013205090B2 (en) 2012-12-07 2016-07-28 Gen-Probe Incorporated Compositions and Methods for Detecting Gastrointestinal Pathogen Nucleic Acid
JP6441893B2 (ja) * 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
WO2015023892A1 (en) 2013-08-14 2015-02-19 Gen-Probe Incorporated Compositions and methods for detecting hev nucleic acid
AU2014384731B2 (en) 2014-02-28 2017-12-14 Gen-Probe Incorporated Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
GB2528344B (en) 2014-02-28 2018-10-31 Gen Probe Inc Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
GB2555995B (en) 2014-10-20 2019-06-26 Gen Probe Inc Red blood cell lysis solution
US10865433B2 (en) 2015-01-09 2020-12-15 Gen-Probe Incorporated Reaction mixtures for diagnosing bacterial vaginosis
US20160258938A1 (en) * 2015-03-04 2016-09-08 Headway Technologies, Inc. Method of detecting an analyte in a sample
WO2016149357A1 (en) 2015-03-16 2016-09-22 Gen-Probe Incorporated Methods and compositions for detecting bacterial nucleic acid and diagnosing bacterial vaginosis
EP3400309B1 (de) 2016-01-04 2025-03-19 Gen-Probe Incorporated Verfahren und zusammensetzungen zum nachweis von candida-spezies
US12104206B2 (en) 2016-02-11 2024-10-01 Htg Molecular Diagnostics, Inc. Method of direct target sequencing using nuclease protection
JP6584986B2 (ja) * 2016-03-18 2019-10-02 株式会社東芝 核酸検出方法
CA3021914C (en) 2016-04-27 2024-04-30 Gen-Probe Incorporated Blood cell lysis reagent
EP3469103B1 (de) 2016-06-10 2021-10-06 Gen-Probe Incorporated Zusammensetzungen und verfahren für den nachweis von zikavirus-nukleinsäure
CA3040907A1 (en) 2016-10-19 2018-04-26 Gen-Probe Incorporated Compositions and methods for detecting or quantifying hepatitis c virus
CN110088302B (zh) 2016-11-21 2025-02-25 简·探针公司 用于检测或定量乙型肝炎病毒的组合物和方法
AU2018237578B2 (en) 2017-03-24 2024-03-07 Gen-Probe Incorporated Compositions and methods for detecting or quantifying Parainfluenza virus
WO2018183124A1 (en) 2017-03-25 2018-10-04 Gen-Probe Incorporated Compositions, methods and kits to detect adenovirus, metapneumovirus and/or rhinovirus nucleic acids
CA3062075C (en) 2017-05-11 2024-04-02 Gen-Probe Incorporated Compositions and methods for isolating target nucleic acids
WO2018226798A1 (en) 2017-06-07 2018-12-13 Gen-Probe Incorporated Detecting babesia species nucleic acid in a sample
WO2018227182A2 (en) * 2017-06-09 2018-12-13 University Of Houston System Force-modulated hybridization for visualizing nucleic acid length and function
WO2019032809A1 (en) 2017-08-11 2019-02-14 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING STAPHYLOCOCCUS AUREUS
WO2019099629A1 (en) 2017-11-17 2019-05-23 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING C1orf43 NUCLEIC ACID
US11662281B2 (en) 2017-12-13 2023-05-30 Gen-Probe Incorporated Compositions and methods for biological sample processing
EP3724354A1 (de) 2017-12-15 2020-10-21 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von toxigenem clostridium difficile
EP3749781B1 (de) 2018-02-06 2024-10-02 Gen-Probe Incorporated Dunkelrote farbstoffsondenformulierungen
CN120099195A (zh) 2018-06-13 2025-06-06 简·探针公司 用于检测b群链球菌核酸的组合物和方法
EP3830302B1 (de) 2018-08-01 2022-10-05 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von nukleinsäuren des epstein-barr-virus
EP3841224A1 (de) 2018-08-21 2021-06-30 Gen-Probe Incorporated Zusammensetzungen und verfahren zur amplifikation, detektion oder quantifizierung von menschlichem cytomegalovirus
EP3841222A1 (de) 2018-08-24 2021-06-30 Gen-Probe Incorporated Verfahren und zusammensetzungen zum nachweis von bakterieller nukleinsäure und zur diagnose von bakterieller vaginose
CN109337956B (zh) * 2018-09-07 2022-08-19 上海思路迪生物医学科技有限公司 基于ngs技术富集多基因茎环探针设计方法及试剂盒
CN113195743A (zh) 2018-10-22 2021-07-30 简·探针公司 扩增,检测或定量人类多瘤病毒bk病毒的组合物和方法
TW202030333A (zh) 2018-12-20 2020-08-16 美商簡 探針公司 用於檢測瘧原蟲物種核酸之組成物及方法
AU2020210753A1 (en) 2019-01-25 2021-09-02 Gen-Probe Incorporated Detection of drug-resistant mycoplasma genitalium
EP4389916A3 (de) 2019-03-22 2024-10-16 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von gruppe-a-streptococcus
WO2020256378A1 (ko) * 2019-06-17 2020-12-24 강릉원주대학교산학협력단 핵산 검출 방법
EP3994284A1 (de) 2019-07-03 2022-05-11 Gen-Probe Incorporated Trichomonas vaginalis oligonukleotide zur verwendung bei der bestimmung der anwesenheit in einer probe
CN114728263A (zh) 2019-11-14 2022-07-08 简·探针公司 用于捕获靶核酸的组合物和方法
AU2021230341C1 (en) 2020-03-04 2026-03-26 Gen-Probe Incorporated Compositions and methods for detecting SARS-CoV-2 nucleic acid
WO2021224873A1 (en) 2020-05-07 2021-11-11 Grifols Diagnostic Solutions Inc. Methods and compositions for detecting sars-cov-2 nucleic acid
WO2022197634A1 (en) 2021-03-15 2022-09-22 Gen-Probe Incorporated Compositions and methods for biological sample processing
CA3218222A1 (en) 2021-05-14 2022-11-17 Gen-Probe Incorporated Compositions and methods for detecting human adenovirus nucleic acid
EP4363610B1 (de) 2021-07-01 2025-08-06 Gen-Probe Incorporated Enzymformulierungen und reaktionsmischungen zur nukleinsäureamplifikation
EP4377481A1 (de) 2021-07-27 2024-06-05 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von gastrointestinalen pathogenen
CN114250224B (zh) * 2021-12-16 2024-02-27 福建和瑞基因科技有限公司 一种用于提取或检测样本中小分子rna的核酸组合物及其试剂盒和方法
CN114107446A (zh) * 2021-12-16 2022-03-01 福建和瑞基因科技有限公司 一种核酸的检测试剂盒及其检测方法
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers
WO2024081776A1 (en) 2022-10-13 2024-04-18 Gen-Probe Incorporated Cellularity control for nucleic acid assays
US20240124931A1 (en) * 2022-10-15 2024-04-18 Renato Rozental Material and method for diagnosis of traumatic brain injury
AU2023408044A1 (en) 2022-12-19 2025-07-31 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
WO2024161179A1 (en) 2023-01-31 2024-08-08 Mobidiag Oy Compositions and methods for detecting stx nucleic acids
EP4680772A1 (de) 2023-03-16 2026-01-21 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von gastrointestinalen parasiten
EP4455304A1 (de) 2023-04-28 2024-10-30 Mobidiag Oy Kontrollen für nukleinsäureamplifikationsverfahren
EP4455303A1 (de) 2023-04-28 2024-10-30 Mobidiag Oy Kontrollen für nukleinsäureamplifikationsverfahren
EP4454758A1 (de) 2023-04-28 2024-10-30 Mobidiag Oy Kontrollen für nukleinsäureamplifikationsverfahren
WO2026050332A1 (en) 2024-08-29 2026-03-05 Gen-Probe Incorporated Methods and compositions for amplifying and/or detecting rotavirus nucleic acids

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4174384A (en) * 1975-06-30 1979-11-13 Syva Company Fluorescence quenching with immunological pairs in immunoassays
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
US6221581B1 (en) * 1984-04-27 2001-04-24 Enzo Diagnostics, Inc. Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US5268266A (en) * 1984-05-07 1993-12-07 Genetics Institute, Inc. Process and nucleic acid construct for producing reagent complexes useful in determining target nucleotide sequences
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4824776A (en) * 1985-07-25 1989-04-25 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
US4725536A (en) * 1985-09-19 1988-02-16 Genetics Institute, Inc. Reagent polynucleotide complex with multiple target binding regions, and kit and methods
US4752566A (en) * 1985-12-17 1988-06-21 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
CA1273552A (en) 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
US5714380A (en) * 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
US5283174A (en) * 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
ATE142272T1 (de) * 1989-01-19 1996-09-15 Behringwerke Ag Nukleinsäure-amplifikation unter verwendung eines einzelprimers
ATE118824T1 (de) * 1989-03-10 1995-03-15 Amoco Corp Immobilisierte oligonukleotidsonden und ihre verwendungen.
EP0481999B1 (de) * 1989-03-10 1997-07-23 VYSIS, Inc. Interferenzverhinderung mit affinitätseinfangschemas
US5589335A (en) * 1989-04-05 1996-12-31 Amoco Corporation Hybridization promotion reagents
DE69032847T2 (de) * 1989-06-30 1999-05-12 Chiron Corp., Emeryville, Calif. Hydrophobe Nukleinsäure-Sonde
US5439793A (en) * 1990-07-19 1995-08-08 Syntex (U.S.A.) Inc. Method for producing a polynucleotide having an intramolecularly base-paired structure
US5849481A (en) * 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US6576419B1 (en) * 1993-07-23 2003-06-10 University Of Utah Research Foundation Assay procedure using fluorogenic tracers
CA2105364A1 (en) * 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US6156501A (en) * 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5861273A (en) 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US5631148A (en) * 1994-04-22 1997-05-20 Chiron Corporation Ribozymes with product ejection by strand displacement
AU2604995A (en) * 1994-06-01 1995-12-21 Hybridon, Inc. Branched oligonucleotides as pathogen-inhibitory agents
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
US5770365A (en) * 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
US5914230A (en) * 1995-12-22 1999-06-22 Dade Behring Inc. Homogeneous amplification and detection of nucleic acids
WO1997039008A1 (en) * 1996-04-12 1997-10-23 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US20030054376A1 (en) * 1997-07-07 2003-03-20 Mullis Kary Banks Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus
ATE339514T1 (de) * 1996-07-16 2006-10-15 Gen Probe Inc Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
ES2271994T3 (es) * 1997-05-02 2007-04-16 Gen-Probe Incorporated Hibridacion en dos pasos y captura de un polinucleotido.
US6534273B2 (en) * 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
EP1025120B1 (de) * 1997-10-27 2010-08-18 Boston Probes, Inc. Sich auf "pna molecular beacons" beziehende verfahren, testsätze und zusammensetzungen
CA2333253C (en) 1998-07-02 2010-09-07 Gen-Probe Incorporated Molecular torches
US6399302B1 (en) * 1998-08-21 2002-06-04 University Of Virginia Patent Foundation Signal generating oligonucleotide-based biosensor
ATE253075T1 (de) * 1998-08-27 2003-11-15 Bayer Ag Oligomerreinigung durch selektion mittels zweier enden
EP1147204A1 (de) * 1999-01-28 2001-10-24 Medical College Of Georgia Research Institute, Inc. Zusammensetzung und verfahren zur in vivo und in vitro abschwächung der genexpression mittels dobbelsträngigem rna
AU5282300A (en) * 1999-05-24 2000-12-12 Public Health Research Institute Of The City Of New York, Inc., The High specificity hairpin antisense oligonucleotides
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
WO2001094625A2 (en) * 2000-06-06 2001-12-13 Tm Bioscience Corporation Capture moieties for nucleic acids and uses thereof
US6451588B1 (en) * 2000-06-30 2002-09-17 Pe Corporation (Ny) Multipartite high-affinity nucleic acid probes
US6596490B2 (en) * 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
EP1366191A2 (de) * 2000-12-11 2003-12-03 Alexion Pharmaceuticals, Inc. 'nested' haarnadelförmige oligonukleotide zur amplifizierung von nukleinsäure
USH2220H1 (en) 2001-08-10 2008-07-01 Snp Consortium Identification and mapping of single nucleotide polymorphisms in the human genome
US7371580B2 (en) * 2001-08-24 2008-05-13 Agilent Technologies, Inc. Use of unstructured nucleic acids in assaying nucleic acid molecules
US7214780B2 (en) * 2001-11-02 2007-05-08 Gen-Probe Incorporated Probes, compositions and kits for determining the presence of Mycoplasma pneuomoniae in a test sample
JPWO2003100095A1 (ja) * 2002-05-08 2005-09-22 アークレイ株式会社 標的核酸の検出法
US7851150B2 (en) * 2002-12-18 2010-12-14 Third Wave Technologies, Inc. Detection of small nucleic acids
EP1604172B1 (de) * 2003-03-07 2009-06-17 The University of Medicine and Dentistry of New Jersey Optisch dekodierbare mikroträger, arrays und verfahren
CA2542232A1 (en) * 2003-06-09 2005-01-20 Alnylam Pharmaceuticals, Inc. Method for treating neurodegenerative disease by inhibiting alpha-synuclein
US20050037362A1 (en) 2003-08-11 2005-02-17 Eppendorf Array Technologies, S.A. Detection and quantification of siRNA on microarrays
WO2006007567A2 (en) 2004-07-01 2006-01-19 Gen-Probe Incorporated Methods and compositions to detect nucleic acids in a biological sample
US20060292616A1 (en) * 2005-06-23 2006-12-28 U.S. Genomics, Inc. Single molecule miRNA-based disease diagnostic methods

Also Published As

Publication number Publication date
US20060068417A1 (en) 2006-03-30
AU2009238261B2 (en) 2011-11-17
CA2572472A1 (en) 2006-01-19
EP1778867A2 (de) 2007-05-02
DK1778867T3 (da) 2010-08-02
US8460869B2 (en) 2013-06-11
WO2006007567A2 (en) 2006-01-19
EP1778867B1 (de) 2010-04-28
AU2005262357A1 (en) 2006-01-19
US8034554B2 (en) 2011-10-11
JP2008504842A (ja) 2008-02-21
DE602005020943D1 (de) 2010-06-10
CA2572472C (en) 2013-08-13
JP5020818B2 (ja) 2012-09-05
AU2009238261A1 (en) 2009-12-03
AU2005262357B2 (en) 2009-10-29
US20120231551A1 (en) 2012-09-13
US8551766B2 (en) 2013-10-08
WO2006007567A3 (en) 2006-03-30
US20110250599A1 (en) 2011-10-13

Similar Documents

Publication Publication Date Title
ATE466107T1 (de) Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
WO2001092572A1 (en) Kit and method for determining hla type
FI3872187T3 (fi) Koostumuksia ja menetelmiä näytteiden identifioinnin parantamiseksi indeksoiduissa nukleiinihappokirjastoissa
KR20210038541A (ko) 핵산 검출 방법
EP2366803A3 (de) Zusammensetzungen und Verfahren zum Nacheis des West Nil Viruses.
CN101275165A (zh) 用于鉴定亚甲基四氢叶酸还原酶(mthfr)基因型的核酸引物组和探针
CA2525413A1 (en) Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
AU2023201805B2 (en) Blood cell lysis reagent
ATE485391T1 (de) Zusammensetzungen, methoden und testssysteme zur bestimmung der präsenz von cryptosporidium organismen in einer testprobe
GB0317343D0 (en) Polynucleotide sequencing
ATE522606T1 (de) Verfahren zum nachweis von apfelmosaikvirus, primersatz für den nachweis und kit für den nachweis
KR930004477A (ko) 미코박테리아 프라이머 및 탐침
CA2747479C (en) Methods and reagents for shortening incubation times in hybridization assays
CN103740824B (zh) 一种两核苷酸实时合成测序图谱鉴定微生物种群的方法
ATE492649T1 (de) Microarray-basiertes verfahren zur amplifikation und detektion von nukleinsäuren in einem kontinuierlichen prozess
ATE466105T1 (de) Verfahren, zusammensetzungen und kits zur anreicherung und zum nachweis von mikroorganismen
CA2549059A1 (en) Oligonucleotides for the detection of hepatitis b virus
WO2003072721A3 (en) Detection by sliding template amplification
ATE463583T1 (de) Verfahren zum nachweis von chlamydia trachomatis und kit dafür
WO2005033340A3 (en) Methods and kits for hybridizing multiple probe panels to nucleic acid samples
EP1323835A3 (de) Methode zur Bestimmung einer Biospezies in einer Testprobe und dafür verwendeter Testsatz
KR100785011B1 (ko) 쌍이온 화합물을 이용한 핵산 혼성화 특이성을 증가시키는방법
ATE307906T1 (de) Verfahren zum nachweis von gram-positiven bakterien
ATE513924T1 (de) Verfahren zum nachweis einer basenmutation
Singer et al. Artificial nucleic acid probes and their applications in clinical microbiology

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties