ATE474933T1 - Verfahren zum nachweis von pathogenen organismen - Google Patents
Verfahren zum nachweis von pathogenen organismenInfo
- Publication number
- ATE474933T1 ATE474933T1 AT03782268T AT03782268T ATE474933T1 AT E474933 T1 ATE474933 T1 AT E474933T1 AT 03782268 T AT03782268 T AT 03782268T AT 03782268 T AT03782268 T AT 03782268T AT E474933 T1 ATE474933 T1 AT E474933T1
- Authority
- AT
- Austria
- Prior art keywords
- hybridization
- pathogens
- amplification
- indicative
- nucleic acid
- Prior art date
Links
- 244000052769 pathogen Species 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 2
- 238000009396 hybridization Methods 0.000 abstract 5
- 230000003321 amplification Effects 0.000 abstract 4
- 238000001514 detection method Methods 0.000 abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000012544 monitoring process Methods 0.000 abstract 2
- 230000001717 pathogenic effect Effects 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Electrotherapy Devices (AREA)
- Geophysics And Detection Of Objects (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02027272A EP1426447A1 (de) | 2002-12-06 | 2002-12-06 | Verfahren zur Detection des pathogenen gram positiven Bacterien, Bvw. Staphylococcus, Enterococcus and Streptococcus |
| EP03007458 | 2003-04-04 | ||
| PCT/EP2003/013530 WO2004053155A1 (en) | 2002-12-06 | 2003-12-02 | Multiplex assay detection of pathogenic organisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE474933T1 true ATE474933T1 (de) | 2010-08-15 |
Family
ID=32510124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03782268T ATE474933T1 (de) | 2002-12-06 | 2003-12-02 | Verfahren zum nachweis von pathogenen organismen |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20060099596A1 (de) |
| EP (2) | EP1570079B1 (de) |
| JP (2) | JP4377375B2 (de) |
| CN (1) | CN105349657A (de) |
| AT (1) | ATE474933T1 (de) |
| AU (2) | AU2003289927A1 (de) |
| CA (2) | CA2507240C (de) |
| DE (1) | DE60333480D1 (de) |
| WO (2) | WO2004053155A1 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0316232A (pt) | 2002-11-12 | 2005-10-04 | Becton Dickinson Co | Métodos para determinar o estado de sepsia, para prognosticar o começo de sepsia e para diagnosticar a sìndrome de resposta inflamatória sistêmica em um indivìduo |
| CA2505843A1 (en) | 2002-11-12 | 2004-05-27 | Becton, Dickinson And Company | Diagnosis of sepsis or sirs using biomarker profiles |
| US7718361B2 (en) | 2002-12-06 | 2010-05-18 | Roche Molecular Systems, Inc. | Quantitative test for bacterial pathogens |
| CA2525482C (en) * | 2003-04-04 | 2015-02-17 | F. Hoffmann-La Roche Ag | Improved system for multicolor real-time pcr comprising 3 to 6 pairs of forester resonance energy transfer (fret) probes |
| JP2008538007A (ja) | 2005-04-15 | 2008-10-02 | ベクトン，ディッキンソン アンド カンパニー | 敗血症の診断 |
| EP1977001A4 (de) * | 2006-01-20 | 2009-12-02 | Genein Co Ltd | Oligonukleotid für den nachweis von mit sepsis assoziierten bakterien, sowie mikro-arrays und ein verfahren zum nachweisen der bakterien unter verwendung des oligonukleotids |
| ES2426038T3 (es) * | 2006-02-21 | 2013-10-18 | National University Corporation University Of Toyama | Procedimiento rápido para identificar el microorganismo causante de una enfermedad infecciosa |
| US8535888B2 (en) * | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
| DE102007041864B4 (de) | 2007-09-04 | 2012-05-03 | Sirs-Lab Gmbh | Verfahren zum Nachweis von Bakterien und Pilzen |
| US8669113B2 (en) | 2008-04-03 | 2014-03-11 | Becton, Dickinson And Company | Advanced detection of sepsis |
| WO2011078439A1 (en) * | 2009-12-24 | 2011-06-30 | Seegene, Inc. | Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals |
| US20130324436A1 (en) * | 2010-11-30 | 2013-12-05 | Diagon Kft | Procedure for nucleic acid-based diagnostic determination of bacterial germ counts and kit for this purpose |
| CN105121656A (zh) * | 2012-11-15 | 2015-12-02 | 以色列分子检测有限公司 | Pcr反应混合物及使用其的方法 |
| KR101586678B1 (ko) * | 2013-11-19 | 2016-01-21 | 한국표준과학연구원 | 단일 염기 다형성을 갖는 미생물 16S rDNA 유전자를 이용한 정량 분석 방법 |
| CN107513494A (zh) * | 2017-08-17 | 2017-12-26 | 湖北伽诺美生物科技有限公司 | 一种核酸检测卡及其使用方法 |
| JP7296043B2 (ja) * | 2017-08-30 | 2023-06-22 | 東洋製罐グループホールディングス株式会社 | 微生物密度の推定方法、及び微生物密度推定用マイクロアレイ |
| CN113528684A (zh) * | 2021-08-04 | 2021-10-22 | 领航基因科技（杭州）有限公司 | 用于鉴别革兰氏阴性菌和革兰氏阳性菌的数字pcr检测试剂盒和应用 |
| CN120230874B (zh) * | 2025-05-28 | 2025-09-23 | 铭时医疗科技(宁波)有限公司 | 一种检测血流感染病原体的引物探针组合、试剂盒及其应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1180647A (en) | 1981-07-17 | 1985-01-08 | Cavit Akin | Light-emitting polynucleotide hybridization diagnostic method |
| ATE202801T1 (de) | 1983-01-10 | 2001-07-15 | Gen Probe Inc | Verfahren zum nachweis, identifizieren und quantifizieren von organismen und viren |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| EP0452596A1 (de) | 1990-04-18 | 1991-10-23 | N.V. Innogenetics S.A. | Hibridizierungssonden aus dem Abstandsbereich zwischen den 16S- und 23S-rRNA-Genen und Nachweis von nichtviralen Mikroorganismen |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5750409A (en) | 1991-11-18 | 1998-05-12 | Boehringer Mannheim Gmbh | Pentacyclic compounds and their use as absorption or fluorescent dyes |
| US6025132A (en) * | 1994-06-24 | 2000-02-15 | Innogenetics N.V. | Probes targeted to rRNA spacer regions, methods and kits for using said probes, for the detection of respiratory tract pathogens |
| US6001564A (en) * | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| WO1997007238A2 (de) | 1995-08-17 | 1997-02-27 | Eberhard-Karls-Universität Tübingen | Extraktion, amplifikation und sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material |
| US5849488A (en) * | 1996-02-27 | 1998-12-15 | Oulutech Ltd. | DNA-sequence-based diagnosis of mastitis from a milk sample |
| ES2253778T3 (es) | 1996-06-04 | 2006-06-01 | University Of Utah Research Foundation | Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia. |
| JP4540754B2 (ja) | 1996-06-04 | 2010-09-08 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | Ｐｃｒ中のハイブリダイゼーションのモニタリング |
| DE10027113A1 (de) | 1999-12-23 | 2001-09-27 | Andreas Hoeft | Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| WO2002014555A2 (en) | 2000-08-11 | 2002-02-21 | University Of Utah Research Foundation | Single-labeled oligonucleotide probes |
| DE10100493A1 (de) * | 2001-01-08 | 2002-08-01 | Biotecon Diagnostics Gmbh | Nachweis von pathogenen Bakterien |
| US6699670B2 (en) * | 2001-03-01 | 2004-03-02 | The Johns Hopkins University | Quantitative assay for the simultaneous detection and speciation of bacterial infections |
| GB2377017A (en) * | 2001-06-28 | 2002-12-31 | Animal Health Inst | Detection of foot and mouth disease virus |
-
2003
- 2003-12-02 JP JP2005502306A patent/JP4377375B2/ja not_active Expired - Lifetime
- 2003-12-02 AU AU2003289927A patent/AU2003289927A1/en not_active Abandoned
- 2003-12-02 CA CA2507240A patent/CA2507240C/en not_active Expired - Lifetime
- 2003-12-02 US US10/534,915 patent/US20060099596A1/en not_active Abandoned
- 2003-12-02 AT AT03782268T patent/ATE474933T1/de active
- 2003-12-02 CN CN201510837509.6A patent/CN105349657A/zh active Pending
- 2003-12-02 EP EP03782268A patent/EP1570079B1/de not_active Expired - Lifetime
- 2003-12-02 WO PCT/EP2003/013530 patent/WO2004053155A1/en not_active Ceased
- 2003-12-02 DE DE60333480T patent/DE60333480D1/de not_active Expired - Lifetime
- 2003-12-05 WO PCT/US2003/038783 patent/WO2004053457A2/en not_active Ceased
- 2003-12-05 CA CA002507933A patent/CA2507933C/en not_active Expired - Lifetime
- 2003-12-05 AU AU2003300825A patent/AU2003300825A1/en not_active Abandoned
- 2003-12-05 JP JP2005508480A patent/JP4377378B2/ja not_active Expired - Lifetime
- 2003-12-05 EP EP03812827A patent/EP1570086B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2507933A1 (en) | 2004-06-24 |
| AU2003300825A1 (en) | 2004-06-30 |
| WO2004053457A3 (en) | 2004-09-23 |
| CA2507240A1 (en) | 2004-06-24 |
| JP4377378B2 (ja) | 2009-12-02 |
| WO2004053457A2 (en) | 2004-06-24 |
| AU2003300825A8 (en) | 2004-06-30 |
| DE60333480D1 (de) | 2010-09-02 |
| WO2004053155A1 (en) | 2004-06-24 |
| CN105349657A (zh) | 2016-02-24 |
| EP1570086A2 (de) | 2005-09-07 |
| EP1570086B1 (de) | 2013-02-20 |
| JP2006508694A (ja) | 2006-03-16 |
| JP4377375B2 (ja) | 2009-12-02 |
| CA2507240C (en) | 2011-05-24 |
| CA2507933C (en) | 2009-11-10 |
| US20060099596A1 (en) | 2006-05-11 |
| EP1570079A1 (de) | 2005-09-07 |
| EP1570086A4 (de) | 2006-01-11 |
| EP1570079B1 (de) | 2010-07-21 |
| JP2006518189A (ja) | 2006-08-10 |
| AU2003289927A1 (en) | 2004-06-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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