ATE502122T1 - Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation - Google Patents
Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikationInfo
- Publication number
- ATE502122T1 ATE502122T1 AT07777161T AT07777161T ATE502122T1 AT E502122 T1 ATE502122 T1 AT E502122T1 AT 07777161 T AT07777161 T AT 07777161T AT 07777161 T AT07777161 T AT 07777161T AT E502122 T1 ATE502122 T1 AT E502122T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid amplification
- oligonucleotide primers
- modified oligonucleotide
- chemically modified
- Prior art date
Links
- 239000003155 DNA primer Substances 0.000 title abstract 4
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 125000003636 chemical group Chemical group 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 125000004185 ester group Chemical group 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81066506P | 2006-06-01 | 2006-06-01 | |
| PCT/US2007/011950 WO2007139723A1 (en) | 2006-06-01 | 2007-05-17 | Chemicallymodified oligonucleotide primers for nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE502122T1 true ATE502122T1 (de) | 2011-04-15 |
Family
ID=38778971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07777161T ATE502122T1 (de) | 2006-06-01 | 2007-05-17 | Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US8361753B2 (de) |
| EP (1) | EP2032714B1 (de) |
| JP (1) | JP5558811B2 (de) |
| CN (1) | CN101517091B (de) |
| AT (1) | ATE502122T1 (de) |
| AU (1) | AU2007268075C1 (de) |
| CA (1) | CA2653841C (de) |
| DE (1) | DE602007013223D1 (de) |
| ES (1) | ES2360738T3 (de) |
| WO (1) | WO2007139723A1 (de) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010500011A (ja) | 2006-08-08 | 2010-01-07 | ガンサー ハートマン | ５’リン酸オリゴヌクレオチドの構造および使用 |
| EP2113574A1 (de) * | 2008-04-28 | 2009-11-04 | Biotype AG | Stoffe und Verfahren für Profilierungstest auf DNA-Basis |
| US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP3150727B1 (de) | 2008-04-30 | 2019-07-10 | Integrated DNA Technologies Inc. | Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren |
| US9738680B2 (en) | 2008-05-21 | 2017-08-22 | Rheinische Friedrich-Wilhelms-Universität Bonn | 5′ triphosphate oligonucleotide with blunt end and uses thereof |
| JP5712125B2 (ja) * | 2008-05-27 | 2015-05-07 | トリリンク バイオテクノロジーズ | 高温で開始される核酸増幅のための、化学修飾されたヌクレオシド５’−三リン酸 |
| CN101638685B (zh) * | 2008-07-29 | 2013-01-09 | 杭州优思达生物技术有限公司 | 交叉引物扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用 |
| JP2012514461A (ja) * | 2009-01-06 | 2012-06-28 | ヨウ，キミン | 標的核酸の交差プライミング増幅 |
| EP2488664A4 (de) * | 2009-10-13 | 2014-04-09 | Syntezza Molecular Detection Israel Ltd | Verfahren und zusammensetzungen zur verstärkung von zielsequenzen aus nukleinsäureproben |
| WO2012094343A1 (en) * | 2011-01-05 | 2012-07-12 | Trilink Biotechnologies | Chemically substituted thermosensitive probes and cofactors for hot start ligation |
| KR20140097398A (ko) * | 2011-11-18 | 2014-08-06 | 사렙타 쎄러퓨틱스, 인코퍼레이티드 | 기능적으로-변형된 올리고뉴클레오티드 및 이의 서브유니트 |
| US9353393B2 (en) | 2012-02-15 | 2016-05-31 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| US9150896B2 (en) * | 2012-09-06 | 2015-10-06 | Illumina, Inc. | Nucleotides and primers with removable blocking groups |
| EP2712870A1 (de) | 2012-09-27 | 2014-04-02 | Rheinische Friedrich-Wilhelms-Universität Bonn | Neuartige RIG-I-Liganden und Herstellungsverfahren dafür |
| US20160244812A1 (en) | 2013-10-07 | 2016-08-25 | Mitsui Chemicals, Inc. | Primer set for pcr for bacterial dna amplification, kit for detection and/or identification of bacterial species, and, method of detection and/or identification of bacterial species |
| US9382582B1 (en) * | 2015-08-25 | 2016-07-05 | Tracy Hayden | Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction |
| DK4276189T3 (da) | 2015-09-21 | 2026-05-26 | Trilink Biotechnologies Llc | Initierende oligonukleotidprimere med kappe til syntesering af 5'-ende RNA'er med kappe |
| WO2017214417A1 (en) * | 2016-06-10 | 2017-12-14 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| EP3486321A4 (de) * | 2016-06-23 | 2020-01-15 | Riken | T-zell-rezeptor und b-zell-rezeptor-repertoire-analysesystem unter verwendung einer template-switching-polymerasekettenraktion mit einstufiger reverser transkription |
| KR102757284B1 (ko) | 2017-10-12 | 2025-01-21 | 미쓰이 가가쿠 가부시키가이샤 | mecA 유전자 증폭용 프라이머 페어, mecA 유전자 검출 키트 및 mecA 유전자 검출 방법 |
| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| EP3842550A4 (de) | 2018-08-24 | 2022-06-29 | Repertoire Genesis Incorporation | Verfahren zur analyse eines funktionellen untereinheitspaares eines t-zell-rezeptors und eines b-zell-rezeptors |
| CN109680342B (zh) * | 2018-12-18 | 2021-09-28 | 上海药明康德新药开发有限公司 | DNA编码化合物库中On-DNA芳硝基化合物还原成On-DNA芳胺化合物的方法 |
| WO2020214589A1 (en) * | 2019-04-17 | 2020-10-22 | Sena Research, Incorporated | Modified nucleotides and methods for dna and rna polymerization and sequencing |
| US11739359B2 (en) | 2020-05-01 | 2023-08-29 | Microsoft Technology Licensing, Llc | Universal template strands for enzymatic polynucleotide synthesis |
| CN115916978A (zh) | 2020-05-19 | 2023-04-04 | 哈德森医学研究所 | 寡核苷酸 |
| US11702683B2 (en) | 2020-05-28 | 2023-07-18 | Microsoft Technology Licensing, Llc | De novo polynucleotide synthesis with substrate-bound polymerase |
| CN116368226A (zh) | 2020-09-04 | 2023-06-30 | 维乎医疗有限公司 | 用于加帽rna的组合物和方法 |
| US20250108066A1 (en) | 2022-01-27 | 2025-04-03 | Trilink Biotechnologies, Llc | Trinucleotide cap analogs and methods of use thereof |
| EP4705499A1 (de) | 2023-05-05 | 2026-03-11 | TriLink BioTechnologies, LLC | In vitro transkriptionsverfahren |
| WO2025024563A2 (en) | 2023-07-26 | 2025-01-30 | Trilink Biotechnologies, Llc | Cap analogs and methods of use thereof |
| EP4754109A2 (de) | 2023-08-03 | 2026-06-10 | TriLink BioTechnologies, LLC | Cap-analoga und verfahren zur verwendung davon |
| EP4774281A2 (de) | 2023-09-06 | 2026-07-15 | TriLink BioTechnologies, LLC | Cap-analoga und verfahren zur verwendung davon |
| DE102023208697A1 (de) | 2023-09-08 | 2025-03-13 | Robert Bosch Gesellschaft mit beschränkter Haftung | Oberflächenfunktionalisiertes Substrat und Verfahren zu seiner Herstellung |
| WO2025147660A2 (en) | 2024-01-04 | 2025-07-10 | Trilink Biotechnologies, Llc | Modified rna for increasing protein expression |
| WO2025160336A1 (en) | 2024-01-25 | 2025-07-31 | Trilink Biotechnologies, Llc | Cap analogs and methods of use thereof |
| WO2025184084A1 (en) | 2024-02-26 | 2025-09-04 | Trilink Biotechnologies, Llc | Novel cap analogs and methods of use thereof |
| TW202600819A (zh) | 2024-02-26 | 2026-01-01 | 美商垂林克生物技術有限責任公司 | 新穎帽類似物及其使用方法 |
| WO2025217304A1 (en) * | 2024-04-09 | 2025-10-16 | Bio-Rad Laboratories, Inc. | Compositions and methods for detection of contaminant enzymes |
| WO2026010855A1 (en) | 2024-07-02 | 2026-01-08 | Trilink Biotechnologies, Llc | Modified rna for increasing protein expression |
| WO2026029973A1 (en) | 2024-07-30 | 2026-02-05 | Trilink Biotechnologies, Llc | Cap analogs and methods of use thereof |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5852188A (en) * | 1990-01-11 | 1998-12-22 | Isis Pharmaceuticals, Inc. | Oligonucleotides having chiral phosphorus linkages |
| WO1993014217A1 (en) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules |
| US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US6057134A (en) * | 1996-10-07 | 2000-05-02 | Ambion, Inc. | Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides |
| US6399304B1 (en) * | 1996-12-20 | 2002-06-04 | Roche Diagnostics Gmbh | Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules |
| US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| DK0866071T3 (da) | 1997-03-20 | 2005-01-17 | Hoffmann La Roche | Modificerede primere |
| EP1163251A1 (de) * | 1999-03-24 | 2001-12-19 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | N-acylphosphoramiditen und ihre verwendung in oligonukleotidsynthesen |
| US6274353B1 (en) * | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| AU2001253129A1 (en) * | 2000-04-03 | 2001-10-15 | Biolink Partners, Inc. | Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization |
| WO2002018616A1 (en) | 2000-09-01 | 2002-03-07 | Hitachi Chemical Co., Ltd. | Adjusting the efficiency of nucleic acid template amplification by attenuated pcr with template-mimic oligonucleotides |
| US6743905B2 (en) * | 2001-04-16 | 2004-06-01 | Applera Corporation | Mobility-modified nucleobase polymers and methods of using same |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| EP1275735A1 (de) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
| US7700281B2 (en) | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| GB2416352B (en) | 2004-07-21 | 2009-01-28 | Bioline Ltd | A method for performing the hot start of enzymatic reactions |
| US20070009922A1 (en) * | 2005-01-04 | 2007-01-11 | Stratagene California | Hot start polymerase reaction using a thermolabile blocker |
| EP2405024A1 (de) | 2005-07-15 | 2012-01-11 | Life Technologies Corporation | Heissstart reverse Transkription durch primer design |
-
2007
- 2007-05-17 JP JP2009513171A patent/JP5558811B2/ja active Active
- 2007-05-17 AU AU2007268075A patent/AU2007268075C1/en active Active
- 2007-05-17 US US11/750,237 patent/US8361753B2/en active Active
- 2007-05-17 EP EP07777161A patent/EP2032714B1/de active Active
- 2007-05-17 ES ES07777161T patent/ES2360738T3/es active Active
- 2007-05-17 CN CN200780027958.8A patent/CN101517091B/zh active Active
- 2007-05-17 CA CA2653841A patent/CA2653841C/en active Active
- 2007-05-17 WO PCT/US2007/011950 patent/WO2007139723A1/en not_active Ceased
- 2007-05-17 AT AT07777161T patent/ATE502122T1/de not_active IP Right Cessation
- 2007-05-17 DE DE602007013223T patent/DE602007013223D1/de active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007139723A1 (en) | 2007-12-06 |
| EP2032714A4 (de) | 2009-12-23 |
| EP2032714A1 (de) | 2009-03-11 |
| AU2007268075A1 (en) | 2007-12-06 |
| WO2007139723A8 (en) | 2009-12-17 |
| CA2653841A1 (en) | 2007-12-06 |
| US8361753B2 (en) | 2013-01-29 |
| US20070281308A1 (en) | 2007-12-06 |
| HK1129045A1 (en) | 2009-11-13 |
| ES2360738T3 (es) | 2011-06-08 |
| CA2653841C (en) | 2016-07-19 |
| JP5558811B2 (ja) | 2014-07-23 |
| AU2007268075B2 (en) | 2012-09-13 |
| JP2009538621A (ja) | 2009-11-12 |
| CN101517091A (zh) | 2009-08-26 |
| CN101517091B (zh) | 2013-02-13 |
| EP2032714B1 (de) | 2011-03-16 |
| AU2007268075C1 (en) | 2013-03-07 |
| DE602007013223D1 (de) | 2011-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |