ATE489479T1 - Nukleinsäureamplifikation - Google Patents
NukleinsäureamplifikationInfo
- Publication number
- ATE489479T1 ATE489479T1 AT03815905T AT03815905T ATE489479T1 AT E489479 T1 ATE489479 T1 AT E489479T1 AT 03815905 T AT03815905 T AT 03815905T AT 03815905 T AT03815905 T AT 03815905T AT E489479 T1 ATE489479 T1 AT E489479T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- terminal
- phosphate
- nucleotides
- detectable
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 229910019142 PO4 Inorganic materials 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 239000010452 phosphate Substances 0.000 abstract 2
- 230000004544 DNA amplification Effects 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 229920000388 Polyphosphate Polymers 0.000 abstract 1
- 230000004913 activation Effects 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract 1
- 235000021317 phosphate Nutrition 0.000 abstract 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 abstract 1
- 239000001205 polyphosphate Substances 0.000 abstract 1
- 235000011176 polyphosphates Nutrition 0.000 abstract 1
- 239000003381 stabilizer Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US44527403P | 2003-02-05 | 2003-02-05 | |
| PCT/US2003/027287 WO2004072304A1 (en) | 2003-02-05 | 2003-08-29 | Nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE489479T1 true ATE489479T1 (de) | 2010-12-15 |
Family
ID=32869330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03815905T ATE489479T1 (de) | 2003-02-05 | 2003-08-29 | Nukleinsäureamplifikation |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7125671B2 (de) |
| EP (1) | EP1590479B1 (de) |
| JP (1) | JP2006513705A (de) |
| AT (1) | ATE489479T1 (de) |
| AU (1) | AU2003265857A1 (de) |
| DE (1) | DE60335144D1 (de) |
| ES (1) | ES2355555T3 (de) |
| WO (1) | WO2004072304A1 (de) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936702B2 (en) * | 2000-06-07 | 2005-08-30 | Li-Cor, Inc. | Charge-switch nucleotides |
| US20030064366A1 (en) * | 2000-07-07 | 2003-04-03 | Susan Hardin | Real-time sequence determination |
| EP1354064A2 (de) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen |
| US7668697B2 (en) | 2006-02-06 | 2010-02-23 | Andrei Volkov | Method for analyzing dynamic detectable events at the single molecule level |
| US7727722B2 (en) * | 2001-08-29 | 2010-06-01 | General Electric Company | Ligation amplification |
| CA2513690A1 (en) * | 2003-02-05 | 2004-08-26 | Amersham Biosciences Corp | Solid phase sequencing |
| WO2006034036A1 (en) | 2004-09-16 | 2006-03-30 | Applera Corporation | Fluorescent dye compounds, conjugates and uses thereof |
| WO2007069717A1 (ja) * | 2005-12-15 | 2007-06-21 | Katsuya Honda | Ｄｎａ断片を増幅するためのｄｎａの増幅方法及び鋳型ｄｎａの活性化方法 |
| US20080076189A1 (en) * | 2006-03-30 | 2008-03-27 | Visigen Biotechnologies, Inc. | Modified surfaces for the detection of biomolecules at the single molecule level |
| US7563574B2 (en) * | 2006-03-31 | 2009-07-21 | Pacific Biosciences Of California, Inc. | Methods, systems and compositions for monitoring enzyme activity and applications thereof |
| FR2906532B1 (fr) * | 2006-09-28 | 2008-12-12 | Biomerieux Sa | Nouvel oligonucleotide marque |
| US20100227327A1 (en) * | 2008-08-08 | 2010-09-09 | Xiaoliang Sunney Xie | Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides |
| US20100036110A1 (en) * | 2008-08-08 | 2010-02-11 | Xiaoliang Sunney Xie | Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides |
| US20100255487A1 (en) | 2009-03-27 | 2010-10-07 | Life Technologies Corporation | Methods and apparatus for single molecule sequencing using energy transfer detection |
| US8632975B2 (en) | 2009-06-05 | 2014-01-21 | Life Technologies Corporation | Nucleotide transient binding for sequencing methods |
| ES2351294B8 (es) * | 2009-07-02 | 2012-06-06 | Consejo Superior De Investigaciones Científicas (Csic) | Metodo para la replicación, amplificación, o secuenciación de un adn molde. |
| KR101190792B1 (ko) * | 2010-08-16 | 2012-10-12 | 한국과학기술연구원 | 변형 뉴클레오티드 및 이를 이용한 실시간 중합효소 반응 |
| WO2012092403A1 (en) | 2010-12-29 | 2012-07-05 | Life Technologies Corporation | Ddao compounds as fluorescent reference standards |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| EP3665274B1 (de) | 2017-08-07 | 2025-10-15 | DNA Script | Varianten der familie-a-dna-polymerase und verwendungen davon |
| WO2019135007A1 (en) | 2018-01-08 | 2019-07-11 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| EP3826762A1 (de) | 2018-07-23 | 2021-06-02 | DNA Script | Massiv parallele enzymatische synthese von nukleinsäuresträngen |
| CN113423841A (zh) | 2018-12-13 | 2021-09-21 | Dna斯克瑞普特公司 | 细胞和生物分子上的直接寡核苷酸合成 |
| EP3924500A1 (de) | 2019-02-12 | 2021-12-22 | DNA Script | Effiziente produktspaltung in der template-freien enzymatischen synthese von polynukleotiden |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5849478A (en) * | 1986-08-14 | 1998-12-15 | Cashman; Daniel P. | Blocked-polymerase polynucleotide immunoassay method and kit |
| WO2000036151A1 (en) * | 1998-12-14 | 2000-06-22 | Li-Cor, Inc. | A heterogeneous assay for pyrophosphate detection |
| US7056661B2 (en) * | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| US6399335B1 (en) * | 1999-11-16 | 2002-06-04 | Advanced Research And Technology Institute, Inc. | γ-phosphoester nucleoside triphosphates |
| WO2001094609A1 (en) * | 2000-06-07 | 2001-12-13 | Li-Cor, Inc. | Charge-switch nucleotides |
| US20030064366A1 (en) * | 2000-07-07 | 2003-04-03 | Susan Hardin | Real-time sequence determination |
| EP1354064A2 (de) * | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen |
| US7033762B2 (en) * | 2001-08-29 | 2006-04-25 | Amersham Biosciences Corp | Single nucleotide amplification and detection by polymerase |
| US7052839B2 (en) * | 2001-08-29 | 2006-05-30 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
| ATE458067T1 (de) * | 2001-08-29 | 2010-03-15 | Ge Healthcare Bio Sciences | Markierte nukleosidpolyphosphate |
-
2003
- 2003-08-29 DE DE60335144T patent/DE60335144D1/de not_active Expired - Lifetime
- 2003-08-29 AU AU2003265857A patent/AU2003265857A1/en not_active Abandoned
- 2003-08-29 US US10/651,362 patent/US7125671B2/en not_active Expired - Lifetime
- 2003-08-29 WO PCT/US2003/027287 patent/WO2004072304A1/en not_active Ceased
- 2003-08-29 ES ES03815905T patent/ES2355555T3/es not_active Expired - Lifetime
- 2003-08-29 JP JP2004568296A patent/JP2006513705A/ja active Pending
- 2003-08-29 EP EP03815905A patent/EP1590479B1/de not_active Expired - Lifetime
- 2003-08-29 AT AT03815905T patent/ATE489479T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003265857A1 (en) | 2004-09-06 |
| EP1590479B1 (de) | 2010-11-24 |
| JP2006513705A (ja) | 2006-04-27 |
| EP1590479A1 (de) | 2005-11-02 |
| US7125671B2 (en) | 2006-10-24 |
| WO2004072304A1 (en) | 2004-08-26 |
| ES2355555T3 (es) | 2011-03-28 |
| DE60335144D1 (de) | 2011-01-05 |
| US20040152104A1 (en) | 2004-08-05 |
| EP1590479A4 (de) | 2006-04-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |