ATE477844T1 - Mikrofluidnetz zur bilden einer tröpfchenschlange und verfahren - Google Patents

Mikrofluidnetz zur bilden einer tröpfchenschlange und verfahren

Info

Publication number
ATE477844T1
ATE477844T1 AT07705996T AT07705996T ATE477844T1 AT E477844 T1 ATE477844 T1 AT E477844T1 AT 07705996 T AT07705996 T AT 07705996T AT 07705996 T AT07705996 T AT 07705996T AT E477844 T1 ATE477844 T1 AT E477844T1
Authority
AT
Austria
Prior art keywords
flow
port
draft
droplets
phase
Prior art date
Application number
AT07705996T
Other languages
English (en)
Inventor
Mark Davies
Tara Dalton
Julie Garvey
Kieran Curran
Damian Curtin
Original Assignee
Stokes Bio Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stokes Bio Ltd filed Critical Stokes Bio Ltd
Application granted granted Critical
Publication of ATE477844T1 publication Critical patent/ATE477844T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/712—Feed mechanisms for feeding fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/7172—Feed mechanisms characterised by the means for feeding the components to the mixer using capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/7176—Feed mechanisms characterised by the means for feeding the components to the mixer using pumps
    • B01F35/717613—Piston pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/75—Discharge mechanisms
    • B01F35/754—Discharge mechanisms characterised by the means for discharging the components from the mixer
    • B01F35/7547—Discharge mechanisms characterised by the means for discharging the components from the mixer using valves, gates, orifices or openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00353—Pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00479—Means for mixing reactants or products in the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B01J2219/00587—High throughput processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
AT07705996T 2006-02-07 2007-02-07 Mikrofluidnetz zur bilden einer tröpfchenschlange und verfahren ATE477844T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US76567106P 2006-02-07 2006-02-07
US84768506P 2006-09-28 2006-09-28
PCT/IE2007/000014 WO2007091229A1 (en) 2006-02-07 2007-02-07 A microfluidic droplet queuing network

Publications (1)

Publication Number Publication Date
ATE477844T1 true ATE477844T1 (de) 2010-09-15

Family

ID=37963878

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07705996T ATE477844T1 (de) 2006-02-07 2007-02-07 Mikrofluidnetz zur bilden einer tröpfchenschlange und verfahren

Country Status (5)

Country Link
US (4) US7993911B2 (de)
EP (1) EP1981625B1 (de)
AT (1) ATE477844T1 (de)
DE (1) DE602007008527D1 (de)
WO (1) WO2007091229A1 (de)

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US8968659B2 (en) 2003-09-05 2015-03-03 Stokes Bio Limited Sample dispensing
WO2005023427A1 (en) 2003-09-05 2005-03-17 Stokes Bio Limited A microfluidic analysis system
US9597644B2 (en) 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
EP1981625B1 (de) 2006-02-07 2010-08-18 Stokes Bio Limited Mikrofluidnetz zur bilden einer tröpfchenschlange und verfahren
US8501497B2 (en) 2006-02-07 2013-08-06 Stokes Bio Limited Forming sample combinations using liquid bridge systems
US8735169B2 (en) 2006-02-07 2014-05-27 Stokes Bio Limited Methods for analyzing agricultural and environmental samples
US8298833B2 (en) 2006-02-07 2012-10-30 Stokes Bio Limited Liquid bridge and system
WO2008038258A1 (en) * 2006-09-28 2008-04-03 Stokes Bio Limited Microfluidic connector
US20100297748A1 (en) * 2006-09-28 2010-11-25 Stokes Bio Limited Integrated fluidic circuits
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
EP4512526A3 (de) 2008-09-23 2025-06-04 Bio-Rad Laboratories, Inc. Tropfenbasiertes assaysystem
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9132394B2 (en) * 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
EP2432597A2 (de) 2009-05-19 2012-03-28 Stokes Bio Limited Probenahmevorrichtung
US8429735B2 (en) 2010-01-26 2013-04-23 Frampton E. Ellis Method of using one or more secure private networks to actively configure the hardware of a computer or microchip
US12401619B2 (en) 2010-01-26 2025-08-26 Frampton E. Ellis Computer or microchip with a secure system bios having a separate private network connection to a separate private network
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
EP2394740B1 (de) * 2010-06-09 2013-01-02 KIST-Europe Forschungsgesellschaft mbH Vorrichtung zur Dosierung von Zellen, Verfahren zur Dosierung von Zellen sowie Verwendung der Vorrichtung
USD678547S1 (en) * 2010-06-30 2013-03-19 Metaboscreen Co., Ltd. Micro flow channel chip
US9873118B2 (en) * 2010-12-30 2018-01-23 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
EP2694213B1 (de) 2011-04-08 2020-05-06 Stokes Bio Limited Biologisches detektionssystem
CN103764285B (zh) 2011-04-08 2016-02-10 斯多克斯生物有限公司 用于对流体进行充电的系统和方法
US9821312B2 (en) * 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
US11110458B2 (en) 2013-02-01 2021-09-07 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9779189B2 (en) 2013-07-17 2017-10-03 Texas Tech University System System and method for simulation and design of discrete droplet microfluidic systems
US9845908B2 (en) * 2015-03-31 2017-12-19 Ap Couplings, Inc. Lightweight quick connector system
US11499183B2 (en) 2017-06-28 2022-11-15 Bio-Rad Laboratories, Inc. System and method for droplet detection
US11253852B2 (en) 2017-08-17 2022-02-22 Abbott Point Of Care Inc. Devices, systems, and methods for performing optical assays
US11067526B2 (en) 2017-08-17 2021-07-20 Abbott Point Of Care Inc. Devices, systems, and methods for performing optical and electrochemical assays
CN110869746B (zh) 2017-08-17 2023-08-11 雅培医护站股份有限公司 利用通用电路系统执行光学和电化学测定的技术
US11045805B2 (en) 2017-11-01 2021-06-29 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
WO2019209273A1 (en) 2018-04-24 2019-10-31 Hewlett-Packard Development Company, L.P. Microfluidic devices
WO2019209374A1 (en) * 2018-04-24 2019-10-31 Hewlett-Packard Development Company, L.P. Sequenced droplet ejection to deliver fluids
US11325380B2 (en) 2018-07-17 2022-05-10 Hewlett-Packard Development Company, L.P. Droplet ejectors to provide fluids to droplet ejectors
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CN113070108B (zh) * 2021-03-01 2022-05-06 清华大学 图案化水凝胶微粒制备方法及微流控装置
CN117123287B (zh) * 2023-09-07 2026-03-24 广东省科学院生物与医学工程研究所 一种高通量液滴发生器

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US7655470B2 (en) * 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
EP1981625B1 (de) * 2006-02-07 2010-08-18 Stokes Bio Limited Mikrofluidnetz zur bilden einer tröpfchenschlange und verfahren

Also Published As

Publication number Publication date
US20150352513A1 (en) 2015-12-10
US20100015606A1 (en) 2010-01-21
US7993911B2 (en) 2011-08-09
WO2007091229A1 (en) 2007-08-16
EP1981625A1 (de) 2008-10-22
DE602007008527D1 (de) 2010-09-30
US8563244B2 (en) 2013-10-22
US20140051604A1 (en) 2014-02-20
US9108177B2 (en) 2015-08-18
EP1981625B1 (de) 2010-08-18
US20120003658A1 (en) 2012-01-05

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