ATE502122T1 - Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation - Google Patents

Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation

Info

Publication number
ATE502122T1
ATE502122T1 AT07777161T AT07777161T ATE502122T1 AT E502122 T1 ATE502122 T1 AT E502122T1 AT 07777161 T AT07777161 T AT 07777161T AT 07777161 T AT07777161 T AT 07777161T AT E502122 T1 ATE502122 T1 AT E502122T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid amplification
oligonucleotide primers
modified oligonucleotide
chemically modified
Prior art date
Application number
AT07777161T
Other languages
English (en)
Inventor
Gerald Zon
Alexandre Lebedev
Original Assignee
Trilink Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trilink Biotechnologies filed Critical Trilink Biotechnologies
Application granted granted Critical
Publication of ATE502122T1 publication Critical patent/ATE502122T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT07777161T 2006-06-01 2007-05-17 Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation ATE502122T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81066506P 2006-06-01 2006-06-01
PCT/US2007/011950 WO2007139723A1 (en) 2006-06-01 2007-05-17 Chemicallymodified oligonucleotide primers for nucleic acid amplification

Publications (1)

Publication Number Publication Date
ATE502122T1 true ATE502122T1 (de) 2011-04-15

Family

ID=38778971

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07777161T ATE502122T1 (de) 2006-06-01 2007-05-17 Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation

Country Status (10)

Country Link
US (1) US8361753B2 (de)
EP (1) EP2032714B1 (de)
JP (1) JP5558811B2 (de)
CN (1) CN101517091B (de)
AT (1) ATE502122T1 (de)
AU (1) AU2007268075C1 (de)
CA (1) CA2653841C (de)
DE (1) DE602007013223D1 (de)
ES (1) ES2360738T3 (de)
WO (1) WO2007139723A1 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2911034T3 (es) 2006-08-08 2022-05-17 Univ Bonn Rheinische Friedrich Wilhelms Estructura y uso de oligonucleótidos 5' fosfato
EP2113574A1 (de) * 2008-04-28 2009-11-04 Biotype AG Stoffe und Verfahren für Profilierungstest auf DNA-Basis
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2644708B1 (de) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren
WO2009141146A1 (en) 2008-05-21 2009-11-26 Gunther Hartmann 5' triphosphate oligonucleotide with blunt end and uses thereof
EP2294076B1 (de) * 2008-05-27 2017-03-08 TriLink BioTechnologies Chemisch modifizierte nukleosid-5'-triphosphate zur wärmeinitiierten nukleinsäure replikation
CN101638685B (zh) * 2008-07-29 2013-01-09 杭州优思达生物技术有限公司 交叉引物扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用
WO2010080691A1 (en) * 2009-01-06 2010-07-15 Qimin You Cross priming amplification of target nucleic acids
CA2777446A1 (en) * 2009-10-13 2011-04-21 Syntezza Molecular Detection Israel Ltd. Methods and compositions for amplifying target sequences from nucleic acid samples
WO2012094343A1 (en) * 2011-01-05 2012-07-12 Trilink Biotechnologies Chemically substituted thermosensitive probes and cofactors for hot start ligation
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
HK1201514A1 (en) * 2011-11-18 2015-09-04 Sarepta Therapeutics, Inc. Functionally-modified oligonucleotides and subunits thereof
US9353393B2 (en) 2012-02-15 2016-05-31 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US9150896B2 (en) * 2012-09-06 2015-10-06 Illumina, Inc. Nucleotides and primers with removable blocking groups
EP2712870A1 (de) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Neuartige RIG-I-Liganden und Herstellungsverfahren dafür
MY195854A (en) 2013-10-07 2023-02-23 Mitsui Chemicals Inc Primer Set for Pcr for Bacterial Dna Amplification, Kit For Detection and/or Identification of Bacterial Species, and, Method of Detection and/or Identification of Bacterial Species
US9382582B1 (en) * 2015-08-25 2016-07-05 Tracy Hayden Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction
CN120842296A (zh) 2015-09-21 2025-10-28 垂林克生物技术有限公司 用于合成5’-加帽rna的组合物和方法
US11371087B2 (en) 2016-06-10 2022-06-28 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
WO2017222056A1 (ja) * 2016-06-23 2017-12-28 国立研究開発法人理化学研究所 ワンステップ逆転写テンプレートスイッチｐｃｒを利用したｔ細胞受容体およびｂ細胞受容体レパトア解析システム
JP7199367B2 (ja) 2017-10-12 2023-01-05 三井化学株式会社 ｍｅｃＡ遺伝子増幅用プライマーペア、ｍｅｃＡ遺伝子検出キット及びｍｅｃＡ遺伝子検出方法
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
TW202031888A (zh) 2018-08-24 2020-09-01 日商組庫創世紀股份有限公司 T細胞受體及b細胞受體之功能性次單元成對基因之解析方法
CN109680342B (zh) * 2018-12-18 2021-09-28 上海药明康德新药开发有限公司 DNA编码化合物库中On-DNA芳硝基化合物还原成On-DNA芳胺化合物的方法
CN114008063A (zh) * 2019-04-17 2022-02-01 硒瑞恩股份有限公司 用于dna和rna聚合和测序的修饰的核苷酸和方法
US11739359B2 (en) 2020-05-01 2023-08-29 Microsoft Technology Licensing, Llc Universal template strands for enzymatic polynucleotide synthesis
KR20230013266A (ko) 2020-05-19 2023-01-26 허드슨 인스티튜트 오브 메디컬 리서치 올리고뉴클레오티드
US11702683B2 (en) 2020-05-28 2023-07-18 Microsoft Technology Licensing, Llc De novo polynucleotide synthesis with substrate-bound polymerase
EP4208549A4 (de) 2020-09-04 2025-04-16 Verve Therapeutics, Inc. Zusammensetzungen und verfahren zum capping von rnas
US20250108066A1 (en) 2022-01-27 2025-04-03 Trilink Biotechnologies, Llc Trinucleotide cap analogs and methods of use thereof
WO2024232914A1 (en) 2023-05-05 2024-11-14 Trilink Biotechnologies, Llc In vitro transcription method
WO2025024563A2 (en) 2023-07-26 2025-01-30 Trilink Biotechnologies, Llc Cap analogs and methods of use thereof
CN121941694A (zh) 2023-08-03 2026-04-28 垂林克生物技术有限公司 帽类似物及其使用方法
WO2025054401A2 (en) 2023-09-06 2025-03-13 Trilink Biotechnologies, Llc Cap analogs and methods of use thereof
DE102023208697A1 (de) 2023-09-08 2025-03-13 Robert Bosch Gesellschaft mit beschränkter Haftung Oberflächenfunktionalisiertes Substrat und Verfahren zu seiner Herstellung
WO2025147660A2 (en) 2024-01-04 2025-07-10 Trilink Biotechnologies, Llc Modified rna for increasing protein expression
WO2025160336A1 (en) 2024-01-25 2025-07-31 Trilink Biotechnologies, Llc Cap analogs and methods of use thereof
TW202600819A (zh) 2024-02-26 2026-01-01 美商垂林克生物技術有限責任公司 新穎帽類似物及其使用方法
WO2025184084A1 (en) 2024-02-26 2025-09-04 Trilink Biotechnologies, Llc Novel cap analogs and methods of use thereof
WO2025217304A1 (en) * 2024-04-09 2025-10-16 Bio-Rad Laboratories, Inc. Compositions and methods for detection of contaminant enzymes
WO2026010855A1 (en) 2024-07-02 2026-01-08 Trilink Biotechnologies, Llc Modified rna for increasing protein expression
WO2026029973A1 (en) 2024-07-30 2026-02-05 Trilink Biotechnologies, Llc Cap analogs and methods of use thereof

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5852188A (en) * 1990-01-11 1998-12-22 Isis Pharmaceuticals, Inc. Oligonucleotides having chiral phosphorus linkages
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6399304B1 (en) * 1996-12-20 2002-06-04 Roche Diagnostics Gmbh Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules
EP0866071B1 (de) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modifizierte Primer
CA2370478A1 (en) * 1999-03-24 2000-09-28 Serge L. Beaucage N-acylphosphoramidites and their use in oligonucleotide synthesis
US6274353B1 (en) * 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
WO2001075139A1 (en) * 2000-04-03 2001-10-11 Biolink Partners, Inc. Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
WO2002018616A1 (en) 2000-09-01 2002-03-07 Hitachi Chemical Co., Ltd. Adjusting the efficiency of nucleic acid template amplification by attenuated pcr with template-mimic oligonucleotides
US6743905B2 (en) * 2001-04-16 2004-06-01 Applera Corporation Mobility-modified nucleobase polymers and methods of using same
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
GB2416352B (en) 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
JP2008526216A (ja) * 2005-01-04 2008-07-24 ストラタジーン カリフォルニア 熱不安定性阻害剤を使用するホットスタートポリメラーゼ反応
US20070128620A1 (en) 2005-07-15 2007-06-07 Applera Corporation Hot start reverse transcription by primer design

Also Published As

Publication number Publication date
WO2007139723A1 (en) 2007-12-06
AU2007268075A1 (en) 2007-12-06
AU2007268075B2 (en) 2012-09-13
JP5558811B2 (ja) 2014-07-23
JP2009538621A (ja) 2009-11-12
WO2007139723A8 (en) 2009-12-17
CN101517091A (zh) 2009-08-26
EP2032714A4 (de) 2009-12-23
US20070281308A1 (en) 2007-12-06
CA2653841A1 (en) 2007-12-06
CN101517091B (zh) 2013-02-13
EP2032714A1 (de) 2009-03-11
DE602007013223D1 (de) 2011-04-28
ES2360738T3 (es) 2011-06-08
AU2007268075C1 (en) 2013-03-07
US8361753B2 (en) 2013-01-29
EP2032714B1 (de) 2011-03-16
CA2653841C (en) 2016-07-19
HK1129045A1 (en) 2009-11-13

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