ATE484583T1 - Rekombinanter vektor zum löschen spezifischer chromosomenregionen und verfahren zum löschen spezifischer chromosomenregionen eines mikroorganismuschromosoms damit - Google Patents
Rekombinanter vektor zum löschen spezifischer chromosomenregionen und verfahren zum löschen spezifischer chromosomenregionen eines mikroorganismuschromosoms damitInfo
- Publication number
- ATE484583T1 ATE484583T1 AT08167449T AT08167449T ATE484583T1 AT E484583 T1 ATE484583 T1 AT E484583T1 AT 08167449 T AT08167449 T AT 08167449T AT 08167449 T AT08167449 T AT 08167449T AT E484583 T1 ATE484583 T1 AT E484583T1
- Authority
- AT
- Austria
- Prior art keywords
- recombinant vector
- deleting specific
- regions
- chromosome
- specific chromosome
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 210000000349 chromosome Anatomy 0.000 title 3
- 244000005700 microbiome Species 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 238000012217 deletion Methods 0.000 abstract 3
- 230000037430 deletion Effects 0.000 abstract 3
- 230000002759 chromosomal effect Effects 0.000 abstract 2
- 102000004533 Endonucleases Human genes 0.000 abstract 1
- 108010042407 Endonucleases Proteins 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020080012377A KR100958096B1 (ko) | 2008-02-11 | 2008-02-11 | 염색체 특정 부위 제거용 재조합 벡터 및 이를 이용한미생물 내 염색체 특정 부위의 제거방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE484583T1 true ATE484583T1 (de) | 2010-10-15 |
Family
ID=40220697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08167449T ATE484583T1 (de) | 2008-02-11 | 2008-10-23 | Rekombinanter vektor zum löschen spezifischer chromosomenregionen und verfahren zum löschen spezifischer chromosomenregionen eines mikroorganismuschromosoms damit |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20090305421A1 (de) |
| EP (1) | EP2088195B1 (de) |
| JP (1) | JP4795415B2 (de) |
| KR (1) | KR100958096B1 (de) |
| AT (1) | ATE484583T1 (de) |
| DE (1) | DE602008002995D1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150353940A1 (en) * | 2013-08-05 | 2015-12-10 | Absci, Llc | Vectors for use in an inducible coexpression system |
| CN104513829B (zh) * | 2013-09-27 | 2018-03-06 | 中国科学院上海生命科学研究院 | 基因组中目的基因的遗传改造方法 |
| CN104894046A (zh) * | 2015-05-29 | 2015-09-09 | 暨南大学 | Hsv-1病毒体外基因敲除系统及其制备方法与应用 |
| CN113462712B (zh) * | 2021-07-14 | 2023-11-24 | 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） | 一种温控自剪切单质粒同源重组系统及其在基因编辑中的应用 |
| CN116589542B (zh) * | 2023-07-04 | 2023-09-15 | 四川大学华西医院 | 基于AmpR蛋白突变的转录因子、启动子及构建方法 |
| WO2025106720A2 (en) * | 2023-11-15 | 2025-05-22 | The Administrators Of The Tulane Educational Fund | Enhanced prokaryotic gene expression regulators through nucleotide-level mapping |
-
2008
- 2008-02-11 KR KR1020080012377A patent/KR100958096B1/ko not_active Expired - Fee Related
- 2008-10-16 JP JP2008267968A patent/JP4795415B2/ja not_active Expired - Fee Related
- 2008-10-23 DE DE602008002995T patent/DE602008002995D1/de active Active
- 2008-10-23 AT AT08167449T patent/ATE484583T1/de not_active IP Right Cessation
- 2008-10-23 EP EP08167449A patent/EP2088195B1/de not_active Not-in-force
- 2008-11-04 US US12/291,007 patent/US20090305421A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2009183279A (ja) | 2009-08-20 |
| EP2088195A1 (de) | 2009-08-12 |
| EP2088195B1 (de) | 2010-10-13 |
| KR100958096B1 (ko) | 2010-05-14 |
| US20090305421A1 (en) | 2009-12-10 |
| DE602008002995D1 (de) | 2010-11-25 |
| JP4795415B2 (ja) | 2011-10-19 |
| KR20090086870A (ko) | 2009-08-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |