ATE504603T1 - METHOD FOR PRODUCING INTERESTING POLYPEPTIDES FROM FUSION POLYPEPTIDES - Google Patents

METHOD FOR PRODUCING INTERESTING POLYPEPTIDES FROM FUSION POLYPEPTIDES

Info

Publication number
ATE504603T1
ATE504603T1 AT02753269T AT02753269T ATE504603T1 AT E504603 T1 ATE504603 T1 AT E504603T1 AT 02753269 T AT02753269 T AT 02753269T AT 02753269 T AT02753269 T AT 02753269T AT E504603 T1 ATE504603 T1 AT E504603T1
Authority
AT
Austria
Prior art keywords
polypeptides
fusion
protein
target
producing interesting
Prior art date
Application number
AT02753269T
Other languages
German (de)
Inventor
Woo-Jong Lee
Heung-Bok Park
Tae-Hoon Cho
Jeong-Min Kim
Yeon-Sung Park
Original Assignee
Advanced Protein Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Protein Technologies Corp filed Critical Advanced Protein Technologies Corp
Priority claimed from PCT/KR2002/001416 external-priority patent/WO2003010204A1/en
Application granted granted Critical
Publication of ATE504603T1 publication Critical patent/ATE504603T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE: Provided is a simple and economical process for separating target polypeptides from fusion polypeptides in high purity and yield, thereby solving problems in purification process required for the production of recombination proteins. CONSTITUTION: The process for separating target polypeptides from fusion polypeptides is characterized by expressing a fusion protein of a target protein and a fusion partner; loading the fusion protein on a matrix; treating the fusion protein with ubiquitinase; and recovering the target protein.
AT02753269T 2001-07-26 2002-07-26 METHOD FOR PRODUCING INTERESTING POLYPEPTIDES FROM FUSION POLYPEPTIDES ATE504603T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20010045229 2001-07-26
KR10-2002-0043968A KR100535265B1 (en) 2001-07-26 2002-07-25 Process for preparation of polypeptides of interest from fusion polypeptides
PCT/KR2002/001416 WO2003010204A1 (en) 2001-07-26 2002-07-26 Process for preparation of polypeptides of interest from fusion peolypeptides

Publications (1)

Publication Number Publication Date
ATE504603T1 true ATE504603T1 (en) 2011-04-15

Family

ID=27716835

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02753269T ATE504603T1 (en) 2001-07-26 2002-07-26 METHOD FOR PRODUCING INTERESTING POLYPEPTIDES FROM FUSION POLYPEPTIDES

Country Status (3)

Country Link
KR (1) KR100535265B1 (en)
AT (1) ATE504603T1 (en)
DE (1) DE60239684D1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101367869B1 (en) * 2011-12-29 2014-02-28 주식회사 삼양바이오팜 Purification method of peptidylglycine alpha amidating monooxygenase (pam) in high purity by lysine tagging at c-terminal of pam
KR101906319B1 (en) 2017-12-04 2018-10-10 주식회사 삼양바이오팜 Process for purification of recombinant adenovirus type 3 fiber polypeptide

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196321A (en) * 1986-10-02 1993-03-23 Massachusetts Institute Of Technology Methods for in vitro cleavage of ubiquitin fusion proteins
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
US6068994A (en) * 1989-08-07 2000-05-30 Chiron Corporation Ubiquitin expression system
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins

Also Published As

Publication number Publication date
KR100535265B1 (en) 2005-12-08
DE60239684D1 (en) 2011-05-19
KR20030010536A (en) 2003-02-05

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Legal Events

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