ATE510909T1 - Restriktions-endonukleasen mit hoher wiedergabetreue - Google Patents

Restriktions-endonukleasen mit hoher wiedergabetreue

Info

Publication number
ATE510909T1
ATE510909T1 AT08796186T AT08796186T ATE510909T1 AT E510909 T1 ATE510909 T1 AT E510909T1 AT 08796186 T AT08796186 T AT 08796186T AT 08796186 T AT08796186 T AT 08796186T AT E510909 T1 ATE510909 T1 AT E510909T1
Authority
AT
Austria
Prior art keywords
restriction endonuclease
mutation
artificially introduced
introduced mutation
high fidelity
Prior art date
Application number
AT08796186T
Other languages
English (en)
Inventor
Zhenyu Zhu
Aine Blanchard
Shuang-Yong Xu
Shengxi Guan
Hua Wei
Penghua Zhang
Dapeng Sun
Siu-Hong Chan
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of ATE510909T1 publication Critical patent/ATE510909T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT08796186T 2007-07-12 2008-07-14 Restriktions-endonukleasen mit hoher wiedergabetreue ATE510909T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95920307P 2007-07-12 2007-07-12
PCT/US2008/069997 WO2009009797A2 (en) 2007-07-12 2008-07-14 High fidelity restriction endonucleases

Publications (1)

Publication Number Publication Date
ATE510909T1 true ATE510909T1 (de) 2011-06-15

Family

ID=39832374

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08796186T ATE510909T1 (de) 2007-07-12 2008-07-14 Restriktions-endonukleasen mit hoher wiedergabetreue

Country Status (6)

Country Link
US (15) US8372619B2 (de)
EP (20) EP2392651B1 (de)
JP (4) JP5988471B2 (de)
CN (9) CN101802183B (de)
AT (1) ATE510909T1 (de)
WO (1) WO2009009797A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101802183B (zh) * 2007-07-12 2016-02-24 新英格兰生物实验室公司 高保真度限制性内切核酸酶
DE112011100467B4 (de) 2010-02-05 2018-07-12 New England Biolabs, Inc. Restriktionsendonukleasen mit hoher Genauigkeit
US10287620B2 (en) * 2016-04-29 2019-05-14 Bio-Rad Laboratories, Inc. Digital polymerase fidelity assay
US10947518B1 (en) * 2019-10-18 2021-03-16 Abclonal Science, Inc. Using proteases to control restriction enzyme activity
CN112662647A (zh) * 2021-01-08 2021-04-16 上海咏科生物科技有限公司 重组NcoI限制性内切酶的制备方法
CN113832127A (zh) * 2021-10-27 2021-12-24 生工生物工程（上海）股份有限公司 一种限制性内切酶BamHⅠ的突变体及其应用
CN116083544B (zh) * 2022-03-18 2024-11-08 华中科技大学 一种m6A依赖型限制性内切酶、其突变体及其应用
WO2023209649A1 (en) * 2022-04-29 2023-11-02 Seqirus, Inc. Method of dna linearization
CN117778498A (zh) * 2022-09-26 2024-03-29 深圳瑞吉生物科技有限公司 一种无细胞制备mRNA转录模板的方法
US12306823B2 (en) * 2023-01-23 2025-05-20 Dell Products L.P. Data storage system ensuring consistency of data received by a producer from a collector via a shared memory
US20250122489A1 (en) * 2023-10-16 2025-04-17 Zhenyu Zhu Protein Thermostability Reduction by Tag Fusion and Its Usage

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1594399A (de) 1968-12-12 1970-06-01
US4935367A (en) * 1988-03-17 1990-06-19 The United States Of America As Represented By The Department Of Health And Human Services Novel restriction endonuclease
DE3811277A1 (de) * 1988-04-02 1989-10-12 Boehringer Mannheim Gmbh Typ ii-restriktionsendonuklease eclxi
DE4009663A1 (de) * 1990-03-26 1991-10-02 Boehringer Mannheim Gmbh Verfahren zur verminderung von sternaktivitaeten
US5202248A (en) * 1990-11-02 1993-04-13 New England Biolabs, Inc. Method for cloning and producing the nco i restriction endonuclease and methylase
US5262318A (en) * 1992-08-20 1993-11-16 New England Biolabs, Inc. Isolated DNA encoding the SPHI restriction endonuclease and related methods for producing the same
US5371006A (en) * 1993-01-11 1994-12-06 New England Biolabs, Inc. Isolated DNA encoding the NotI restriction endonuclease and related methods for producing the same
US5516678A (en) * 1994-10-06 1996-05-14 New England Biolabs, Inc. Method for producing the SSPI restriction endonuclease and methylase
US5532153A (en) * 1995-03-23 1996-07-02 New England Biolabs, Inc. Method for cloning and producing the SacI restriction endonuclease
US5721126A (en) * 1995-12-08 1998-02-24 New England Biolabs, Inc. Method for cloning and producing the SCaI restriction endonuclease in E. coli
US5663067A (en) * 1996-07-11 1997-09-02 New England Biolabs, Inc. Method for cloning and producing the SapI restriction endonuclease in E. coli
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6849428B1 (en) 1997-03-05 2005-02-01 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
US5885818A (en) * 1998-05-14 1999-03-23 New England Biolabs, Inc. Method for cloning and producing ageI restriction endonuclease in E. coli
US6387681B1 (en) * 1999-10-28 2002-05-14 New England Biolabs, Inc. Method for cloning and expression of NHEI restriction endonuclease in E. coli.
JP2003259876A (ja) * 2002-03-08 2003-09-16 Univ Waseda 変異制限酵素
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
WO2004007670A2 (en) * 2002-07-12 2004-01-22 New England Biolabs, Inc. RECOMBINANT TYPE II RESTRICTION ENDONUCLEASES, MmeI AND RELATED ENDONUCLEASES AND METHODS FOR PRODUCTING THE SAME
US20040175816A1 (en) * 2003-03-03 2004-09-09 New England Biolabs, Inc. Method for cloning and expression of OkrAI restriction endonuclease and methyltransferase
US20050003420A1 (en) * 2003-07-01 2005-01-06 Xu Shuang-Yong Recycled mutagenesis of restriction endonuclease toward enhanced catalytic activity
US6958230B2 (en) * 2003-09-22 2005-10-25 New England Biolabs, Inc. Method for cloning and expression of SbfI restriction endonuclease and SbfI methylase in E. coli
US7943303B2 (en) * 2003-12-18 2011-05-17 New England Biolabs, Inc. Method for engineering strand-specific nicking endonucleases from restriction endonucleases
CA2572917C (en) * 2004-07-06 2012-04-03 Bioren Inc. Look-through mutagenesis for developing altered polypeptides with enhanced properties
WO2006012593A1 (en) * 2004-07-22 2006-02-02 Shuang-Yong Xu Nicking endonuclease methods and compositions
EP2568040A1 (de) * 2005-08-04 2013-03-13 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
ATE493493T1 (de) * 2005-08-31 2011-01-15 New England Biolabs Inc Kpni-restriktionsendonukleasen mit reduzierter star-aktivität
CN101802183B (zh) * 2007-07-12 2016-02-24 新英格兰生物实验室公司 高保真度限制性内切核酸酶
CN103088015A (zh) * 2013-01-16 2013-05-08 上海中医药大学 一种dna多位点定向突变方法
CN104562214A (zh) * 2014-12-26 2015-04-29 上海派森诺生物科技有限公司 一种基于ⅱb型限制性内切酶酶切的简化基因组建库方法

Also Published As

Publication number Publication date
EP2463364B1 (de) 2013-09-04
US20210230581A1 (en) 2021-07-29
CN105441409A (zh) 2016-03-30
EP2458001A1 (de) 2012-05-30
CN113186177A (zh) 2021-07-30
EP2392653B1 (de) 2014-03-19
US20130115678A1 (en) 2013-05-09
US10450559B2 (en) 2019-10-22
EP2395079B1 (de) 2015-01-07
US9074196B2 (en) 2015-07-07
US20160102306A1 (en) 2016-04-14
CN105647886A (zh) 2016-06-08
US20130115680A1 (en) 2013-05-09
EP2390320B1 (de) 2015-01-21
US20240352447A1 (en) 2024-10-24
US9074197B2 (en) 2015-07-07
CN105441408B (zh) 2025-01-24
US8691549B2 (en) 2014-04-08
US20130115679A1 (en) 2013-05-09
EP2167655B1 (de) 2011-05-25
EP2455464A1 (de) 2012-05-23
CN101802183B (zh) 2016-02-24
US20130122568A1 (en) 2013-05-16
US9034625B2 (en) 2015-05-19
US20180171328A1 (en) 2018-06-21
CN119060985A (zh) 2024-12-03
EP2390320A1 (de) 2011-11-30
US8673610B2 (en) 2014-03-18
CN103361329B (zh) 2020-03-20
EP2455464B1 (de) 2013-09-04
JP2014027938A (ja) 2014-02-13
CN105441408A (zh) 2016-03-30
JP2016154545A (ja) 2016-09-01
WO2009009797A2 (en) 2009-01-15
EP2458001B1 (de) 2013-09-04
EP2392654A1 (de) 2011-12-07
EP2390315A1 (de) 2011-11-30
EP2392651B1 (de) 2014-08-20
US9249396B2 (en) 2016-02-02
EP2390319B1 (de) 2014-12-24
EP2390318B1 (de) 2014-07-02
JP2010532999A (ja) 2010-10-21
EP2390316B1 (de) 2014-03-19
CN105441407B (zh) 2025-02-21
US20130115677A1 (en) 2013-05-09
EP2390314A1 (de) 2011-11-30
US20130115681A1 (en) 2013-05-09
EP2167655A2 (de) 2010-03-31
EP2390318A1 (de) 2011-11-30
US12516316B2 (en) 2026-01-06
EP2455463A3 (de) 2012-09-05
EP2392651A1 (de) 2011-12-07
EP2455463A2 (de) 2012-05-23
US20130130935A1 (en) 2013-05-23
EP2463363A1 (de) 2012-06-13
EP2390315B1 (de) 2014-11-19
CN105483097A (zh) 2016-04-13
EP2463363B1 (de) 2013-09-04
US8372619B2 (en) 2013-02-12
EP2390319A1 (de) 2011-11-30
JP2018007675A (ja) 2018-01-18
JP5988471B2 (ja) 2016-09-07
US9023634B2 (en) 2015-05-05
US8628945B2 (en) 2014-01-14
JP6684250B2 (ja) 2020-04-22
CN105441407A (zh) 2016-03-30
EP2468854B1 (de) 2013-09-11
EP2468854A1 (de) 2012-06-27
EP2392650A1 (de) 2011-12-07
JP6012562B2 (ja) 2016-10-25
EP2392653A1 (de) 2011-12-07
US9193960B2 (en) 2015-11-24
EP2390315A8 (de) 2012-05-09
EP2395079A1 (de) 2011-12-14
EP2463364A1 (de) 2012-06-13
CN105483097B (zh) 2021-07-13
US20130190209A1 (en) 2013-07-25
EP2390317A1 (de) 2011-11-30
US12054707B2 (en) 2024-08-06
EP2390316A1 (de) 2011-11-30
CN101802183A (zh) 2010-08-11
WO2009009797A3 (en) 2009-03-12
EP2390314B1 (de) 2014-03-19
CN113186177B (zh) 2024-09-20
US20200063121A1 (en) 2020-02-27
EP2390317B1 (de) 2014-06-25
EP2392652B1 (de) 2014-03-19
US20090029376A1 (en) 2009-01-29
CN103361329A (zh) 2013-10-23
US20130115676A1 (en) 2013-05-09
CN105441409B (zh) 2020-11-06
EP2392650B1 (de) 2014-03-19
EP2392654B1 (de) 2015-01-21
EP2392652A1 (de) 2011-12-07

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