ATE502103T1 - METHOD FOR OBTAINING A RECOMBINANT MAMMAL MYELOMA CELL LINE ADAPTED TO GROWTH IN SERUM-FREE AND PROTEIN-FREE MEDIA - Google Patents

METHOD FOR OBTAINING A RECOMBINANT MAMMAL MYELOMA CELL LINE ADAPTED TO GROWTH IN SERUM-FREE AND PROTEIN-FREE MEDIA

Info

Publication number
ATE502103T1
ATE502103T1 AT03757654T AT03757654T ATE502103T1 AT E502103 T1 ATE502103 T1 AT E502103T1 AT 03757654 T AT03757654 T AT 03757654T AT 03757654 T AT03757654 T AT 03757654T AT E502103 T1 ATE502103 T1 AT E502103T1
Authority
AT
Austria
Prior art keywords
free
serum
protein
growth
obtaining
Prior art date
Application number
AT03757654T
Other languages
German (de)
Inventor
Rodriguez Rolando Perez
Vitlloch Adolfo Castillo
Sarazola Svieta Vitores
Ayo Tammy Boggiano
Del Calvo Luis Rojas
Original Assignee
Centro Inmunologia Molecular
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Inmunologia Molecular filed Critical Centro Inmunologia Molecular
Application granted granted Critical
Publication of ATE502103T1 publication Critical patent/ATE502103T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Oncology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT03757654T 2002-10-23 2003-10-22 METHOD FOR OBTAINING A RECOMBINANT MAMMAL MYELOMA CELL LINE ADAPTED TO GROWTH IN SERUM-FREE AND PROTEIN-FREE MEDIA ATE502103T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU20020239A CU23097A1 (en) 2002-10-23 2002-10-23 METHOD FOR OBTAINING CELLULAR LINES IN PROTEIN FREE MEDIA AND CELL LINES OBTAINED BY THIS METHOD
PCT/CU2003/000012 WO2004038010A1 (en) 2002-10-23 2003-10-22 Method of obtaining cell lines in a protein-free medium and cell lines thus obtained

Publications (1)

Publication Number Publication Date
ATE502103T1 true ATE502103T1 (en) 2011-04-15

Family

ID=40134843

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03757654T ATE502103T1 (en) 2002-10-23 2003-10-22 METHOD FOR OBTAINING A RECOMBINANT MAMMAL MYELOMA CELL LINE ADAPTED TO GROWTH IN SERUM-FREE AND PROTEIN-FREE MEDIA

Country Status (27)

Country Link
US (2) US20060036082A1 (en)
EP (2) EP2363418B1 (en)
JP (1) JP4674087B2 (en)
KR (1) KR101186048B1 (en)
CN (1) CN1714147B (en)
AR (1) AR041645A1 (en)
AT (1) ATE502103T1 (en)
AU (1) AU2003273727B2 (en)
BR (1) BR0315565B1 (en)
CA (1) CA2503315C (en)
CU (1) CU23097A1 (en)
DE (1) DE60336415D1 (en)
DK (1) DK1564285T3 (en)
EA (1) EA007465B1 (en)
EC (1) ECSP055740A (en)
ES (2) ES2360049T3 (en)
GE (1) GEP20084444B (en)
HR (1) HRP20050760A2 (en)
LT (1) LT5354B (en)
LV (1) LV13361B (en)
MY (1) MY144088A (en)
NZ (1) NZ540171A (en)
PE (1) PE20040552A1 (en)
PT (1) PT1564285E (en)
TN (1) TNSN05116A1 (en)
UY (1) UY28037A1 (en)
WO (1) WO2004038010A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104673754B (en) * 2015-02-14 2018-03-02 百泰生物药业有限公司 Recombinate the screening technique of myeloma cell clone and thus obtained antibody molecule
CN104651314B (en) * 2015-02-14 2018-06-19 百泰生物药业有限公司 Obtain the method for high and stable yields expression cell clone and thus obtained antibody molecule

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3694263A (en) * 1970-10-23 1972-09-26 Joseph J Korn Sr Swimming pool cleaning methods and apparatus therefor
CU22545A1 (en) 1994-11-18 1999-03-31 Centro Inmunologia Molecular OBTAINING A CHEMICAL AND HUMANIZED ANTIBODY AGAINST THE RECEPTOR OF THE EPIDERMAL GROWTH FACTOR FOR DIAGNOSTIC AND THERAPEUTIC USE
US3886616A (en) * 1972-12-06 1975-06-03 Fay A Hayes Hand propelled swimming pool cleaner
US4275474A (en) * 1979-04-30 1981-06-30 Woodard Randle C Vacuum head for swimming pool cleaning system
US4240174A (en) * 1979-07-30 1980-12-23 Scott Jeffrey L Self-contained mobile pool cleaning apparatus
US4430214A (en) * 1982-09-15 1984-02-07 Baker Marvin E Strainer mill for swimming pool pump intake
AU2986484A (en) * 1983-06-27 1985-01-03 Monash University Leptospira strains grown in protein free medium
US4558479A (en) * 1984-01-26 1985-12-17 Alopex Industries, Inc. Pool cleaner
US4683599A (en) * 1984-03-30 1987-08-04 Fahet Nv Automatic pool cleaner fitting
US4581075A (en) * 1985-03-15 1986-04-08 Maxi-Sweep, Inc. Self-propelled water borne pool cleaner
US5170064A (en) 1989-09-29 1992-12-08 Atomic Energy Of Canada Limited Infrared-based gas detector using a cavity having elliptical reflecting surface
JP2696001B2 (en) 1991-04-15 1998-01-14 財団法人化学及血清療法研究所 Medium for recombinant protein production
EP0531562A1 (en) 1991-09-11 1993-03-17 Doerr, Hans-Wilhelm, Prof. Dr. med. Culturing of mammalian cells
WO1996008565A2 (en) * 1994-09-16 1996-03-21 Cancer Research Fund Of Contra Costa RECOMBINANT PEPTIDES DERIVED FROM THE Mc3 ANTI-BA46 ANTIBODY, METHODS OF USE THEREOF, AND METHODS OF HUMANIZING ANTIBODY PEPTIDES
ES2378409T3 (en) 1994-11-10 2012-04-12 Baxter Healthcare S.A. Method for producing biological substances in protein-free culture
DE69608668T2 (en) 1995-02-23 2001-02-01 Quest International B.V., Naarden PEPTIDES FOR TISSUE AND CELL CULTURE MEDIA
AUPN442295A0 (en) 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells
CU22584A1 (en) 1995-11-17 1999-11-03 Centro Inmunologia Molecular PHARMACEUTICAL COMPOSITIONS CONTAINING A MONOCLONAL ANTIBODY THAT RECOGNIZES THE CD6 HUMAN LEUKOCYTARY DIFFERENTIATION ANTIGEN AND ITS USES FOR THE DIAGNOSIS AND TREATMENT OF PSORIASIS
AT407255B (en) * 1997-06-20 2001-02-26 Immuno Ag RECOMBINANT CELL CLONE WITH INCREASED STABILITY IN SERUM- AND PROTEIN-FREE MEDIUM AND METHOD FOR OBTAINING THE STABLE CELL CLONE

Also Published As

Publication number Publication date
TNSN05116A1 (en) 2007-05-14
EP2363418A2 (en) 2011-09-07
LT5354B (en) 2006-07-25
EA200500695A1 (en) 2005-10-27
EP1564285A1 (en) 2005-08-17
CA2503315C (en) 2014-02-18
UY28037A1 (en) 2004-04-30
WO2004038010A1 (en) 2004-05-06
HRP20050760A2 (en) 2006-08-31
KR101186048B1 (en) 2012-09-25
US20060036082A1 (en) 2006-02-16
ES2360049T3 (en) 2011-05-31
US20100197011A1 (en) 2010-08-05
AU2003273727B2 (en) 2008-04-03
BR0315565A (en) 2005-08-23
CN1714147B (en) 2011-01-12
DE60336415D1 (en) 2011-04-28
CU23097A1 (en) 2005-11-18
ES2624279T3 (en) 2017-07-13
CN1714147A (en) 2005-12-28
EA007465B1 (en) 2006-10-27
PT1564285E (en) 2011-04-20
JP2006503879A (en) 2006-02-02
AR041645A1 (en) 2005-05-26
NZ540171A (en) 2008-04-30
PE20040552A1 (en) 2004-10-26
DK1564285T3 (en) 2011-05-16
KR20050088403A (en) 2005-09-06
LT2005053A (en) 2006-04-25
ECSP055740A (en) 2005-09-20
CA2503315A1 (en) 2004-05-06
GEP20084444B (en) 2008-08-10
MY144088A (en) 2011-08-15
BR0315565B1 (en) 2022-01-25
EP2363418B1 (en) 2017-02-08
AU2003273727A1 (en) 2004-05-13
LV13361B (en) 2006-02-20
EP1564285B1 (en) 2011-03-16
HK1084414A1 (en) 2006-07-28
EP2363418A3 (en) 2011-11-16
JP4674087B2 (en) 2011-04-20

Similar Documents

Publication Publication Date Title
DE602005009102D1 (en) Method and system for identifying a culture boundary
DE60228067D1 (en) METHOD FOR DIFFERENTIATING MESENCHYMAL STEM CELLS TO NERVENCELLS
ATE492914T1 (en) METHOD FOR POST-TREATING A PHOTOVOLTAIC CELL
DE60121200D1 (en) METHOD FOR GUIDING A PLANE IN A FORMATION
EP1349918A4 (en) METHOD FOR COLLECTING PLACENTARY STEM CELLS
EP1964045A4 (en) Multi-source bridge network distribution system and method
DE60136755D1 (en) Method for efficient reactive monitoring
DE602005022299D1 (en) METHOD FOR PRODUCING A SOLAR CELL
DE602004016394D1 (en) METHOD OF INSERTING A MARKER IN A CVD DIAMOND
DE602007005355D1 (en) FUEL CELL SYSTEM AND METHOD FOR ESTIMATING THE OUTPUT CHARACTERISTICS OF A FUEL CELL
DE50113124D1 (en) METHOD FOR COMPENSATING TURNING EFFICIENCY IN TURNING SPEED MEASUREMENT
DE602007009856D1 (en) Distribution line and method for producing a distribution line
EP1269266A4 (en) METHOD FOR IMPROVING FOTOMASKEN GEOMETRY
DE602005008121D1 (en) Arrangement and method for searching for a request in digital ink
DE60214168D1 (en) METHOD FOR PRODUCING A HIGH GRADE LINEAR ALCOHOLIC COMPOSITION
DE69904639D1 (en) METHOD FOR EXACTLY DRAWING A CRYSTAL
DE602005003179D1 (en) Method for managing nodes in a group of peer nodes
ATA9462002A (en) METHOD FOR PRODUCING A METAL STRIP WITH A TWO-ROLLING CASTING DEVICE
ATE502103T1 (en) METHOD FOR OBTAINING A RECOMBINANT MAMMAL MYELOMA CELL LINE ADAPTED TO GROWTH IN SERUM-FREE AND PROTEIN-FREE MEDIA
DE50302350D1 (en) METHOD FOR PRODUCING A THROTTLE VALVE IN A THROTTING THROTTLE VALVE SUPPORT
DE60329459D1 (en) Media storage system and method for identifying a storage cell
DE50214759D1 (en) A method for adjusting a signal amplification in a hearing aid and a hearing aid
DE502004009391D1 (en) METHOD FOR SIGNING A QUANTITY OF DATA IN A PUBLIC-KEY SYSTEM AND A DATA PROCESSING SYSTEM FOR CARRYING OUT SAID METHOD
DE60305057D1 (en) SIMPLIFIED METHOD FOR SYMMETRIC KEY RESTORATION IN A DIGITAL NETWORK
DE60017324D1 (en) Method for growing crystals

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1564285

Country of ref document: EP