ATE494370T1 - Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren - Google Patents

Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren

Info

Publication number
ATE494370T1
ATE494370T1 AT03748973T AT03748973T ATE494370T1 AT E494370 T1 ATE494370 T1 AT E494370T1 AT 03748973 T AT03748973 T AT 03748973T AT 03748973 T AT03748973 T AT 03748973T AT E494370 T1 ATE494370 T1 AT E494370T1
Authority
AT
Austria
Prior art keywords
nucleic acids
transposon
integration
based vectors
compositions
Prior art date
Application number
AT03748973T
Other languages
English (en)
Inventor
Joseph Kaminski
Original Assignee
Manoa Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Manoa Biosciences Inc filed Critical Manoa Biosciences Inc
Application granted granted Critical
Publication of ATE494370T1 publication Critical patent/ATE494370T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03748973T 2002-07-24 2003-07-24 Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren ATE494370T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39862802P 2002-07-24 2002-07-24
PCT/US2003/023090 WO2004009792A2 (en) 2002-07-24 2003-07-24 Transposon-based vectors and methods of nucleic acid integration

Publications (1)

Publication Number Publication Date
ATE494370T1 true ATE494370T1 (de) 2011-01-15

Family

ID=30771227

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03748973T ATE494370T1 (de) 2002-07-24 2003-07-24 Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren

Country Status (9)

Country Link
US (1) US20060210977A1 (de)
EP (1) EP1546322B1 (de)
AT (1) ATE494370T1 (de)
AU (1) AU2003268022B2 (de)
CA (1) CA2533708C (de)
DE (1) DE60335637D1 (de)
ES (1) ES2358994T3 (de)
SI (1) SI1546322T1 (de)
WO (1) WO2004009792A2 (de)

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US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040172667A1 (en) 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
ATE536419T1 (de) * 2003-02-10 2011-12-15 Max Delbrueck Centrum Transposon basiertes targeting- system
WO2004069994A2 (en) * 2003-02-10 2004-08-19 Max-Delbrück-Centrum Für Molekulare Medizin (Mdc) Transposon-based targeting system
JP2006517104A (ja) * 2003-02-10 2006-07-20 マックス−デルブルック−セントラム フユール モレクラーレ メディツィン（エムディーシー） トランスポゾンに基づく標的化システム
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
EP1730286A4 (de) * 2004-03-04 2007-09-05 Massachusetts Inst Technology Therapeutische antikrebs-dna
JP4769796B2 (ja) * 2004-04-30 2011-09-07 ポエティック ジェネティックス エルエルシー ゲノム操作のためのハイブリッド型リコンビナーゼ
US7470777B2 (en) 2004-12-22 2008-12-30 Iowa State University Research Foundation, Inc. Compositions and methods related to modified retroviral vectors for restricted, site specific integration
US7919583B2 (en) 2005-08-08 2011-04-05 Discovery Genomics, Inc. Integration-site directed vector systems
WO2008100424A2 (en) * 2007-02-09 2008-08-21 University Of Hawaii Animals and cells with genomic target sites for transposase-mediated transgenesis
US20110130444A1 (en) * 2007-05-04 2011-06-02 Stefan Moisyadi Methods and compositions for targeted delivery of gene therapeutic vectors
US20090042297A1 (en) * 2007-06-01 2009-02-12 George Jr Alfred L Piggybac transposon-based vectors and methods of nucleic acid integration
JP2010529862A (ja) * 2007-06-16 2010-09-02 スカラブ ゲノミクス， エルエルシー 核酸パッケージングシステム
CN101503670B (zh) * 2008-02-04 2011-12-14 复旦大学附属华山医院 一种含有整合子的工程菌株
US8309791B2 (en) * 2008-07-16 2012-11-13 Recombinectics, Inc. Method for producing a transgenic pig using a hyper-methylated transposon
WO2010036978A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
WO2010085699A2 (en) * 2009-01-23 2010-07-29 The Johns Hopkins University Mammalian piggybac transposon and methods of use
GB2467167B (en) * 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
WO2010118360A1 (en) 2009-04-09 2010-10-14 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Production of proteins using transposon-based vectors
PT2653540T (pt) * 2010-12-15 2018-02-27 Kyowa Hakko Kirin Co Ltd Processo de produção de proteínas
EP3019618B1 (de) 2013-07-12 2018-10-31 University of South Alabama Minimale piggybac-vektoren für genomische integration
CN108601849A (zh) * 2015-09-22 2018-09-28 尤利乌斯·马克西米利安维尔茨堡大学 一种在淋巴细胞中高水平的和稳定的基因转移的方法
CN105154473B (zh) * 2015-09-30 2019-03-01 上海细胞治疗研究院 一种高效安全的转座子整合系统及其用途
CN105481984B (zh) * 2015-12-03 2020-09-22 上海细胞治疗研究院 一种高效介导外源基因整合的转座酶及其用途
JP2021519073A (ja) 2018-03-29 2021-08-10 ジェネンテック， インコーポレイテッド 哺乳動物細胞におけるラクトジェニック活性の制御
JP2021530212A (ja) * 2018-07-13 2021-11-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California レトロトランスポゾンベースの送達媒体及びその使用方法
WO2020123327A1 (en) 2018-12-10 2020-06-18 Amgen Inc. Mutated piggybac transposase
US20220090142A1 (en) * 2019-02-13 2022-03-24 Probiogen Ag Transposase with enhanced insertion site selection properties
AU2020290790B2 (en) * 2019-06-11 2026-02-19 Universitat Pompeu Fabra Targeted gene editing constructs and methods of using the same
EP3835421A1 (de) * 2019-12-11 2021-06-16 Consejo Superior de Investigaciones Científicas (CSIC) Vektoren für gewebespezifische transkriptomik
JP2023518841A (ja) 2020-03-26 2023-05-08 ジェネンテック， インコーポレイテッド 宿主細胞タンパク質が減少した修飾哺乳動物細胞
CN115698268A (zh) 2020-05-04 2023-02-03 萨利欧基因治疗公司 基于转座的疗法
WO2021262783A1 (en) 2020-06-24 2021-12-30 Genentech, Inc. Apoptosis resistant cell lines
KR20230038425A (ko) 2020-07-17 2023-03-20 프로바이오겐 아게 과활성 트랜스포손 및 트랜스포사제
JP2024514222A (ja) 2021-04-19 2024-03-28 ジェネンテック， インコーポレイテッド 改変された哺乳動物細胞
EP4341385A1 (de) 2021-05-21 2024-03-27 Genentech, Inc. Modifizierte zellen zur herstellung eines rekombinanten produkts von interesse
EP4399286A4 (de) * 2021-09-08 2025-09-03 Metagenomi Inc Systeme und verfahren zur transponierung von frachtnukleotidsequenzen
EP4453227A1 (de) 2021-12-22 2024-10-30 Genentech, Inc. Durch multivektorrekombinase vermittelter kassettenaustausch
WO2024068845A1 (en) * 2022-09-29 2024-04-04 Vib Vzw New loxpsym sites for large-scale orthogonal cre-mediated recombination

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EP0871711A1 (de) * 1995-12-01 1998-10-21 The Regents Of The University Of California Zusammensetzungen und methoden für die ortsspezifische integration in dns
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WO2001030965A2 (en) * 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
EP1303608A2 (de) * 2000-07-21 2003-04-23 Syngenta Participations AG Code zur erkennung von zinkfingerdomänen und dessen verwendungen
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EP1308516A1 (de) * 2001-10-24 2003-05-07 ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire Zielgerichtete Rekombinase-Fusionsproteine und entsprechende Polynukleotide, Vektoren und Kits, und deren Verwendungen zur zielgerichteten DNS-Rekombination

Also Published As

Publication number Publication date
EP1546322A2 (de) 2005-06-29
DE60335637D1 (de) 2011-02-17
SI1546322T1 (sl) 2011-05-31
AU2003268022B2 (en) 2009-02-05
WO2004009792A2 (en) 2004-01-29
AU2003268022A1 (en) 2004-02-09
CA2533708C (en) 2013-05-14
US20060210977A1 (en) 2006-09-21
CA2533708A1 (en) 2004-01-29
ES2358994T3 (es) 2011-05-17
WO2004009792A3 (en) 2004-05-13
EP1546322A4 (de) 2006-07-19
EP1546322B1 (de) 2011-01-05

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