ATE486950T1 - AUTOMATIC METHOD FOR DETECTING PATHOGENIC ORGANISMS IN WATER - Google Patents

AUTOMATIC METHOD FOR DETECTING PATHOGENIC ORGANISMS IN WATER

Info

Publication number
ATE486950T1
ATE486950T1 AT02758725T AT02758725T ATE486950T1 AT E486950 T1 ATE486950 T1 AT E486950T1 AT 02758725 T AT02758725 T AT 02758725T AT 02758725 T AT02758725 T AT 02758725T AT E486950 T1 ATE486950 T1 AT E486950T1
Authority
AT
Austria
Prior art keywords
pathogenic organisms
unit
rna
detecting
concentrating
Prior art date
Application number
AT02758725T
Other languages
German (de)
Inventor
Marie-Christine Huau
Thomas Schalkhammer
Original Assignee
Amenagement Urbain & Rural
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amenagement Urbain & Rural filed Critical Amenagement Urbain & Rural
Priority claimed from PCT/IB2002/003424 external-priority patent/WO2003010278A2/en
Application granted granted Critical
Publication of ATE486950T1 publication Critical patent/ATE486950T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00564—Handling or washing solid phase elements, e.g. beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
    • G01N35/109—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with two horizontal degrees of freedom

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Geophysics And Detection Of Objects (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

Detecting the presence of pathogenic organisms in water sample, involves concentrating pathogenic organisms, breaking to open cells of pathogenic organisms, concentrating DNA and/or RNA, amplifying nucleic acid and detecting DNA and/or RNA by qualitatively and/or quantitatively. The process is controlled fully automatically by using a control logic system. An Independent claim is included for a device for detecting the presence of pathogenic organisms in a water sample. The device comprises a unit (1) for concentrating the pathogenic organisms, a unit (2) for breaking open cells from the pathogenic organisms, a unit (3) for concentrating DNA and/or RNA, a unit (4) for amplifying nucleic acids, a unit (5) for qualitatively and/or quantitatively detecting DNA and/or RNA and a control logic system (6) which controls fully automatically the sequential treatment steps carried out by units (1) to (5), pumping units (7), automatic sampling and distribution units (8), and a central control system (9).
AT02758725T 2001-07-24 2002-07-24 AUTOMATIC METHOD FOR DETECTING PATHOGENIC ORGANISMS IN WATER ATE486950T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30717101P 2001-07-24 2001-07-24
FR0109841A FR2827873B1 (en) 2001-07-24 2001-07-24 AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER
PCT/IB2002/003424 WO2003010278A2 (en) 2001-07-24 2002-07-24 Automated process for detecting pathogenic organisms in water

Publications (1)

Publication Number Publication Date
ATE486950T1 true ATE486950T1 (en) 2010-11-15

Family

ID=8865828

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02758725T ATE486950T1 (en) 2001-07-24 2002-07-24 AUTOMATIC METHOD FOR DETECTING PATHOGENIC ORGANISMS IN WATER

Country Status (3)

Country Link
AT (1) ATE486950T1 (en)
DE (1) DE60238199D1 (en)
FR (1) FR2827873B1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2868082B1 (en) * 2004-03-26 2006-06-09 Amenagement Urbain & Rural PROCESS FOR EXTRACTING INTRACELLULAR COMPOUNDS OR COMPLEXES FROM A SAMPLE CONTAINING CELLS IN SUSPENSION IN A LIQUID
CN106929406B (en) * 2017-05-23 2024-02-06 至农科技发展(浙江)有限公司 Compact device for nucleic acid extraction and detection
CN116583609A (en) * 2020-10-21 2023-08-11 生物辐射实验室股份有限公司 Detection of Pathogens in Wastewater

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2033718A1 (en) * 1990-01-19 1991-07-20 F. Hoffmann-La Roche Ag Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor
ATE291637T1 (en) * 1997-03-24 2005-04-15 Robert E Fields BIOMOLECULAR PROCESSOR
WO1999018421A1 (en) * 1997-10-03 1999-04-15 Monterey Bay Aquarium Research Institute Aquatic autosampler device
JP3623479B2 (en) * 1999-06-22 2005-02-23 テカン トレーディング アーゲー Apparatus and method for performing miniaturized in vitro amplification assays
US6878517B1 (en) * 1999-12-15 2005-04-12 Congra Grocery Products Company Multispecies food testing and characterization organoleptic properties

Also Published As

Publication number Publication date
FR2827873A1 (en) 2003-01-31
FR2827873B1 (en) 2003-09-26
DE60238199D1 (en) 2010-12-16

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