ATE466957T1 - Methoden und reagenzien zur erhaltung der rna in zellen und geweben - Google Patents

Methoden und reagenzien zur erhaltung der rna in zellen und geweben

Info

Publication number
ATE466957T1
ATE466957T1 AT06000216T AT06000216T ATE466957T1 AT E466957 T1 ATE466957 T1 AT E466957T1 AT 06000216 T AT06000216 T AT 06000216T AT 06000216 T AT06000216 T AT 06000216T AT E466957 T1 ATE466957 T1 AT E466957T1
Authority
AT
Austria
Prior art keywords
reagents
methods
tissues
cells
rna
Prior art date
Application number
AT06000216T
Other languages
English (en)
Inventor
Eric S Lader
Original Assignee
Ambion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc filed Critical Ambion Inc
Application granted granted Critical
Publication of ATE466957T1 publication Critical patent/ATE466957T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04Preserving or maintaining viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AT06000216T 1998-07-31 1999-07-30 Methoden und reagenzien zur erhaltung der rna in zellen und geweben ATE466957T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/127,435 US6204375B1 (en) 1998-07-31 1998-07-31 Methods and reagents for preserving RNA in cell and tissue samples

Publications (1)

Publication Number Publication Date
ATE466957T1 true ATE466957T1 (de) 2010-05-15

Family

ID=22430096

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06000216T ATE466957T1 (de) 1998-07-31 1999-07-30 Methoden und reagenzien zur erhaltung der rna in zellen und geweben
AT99940837T ATE315665T1 (de) 1998-07-31 1999-07-30 Methoden und reagenzien zur erhaltung der rna in zellen und geweben

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT99940837T ATE315665T1 (de) 1998-07-31 1999-07-30 Methoden und reagenzien zur erhaltung der rna in zellen und geweben

Country Status (10)

Country Link
US (6) US6204375B1 (de)
EP (2) EP1657313B1 (de)
JP (1) JP4554080B2 (de)
AT (2) ATE466957T1 (de)
AU (1) AU745943B2 (de)
CA (1) CA2298841C (de)
DE (2) DE69929445T2 (de)
DK (1) DK1019545T3 (de)
ES (1) ES2255293T3 (de)
WO (1) WO2000006780A1 (de)

Families Citing this family (178)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1005633B1 (de) 1997-08-20 2008-09-03 The University Of Miami Hochqualitatives durchlaufendes verfahren zur fixierung, dehydratisierung, entfettung und imprägnation von geweben
US6793890B2 (en) 1997-08-20 2004-09-21 The University Of Miami Rapid tissue processor
US20080064108A1 (en) * 1997-12-10 2008-03-13 Tony Baker Urine Preservation System
US7569342B2 (en) * 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
US6777210B1 (en) * 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
US20040115692A1 (en) * 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
DE10031236A1 (de) * 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
US20030228600A1 (en) * 2000-07-14 2003-12-11 Eppendorf 5 Prime, Inc. DNA isolation method and kit
EP1356103B1 (de) * 2000-09-12 2010-10-20 Gen-Probe Incorporated Zusammensetzungen, methoden und testssysteme zur bestimmung der präsenz von cryptosporidium organismen in einer testprobe
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
KR100946980B1 (ko) * 2000-12-01 2010-03-17 리스판스 지네틱스, 인크. 표피 성장 인자 수용체 및 her2-neu 유전자 발현 및 이들 수준과 생존율의 상관성을 측정하는 방법
US6602670B2 (en) 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US6518416B1 (en) 2000-12-01 2003-02-11 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6872818B2 (en) * 2001-01-25 2005-03-29 Qiagen Gmbh Ammonium sulfate for neutralization of inhibitory effects
US6956111B2 (en) * 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
WO2003087297A2 (en) * 2001-08-08 2003-10-23 North Carolina State University Infectious disease microarray
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
WO2003031064A1 (en) 2001-10-12 2003-04-17 Becton, Dickinson And Company Method and apparatus for transporting biological samples
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
WO2003031065A1 (en) * 2001-10-12 2003-04-17 Becton, Dickinson And Company Method and basket apparatus for transporting biological samples
JP4283112B2 (ja) * 2001-10-29 2009-06-24 アークレイ株式会社 試験装置
DE10153957A1 (de) * 2001-11-06 2003-05-22 Quiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
US8088602B1 (en) 2001-12-05 2012-01-03 Elizabeth Gay Frayne Manipulation of RNA stability and protein synthesis using thio-phosphate
WO2003064605A2 (en) 2002-01-28 2003-08-07 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
AU2003299485B2 (en) * 2002-05-10 2008-10-09 The University Of Miami Preservation of RNA and morphology in cells and tissues
US7138226B2 (en) * 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
DE10222494A1 (de) * 2002-05-15 2003-11-27 Europroteome Ag Verfahren zur parallelen Gewinnung von RNA und Proteinen aus einer Gewebeprobe
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
ES2784011T3 (es) 2002-10-16 2020-09-21 Streck Inc Procedimiento y dispositivo para recoger y preservar las células para su análisis
US20040146871A1 (en) * 2003-01-29 2004-07-29 Hoyt Peter R. Method and high throughput adaptation thereof for RNA, DNA or protein isolation from animal tissues
US7517697B2 (en) * 2003-02-05 2009-04-14 Applied Biosystems, Llc Compositions and methods for preserving RNA in biological samples
EP1623010A4 (de) * 2003-04-25 2007-12-26 Janssen Pharmaceutica Nv Konservierung von rna in einer biologischen probe
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US7488579B2 (en) * 2003-05-06 2009-02-10 Biochain Institute, Inc. Methods and compositions to extract DNA, RNA and protein simultaneously from biological samples
EP1475434A1 (de) * 2003-05-09 2004-11-10 Oncoscience AG Verfahren zur Lagerung von Tumorzellen
AU2004283291B2 (en) * 2003-10-24 2010-08-12 The University Of Miami Simplified tissue processing
EP1692255A4 (de) * 2003-11-12 2010-12-08 Univ Boston Isolierung von nukleinsäure aus mundepithelzellen
EP1694692B1 (de) * 2003-12-16 2010-04-28 QIAGEN North American Holdings, Inc. Formulierungen und verfahren zum denaturieren von proteinen
US20050160701A1 (en) * 2003-12-24 2005-07-28 Stevens Timothy A. Tissue preservation assembly and method of making and using the same
US20050208501A1 (en) * 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
WO2005093052A1 (en) * 2004-03-26 2005-10-06 Fuji Photo Film Co., Ltd. Method for selectively separating and purifying rna and method for separating and purifying nucleic acid
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
AU2005245338B2 (en) * 2004-04-08 2011-11-17 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
US7361465B2 (en) 2004-09-07 2008-04-22 Applera Corporation Methods and compositions for tailing and amplifying RNA
CA2586532C (en) 2004-11-05 2014-03-25 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
DE102005013261A1 (de) * 2005-03-22 2006-09-28 Adnagen Ag Reagenz und Verfahren zur Verhinderung der zeitabhängigen Expression in biologischen Zellen
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
EP2360278A1 (de) 2005-04-14 2011-08-24 Trustees Of Boston University Diagnose für Lungenerkrankungen mittels Klassenvorhersage
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
WO2007011902A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Methods for rna profiling
WO2007016935A1 (en) 2005-07-29 2007-02-15 Histogenex Nv Methods, reagents and instrumentation for preparing impregnated tissue samples suitable for histopathological and molecular studies
ATE488587T1 (de) 2005-12-06 2010-12-15 Ambion Inc Rückübertragungs-primer und verfahren zu deren entwurf
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
WO2007100986A2 (en) * 2006-02-24 2007-09-07 Rosetta Inpharmatics Llc Extraction and diagnostic fluid devices, systems and methods of use
US20070207483A1 (en) * 2006-03-02 2007-09-06 Bio-Rad Laboratories, Inc. BUFFERS FOR DETECTION OF mRNA SEPARATED IN A MICROFLUIDIC DEVICE
EP1999472A2 (de) 2006-03-09 2008-12-10 The Trustees Of Boston University Diagnose- und prognoseverfahren für lungenerkrankungen mittels genexpressionsprofilen aus nasenepithelzellen
US20080027353A1 (en) * 2006-07-31 2008-01-31 Kliman Harvey J Method of making and using a library of biological information
WO2008040126A1 (en) 2006-10-06 2008-04-10 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
US7748283B2 (en) * 2007-02-16 2010-07-06 Whatman, Inc. Controlled transfer biological sample collection devices and methods of using such devices
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
DE102007025276A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Aromatische Alkohole zur Behandlung einer biologischen Probe
DE102007025277A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Verfahren zur Stabilisierung einer biologischen Probe
DE102007025275A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Butendisäure oder deren Derivate zur Behandlung einer biologischen Probe
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
AU2008302076B2 (en) 2007-09-19 2015-06-11 The Trustees Of Boston University Identification of novel pathways for drug development for lung disease
WO2009066695A1 (ja) * 2007-11-20 2009-05-28 Olympus Corporation 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
EP2214824A4 (de) * 2007-11-28 2015-08-19 Smart Tube Inc Vorrichtungen, systeme und verfahren zur entnahme, stimulation, stabilisierung und analyse einer biologischen probe
DE102007063019A1 (de) * 2007-12-21 2009-07-02 Humboldt-Universität Zu Berlin Verfahren und Arbeitsbesteck zum Aufschluss von Zellen sowie zur Konservierung und Extraktion von Nukleinsäuren aus lebenden Zellen oder Geweben
US20090298172A1 (en) * 2008-05-28 2009-12-03 Steven Paul Wheeler Histological specimen treatment apparatus and method
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US20100081928A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological Facilitation systems and methods
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
WO2010071833A1 (en) 2008-12-19 2010-06-24 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
EP2202320A1 (de) 2008-12-24 2010-06-30 Agendia B.V. Verfahren und Mittel zur Typisierung einer Probe mit Kolorektalkrebszellen
US11634747B2 (en) 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
WO2010088364A2 (en) 2009-01-30 2010-08-05 L'air Liquide Societe Anonyme Pour L'etude Et L'exploitation Des Procedes Georges Claude Process for preserving biological materials for extended periods of time
EP3290530B1 (de) 2009-02-18 2020-09-02 Streck Inc. Konservierung von zellfreien nukleinsäuren
US8669057B2 (en) 2009-05-07 2014-03-11 Veracyte, Inc. Methods and compositions for diagnosis of thyroid conditions
US20110027862A1 (en) * 2009-06-29 2011-02-03 Life Technologies Corporation Sample stabilization
IN2012DN00866A (de) * 2009-07-16 2015-07-10 Gen Hospital Corp
US20110213336A1 (en) 2009-08-05 2011-09-01 Cucin Robert L Method of and apparatus for sampling, processing and collecting tissue and reinjecting the same into human patients
US8348929B2 (en) 2009-08-05 2013-01-08 Rocin Laboratories, Inc. Endoscopically-guided tissue aspiration system for safely removing fat tissue from a patient
US8465471B2 (en) 2009-08-05 2013-06-18 Rocin Laboratories, Inc. Endoscopically-guided electro-cauterizing power-assisted fat aspiration system for aspirating visceral fat tissue within the abdomen of a patient
WO2011031892A1 (en) 2009-09-09 2011-03-17 The General Hospital Corporation Use of microvesicles in analyzing kras mutations
WO2011031877A1 (en) 2009-09-09 2011-03-17 The General Hospital Corporation Use of microvesicles in analyzing nucleic acid profiles
KR20130006603A (ko) * 2010-02-12 2013-01-17 인터디지탈 패튼 홀딩스, 인크 셀-에지 사용자 성능을 향상시키고 하향링크 협력 컴포넌트 캐리어를 통해 무선 링크 실패 조건을 시그널링하는 방법 및 장치
EP2580348B1 (de) 2010-06-14 2018-04-25 Qiagen GmbH Verfahren zur bestimmung von zielzellen oder -gewebe zur extraktion von biomolekülen aus nicht-formalin-fixierten biologischen proben
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012031008A2 (en) 2010-08-31 2012-03-08 The General Hospital Corporation Cancer-related biological materials in microvesicles
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
EP2622099B1 (de) 2010-09-28 2017-11-08 Agendia N.V. Verfahren und mittel zur typisierung einer krebszellen enthaltenden probe auf der basis von onkogenen signaltransduktionspfaden
EP2638057B1 (de) 2010-11-10 2019-03-06 Exosome Diagnostics, Inc. Verfahren zur isolierung von nukleinsäure enthaltenden partikeln und extraktion von nukleinsäuren daraus
US10036070B2 (en) 2010-12-23 2018-07-31 Agendia N.V. Methods and means for molecular classification of colorectal cancers
WO2012151391A2 (en) 2011-05-04 2012-11-08 Streck, Inc. Inactivated virus compositions and methods of preparing such compositions
GB201107466D0 (en) * 2011-05-05 2011-06-15 Loktionov Alexandre Device and method for non-invasive collection of colorectal mucocellular layer and disease detection
EP3150702B1 (de) 2011-06-19 2021-05-19 DNA Genotek, Inc. Vorrichtungen, lösungen und verfahren zur probenentnahme
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
US9175351B2 (en) 2011-07-13 2015-11-03 Agendia N.V. Means and methods for molecular classification of breast cancer
US9689833B2 (en) 2011-10-11 2017-06-27 Life Safety Distribution Ag Auxiliary micro-electrodes for diagnostics of electrochemical gas sensors
US9377435B2 (en) 2011-10-11 2016-06-28 Honeywell International Inc. Auxiliary gas diffusion electrodes for diagnostics of electrochemical gas sensors
WO2013117967A1 (en) * 2012-02-10 2013-08-15 Reametrix Inc. Compositions and methods for sputum samples preparation, and kit
US8927214B2 (en) 2012-04-18 2015-01-06 Syngenta Participations Ag Methods and compositions for dual extraction of protein and nucleic acid
US9808798B2 (en) 2012-04-20 2017-11-07 California Institute Of Technology Fluidic devices for biospecimen preservation
JP6196661B2 (ja) 2012-04-20 2017-09-13 スリップチップ, エルエルシー サンプル調製または自律分析のための流体デバイスおよびシステム
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
CN102680295B (zh) * 2012-05-21 2016-01-20 江苏省中医院 一种从he染色片提取残微核酸的方法
US9383297B2 (en) 2012-07-03 2016-07-05 The University Of Akron Decalcification solution with preservation of RNA
US20150275306A1 (en) 2012-10-10 2015-10-01 Stichting Het Nederlands Kanker Instituut Antoni van Leeuwenhoek Ziekenhuis Methods and means for predicting resistance to anti-cancer treatment
WO2014080381A1 (en) 2012-11-26 2014-05-30 Ecole Polytechnique Federale De Lausanne (Epfl) Colorectal cancer classification with differential prognosis and personalized therapeutic responses
EP3249054A1 (de) 2012-12-20 2017-11-29 Biomatrica, INC. Formulierungen und verfahren zur stabilisierung von pcr-reagenzien
CA2897360C (en) * 2013-01-24 2017-01-03 F. Hoffmann-La Roche Ag Rt-qpcr analysis of micro-dissected material from stained ffpet section
WO2014186036A1 (en) 2013-03-14 2014-11-20 Allegro Diagnostics Corp. Methods for evaluating copd status
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
US10368541B2 (en) 2013-03-15 2019-08-06 Creatv Microtech, Inc. Urine preservative reagent for microfiltration
EP4136972A1 (de) 2013-07-24 2023-02-22 Streck, Inc. Zusammensetzungen und verfahren zur stabilisierung zirkulierender tumorzellen
WO2015080585A1 (en) 2013-11-28 2015-06-04 Stichting Het Nederlands Kanker Instituut-Antoni van Leeuwenhoek Ziekenhuis Methods for molecular classification of brca-like breast and/or ovarian cancer
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US12297505B2 (en) 2014-07-14 2025-05-13 Veracyte, Inc. Algorithms for disease diagnostics
US10954507B2 (en) * 2014-07-17 2021-03-23 Qiagen Gmbh Method for isolating RNA with high yield
CN114606309A (zh) 2014-11-05 2022-06-10 威拉赛特公司 使用机器学习和高维转录数据的诊断系统和方法
CN104480012A (zh) * 2014-11-27 2015-04-01 南方医科大学 一种粪便细菌的保存液
CN104673717B (zh) * 2015-02-12 2017-11-28 中国医科大学 一种生物组织rna保护试剂及其制备方法和应用
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
CN107406839B (zh) * 2015-03-06 2021-11-05 生物辐射实验室股份有限公司 稳定的rna溶液
US20180051346A1 (en) 2015-03-17 2018-02-22 Stichting Het Nederlands Kanker Instituut-Antoni van Leeuwenhoek Ziekenhuis Methods and means for subtyping invasive lobular breast cancer
ES2758084T3 (es) 2015-03-20 2020-05-04 Iq Products B V Marcador novedoso para diabetes gestacional
WO2016153355A2 (en) 2015-03-26 2016-09-29 Microbiome Limited Novel isolation and amplification process control
US10365189B2 (en) 2015-05-07 2019-07-30 Steven Wheeler Histological specimen treatment
EP3754011B1 (de) 2015-09-09 2022-02-16 Drawbridge Health, Inc. Vorrichtungen zur probeentnahme, stabilisierung und konservierung
JP2017099376A (ja) * 2015-11-19 2017-06-08 株式会社日吉 環境水試料の前処理方法、環境水試料の前処理剤、並びに、生物相又は生物の量の推定方法
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
KR102589056B1 (ko) 2015-12-08 2023-10-12 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
EP3430135B1 (de) 2016-03-19 2026-02-11 QIAGEN GmbH Stabilisierung von rna
KR20220139426A (ko) * 2016-03-31 2022-10-14 버클리 라잇츠, 인크. 핵산 안정화 시약, 키트들, 및 그 이용 방법들
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
EP4180531A3 (de) 2016-05-12 2023-08-23 Trustees of Boston University Genexpressionssignatur des nasenepithels und klassifikator zur vorhersage von lungenkrebs
WO2017198715A1 (en) * 2016-05-17 2017-11-23 R-Biopharm Ag Nucleic acid stabilization agent
US20190153427A1 (en) * 2016-07-08 2019-05-23 President And Fellows Of Harvard College Determination of rna in blood or other fluids
WO2018009915A1 (en) 2016-07-08 2018-01-11 Trustees Of Boston University Gene expression-based biomarker for the detection and monitoring of bronchial premalignant lesions
WO2018022991A1 (en) 2016-07-29 2018-02-01 Streck, Inc. Suspension composition for hematology analysis control
WO2018128544A1 (en) 2017-01-06 2018-07-12 Agendia N.V. Biomarkers for selecting patient groups, and uses thereof.
HUE062204T2 (hu) 2017-01-16 2023-10-28 Spectrum Solutions L L C Nukleinsavkonzerváló oldat és eljárások alkalmazására
EP3571314B1 (de) * 2017-01-22 2023-12-20 Chryos, LLC Zusammensetzung und verfahren zur verwendung davon zur konservierung von zellen zur analyse
US20190360051A1 (en) 2017-02-17 2019-11-28 Stichting Vumc Swarm intelligence-enhanced diagnosis and therapy selection for cancer using tumor- educated platelets
US11168323B2 (en) 2017-06-01 2021-11-09 Nantomics Llc DNA stabilization of RNA
CN107475251B (zh) * 2017-09-19 2021-02-02 成都导胜生物技术有限公司 一种裂解液及其在保存组织或细胞、提取rna中的应用
US12378543B2 (en) 2017-10-19 2025-08-05 Streck Llc Compositions for hemolysis and coagulation regulation and stabilization of extracellular vesicles
CN108094407A (zh) * 2017-12-29 2018-06-01 浙江今复康生物科技有限公司 一种细胞保存液及痰液细胞参考品的制备方法
US10046322B1 (en) 2018-03-22 2018-08-14 Talis Biomedical Corporation Reaction well for assay device
WO2020106891A1 (en) 2018-11-20 2020-05-28 Spectrum Solutions, Llc Sample collection system including sealing cap and valve
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
EP3983540A1 (de) * 2019-06-16 2022-04-20 Yeda Research and Development Co. Ltd Verfahren zur stabilisierung intrazellulärer rna
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system
WO2021118349A1 (en) 2019-12-10 2021-06-17 Prinses Máxima Centrum Voor Kinderoncologie B.V. Methods of typing germ cell tumors
US12485166B2 (en) 2020-02-06 2025-12-02 Longhorn Vaccines And Diagnostics, Llc Vaccines for the treatment and prevention of zoonotic infections
CN111363729B (zh) * 2020-03-12 2021-04-16 苏州白垩纪生物科技有限公司 一种rna病毒灭活保存液及其应用
CN111088319B (zh) * 2020-03-25 2020-07-21 深圳市上为生物科技有限公司 一种灭活型病毒样本rna保存液及其制备方法
WO2021221500A1 (en) 2020-04-27 2021-11-04 Agendia N.V. Treatment of her2 negative, mammaprint high risk 2 breast cancer.
IT202000018682A1 (it) * 2020-07-30 2022-01-30 Biofarma S R L Soluzione acquosa per la conservazione di acidi nucleici
WO2022087122A1 (en) 2020-10-20 2022-04-28 Longhorn Vaccines And Diagnostics, Llc Immunogenic antigens
WO2022169594A1 (en) * 2021-02-04 2022-08-11 Longhorn Vaccines And Diagnostics, Llc Multipurpose compositions for collecting and transporting biological material
WO2023019124A1 (en) * 2021-08-09 2023-02-16 University Of Central Florida Research Foundation, Inc. Method to preserve nucleic acids in water samples
EP4433031A1 (de) 2021-11-19 2024-09-25 Branca Bunus Limited Zusammensetzung mit einem in einem arzneimittelabgabevehikel verpackten therapeutischen wirkstoff
CN114410744A (zh) * 2022-01-27 2022-04-29 深圳安吉康尔医学检验实验室 一种处理样本的方法及其核酸提取方法与文库
WO2023162065A1 (ja) * 2022-02-24 2023-08-31 Blue Industries株式会社 生体試料保存剤、生体試料保存剤の製造方法、生体試料保存方法及び生体試料保存のためのキット
CN116103280B (zh) * 2022-12-28 2024-03-15 上海亿康医学检验所有限公司 样本保存液及其在染色体非整倍体筛查和/或转录组检测中的应用
CN120948678B (zh) * 2025-10-17 2026-02-03 苏州药明康德新药开发有限公司 一种适用于质谱和荧光检测的rna药物稳定剂及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2623276A1 (de) * 1976-05-24 1977-12-08 Max Planck Gesellschaft Verfahren zur fraktionierung und trennung von proteinen
US4746539A (en) * 1983-11-23 1988-05-24 The Ohio State University Research Foundation Purification of cancer-associated protein and preparation of antibody thereto
JPH0684543B2 (ja) 1985-12-18 1994-10-26 呉羽化学工業株式会社 選択的化学メッキ法
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5010183A (en) 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
US5256571A (en) 1991-05-01 1993-10-26 Cytyc Corporation Cell preservative solution
US5422241A (en) * 1991-07-03 1995-06-06 Ambion, Inc. Methods for the recovery of nucleic acids from reaction mixtures
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
AU6230594A (en) 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
JP3163224B2 (ja) * 1994-10-14 2001-05-08 三菱レイヨン株式会社 菌体または固定化菌体の懸濁液の保存方法
US5938621A (en) * 1997-09-12 1999-08-17 Becton Dickinson And Company Collection container assembly
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples

Also Published As

Publication number Publication date
EP1657313A2 (de) 2006-05-17
US20120270316A1 (en) 2012-10-25
US8178296B2 (en) 2012-05-15
JP4554080B2 (ja) 2010-09-29
AU5461699A (en) 2000-02-21
EP1657313B1 (de) 2010-05-05
ATE315665T1 (de) 2006-02-15
EP1019545A1 (de) 2000-07-19
WO2000006780A1 (en) 2000-02-10
JP2002521071A (ja) 2002-07-16
US20030114651A1 (en) 2003-06-19
DK1019545T3 (da) 2006-05-29
DE69929445D1 (de) 2006-04-06
CA2298841C (en) 2012-01-24
US20100028852A1 (en) 2010-02-04
US20140295411A1 (en) 2014-10-02
DE69929445T2 (de) 2006-09-14
DE69942351D1 (de) 2010-06-17
CA2298841A1 (en) 2000-02-10
US6204375B1 (en) 2001-03-20
US20010016312A1 (en) 2001-08-23
ES2255293T3 (es) 2006-06-16
AU745943B2 (en) 2002-04-11
EP1019545B1 (de) 2006-01-11
US6528641B2 (en) 2003-03-04
EP1657313A3 (de) 2007-06-27

Similar Documents

Publication Publication Date Title
ATE315665T1 (de) Methoden und reagenzien zur erhaltung der rna in zellen und geweben
US8193001B2 (en) Compositions and methods for preserving RNA in biological samples
ATE266723T1 (de) Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen
NO20022945D0 (no) Fremgangsmåte for isolering av RNA fra formalin-fikserte paraffin-innstöpte vevspröver
EP1003835A4 (de) Kassette und system zur kryokonservierung
DE69815703D1 (de) Verbindungen mit leicht spaltbaren bindungen zur inaktivierung von pathogenen
KR20070115874A (ko) Dna 분석용 생물 시료를 보관 및 처리하는 시약 및 방법
US8394624B2 (en) Process for preserving biological materials for extended periods of time
WO2004061118A3 (en) Compositions and methods for nucleic acid extraction from biological samples
Arcarons et al. Cryoprotectant role of exopolysaccharide of Pseudomonas sp. ID1 in the vitrification of IVM cow oocytes
CN107447007B (zh) 一种dna类组织样品保存液及应用
WO2001042283A3 (en) Sex-chromosome-specific proteins, species specific and sperm specific proteins and methods for their identification and isolation
CA2484216A1 (en) Preservation of rna and morphology in cells and tissues
US20090023209A1 (en) Solution for the indefinite maintenance of nucleic acids in the cell of origin thereof
Muer et al. Cryogenic changes in seminal protein of cattle and buffalo
WO2004110372A3 (en) Room temperature storage of organs
WO2004050896A8 (en) A method and therapeutic platelets
CN116287120B (zh) 一种可常温保存且可60℃高温下保存唾液样本中人源细胞核酸dna与rna的保存液及其制备方法
WO2006033022A3 (en) Repair and protection factor scoring method for bioactive agents
WO1999016457A3 (en) Novel composition for treating, preventing and/or delaying ischemic cell death
Dore et al. Polar resin embedding for Macrotrachela quadricornifera
Luo et al. Active nuclear transport of chicken lipovitellin-2
Li Understanding Freshwater Mussel Adhesion Through Recombinant Synthesis of Dresseina Bugensis Foot Protein 7
Sulistiyani et al. Protein Characterization of Javan Cobra (Naja sputatrix) Venom Following Sun Exposure and Photo-Oxidation Treatment
Shaw et al. Fertilization and early embryology: Evaluation of propanediol, ethylene glycol, sucrose and antifreeze proteins on the survival of slow-cooled mouse pronuclear and 4-cell embryos

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties