ATE476505T1 - Verfahren zur isolierung von nukleinsäuren mit verwendung von ethylenglykol-multimeren - Google Patents
Verfahren zur isolierung von nukleinsäuren mit verwendung von ethylenglykol-multimerenInfo
- Publication number
- ATE476505T1 ATE476505T1 AT06709721T AT06709721T ATE476505T1 AT E476505 T1 ATE476505 T1 AT E476505T1 AT 06709721 T AT06709721 T AT 06709721T AT 06709721 T AT06709721 T AT 06709721T AT E476505 T1 ATE476505 T1 AT E476505T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- solid support
- sample
- bound
- nucleic acids
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 10
- 102000039446 nucleic acids Human genes 0.000 title abstract 10
- 108020004707 nucleic acids Proteins 0.000 title abstract 10
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 3
- 238000002955 isolation Methods 0.000 title 1
- 239000007787 solid Substances 0.000 abstract 8
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 abstract 1
- 239000005977 Ethylene Substances 0.000 abstract 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical group C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 abstract 1
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0502887A GB0502887D0 (en) | 2005-02-11 | 2005-02-11 | Method |
| GB0503339A GB0503339D0 (en) | 2005-02-17 | 2005-02-17 | Method |
| US65495805P | 2005-02-23 | 2005-02-23 | |
| PCT/GB2006/000484 WO2006085104A1 (en) | 2005-02-11 | 2006-02-13 | Method for isolating nucleic acids comprising the use of ethylene glycol multimers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE476505T1 true ATE476505T1 (de) | 2010-08-15 |
Family
ID=36101350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06709721T ATE476505T1 (de) | 2005-02-11 | 2006-02-13 | Verfahren zur isolierung von nukleinsäuren mit verwendung von ethylenglykol-multimeren |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US8569477B2 (de) |
| EP (1) | EP1859038B1 (de) |
| JP (1) | JP2008529516A (de) |
| AT (1) | ATE476505T1 (de) |
| AU (1) | AU2006212024A1 (de) |
| CA (1) | CA2597482A1 (de) |
| WO (1) | WO2006085104A1 (de) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1859038B1 (de) * | 2005-02-11 | 2010-08-04 | Invitrogen Dynal AS | Verfahren zur isolierung von nukleinsäuren mit verwendung von ethylenglykol-multimeren |
| FR2904833A1 (fr) * | 2006-08-11 | 2008-02-15 | Bioquanta Sarl | Procede de dosage d'acide nuclieque par fluorescence |
| EP2264184A1 (de) * | 2009-06-22 | 2010-12-22 | Qiagen GmbH | Verfahren zur Amplifikation von DNA unter Verwendung von Tetraethylenglykol, Kit-of-parts dafür und dessen Verwendung |
| TWI407994B (zh) * | 2009-10-22 | 2013-09-11 | Ind Tech Res Inst | 分離核酸的方法、試劑及套組 |
| US9186668B1 (en) | 2010-06-04 | 2015-11-17 | Sandia Corporation | Microfluidic devices, systems, and methods for quantifying particles using centrifugal force |
| US8945914B1 (en) | 2010-07-08 | 2015-02-03 | Sandia Corporation | Devices, systems, and methods for conducting sandwich assays using sedimentation |
| US9795961B1 (en) * | 2010-07-08 | 2017-10-24 | National Technology & Engineering Solutions Of Sandia, Llc | Devices, systems, and methods for detecting nucleic acids using sedimentation |
| US8962346B2 (en) * | 2010-07-08 | 2015-02-24 | Sandia Corporation | Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation |
| GB201020095D0 (en) | 2010-11-26 | 2011-01-12 | Invitrogen Dynal As | Nucleic acid preparation method |
| JP6333737B2 (ja) | 2012-02-09 | 2018-05-30 | ライフ テクノロジーズ コーポレーション | コンジュゲートされたポリマー粒子およびその製造方法 |
| US9062079B2 (en) | 2012-02-09 | 2015-06-23 | Life Technologies Corporation | Hydrophobic diacrylamide compound |
| CN104245745B (zh) | 2012-02-09 | 2017-03-29 | 生命技术公司 | 亲水性聚合物颗粒及其制备方法 |
| US9244065B1 (en) | 2012-03-16 | 2016-01-26 | Sandia Corporation | Systems, devices, and methods for agglutination assays using sedimentation |
| US9903001B1 (en) | 2012-07-19 | 2018-02-27 | National Technology & Engineering Solutions Of Sandia, Llc | Quantitative detection of pathogens in centrifugal microfluidic disks |
| EP2900819B1 (de) | 2012-09-28 | 2020-10-28 | Cepheid | Verfahren zur dns- und rns-extraktion aus in paraffin eingebetteten festen gewebeproben |
| US9304128B1 (en) | 2013-02-01 | 2016-04-05 | Sandia Corporation | Toxin activity assays, devices, methods and systems therefor |
| EP2976424B1 (de) | 2013-03-18 | 2018-10-03 | Qiagen GmbH | Stabilisierung von biologischen proben |
| US9803238B1 (en) | 2013-11-26 | 2017-10-31 | National Technology & Engineering Solutions Of Sandia, Llc | Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid |
| DE102014211221A1 (de) | 2014-06-12 | 2015-12-17 | Robert Bosch Gmbh | Verfahren zur Anreicherung und/oder Aufreinigung von Nukleinsäuren |
| US9702871B1 (en) | 2014-11-18 | 2017-07-11 | National Technology & Engineering Solutions Of Sandia, Llc | System and method for detecting components of a mixture including a valving scheme for competition assays |
| US10254298B1 (en) | 2015-03-25 | 2019-04-09 | National Technology & Engineering Solutions Of Sandia, Llc | Detection of metabolites for controlled substances |
| WO2017004556A1 (en) | 2015-07-02 | 2017-01-05 | Life Technologies Corporation | Polymer substrates formed from carboxy functional acrylamide |
| EP3317426B1 (de) | 2015-07-02 | 2020-01-15 | Life Technologies Corporation | Konjugation von funktionellen hydrophilen carboxylkügelchen |
| WO2017007774A1 (en) | 2015-07-06 | 2017-01-12 | Life Technologies Corporation | Substrates and methods useful in sequencing |
| EP3325647B1 (de) * | 2015-07-24 | 2020-02-19 | Cepheid | Zusammensetzung und verfahren fur dna und rna extraierung vom gewebe proben |
| US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
| US10406528B1 (en) | 2016-08-04 | 2019-09-10 | National Technology & Engineering Solutions Of Sandia, Llc | Non-contact temperature control system for microfluidic devices |
| US10981174B1 (en) | 2016-08-04 | 2021-04-20 | National Technology & Engineering Solutions Of Sandia, Llc | Protein and nucleic acid detection for microfluidic devices |
| US10786811B1 (en) | 2016-10-24 | 2020-09-29 | National Technology & Engineering Solutions Of Sandia, Llc | Detection of active and latent infections with microfluidic devices and systems thereof |
| EP3399034B1 (de) * | 2017-05-05 | 2022-12-28 | Siemens Healthcare Diagnostics Inc. | Vorrichtung zur extraktion von nukleinsäuren aus biologischen probenmaterialien mit lösungsmittelfreien reagenzien |
| US10677728B2 (en) | 2017-08-17 | 2020-06-09 | Elitechgroup B.V. | Duplex stabilizing fluorescence quenchers for nucleic acid probes |
| US11155713B2 (en) | 2018-05-29 | 2021-10-26 | Elitechgroup, Inc. | Carborhodamine compounds and methods of preparation thereof |
| GB202017751D0 (en) * | 2020-11-10 | 2020-12-23 | Life Tech As | Sample protection |
| CN114213492B (zh) * | 2021-09-23 | 2025-04-01 | 武汉奥科鼎盛生物科技有限公司 | 一种dna纯化试剂、其制备方法及dna纯化方法 |
| GB2620799A (en) | 2022-07-22 | 2024-01-24 | Life Tech As | Particles |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU530410B2 (en) | 1978-02-21 | 1983-07-14 | Sintef | Preparing aqueous emulsions |
| NO155316C (no) | 1982-04-23 | 1987-03-11 | Sintef | Fremgangsmaate for fremstilling av magnetiske polymerpartikler. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| JPH033232A (ja) | 1989-05-30 | 1991-01-09 | Fujitsu Ltd | 化学気相成長装置 |
| GB9003253D0 (en) | 1990-02-13 | 1990-04-11 | Amersham Int Plc | Precipitating polymers |
| DE4432654C2 (de) * | 1994-09-14 | 1998-03-26 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren aus natürlichen Quellen |
| EP0775150B1 (de) * | 1994-02-07 | 1999-04-28 | QIAGEN GmbH | Verfahren zur abreicherung oder entfernung von endotoxinen |
| US5990301A (en) * | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| GB9411572D0 (en) * | 1994-06-09 | 1994-08-03 | Amersham Int Plc | Magnetic bead precipitation method |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| JPH09303232A (ja) | 1996-05-09 | 1997-11-25 | Toyota Motor Corp | コモンレール式ディーゼルエンジンの噴射管接続構造 |
| US5707812A (en) | 1996-08-06 | 1998-01-13 | Vical Incorporated | Purification of plasmid DNA during column chromatography |
| WO1999058664A1 (en) | 1998-05-14 | 1999-11-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6463909B2 (en) | 2000-01-25 | 2002-10-15 | Usui Kokusai Sangyo Kaisha Limited | Common rail |
| JP3558008B2 (ja) | 2000-06-08 | 2004-08-25 | トヨタ自動車株式会社 | 燃料噴射装置 |
| EP1349951A4 (de) | 2001-01-09 | 2005-08-24 | Whitehead Biomedical Inst | Verfahren und reagenzien zur isolierung von nukleinsäuren |
| ATE414711T1 (de) * | 2001-06-28 | 2008-12-15 | Mountain View Pharmaceuticals | Polymerstabilisierte proteinasen |
| SE0202552D0 (sv) * | 2002-08-27 | 2002-08-27 | Amersham Biosciences Ab | Recovery of plasmids in an aqueous two-phase system |
| US20040185449A1 (en) * | 2003-03-20 | 2004-09-23 | Quinn John J. | Method for preparing assay samples |
| US20040197780A1 (en) * | 2003-04-02 | 2004-10-07 | Agencourt Bioscience Corporation | Method for isolating nucleic acids |
| EP1859038B1 (de) | 2005-02-11 | 2010-08-04 | Invitrogen Dynal AS | Verfahren zur isolierung von nukleinsäuren mit verwendung von ethylenglykol-multimeren |
-
2006
- 2006-02-13 EP EP06709721A patent/EP1859038B1/de not_active Expired - Lifetime
- 2006-02-13 WO PCT/GB2006/000484 patent/WO2006085104A1/en not_active Ceased
- 2006-02-13 US US11/815,967 patent/US8569477B2/en not_active Expired - Fee Related
- 2006-02-13 AT AT06709721T patent/ATE476505T1/de not_active IP Right Cessation
- 2006-02-13 CA CA002597482A patent/CA2597482A1/en not_active Abandoned
- 2006-02-13 AU AU2006212024A patent/AU2006212024A1/en not_active Abandoned
- 2006-02-13 JP JP2007554647A patent/JP2008529516A/ja active Pending
-
2013
- 2013-09-24 US US14/035,890 patent/US9464316B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1859038B1 (de) | 2010-08-04 |
| US20090069554A1 (en) | 2009-03-12 |
| JP2008529516A (ja) | 2008-08-07 |
| US9464316B2 (en) | 2016-10-11 |
| US8569477B2 (en) | 2013-10-29 |
| WO2006085104A1 (en) | 2006-08-17 |
| US20140094597A1 (en) | 2014-04-03 |
| CA2597482A1 (en) | 2006-08-17 |
| AU2006212024A1 (en) | 2006-08-17 |
| EP1859038A1 (de) | 2007-11-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |