ATE479770T1 - Mikrowellengestützte deglycosylierung von proteinen zur molekulargewichtsbestimmung durch massenspektrometrie - Google Patents

Mikrowellengestützte deglycosylierung von proteinen zur molekulargewichtsbestimmung durch massenspektrometrie

Info

Publication number
ATE479770T1
ATE479770T1 AT07761533T AT07761533T ATE479770T1 AT E479770 T1 ATE479770 T1 AT E479770T1 AT 07761533 T AT07761533 T AT 07761533T AT 07761533 T AT07761533 T AT 07761533T AT E479770 T1 ATE479770 T1 AT E479770T1
Authority
AT
Austria
Prior art keywords
deglycosylation
proteins
molecular weight
mass spectrometry
assisted
Prior art date
Application number
AT07761533T
Other languages
English (en)
Inventor
David P Arnott
Jennie Lill
Wendy N Sandoval
Richard L Vandlen
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE479770T1 publication Critical patent/ATE479770T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/924Hydrolases (3) acting on glycosyl compounds (3.2)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00Assays, e.g. immunoassays or enzyme assays, involving carbohydrates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT07761533T 2006-05-02 2007-04-27 Mikrowellengestützte deglycosylierung von proteinen zur molekulargewichtsbestimmung durch massenspektrometrie ATE479770T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79733406P 2006-05-02 2006-05-02
PCT/US2007/067714 WO2007130875A2 (en) 2006-05-02 2007-04-27 Microwave assisted deglycosylation of proteins for molecular weight determination by mass spectrometry

Publications (1)

Publication Number Publication Date
ATE479770T1 true ATE479770T1 (de) 2010-09-15

Family

ID=38648516

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07761533T ATE479770T1 (de) 2006-05-02 2007-04-27 Mikrowellengestützte deglycosylierung von proteinen zur molekulargewichtsbestimmung durch massenspektrometrie

Country Status (8)

Country Link
US (1) US8163518B2 (de)
EP (1) EP2013356B1 (de)
JP (1) JP5219998B2 (de)
AT (1) ATE479770T1 (de)
AU (1) AU2007248253B2 (de)
CA (1) CA2650281C (de)
DE (1) DE602007008872D1 (de)
WO (1) WO2007130875A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011521255A (ja) * 2008-07-23 2011-07-21 インダストリー−アカデミック コーオペレーション ファンデーション キョンサン ナショナル ユニバーシティ ウイルス診断方法及びシステム
PT3387898T (pt) * 2013-03-15 2020-06-17 Mayo Found Medical Education & Res Identificação e monitorização de imunoglobulinas monoclonais pela massa molecular
EP3126826B1 (de) 2014-04-04 2020-12-16 Mayo Foundation for Medical Education and Research Isotypisierung von immunglobulinen unter verwendung einer genauen molekülmasse
US10690676B2 (en) 2014-07-29 2020-06-23 Mayo Roundation for Medical Education and Research Quantifying monoclonal antibody therapeutics by LC-MS/MS
EP3289364B1 (de) * 2015-04-30 2019-09-18 DH Technologies Development Pte. Ltd. Identifizierung von glycosylierungsformen
WO2017053932A1 (en) 2015-09-24 2017-03-30 Mayo Foundation For Medical Education And Research Identification of immunoglobulin free light chains by mass spectrometry
AU2017325022B2 (en) 2016-09-07 2022-10-13 Mayo Foundation For Medical Education And Research Identification and monitoring of cleaved immunoglobulins by molecular mass
WO2019055634A1 (en) 2017-09-13 2019-03-21 Mayo Foundation For Medical Education And Research IDENTIFICATION AND MONITORING OF A MACROPHAGE APOPTOSIS INHIBITOR
US12153052B2 (en) 2017-09-13 2024-11-26 Mayo Foundation For Medical Education And Research Identification and monitoring of immunoglobulin J chains

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5350686A (en) * 1992-04-16 1994-09-27 Pioneer Hi-Bred International, Inc. Microwave acceleration of enzyme-catalyzed modification of macromolecules
AUPN694895A0 (en) * 1995-12-01 1996-01-04 Macquarie Research Limited Desalting and purification of oligosaccharides and their derivatives
GB9821198D0 (en) * 1998-09-30 1998-11-25 Danisco Enzyme
US6960562B2 (en) * 1999-04-23 2005-11-01 Rhode Island Hospital, A Lifespan Partner Tribonectin polypeptides and uses thereof
US20030059432A1 (en) * 2000-02-11 2003-03-27 Dillon Davin C. Lipophilin complexes for use in cancer diagnosis and therapy
WO2002022808A2 (en) * 2000-09-18 2002-03-21 Biogen, Inc. Cripto mutant and uses thereof
US6720165B2 (en) * 2001-06-14 2004-04-13 Zyomix, Inc. Methods for making antibody fragments and compositions resulting therefrom
EP1406089A1 (de) * 2002-10-02 2004-04-07 Taiwan Unison Biotechnology Co. Ltd. Eine Methode zur selektiven Färbung wasserlöslicher Proteine unter Verwendung von Reaktivfarbstoffen
CN1852765A (zh) * 2002-10-18 2006-10-25 普罗梅加公司 用于分离分子的组合物
EP1740954B1 (de) * 2004-04-07 2015-08-19 Genentech, Inc. Massenspektrometrie von antikörperkonjugaten

Also Published As

Publication number Publication date
WO2007130875A2 (en) 2007-11-15
AU2007248253A1 (en) 2007-11-15
WO2007130875A3 (en) 2008-01-17
JP2009535646A (ja) 2009-10-01
EP2013356A2 (de) 2009-01-14
US8163518B2 (en) 2012-04-24
EP2013356B1 (de) 2010-09-01
JP5219998B2 (ja) 2013-06-26
CA2650281C (en) 2012-09-11
AU2007248253B2 (en) 2010-12-23
CA2650281A1 (en) 2007-11-15
US20070259398A1 (en) 2007-11-08
DE602007008872D1 (de) 2010-10-14

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