ATE471387T1 - Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle - Google Patents

Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle

Info

Publication number
ATE471387T1
ATE471387T1 AT03779324T AT03779324T ATE471387T1 AT E471387 T1 ATE471387 T1 AT E471387T1 AT 03779324 T AT03779324 T AT 03779324T AT 03779324 T AT03779324 T AT 03779324T AT E471387 T1 ATE471387 T1 AT E471387T1
Authority
AT
Austria
Prior art keywords
ptm
reporter molecule
optimal
trans
ptms
Prior art date
Application number
AT03779324T
Other languages
English (en)
Inventor
Lloyd Mitchell
Madaiah Puttaraju
Mariano Garcia-Blanco
Edward Otto
Yanping Yang
Original Assignee
Virxsys Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Virxsys Corp filed Critical Virxsys Corp
Application granted granted Critical
Publication of ATE471387T1 publication Critical patent/ATE471387T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT03779324T 2002-10-23 2003-10-23 Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle ATE471387T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42049802P 2002-10-23 2002-10-23
PCT/US2003/034102 WO2004038380A2 (en) 2002-10-23 2003-10-23 Screening methods for identification of efficient pre-trans-splicing molecules

Publications (1)

Publication Number Publication Date
ATE471387T1 true ATE471387T1 (de) 2010-07-15

Family

ID=32176581

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03779324T ATE471387T1 (de) 2002-10-23 2003-10-23 Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle

Country Status (8)

Country Link
US (1) US20060154257A1 (de)
EP (1) EP1579004B1 (de)
JP (1) JP4654033B2 (de)
AT (1) ATE471387T1 (de)
AU (1) AU2003285009B2 (de)
CA (1) CA2503247A1 (de)
DE (1) DE60333032D1 (de)
WO (1) WO2004038380A2 (de)

Families Citing this family (19)

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US7968334B2 (en) * 2004-01-23 2011-06-28 Virxsys Corporation Expression of apoAI and variants thereof using spliceosome mediated RNA trans-splicing
WO2005070023A2 (en) * 2004-01-23 2005-08-04 Intronn, Inc. Expression of apoa-1 and variants thereof using spliceosome mediated rna trans-splicing
WO2005070948A1 (en) * 2004-01-23 2005-08-04 Intronn, Inc. Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated rna trans splicing
US20060094110A1 (en) * 2004-07-30 2006-05-04 Mcgarrity Gerard J Use of spliceosome mediated RNA trans-splicing for immunotherapy
US7871795B2 (en) * 2004-10-08 2011-01-18 Virxsys Corporation Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins
DE102006034964B3 (de) * 2006-07-28 2007-09-06 Semikron Elektronik Gmbh & Co. Kg Anordnung mit einem Leistungshalbleiterbauelement und mit einer Kontakteinrichtung
EP2326711A2 (de) * 2008-08-20 2011-06-01 VIRxSYS Corporation Zusammensetzungen und verfahren zur erzeugung pluripotenter stammzellen
DK2844762T3 (en) * 2012-05-04 2018-03-26 Univ California TESTING FOR ANTIBIOTIC SENSITIVITY USING PROBRIOSOMAL RNA PROBLEMS
EP2943591A4 (de) * 2013-01-14 2016-07-20 Cellecta Inc Verfahren und zusammensetzungen für profiling von einzelzellenexpression
JP6496727B2 (ja) * 2013-07-23 2019-04-03 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California ベータラクタム系抗生物質に対する感受性を迅速に判定するためのアムジノシリン
AU2016355343C1 (en) 2015-11-19 2024-10-03 Lloyd G. Mitchell Compositions and methods for correction of heritable ocular disease
WO2017171654A1 (en) 2016-04-01 2017-10-05 National University Of Singapore Trans-splicing rna (tsrna)
IT201700105372A1 (it) 2017-09-20 2019-03-20 Fondazione St Italiano Tecnologia Molecola di acido nucleico funzionale e relativo uso
KR20250150157A (ko) 2018-04-17 2025-10-17 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 트랜스-스플라이싱 분자
CN110819707B (zh) * 2019-11-24 2023-07-18 中南大学湘雅医院 一种针对多种来源细胞样本中内部核糖体结合位点元件的高通量鉴定方法
GB202102118D0 (en) * 2021-02-15 2021-03-31 Cambridge Entpr Ltd RNA Trans-Splicing molecule
KR20250011939A (ko) 2022-05-13 2025-01-22 아시디엔 테라퓨틱스, 인크. Abca4 트랜스-스플라이싱 분자
WO2026012439A1 (en) * 2024-07-11 2026-01-15 Sichuan Real & Best Biotech Co., Ltd. Rna trans-splicing molecules and systems
CN121344095B (zh) * 2025-12-16 2026-04-28 北京肿瘤医院(北京大学肿瘤医院) 一种细胞内mRNA传感器开关的DNA文库筛选方法及应用

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Also Published As

Publication number Publication date
DE60333032D1 (de) 2010-07-29
JP4654033B2 (ja) 2011-03-16
AU2003285009A1 (en) 2004-05-13
EP1579004B1 (de) 2010-06-16
JP2006506984A (ja) 2006-03-02
CA2503247A1 (en) 2004-05-06
WO2004038380A2 (en) 2004-05-06
US20060154257A1 (en) 2006-07-13
EP1579004A4 (de) 2006-06-28
WO2004038380A3 (en) 2005-04-21
AU2003285009B2 (en) 2010-10-07
EP1579004A2 (de) 2005-09-28

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