ATE494370T1 - Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren - Google Patents

Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren

Info

Publication number
ATE494370T1
ATE494370T1 AT03748973T AT03748973T ATE494370T1 AT E494370 T1 ATE494370 T1 AT E494370T1 AT 03748973 T AT03748973 T AT 03748973T AT 03748973 T AT03748973 T AT 03748973T AT E494370 T1 ATE494370 T1 AT E494370T1
Authority
AT
Austria
Prior art keywords
nucleic acids
transposon
integration
based vectors
compositions
Prior art date
Application number
AT03748973T
Other languages
English (en)
Inventor
Joseph Kaminski
Original Assignee
Manoa Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Manoa Biosciences Inc filed Critical Manoa Biosciences Inc
Application granted granted Critical
Publication of ATE494370T1 publication Critical patent/ATE494370T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03748973T 2002-07-24 2003-07-24 Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren ATE494370T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39862802P 2002-07-24 2002-07-24
PCT/US2003/023090 WO2004009792A2 (en) 2002-07-24 2003-07-24 Transposon-based vectors and methods of nucleic acid integration

Publications (1)

Publication Number Publication Date
ATE494370T1 true ATE494370T1 (de) 2011-01-15

Family

ID=30771227

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03748973T ATE494370T1 (de) 2002-07-24 2003-07-24 Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren

Country Status (9)

Country Link
US (1) US20060210977A1 (de)
EP (1) EP1546322B1 (de)
AT (1) ATE494370T1 (de)
AU (1) AU2003268022B2 (de)
CA (1) CA2533708C (de)
DE (1) DE60335637D1 (de)
ES (1) ES2358994T3 (de)
SI (1) SI1546322T1 (de)
WO (1) WO2004009792A2 (de)

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US20040172667A1 (en) 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20060236413A1 (en) * 2003-02-10 2006-10-19 Zoltan Ivics Transposon-based targeting system
ATE498016T1 (de) * 2003-02-10 2011-02-15 Max Delbrueck Centrum Transposon-system zur gezielten integration
US8420386B2 (en) * 2003-02-10 2013-04-16 Max-Delbruck-Centrum Fur Molekulare Medizin (Mdc) Transposon-based targeting system
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
WO2005089123A2 (en) * 2004-03-04 2005-09-29 Massachusetts Institute Of Technology Therapeutic anti-cancer dna
EP1755640A4 (de) * 2004-04-30 2009-10-28 Poetic Genetics Llc Hybridrekombinasen für die genom-manipulation
US7470777B2 (en) 2004-12-22 2008-12-30 Iowa State University Research Foundation, Inc. Compositions and methods related to modified retroviral vectors for restricted, site specific integration
US7919583B2 (en) 2005-08-08 2011-04-05 Discovery Genomics, Inc. Integration-site directed vector systems
WO2008100424A2 (en) * 2007-02-09 2008-08-21 University Of Hawaii Animals and cells with genomic target sites for transposase-mediated transgenesis
US20110130444A1 (en) * 2007-05-04 2011-06-02 Stefan Moisyadi Methods and compositions for targeted delivery of gene therapeutic vectors
US20090042297A1 (en) * 2007-06-01 2009-02-12 George Jr Alfred L Piggybac transposon-based vectors and methods of nucleic acid integration
US20100304445A1 (en) * 2007-06-16 2010-12-02 Waclaw Szybalski Nucleic acid packaging system
CN101503670B (zh) * 2008-02-04 2011-12-14 复旦大学附属华山医院 一种含有整合子的工程菌株
WO2010008562A2 (en) * 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
WO2010036978A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
WO2010085699A2 (en) * 2009-01-23 2010-07-29 The Johns Hopkins University Mammalian piggybac transposon and methods of use
GB2467167B (en) * 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
EP2417263B1 (de) 2009-04-09 2015-09-23 ProteoVec Holding L.L.C. Herstellung von proteinen unter verwendung von vektoren auf transposonbasis
WO2012081628A1 (ja) * 2010-12-15 2012-06-21 大学共同利用機関法人情報・システム研究機構 タンパク質の生産方法
EP3019618B1 (de) * 2013-07-12 2018-10-31 University of South Alabama Minimale piggybac-vektoren für genomische integration
AU2016325384B2 (en) * 2015-09-22 2021-07-22 Julius-Maximilians-Universitat Wurzburg A method for high level and stable gene transfer in lymphocytes
CN105154473B (zh) * 2015-09-30 2019-03-01 上海细胞治疗研究院 一种高效安全的转座子整合系统及其用途
CN105481984B (zh) * 2015-12-03 2020-09-22 上海细胞治疗研究院 一种高效介导外源基因整合的转座酶及其用途
JP2021519073A (ja) 2018-03-29 2021-08-10 ジェネンテック， インコーポレイテッド 哺乳動物細胞におけるラクトジェニック活性の制御
JP2021530212A (ja) * 2018-07-13 2021-11-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California レトロトランスポゾンベースの送達媒体及びその使用方法
HUE068630T2 (hu) 2018-12-10 2025-01-28 Amgen Inc Mutáns PiggyBac transzpozáz
WO2020164702A1 (en) * 2019-02-13 2020-08-20 Probiogen Ag Transposase with enhanced insertion site selection properties
KR102990253B1 (ko) * 2019-06-11 2026-07-10 우니베르시타트 폼페우 파브라 표적화된 유전자 편집 작제물 및 이의 사용 방법
EP3835421A1 (de) * 2019-12-11 2021-06-16 Consejo Superior de Investigaciones Científicas (CSIC) Vektoren für gewebespezifische transkriptomik
KR102937659B1 (ko) 2020-02-19 2026-03-10 우시 바이올로직스 아일랜드 리미티드 강화된 발현 시스템 및 그 사용방법
US12098365B2 (en) 2020-03-26 2024-09-24 Genentech, Inc. Modified mammalian cells
EP4146798A4 (de) 2020-05-04 2025-02-12 Saliogen Therapeutics, Inc. Transpositionsbasierte therapien
EP4172192A1 (de) 2020-06-24 2023-05-03 Genentech, Inc. Apoptoseresistente zelllinien
CA3173696A1 (en) 2020-07-17 2022-01-20 Volker Sandig Hyperactive transposons and transposases
JP2024514222A (ja) 2021-04-19 2024-03-28 ジェネンテック， インコーポレイテッド 改変された哺乳動物細胞
CN117396599A (zh) 2021-05-21 2024-01-12 基因泰克公司 用于生产目的重组产物的经修饰的细胞
CN118119704A (zh) * 2021-09-08 2024-05-31 宏基因组学公司 用于转座货物核苷酸序列的系统和方法
CN119731330A (zh) 2021-12-22 2025-03-28 基因泰克公司 多载体重组酶介导的盒式交换
KR20250078960A (ko) * 2022-09-29 2025-06-04 브이아이비 브이지더블유 대규모 직교성 cre-매개 재조합을 위한 신규 loxpsym 부위

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WO1997020038A1 (en) * 1995-12-01 1997-06-05 The Regents Of The University Of California Compositions and methods for site-directed integration into dna
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WO2001030965A2 (en) * 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
IL154059A0 (en) * 2000-07-21 2003-07-31 Syngenta Participations Ag Zinc finger domain recognition code and uses thereof
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EP1308516A1 (de) * 2001-10-24 2003-05-07 ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire Zielgerichtete Rekombinase-Fusionsproteine und entsprechende Polynukleotide, Vektoren und Kits, und deren Verwendungen zur zielgerichteten DNS-Rekombination

Also Published As

Publication number Publication date
CA2533708A1 (en) 2004-01-29
EP1546322A4 (de) 2006-07-19
EP1546322A2 (de) 2005-06-29
SI1546322T1 (sl) 2011-05-31
DE60335637D1 (de) 2011-02-17
AU2003268022B2 (en) 2009-02-05
WO2004009792A3 (en) 2004-05-13
AU2003268022A1 (en) 2004-02-09
EP1546322B1 (de) 2011-01-05
WO2004009792A2 (en) 2004-01-29
ES2358994T3 (es) 2011-05-17
US20060210977A1 (en) 2006-09-21
CA2533708C (en) 2013-05-14

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