ATE479669T1 - Markierungsreagentien, markierte analyten, einschliesslich mischungen davon, und davon abgeleitete fragmentionen und verfahren zu deren analyse - Google Patents

Markierungsreagentien, markierte analyten, einschliesslich mischungen davon, und davon abgeleitete fragmentionen und verfahren zu deren analyse

Info

Publication number
ATE479669T1
ATE479669T1 AT05705033T AT05705033T ATE479669T1 AT E479669 T1 ATE479669 T1 AT E479669T1 AT 05705033 T AT05705033 T AT 05705033T AT 05705033 T AT05705033 T AT 05705033T AT E479669 T1 ATE479669 T1 AT E479669T1
Authority
AT
Austria
Prior art keywords
methods
labeling reagents
labeled analytes
including mixtures
analysis
Prior art date
Application number
AT05705033T
Other languages
English (en)
Inventor
Subhakar Dey
Darryl Pappin
Subhasish Purkayastha
Sasi Pillai
James Coull
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/751,387 external-priority patent/US7355045B2/en
Priority claimed from US10/751,353 external-priority patent/US20050147982A1/en
Priority claimed from US10/822,639 external-priority patent/US20050147985A1/en
Priority claimed from US10/852,730 external-priority patent/US20050148087A1/en
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE479669T1 publication Critical patent/ATE479669T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D207/46—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with hetero atoms directly attached to the ring nitrogen atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
    • C07D241/06—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having one or two double bonds between ring members or between ring members and non-ring members
    • C07D241/08—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having one or two double bonds between ring members or between ring members and non-ring members with oxygen atoms directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D253/00—Heterocyclic compounds containing six-membered rings having three nitrogen atoms as the only ring hetero atoms, not provided for by group C07D251/00
    • C07D253/08—Heterocyclic compounds containing six-membered rings having three nitrogen atoms as the only ring hetero atoms, not provided for by group C07D251/00 condensed with carbocyclic rings or ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
    • C07D295/02—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms containing only hydrogen and carbon atoms in addition to the ring hetero elements
    • C07D295/027—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms containing only hydrogen and carbon atoms in addition to the ring hetero elements containing only one hetero ring
    • C07D295/03—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms containing only hydrogen and carbon atoms in addition to the ring hetero elements containing only one hetero ring with the ring nitrogen atoms directly attached to acyclic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
    • C07D295/04—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms
    • C07D295/14—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D295/145—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with the ring nitrogen atoms and the carbon atoms with three bonds to hetero atoms attached to the same carbon chain, which is not interrupted by carbocyclic rings
    • C07D295/15—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with the ring nitrogen atoms and the carbon atoms with three bonds to hetero atoms attached to the same carbon chain, which is not interrupted by carbocyclic rings to an acyclic saturated chain

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT05705033T 2004-01-05 2005-01-05 Markierungsreagentien, markierte analyten, einschliesslich mischungen davon, und davon abgeleitete fragmentionen und verfahren zu deren analyse ATE479669T1 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US10/751,387 US7355045B2 (en) 2004-01-05 2004-01-05 Isotopically enriched N-substituted piperazine acetic acids and methods for the preparation thereof
US10/751,353 US20050147982A1 (en) 2004-01-05 2004-01-05 Mixtures of isobarically labeled analytes and fragments ions derived therefrom
US10/751,354 US20050148771A1 (en) 2004-01-05 2004-01-05 Active esters of N-substituted piperazine acetic acids, including isotopically enriched versions thereof
US10/751,388 US7307169B2 (en) 2004-01-05 2004-01-05 Isotopically enriched N-substituted piperazines and methods for the preparation thereof
US10/822,639 US20050147985A1 (en) 2004-01-05 2004-04-12 Mixtures of isobarically labeled analytes and fragments ions derived therefrom
US10/852,730 US20050148087A1 (en) 2004-01-05 2004-05-24 Isobarically labeled analytes and fragment ions derived therefrom
PCT/US2005/000223 WO2005068446A1 (en) 2004-01-05 2005-01-05 Labeling reagents, labeled analytes, including mixtures thereof, and fragment ions derived therefrom and methods for the analysis thereof

Publications (1)

Publication Number Publication Date
ATE479669T1 true ATE479669T1 (de) 2010-09-15

Family

ID=34799954

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05705033T ATE479669T1 (de) 2004-01-05 2005-01-05 Markierungsreagentien, markierte analyten, einschliesslich mischungen davon, und davon abgeleitete fragmentionen und verfahren zu deren analyse

Country Status (7)

Country Link
US (4) US7307169B2 (de)
EP (2) EP2251334B1 (de)
AT (1) ATE479669T1 (de)
AU (1) AU2005205522A1 (de)
CA (1) CA2552304C (de)
DE (1) DE602005023260D1 (de)
WO (1) WO2005068446A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040219685A1 (en) 2003-01-30 2004-11-04 Applera Corporation Methods and mixtures pertaining to analyte determination using electrophilic labeling reagents
EP1916527A1 (de) * 2003-11-26 2008-04-30 Applera Corporation Analyse von Massenspektraldaten in ruhigen Bereichen
CA2542941C (en) * 2003-11-26 2013-02-12 Applera Corporation Analysis of mass spectral data in the quiet zones and label selection therefor
US7307169B2 (en) 2004-01-05 2007-12-11 Applera Corporation Isotopically enriched N-substituted piperazines and methods for the preparation thereof
US20050148087A1 (en) 2004-01-05 2005-07-07 Applera Corporation Isobarically labeled analytes and fragment ions derived therefrom
US7868547B2 (en) 2004-03-01 2011-01-11 Dh Technologies Development Pte. Ltd. Determination of analyte characteristics based upon binding properties
US20070054345A1 (en) * 2004-05-19 2007-03-08 Hunter Christie L Expression quantification using mass spectrometry
WO2005114700A2 (en) * 2004-05-19 2005-12-01 Applera Corporation Expression quantification using mass spectrometry
US20080206737A1 (en) * 2004-05-19 2008-08-28 Hunter Christie L Expression quantification using mass spectrometry
WO2006017208A1 (en) * 2004-07-12 2006-02-16 Applera Corporation Mass tags for quantitative analyses
US20070048752A1 (en) * 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
US20070037286A1 (en) * 2005-02-09 2007-02-15 Subhasish Purkayastha Thyroxine-containing compound analysis methods
US20060183238A1 (en) 2005-02-09 2006-08-17 Applera Corporation Amine-containing compound analysis methods
US8975404B2 (en) 2006-01-24 2015-03-10 Dh Technologies Development Pte. Ltd. Labeling reagents for analyte determination and methods and compounds used in making the same
CN101454488A (zh) 2006-05-26 2009-06-10 阿普里拉股份有限公司 用于羟基化化合物的标签试剂和方法
US7906341B2 (en) 2006-06-30 2011-03-15 Dh Technologies Development Pte, Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
US8362242B2 (en) * 2006-06-30 2013-01-29 Dh Technologies Development Pte. Ltd. Analyte determination utilizing mass tagging reagents comprising a non-encoded detectable label
KR101081053B1 (ko) 2008-07-18 2011-11-07 포항공과대학교 산학협력단 가변질량 라벨링제와 이를 이용한 아미노산 서열 및 단백질정량 동시 분석방법
WO2010141075A1 (en) 2009-05-31 2010-12-09 Dh Technologies Development Pte. Ltd. Specific analysis of ketone and aldehyde analytes using reagent compounds labeling strategies, and mass spectrometry workflow
WO2011047192A2 (en) * 2009-10-14 2011-04-21 Dana-Farber Cancer Institute, Inc. Protected amine labels and use in detecting analytes
EP2512639A4 (de) * 2009-12-15 2013-05-08 Dh Technologies Dev Pte Ltd Analyse von aminosäuren und aminhaltigen verbindungen mit markierungsreagenzien und lc-ms-workflow
KR101229704B1 (ko) 2010-08-23 2013-02-05 포항공과대학교 산학협력단 동중체로 표지된 단백질 및 펩티드를 큰 질량의 ｙ－타입 이온을 이용하여 정량분석하는 방법
WO2012026743A2 (ko) 2010-08-23 2012-03-01 포항공과대학교 산학협력단 라벨링제 및 이를 이용한 아미노산 서열 및 단백질 다중 정량 동시 분석방법
US8492163B2 (en) 2011-01-31 2013-07-23 Dh Technologies Development Pte. Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
US20120329661A1 (en) * 2011-05-18 2012-12-27 Guo Zengkui Isotope Labeling-Assisted Quantification (iLAQ) of Biological Compounds
CN104603617B (zh) 2012-05-18 2017-04-12 Dh科技发展私人贸易有限公司 使用定点衍生化和lc/ms/ms工作流程分析一组脑腱黄瘤病生物
US9835629B2 (en) 2012-05-18 2017-12-05 Pierce Biotechnology, Inc. Methods and reagents for biomolecule labeling, enrichment and gentle elution
GB201521903D0 (en) * 2015-12-11 2016-01-27 Electrophoretics Ltd Isorbaric mass labels
CN113514531B (zh) * 2021-04-27 2022-10-25 清华大学 一种化合物的碎片离子预测方法及应用
WO2026053185A1 (en) * 2024-09-09 2026-03-12 Electrophoretics Limited Mass spectrometric analysis

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1932995A1 (de) * 1969-06-28 1971-01-07 Bosch Gmbh Robert Steuereinrichtung zum Steuern eines ersten Druckmittelstromes in Abhaengigkeit eines zweiten Druckmittelstromes
US3860581A (en) * 1972-12-27 1975-01-14 American Home Prod Preparation of 1,4-benzodiazepines
DE2851953A1 (de) * 1978-12-01 1980-06-19 Thomae Gmbh Dr K Neue spectinomycylamine, verfahren zu ihrer herstellung und diese enthaltenden arzneimittel
AU5940186A (en) 1985-07-05 1987-01-08 Institut Fur Pflanzenschutzforschung 2, 6 - dimethyl - morpholine quartenary compounds
US4761415A (en) 1986-08-28 1988-08-02 Smithkline Beckman Corporation Dopamine-β-hydroxylase inhibitors
JPH01125357A (ja) 1987-11-06 1989-05-17 Dainippon Pharmaceut Co Ltd トリペプチドの誘導体
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5252296A (en) * 1990-05-15 1993-10-12 Chiron Corporation Method and apparatus for biopolymer synthesis
EP1130399B1 (de) * 1992-05-29 2009-04-01 The Rockefeller University Verfahren zur Bestimmung der Folge von Peptiden unter Verwendung eines Massenspektrometers
GB9300883D0 (en) 1993-01-18 1993-03-10 Pfizer Ltd Antiparasitic agents
AU7247896A (en) 1995-09-29 1997-04-17 Scripps Research Institute, The Protein signature analysis
US6613508B1 (en) 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
ES2213823T3 (es) * 1996-04-08 2004-09-01 Glaxo Group Limited Codificacion y analisis cualitativo de bibliotecas combinatorias basadas en masa.
US5780232A (en) 1996-05-28 1998-07-14 Atom Sciences, Inc. DNA sequencing, mapping, and diagnostic processes using hybridization and stable isotope labels of DNA
US6329180B1 (en) * 1996-09-13 2001-12-11 Alex M. Garvin Genetic analysis using peptide tagged in-vitro synthesized proteins
DE69723096T2 (de) 1996-10-04 2004-05-06 Dako A/S Sonden zur detektion von mycobakterien
JP3884087B2 (ja) 1997-01-15 2007-02-21 イクスツィリオン ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 質量標識結合ハイブリダイゼーションプローブ
IL130949A (en) * 1997-01-23 2004-12-15 Xzillion Gmbh & Co Kg Method for characterising polypeptides
CA2297158C (en) 1997-07-22 2009-12-22 Rapigene Inc. Methods and compounds for analyzing nucleic acids by mass spectrometry
JP4296608B2 (ja) * 1997-08-27 2009-07-15 田辺三菱製薬株式会社 Ｐｐａｒのアゴニスト及びアンタゴニストのスクリーニング方法
CA2301957A1 (en) 1997-09-23 1999-04-01 Sarissa Inc. Antisense oligonucleotides against thymidylate synthase
GB9823646D0 (en) * 1997-12-19 1998-12-23 Brax Genomics Ltd Compounds for mass spectrometry
US6428956B1 (en) 1998-03-02 2002-08-06 Isis Pharmaceuticals, Inc. Mass spectrometric methods for biomolecular screening
US6670194B1 (en) 1998-08-25 2003-12-30 University Of Washington Rapid quantitative analysis of proteins or protein function in complex mixtures
US6270976B1 (en) * 1998-09-15 2001-08-07 Brax Group Limited Characterizing nucleic acid by mass spectrometry
US6319476B1 (en) 1999-03-02 2001-11-20 Perseptive Biosystems, Inc. Microfluidic connector
US6629040B1 (en) 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
US6403309B1 (en) 1999-03-19 2002-06-11 Valigen (Us), Inc. Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
GB0006141D0 (en) 2000-03-14 2000-05-03 Brax Group Ltd Mass labels
WO2001086296A2 (en) 2000-05-05 2001-11-15 Agilix Corporation Highly multiplexed reporter carrier systems
CA2419159A1 (en) 2000-08-11 2002-02-21 Agilix Corporation Ultra-sensitive detection systems
US7045296B2 (en) 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
GB0115581D0 (en) 2001-06-26 2001-08-15 Glaxo Group Ltd Method of mass spectometry
PT1425586E (pt) 2001-09-14 2007-12-31 Electrophoretics Ltd Marcadores de massa
WO2003040288A2 (de) 2001-11-09 2003-05-15 Bayer Healthcare Ag Isotopencodierte affinitätsmarker 3
AU2003224674A1 (en) 2002-03-11 2003-09-29 Hk Pharmaceuticals, Inc. Compounds and methods for analyzing the proteome
US7473535B2 (en) 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
US20040219685A1 (en) 2003-01-30 2004-11-04 Applera Corporation Methods and mixtures pertaining to analyte determination using electrophilic labeling reagents
GB0306756D0 (en) 2003-03-24 2003-04-30 Xzillion Gmbh & Co Kg Mass labels
US7355045B2 (en) * 2004-01-05 2008-04-08 Applera Corporation Isotopically enriched N-substituted piperazine acetic acids and methods for the preparation thereof
US7307169B2 (en) 2004-01-05 2007-12-11 Applera Corporation Isotopically enriched N-substituted piperazines and methods for the preparation thereof

Also Published As

Publication number Publication date
AU2005205522A1 (en) 2005-07-28
US20080114169A1 (en) 2008-05-15
US7932388B2 (en) 2011-04-26
WO2005068446A1 (en) 2005-07-28
EP1701945A1 (de) 2006-09-20
US7307169B2 (en) 2007-12-11
EP2251334A1 (de) 2010-11-17
CA2552304C (en) 2012-03-27
US20050148771A1 (en) 2005-07-07
US8569304B2 (en) 2013-10-29
EP1701945B1 (de) 2010-09-01
US20050148773A1 (en) 2005-07-07
CA2552304A1 (en) 2005-07-28
EP2251334B1 (de) 2013-08-14
DE602005023260D1 (de) 2010-10-14
US20090227791A1 (en) 2009-09-10

Similar Documents

Publication Publication Date Title
ATE479669T1 (de) Markierungsreagentien, markierte analyten, einschliesslich mischungen davon, und davon abgeleitete fragmentionen und verfahren zu deren analyse
DE602006003012D1 (de) Verfahren und Nukleinsäuren für die Analyse von zellulären Proliferationsstörungen
WO2009086509A3 (en) Reagents for biomolecular labeling, detection and quantification employing raman spectroscopy
DE60318642D1 (de) Multi-Test-Analyse von Echzeit-Nukleinsäureamplifikationen
WO2008105896A3 (en) Analyte determination utilizing mass tagging reagents comprising a non-encoded detectable label
WO2007014045A3 (en) Methods for rapid identification and quantitation of nucleic acid variants
PT2089343E (pt) Química click para a produção de moléculas repórter
ATE419388T1 (de) Nukleinsäureanreicherung
WO2008039769A3 (en) Methods and devices for analyzing small rna molecules
ATE515702T1 (de) Verfahren, mixturen, kits und zusammensetzungen bezüglich der analytbestimmung
DK2253718T3 (da) Fast overflade til biomolekylafgivelse og analyse med høj omsætning
ATE431856T1 (de) Verbesserte bisulfitkonversion von dna
ATE490466T1 (de) Als interne funktionskontrolle in tests zum nachweis von seltenen zellen verwendete markierte zellen
BR0311175A (pt) Processo e sistema de reagente com complexo de enzima-coenzima não regenerável
ATE525649T1 (de) Nachweis von proteinen mit veränderter konformation
DE602004021185D1 (de) Auf bio-barcodes beruhender nachweis von zielanalyten
TW200636075A (en) Detection of nucleic acid variation by cleavage-amplification method
TW200720441A (en) New labelling strategies for the sensitive detection of analytes
WO2008020823A3 (en) Cooperative reporter systems, components, and methods for analyte detection
ATE519116T1 (de) Verfahren zur normierung der konzentration von analyten in einer urinprobe
DE60325382D1 (de) Verfahren zur diagnose von gebärmutterhalskrebs
DE602005008146D1 (de) Methode für die Quantifizierung der Ausgangs-Konzentration von Nukleinsäuren ausgehend von real-time Amplifikationsdaten
DE602005025401D1 (de) Hes verfahren unter verwendung davon
ATE525462T1 (de) Proteomanalyseverfahren für phosphoryliertes protein
NO20033884L (no) Fremgangsmåte for påvisning av nukleinsyremolekyler

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties