ATE471373T1 - Expression von endogenen genen durch homologe rekombination eines vektorkonstruktes mit zellulärer dna - Google Patents

Expression von endogenen genen durch homologe rekombination eines vektorkonstruktes mit zellulärer dna

Info

Publication number
ATE471373T1
ATE471373T1 AT98949508T AT98949508T ATE471373T1 AT E471373 T1 ATE471373 T1 AT E471373T1 AT 98949508 T AT98949508 T AT 98949508T AT 98949508 T AT98949508 T AT 98949508T AT E471373 T1 ATE471373 T1 AT E471373T1
Authority
AT
Austria
Prior art keywords
expression
genes
gene
methods
nucleic acid
Prior art date
Application number
AT98949508T
Other languages
English (en)
Inventor
John Harrington
Original Assignee
Abt Holding Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abt Holding Co filed Critical Abt Holding Co
Application granted granted Critical
Publication of ATE471373T1 publication Critical patent/ATE471373T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT98949508T 1997-09-26 1998-09-25 Expression von endogenen genen durch homologe rekombination eines vektorkonstruktes mit zellulärer dna ATE471373T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94122397A 1997-09-26 1997-09-26
US15964398A 1998-09-24 1998-09-24
PCT/US1998/020094 WO1999015650A1 (en) 1997-09-26 1998-09-25 Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna

Publications (1)

Publication Number Publication Date
ATE471373T1 true ATE471373T1 (de) 2010-07-15

Family

ID=26856150

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98949508T ATE471373T1 (de) 1997-09-26 1998-09-25 Expression von endogenen genen durch homologe rekombination eines vektorkonstruktes mit zellulärer dna

Country Status (14)

Country Link
EP (1) EP1017803B1 (de)
JP (1) JP5377806B2 (de)
KR (1) KR20010024296A (de)
CN (1) CN100582230C (de)
AT (1) ATE471373T1 (de)
AU (1) AU750025B2 (de)
BR (1) BR9812540A (de)
CA (1) CA2304642C (de)
DE (1) DE69841723D1 (de)
DK (1) DK1017803T3 (de)
IL (2) IL135242A0 (de)
NO (1) NO20001497L (de)
NZ (1) NZ504185A (de)
WO (1) WO1999015650A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7316923B1 (en) 1997-09-26 2008-01-08 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
CN1384881A (zh) * 1999-02-19 2002-12-11 阿瑟西斯公司 非靶向活化内源基因的组合物和方法
CA2383642C (en) 1999-10-14 2014-03-25 Clontech Laboratories, Inc. Anthozoa derived chromophores/fluorophores and methods for using the same
WO2002000724A2 (en) 2000-06-28 2002-01-03 Amgen, Inc. Thymic stromal lymphopoietin receptor molecules and uses thereof
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
EP1352060A2 (de) * 2000-10-30 2003-10-15 Novozymes Biotech, Inc. Verfahren zur expression von endogenen genen mittels restriktion enzym-vermittelter integrierung
AU2002343616A1 (en) 2001-11-02 2003-05-19 Athersys, Inc. Methods for mutating genes in cells and animals using insertional mutagenesis
EP1456223B1 (de) 2001-12-19 2009-08-12 The University Of Chicago Schnellreifende fluoreszierende proteine und verfahren zu deren anwendung
US7662924B2 (en) 2002-02-22 2010-02-16 The Board Of Trustees Of The University Of Illinois Beta chain-associated regulator of apoptosis
ES2338111T3 (es) 2002-08-21 2010-05-04 Revivicor, Inc. Animales porcinos que carecen de cualquier expresion de alfa 1,3 galactosiltransferasa funcional.
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US8021833B2 (en) 2003-02-12 2011-09-20 Functional Genetics, Inc. Method for reducing HIV viral budding by administering a VPS28-specfic antibody that disrupts Gag-TSG101-VPS28 binding interactions
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
DK1734814T3 (en) 2004-03-17 2015-04-07 Revivicor Inc Tissue DERIVED FROM ANIMALS IS MISSING ANY EXPRESSION OF FUNCTIONAL ALPHA-1,3 galactosyltransferase
EP1732944B1 (de) 2004-04-07 2012-09-05 The University of Chicago Monomere, rotfluoreszierende proteine
EP1774017B1 (de) 2004-07-26 2013-05-15 Pfenex Inc. Verfahren zur verbesserten proteinexpression durch strain-engineering
NZ554824A (en) 2004-10-22 2010-10-29 Revivicor Inc Porcines that lack endogenous antibody chains and express exogenous immunoglobulins
PA8660701A1 (es) 2005-02-04 2006-09-22 Pfizer Prod Inc Agonistas de pyy y sus usos
JP2008544746A (ja) 2005-05-12 2008-12-11 ザイモジェネティクス， インコーポレイテッド 免疫応答を調節するための組成物および方法
US20090186097A1 (en) 2005-08-09 2009-07-23 David Lee Ayares Transgenic Ungulates Expressing CTLA4-IG and Uses Thereof
WO2007053732A2 (en) 2005-11-01 2007-05-10 Mayo Foundation For Medical Education And Research Promoter polymorphisms of the blys gene and use in diagnostic methods
US8014957B2 (en) 2005-12-15 2011-09-06 Fred Hutchinson Cancer Research Center Genes associated with progression and response in chronic myeloid leukemia and uses thereof
KR101193722B1 (ko) 2006-07-24 2013-01-11 바이오렉시스 파마슈티칼 코포레이션 엑센딘 융합 단백질
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2009059305A2 (en) 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
US8003325B2 (en) 2007-11-30 2011-08-23 Mayo Foundation For Medical Education And Research Polymorphisms of the BLyS gene and use in diagnostic methods
US8883739B2 (en) 2010-01-19 2014-11-11 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for male reproductive disorders
CN102669057B (zh) * 2011-03-18 2014-11-26 上海南方模式生物科技发展有限公司 一种调控动物内源基因表达的遗传修饰方法
KR101948248B1 (ko) * 2011-11-18 2019-02-14 한국생명공학연구원 단백질 발현 미세 조절방법
US10052364B2 (en) 2013-03-15 2018-08-21 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for cognitive disorders
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
ES2974625T3 (es) * 2014-03-14 2024-06-28 Cibus Us Llc Procedimientos y composiciones para aumentar la eficacia de la modificación génica dirigida mediante reparación génica mediada por oligonucleótidos
EP3226889A4 (de) 2014-11-19 2018-11-21 The Trustees of Columbia University in the City of New York Osteocalcin zur behandlung von gebrechlichkeit im zusammenhang mit alterung
WO2019222359A1 (en) * 2018-05-15 2019-11-21 Duke University Systems and methods for genetic manipulation of akkermansia species

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
ATE174058T1 (de) 1989-11-06 1998-12-15 Cell Genesys Inc Herstellung von proteinen mittels homologer rekombination
US5204939A (en) * 1989-12-14 1993-04-20 Fujitsu Limited Rule base processing system and rule evaluation control method therein
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6270989B1 (en) * 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
US5561053A (en) * 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
CN1384881A (zh) * 1999-02-19 2002-12-11 阿瑟西斯公司 非靶向活化内源基因的组合物和方法

Also Published As

Publication number Publication date
NO20001497D0 (no) 2000-03-22
JP2003535564A (ja) 2003-12-02
DE69841723D1 (de) 2010-07-29
KR20010024296A (ko) 2001-03-26
NO20001497L (no) 2000-05-25
EP1017803A1 (de) 2000-07-12
IL135242A0 (en) 2001-05-20
AU750025B2 (en) 2002-07-11
BR9812540A (pt) 2001-12-26
AU9581698A (en) 1999-04-12
CA2304642C (en) 2014-11-18
CN100582230C (zh) 2010-01-20
JP5377806B2 (ja) 2013-12-25
EP1017803B1 (de) 2010-06-16
CA2304642A1 (en) 1999-04-01
NZ504185A (en) 2003-02-28
CN1280615A (zh) 2001-01-17
DK1017803T3 (da) 2010-08-23
HK1027375A1 (en) 2001-01-12
IL135242A (en) 2011-06-30
EP1017803A4 (de) 2002-01-09
WO1999015650A9 (en) 2000-09-08
WO1999015650A1 (en) 1999-04-01

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