ATE480556T1 - NEW FLUORESCENT PROTEINS - Google Patents
NEW FLUORESCENT PROTEINSInfo
- Publication number
- ATE480556T1 ATE480556T1 AT03715128T AT03715128T ATE480556T1 AT E480556 T1 ATE480556 T1 AT E480556T1 AT 03715128 T AT03715128 T AT 03715128T AT 03715128 T AT03715128 T AT 03715128T AT E480556 T1 ATE480556 T1 AT E480556T1
- Authority
- AT
- Austria
- Prior art keywords
- sub
- proteins
- fluorescent proteins
- new fluorescent
- engineered
- Prior art date
Links
- 102000034287 fluorescent proteins Human genes 0.000 title abstract 2
- 108091006047 fluorescent proteins Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 238000010188 recombinant method Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention is directed to novel spontaneously fluorescent proteins having a unique chromophore formed from the amino acid sequence FR<SUB>1</SUB>R<SUB>2</SUB>R<SUB>3</SUB>F where R<SUB>1 </SUB>is Q or S; R<SUB>2 </SUB>is Y, W, F or H; and R<SUB>3 </SUB>is G, A or S (SEQ ID NO:34). The invention also encompasses the expression of nucleic acids that encode the proteins of the invention in a wide variety of engineered host cells, and the isolation of engineered proteins. In other embodiments, the invention comprises methods of use, generally including tagging a molecule or cell with the proteins of the invention by either chemical means or recombinant techniques.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37059802P | 2002-04-05 | 2002-04-05 | |
| US38796802P | 2002-06-11 | 2002-06-11 | |
| PCT/GB2003/001508 WO2003087150A2 (en) | 2002-04-05 | 2003-04-04 | Novel fluorescent proteins containing fqygf in the chromophore |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE480556T1 true ATE480556T1 (en) | 2010-09-15 |
Family
ID=29254416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03715128T ATE480556T1 (en) | 2002-04-05 | 2003-04-04 | NEW FLUORESCENT PROTEINS |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7335488B2 (en) |
| EP (1) | EP1492810B1 (en) |
| AT (1) | ATE480556T1 (en) |
| AU (1) | AU2003219319A1 (en) |
| DE (1) | DE60334087D1 (en) |
| WO (1) | WO2003087150A2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120058893B (en) * | 2025-02-24 | 2026-03-20 | 清华大学 | A mutant of a fluorescent protein and its application |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5491084A (en) | 1993-09-10 | 1996-02-13 | The Trustees Of Columbia University In The City Of New York | Uses of green-fluorescent protein |
| DE69604298T2 (en) | 1995-09-22 | 2000-05-18 | Bioimage A/S, Soeborg | VARIANTS OF THE GREEN FLUORESCENCE PROTEIN, GFP |
| US6027881A (en) | 1996-05-08 | 2000-02-22 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence |
| US6124128A (en) * | 1996-08-16 | 2000-09-26 | The Regents Of The University Of California | Long wavelength engineered fluorescent proteins |
| US6747137B1 (en) * | 1998-02-13 | 2004-06-08 | Genome Therapeutics Corporation | Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics |
-
2003
- 2003-04-02 US US10/406,618 patent/US7335488B2/en not_active Expired - Lifetime
- 2003-04-04 WO PCT/GB2003/001508 patent/WO2003087150A2/en not_active Ceased
- 2003-04-04 AT AT03715128T patent/ATE480556T1/en not_active IP Right Cessation
- 2003-04-04 DE DE60334087T patent/DE60334087D1/en not_active Expired - Lifetime
- 2003-04-04 EP EP03715128A patent/EP1492810B1/en not_active Expired - Lifetime
- 2003-04-04 AU AU2003219319A patent/AU2003219319A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE60334087D1 (en) | 2010-10-21 |
| EP1492810A2 (en) | 2005-01-05 |
| US20030219814A1 (en) | 2003-11-27 |
| AU2003219319A1 (en) | 2003-10-27 |
| WO2003087150A3 (en) | 2004-02-12 |
| WO2003087150A2 (en) | 2003-10-23 |
| US7335488B2 (en) | 2008-02-26 |
| EP1492810B1 (en) | 2010-09-08 |
| AU2003219319A8 (en) | 2003-10-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |