ATE503018T1 - Transektion filamentöser pilze mit hohem durchsatz - Google Patents
Transektion filamentöser pilze mit hohem durchsatzInfo
- Publication number
- ATE503018T1 ATE503018T1 AT07786832T AT07786832T ATE503018T1 AT E503018 T1 ATE503018 T1 AT E503018T1 AT 07786832 T AT07786832 T AT 07786832T AT 07786832 T AT07786832 T AT 07786832T AT E503018 T1 ATE503018 T1 AT E503018T1
- Authority
- AT
- Austria
- Prior art keywords
- transfection
- cells
- transection
- throughput
- filamentous fungi
- Prior art date
Links
- 241000233866 Fungi Species 0.000 title 1
- 239000000203 mixture Substances 0.000 abstract 3
- 230000002538 fungal effect Effects 0.000 abstract 2
- 238000001890 transfection Methods 0.000 abstract 2
- 239000012096 transfection reagent Substances 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000002207 metabolite Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Spinning Or Twisting Of Yarns (AREA)
- Mushroom Cultivation (AREA)
- Preliminary Treatment Of Fibers (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06116095 | 2006-06-26 | ||
| PCT/EP2007/056311 WO2008000715A1 (en) | 2006-06-26 | 2007-06-25 | High throughput transfection of filamentous fungi |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE503018T1 true ATE503018T1 (de) | 2011-04-15 |
Family
ID=38353831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07786832T ATE503018T1 (de) | 2006-06-26 | 2007-06-25 | Transektion filamentöser pilze mit hohem durchsatz |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8697364B2 (de) |
| EP (1) | EP2032703B1 (de) |
| AT (1) | ATE503018T1 (de) |
| DE (1) | DE602007013396D1 (de) |
| DK (1) | DK2032703T3 (de) |
| WO (1) | WO2008000715A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103596970A (zh) | 2011-03-11 | 2014-02-19 | 帝斯曼知识产权资产管理有限公司 | 载体-宿主系统 |
| JP6821598B2 (ja) | 2015-12-07 | 2021-01-27 | ザイマージェン インコーポレイテッド | Corynebacterium glutamicum由来のプロモーター |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| JP2019519241A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | グルコース透過酵素ライブラリーを生成するための方法およびその使用 |
| EP3478833A4 (de) | 2016-06-30 | 2019-10-02 | Zymergen, Inc. | Verfahren zum erzeugen einer bakteriellen hämoglobin-bibliothek und deren verwendung |
| KR20190098213A (ko) * | 2016-12-30 | 2019-08-21 | 지머젠 인코포레이티드 | 유전자 조작 및 균주 정제를 위한 자동화 단계를 사용하여 균류 생산 균주를 제조하는 방법 |
| KR20200026878A (ko) | 2017-06-06 | 2020-03-11 | 지머젠 인코포레이티드 | 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼 |
| WO2019074737A1 (en) | 2017-10-10 | 2019-04-18 | Dow Agrosciences Llc | NUCLEIC ACID MOLECULES ALDEHYDE DEHYDROGENASE (ALDH1) THAT REGULATE PATHOGENS |
| BR112020024839A2 (pt) | 2018-06-06 | 2021-05-18 | Zymergen Inc. | manipulação de genes envolvidos em transdução de sinal para controlar a morfologia fúngica durante a fermentação e produção |
| EP4055170A1 (de) | 2019-11-04 | 2022-09-14 | DSM IP Assets B.V. | Niedrigvolumentransfektion |
| US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2201730C (en) | 1995-08-03 | 2008-01-22 | Bart Willem Swinkels | The use of homologous amds genes as selectable markers |
| AU4424597A (en) * | 1996-09-13 | 1998-04-02 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
| EP1521837A2 (de) * | 2002-07-05 | 2005-04-13 | Basf Aktiengesellschaft | Plasmidvektoren zur transformation von filamentösen pilzen |
| EP2311942A1 (de) * | 2003-03-21 | 2011-04-20 | Genecor International, Inc. | Neuartige abweichende Hypocrea-jecorina-CBH1-Cellulasen |
| CN1836038B (zh) * | 2003-05-02 | 2011-04-06 | 诺维信股份有限公司 | 制备分泌型多肽的方法 |
| WO2005040186A2 (en) | 2003-10-14 | 2005-05-06 | Dsm Ip Assets B.V. | Method for preparing a modified host cell |
| JP2005269920A (ja) * | 2004-03-23 | 2005-10-06 | Yamaguchi Technology Licensing Organization Ltd | 酵母の形質転換法 |
| US9045748B2 (en) * | 2004-05-27 | 2015-06-02 | Novozymes, Inc. | Methods for transforming and expression screening of filamentous fungal cells with a DNA library |
| CN1981037B (zh) * | 2004-05-27 | 2011-08-31 | 金克克国际有限公司 | 白曲霉酸稳定性α淀粉酶的异源表达和在颗粒淀粉水解中的应用 |
| US7943367B2 (en) * | 2006-09-20 | 2011-05-17 | Bio-Rad Laboratories, Inc. | High-throughput electroporation chamber with functional lid for risk reduction |
-
2007
- 2007-06-25 AT AT07786832T patent/ATE503018T1/de active
- 2007-06-25 US US12/306,314 patent/US8697364B2/en active Active
- 2007-06-25 DE DE602007013396T patent/DE602007013396D1/de active Active
- 2007-06-25 WO PCT/EP2007/056311 patent/WO2008000715A1/en not_active Ceased
- 2007-06-25 DK DK07786832.1T patent/DK2032703T3/da active
- 2007-06-25 EP EP07786832A patent/EP2032703B1/de not_active Revoked
-
2014
- 2014-02-27 US US14/192,236 patent/US9334504B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20140178932A1 (en) | 2014-06-26 |
| DE602007013396D1 (de) | 2011-05-05 |
| WO2008000715A1 (en) | 2008-01-03 |
| US20090280529A1 (en) | 2009-11-12 |
| EP2032703B1 (de) | 2011-03-23 |
| EP2032703A1 (de) | 2009-03-11 |
| US9334504B2 (en) | 2016-05-10 |
| US8697364B2 (en) | 2014-04-15 |
| DK2032703T3 (da) | 2011-06-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE503018T1 (de) | Transektion filamentöser pilze mit hohem durchsatz | |
| Gouw et al. | Quantitative proteomics by metabolic labeling of model organisms | |
| UA116618C2 (uk) | СКОНСТРУЙОВАНЕ НА ОСНОВІ ЦИСТЕЇНУ АНТИТІЛО ПРОТИ CD79b | |
| WO2008064744A8 (en) | A method of preparing polymer dispensed liquid crystal | |
| WO2006102163A3 (en) | Transfection reagents for non-adherent suspension cells | |
| WO2012120500A3 (en) | Low fucose cell lines and uses thereof | |
| MX339645B (es) | Metodos para controlar plagas utilizando arni. | |
| WO2003020931A3 (en) | Sirna knockout assay method and constructs | |
| CA2851316C (en) | Genes and proteins for alkanoyl-coa synthesis | |
| WO2008030845A3 (en) | Methods of predicting distant metastasis of lymph node-negative primary breast cancer using biological pathway gene expression analysis | |
| PT2854552T (pt) | Composição compreendendo um agente de controlo biológico e um fungicida selecionado a partir de inibidores da biossíntese de aminoácidos ou proteínas, inibidores da produção de atp e inibidores da síntese da parede celular | |
| BR112012030646A2 (pt) | cartucho de bebida | |
| MX336473B (es) | Ensayo mediante adcc nk facs tridimensional. | |
| WO2005042692A3 (en) | A method of inhibiting the expression of genes which mediate cellular cholesterol influx in animal cells and inhibiting the production of proteins resulting from the expression of such genes using cholesterol absorption inhibitors | |
| WO2011008785A3 (en) | A method of analyzing cellulose decay in cellulosic material hydrolysis | |
| WO2009158627A3 (en) | Cellulosic protein expression in yeast | |
| WO2013048899A3 (en) | Methods for the on-site production of chloramine and its use thereof | |
| EP2204194A3 (de) | Haarzellensonde und Markierverfahren für Haarzellen mit der Sonde für Haarzellen | |
| MX2013012264A (es) | Dispensador con elemento de ruptura. | |
| WO2010032008A3 (en) | Dendritic cell modulatory molecule | |
| ATE463559T1 (de) | Zelllinie zur produktion von coronaviren | |
| WO2006053251A3 (en) | Method of measuring amyloid-beta peptides | |
| WO2008090148A3 (en) | Improvement of cell growth | |
| WO2010002620A8 (en) | Modifying contents within liquid containers | |
| EA201070152A1 (ru) | Способ получения стабильных клеточных линий, экспрессирующих высокие уровни представляющего интерес белка |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 2032703 Country of ref document: EP |