ATE480639T1 - Neues format zur detektion von heissstart- und echtzeit-polymerasekettenreaktion - Google Patents

Neues format zur detektion von heissstart- und echtzeit-polymerasekettenreaktion

Info

Publication number
ATE480639T1
ATE480639T1 AT04017701T AT04017701T ATE480639T1 AT E480639 T1 ATE480639 T1 AT E480639T1 AT 04017701 T AT04017701 T AT 04017701T AT 04017701 T AT04017701 T AT 04017701T AT E480639 T1 ATE480639 T1 AT E480639T1
Authority
AT
Austria
Prior art keywords
label
detecting
entity
polymera
real
Prior art date
Application number
AT04017701T
Other languages
English (en)
Inventor
Dieter Heindl
Waltraud Ankenbauer
Frank Laue
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE480639T1 publication Critical patent/ATE480639T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT04017701T 2003-08-01 2004-07-27 Neues format zur detektion von heissstart- und echtzeit-polymerasekettenreaktion ATE480639T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03016669A EP1502958A1 (de) 2003-08-01 2003-08-01 Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion

Publications (1)

Publication Number Publication Date
ATE480639T1 true ATE480639T1 (de) 2010-09-15

Family

ID=33522277

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04017701T ATE480639T1 (de) 2003-08-01 2004-07-27 Neues format zur detektion von heissstart- und echtzeit-polymerasekettenreaktion

Country Status (7)

Country Link
US (2) US20050037410A1 (de)
EP (1) EP1502958A1 (de)
JP (1) JP2005052143A (de)
AT (1) ATE480639T1 (de)
CA (1) CA2472970C (de)
DE (1) DE602004028993D1 (de)
ES (1) ES2350525T3 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2452318T3 (es) * 2006-12-21 2014-03-31 F. Hoffmann-La Roche Ag Método para la detección de AMPc y GMPc
US9111285B2 (en) 2007-08-27 2015-08-18 Qurio Holdings, Inc. System and method for representing content, user presence and interaction within virtual world advertising environments
CA2688174C (en) * 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
CN102549154A (zh) * 2009-07-17 2012-07-04 东洋制罐株式会社 核酸扩增方法
KR20110050327A (ko) * 2009-11-07 2011-05-13 주식회사 씨젠 Ｔｈｄ 프라이머 타겟 검출
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
JP6402905B2 (ja) * 2014-08-08 2018-10-10 東洋紡株式会社 核酸増幅の非特異反応を抑制する新規の方法
CN109207568A (zh) * 2017-06-30 2019-01-15 中国科学院上海巴斯德研究所 用于检测突变体的荧光实时检测试剂及方法
CN107254553A (zh) * 2017-06-30 2017-10-17 中国科学院上海巴斯德研究所 用于检测多种病原体的荧光实时检测方法及应用
CN107164565A (zh) * 2017-07-18 2017-09-15 张梦玲 一种用于以实时荧光rt‑pcr检测样品中hiv‑1病毒的引物对和包含其的试剂盒
CN110592201A (zh) * 2019-10-30 2019-12-20 上海千履基因科技有限公司 一管式高特异性核酸产物的pcr扩增方法、核酸快速检测方法和核酸检测试纸条
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
AU733647B2 (en) * 1996-05-15 2001-05-17 Biogenex Laboratories Non-nucleotide linking reagents
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
EP0930370B1 (de) * 1997-12-15 2007-08-08 CSL Behring GmbH Markierter Primer, geeignet für die Detektion von Nukleinsäuren
US6391551B1 (en) * 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
ATE286981T1 (de) * 1999-09-28 2005-01-15 Roche Diagnostics Gmbh Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
AUPR050700A0 (en) * 2000-10-03 2000-10-26 Id+Plus Ltd Detection method
JPWO2002077282A1 (ja) * 2001-03-27 2004-07-15 国際試薬株式会社 遺伝子検出方法
EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
EP1277841B1 (de) * 2001-07-11 2009-08-26 Roche Diagnostics GmbH Neue Zusammensetzung und Methode zur 'hot start' Nukleinsäureamplifizierung
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same

Also Published As

Publication number Publication date
CA2472970A1 (en) 2005-02-01
CA2472970C (en) 2012-05-01
US20090181401A1 (en) 2009-07-16
JP2005052143A (ja) 2005-03-03
US20050037410A1 (en) 2005-02-17
EP1502958A1 (de) 2005-02-02
DE602004028993D1 (de) 2010-10-21
ES2350525T3 (es) 2011-01-24

Similar Documents

Publication Publication Date Title
JP6966681B2 (ja) 限られたヌクレオチド組成を有するプライマーを用いた増幅
US8252558B2 (en) Methods for amplifying and detecting nucleic acid sequences
DE60140487D1 (en) Asynchrones pcr priming
DK0912766T3 (da) Overvågning af hybridisering under PCR
ATE512219T1 (de) Verfahren zur sauberen klonierung
KR101424192B1 (ko) 블로킹 올리고뉴클레오티드를 포함하는 핫스타트 ｐｃｒ용 조성물
KR970028548A (ko) 핵산의 형광 편광도 검출방법
JP2004506431A5 (de)
CN107446919B (zh) 一种恒温条件下合成核酸的方法及试剂盒
ATE480639T1 (de) Neues format zur detektion von heissstart- und echtzeit-polymerasekettenreaktion
CN105164279A (zh) 靶核酸的多重分析
CN109642251B (zh) 核酸扩增过程中的改进或与核酸扩增过程相关的改进
HK1247255A1 (zh) 底数大於2的指数核酸扩增
CN105154556A (zh) 实时荧光恒温指数扩增方法
US8198026B2 (en) Method of detecting specific fragments of DNA or RNA with the aid of a real-time polymerase chain reaction
WO2002061124A3 (en) Analysis of methylation patterns in genomic dna using bisulfite treatment and fluorescence polarisation assay techniques
JP2003506068A5 (de)
DE50214183D1 (de) Verfahren zum nachweis von in einer polymerase-kettenreaktion amplifizierten nukleinsäuremolekülen
CN108642147A (zh) 一种快速扩增核酸的方法
ATE287969T1 (de) Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung
WO1999029905A3 (en) Method for detecting apoptosis using fret labeled oligonucleotides
RU2414510C2 (ru) Способ амплификации специфичных фрагментов нуклеиновых кислот с помощью рекуррентной цепной реакции
RU2005104636A (ru) Способ проведения пцр
HK1254039A1 (zh) 用於催化性测定的方法和组合物
RU2003133652A (ru) Способ детекции микроорганизмов с помощью изотермической реакции амплификации-ера (экзонуклеазно-полимеразная амплификация (exonuclease- polimerase amplification)

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties