ATE471387T1 - Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle - Google Patents

Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle

Info

Publication number
ATE471387T1
ATE471387T1 AT03779324T AT03779324T ATE471387T1 AT E471387 T1 ATE471387 T1 AT E471387T1 AT 03779324 T AT03779324 T AT 03779324T AT 03779324 T AT03779324 T AT 03779324T AT E471387 T1 ATE471387 T1 AT E471387T1
Authority
AT
Austria
Prior art keywords
ptm
reporter molecule
optimal
trans
ptms
Prior art date
Application number
AT03779324T
Other languages
English (en)
Inventor
Lloyd Mitchell
Madaiah Puttaraju
Mariano Garcia-Blanco
Edward Otto
Yanping Yang
Original Assignee
Virxsys Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Virxsys Corp filed Critical Virxsys Corp
Application granted granted Critical
Publication of ATE471387T1 publication Critical patent/ATE471387T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT03779324T 2002-10-23 2003-10-23 Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle ATE471387T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42049802P 2002-10-23 2002-10-23
PCT/US2003/034102 WO2004038380A2 (en) 2002-10-23 2003-10-23 Screening methods for identification of efficient pre-trans-splicing molecules

Publications (1)

Publication Number Publication Date
ATE471387T1 true ATE471387T1 (de) 2010-07-15

Family

ID=32176581

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03779324T ATE471387T1 (de) 2002-10-23 2003-10-23 Screening-verfahren zur identifizierung wirksamer prä-trans-spleissmoleküle

Country Status (8)

Country Link
US (1) US20060154257A1 (de)
EP (1) EP1579004B1 (de)
JP (1) JP4654033B2 (de)
AT (1) ATE471387T1 (de)
AU (1) AU2003285009B2 (de)
CA (1) CA2503247A1 (de)
DE (1) DE60333032D1 (de)
WO (1) WO2004038380A2 (de)

Families Citing this family (19)

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Publication number Priority date Publication date Assignee Title
US8053232B2 (en) * 2004-01-23 2011-11-08 Virxsys Corporation Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated RNA trans splicing
EP1716165A4 (de) * 2004-01-23 2008-06-18 Virxsys Corp Expression von apoa-1 und varianten davon unter verwendung des spleissosom-vermittelten rna-trans-spleissens
US7968334B2 (en) * 2004-01-23 2011-06-28 Virxsys Corporation Expression of apoAI and variants thereof using spliceosome mediated RNA trans-splicing
US20060094110A1 (en) * 2004-07-30 2006-05-04 Mcgarrity Gerard J Use of spliceosome mediated RNA trans-splicing for immunotherapy
US7871795B2 (en) * 2004-10-08 2011-01-18 Virxsys Corporation Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins
DE102006034964B3 (de) * 2006-07-28 2007-09-06 Semikron Elektronik Gmbh & Co. Kg Anordnung mit einem Leistungshalbleiterbauelement und mit einer Kontakteinrichtung
WO2010022194A2 (en) * 2008-08-20 2010-02-25 Virxsys Corporation Compositions and methods for generation of pluripotent stem cells
WO2013166460A1 (en) * 2012-05-04 2013-11-07 The Regents Of The University Of California Antibiotic susceptibility testing using probes for preribosomal rna
WO2014110509A1 (en) * 2013-01-14 2014-07-17 Cellecta, Inc. Methods and compositions for single cell expression profiling
MX2016000811A (es) * 2013-07-23 2016-10-13 Univ California Amdinocilina para la rápida determinación de la susceptibilidad a antibióticos beta-lactámicos.
CA3005474A1 (en) 2015-11-19 2017-05-26 The Trustees Of The University Of Pennsylvania Compositions and methods for correction of heritable ocular disease
EP3436590A4 (de) 2016-04-01 2019-12-04 National University of Singapore Transspleissen von rna (tsrna)
IT201700105372A1 (it) * 2017-09-20 2019-03-20 Fondazione St Italiano Tecnologia Molecola di acido nucleico funzionale e relativo uso
KR102866133B1 (ko) 2018-04-17 2025-09-30 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 트랜스-스플라이싱 분자
CN110819707B (zh) * 2019-11-24 2023-07-18 中南大学湘雅医院 一种针对多种来源细胞样本中内部核糖体结合位点元件的高通量鉴定方法
GB202102118D0 (en) * 2021-02-15 2021-03-31 Cambridge Entpr Ltd RNA Trans-Splicing molecule
EP4522758A4 (de) 2022-05-13 2026-05-06 Ascidian Therapeutics Inc Abca4-transspleissmoleküle
WO2026012439A1 (en) * 2024-07-11 2026-01-15 Sichuan Real & Best Biotech Co., Ltd. Rna trans-splicing molecules and systems
CN121344095B (zh) * 2025-12-16 2026-04-28 北京肿瘤医院(北京大学肿瘤医院) 一种细胞内mRNA传感器开关的DNA文库筛选方法及应用

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US5747072A (en) * 1993-07-30 1998-05-05 University Of Michigan Adenoviral-mediated gene transfer to synovial cells in vivo
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Also Published As

Publication number Publication date
US20060154257A1 (en) 2006-07-13
CA2503247A1 (en) 2004-05-06
EP1579004A4 (de) 2006-06-28
WO2004038380A2 (en) 2004-05-06
EP1579004A2 (de) 2005-09-28
DE60333032D1 (de) 2010-07-29
EP1579004B1 (de) 2010-06-16
AU2003285009B2 (en) 2010-10-07
JP2006506984A (ja) 2006-03-02
JP4654033B2 (ja) 2011-03-16
WO2004038380A3 (en) 2005-04-21
AU2003285009A1 (en) 2004-05-13

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