ATE513034T1 - Verfahren zur gewinnung immortalisierter antikörpersezernierender zellen - Google Patents

Verfahren zur gewinnung immortalisierter antikörpersezernierender zellen

Info

Publication number
ATE513034T1
ATE513034T1 AT06830666T AT06830666T ATE513034T1 AT E513034 T1 ATE513034 T1 AT E513034T1 AT 06830666 T AT06830666 T AT 06830666T AT 06830666 T AT06830666 T AT 06830666T AT E513034 T1 ATE513034 T1 AT E513034T1
Authority
AT
Austria
Prior art keywords
cells
methods
human
secrete
antibodies
Prior art date
Application number
AT06830666T
Other languages
English (en)
Inventor
Ada Funaro
Gianni Garotta
Marianne Murphy
Original Assignee
Ribovax Biotechnologies Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribovax Biotechnologies Sa filed Critical Ribovax Biotechnologies Sa
Application granted granted Critical
Publication of ATE513034T1 publication Critical patent/ATE513034T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT06830666T 2005-12-16 2006-12-15 Verfahren zur gewinnung immortalisierter antikörpersezernierender zellen ATE513034T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP2005056871 2005-12-16
PCT/EP2006/069780 WO2007068758A1 (en) 2005-12-16 2006-12-15 Methods for obtaining immortalized antibody secreting cells

Publications (1)

Publication Number Publication Date
ATE513034T1 true ATE513034T1 (de) 2011-07-15

Family

ID=36685876

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06830666T ATE513034T1 (de) 2005-12-16 2006-12-15 Verfahren zur gewinnung immortalisierter antikörpersezernierender zellen

Country Status (19)

Country Link
US (1) US8338172B2 (de)
EP (1) EP1974020B1 (de)
JP (2) JP5431730B2 (de)
KR (1) KR101508945B1 (de)
CN (1) CN101374944B (de)
AT (1) ATE513034T1 (de)
AU (1) AU2006325236B2 (de)
BR (1) BRPI0619908A2 (de)
CA (1) CA2633601C (de)
DK (1) DK1974020T3 (de)
EA (1) EA019505B1 (de)
ES (1) ES2367322T3 (de)
IL (1) IL192095A0 (de)
NO (1) NO20083160L (de)
NZ (1) NZ569816A (de)
PL (1) PL1974020T3 (de)
PT (1) PT1974020E (de)
WO (1) WO2007068758A1 (de)
ZA (2) ZA200806094B (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2398783A (en) 2003-02-26 2004-09-01 Antonio Lanzavecchia A method for producing immortalised human B memory lymphocytes
GB0700133D0 (en) 2007-01-04 2007-02-14 Humabs Llc Human cytomegalovirus neutralising antibodies and use thereof
US7947274B2 (en) 2007-01-04 2011-05-24 Humabs, LLC. Human cytomegalovirus neutralising antibodies and use thereof
WO2009003975A1 (en) * 2007-07-04 2009-01-08 Ribovax Biotechnologies Sa Antibodies against human cytomegalovirus (hcmv)
AU2008284015B2 (en) 2007-08-03 2014-05-15 Musc Foundation For Research Development Human monoclonal antibodies and methods for producing the same
WO2009024445A1 (en) * 2007-08-22 2009-02-26 Ribovax Biotechnologies Sa Antibodies against human cytomegalovirus (hcmv)
CA2730776C (en) 2008-07-16 2018-12-11 Institute For Research In Biomedicine Human cytomegalovirus neutralizing antibodies and use thereof
WO2010034103A1 (en) 2008-09-23 2010-04-01 Héma-Québec Method for polyclonal immunoglobulin g production by human b cells
US8568719B2 (en) 2009-08-13 2013-10-29 Crucell Holland B.V. Antibodies against human respiratory syncytial virus (RSV) and methods of use
EP2501800A4 (de) 2009-11-17 2013-05-22 Musc Found For Res Dev Humane monoklonale antikörper gegen menschliches nukleolin
WO2011076883A1 (en) 2009-12-23 2011-06-30 4-Antibody Ag Binding members for human cytomegalovirus
SI2400298T1 (sl) * 2010-05-28 2013-11-29 F. Hoffmann-La Roche Ag Postopek kultivacije posamezne B-celice in izdelava specifičnih protiteles
JP5744196B2 (ja) 2010-07-09 2015-07-08 クルセル ホランド ベー ヴェー 抗ヒト呼吸器多核体ウイルス（ｒｓｖ）抗体および使用の方法
CN102839152A (zh) * 2011-06-20 2012-12-26 中国科学院上海生命科学研究院 一种快速高效获得抗原特异性b细胞的方法
US20140287402A1 (en) * 2011-06-27 2014-09-25 Valneva Method for screening cells
US9757458B2 (en) * 2011-12-05 2017-09-12 Immunomedics, Inc. Crosslinking of CD22 by epratuzumab triggers BCR signaling and caspase-dependent apoptosis in hematopoietic cancer cells
US10131709B2 (en) 2011-12-28 2018-11-20 Immunoqure Ag Nucleic acid molecules encoding monoclonal antibodies specific for IL-22
HUE037186T2 (hu) 2011-12-28 2018-08-28 Immunoqure Ag Eljárás humán antitestek izolálására
US20140199319A1 (en) * 2012-12-14 2014-07-17 Abbvie, Inc. Methods for increasing the efficiency of hybridoma generation
JP6421944B2 (ja) * 2013-01-03 2018-11-14 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ サッカロミセス・セレビシエでの発現によって分泌可能な抗体を製造する方法
WO2015001047A1 (en) 2013-07-03 2015-01-08 Immunoqure Ag Method of providing anti-human cytokine antibodies for pharmaceutical use
US10611800B2 (en) 2016-03-11 2020-04-07 Pfizer Inc. Human cytomegalovirus gB polypeptide
WO2018122147A1 (en) * 2017-01-02 2018-07-05 F. Hoffmann-La Roche Ag B-cell cultivation method
CA3050914A1 (en) 2017-01-27 2018-08-02 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Vaccine compositions of herpesvirus envelope protein combinations to induce immune response
US11629172B2 (en) 2018-12-21 2023-04-18 Pfizer Inc. Human cytomegalovirus gB polypeptide
CN111647566A (zh) * 2019-07-10 2020-09-11 广州医科大学附属第一医院 一种高滴度eb病毒的制备方法、eb病毒永生化记忆性b细胞的方法与应用
TWI810589B (zh) 2020-06-21 2023-08-01 美商輝瑞股份有限公司 人巨細胞病毒糖蛋白Ｂ（ｇＢ）多肽
WO2022120334A1 (en) 2020-12-03 2022-06-09 Century Therapeutics, Inc. Genetically engineered cells and uses thereof
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
AU2021401052A1 (en) 2020-12-18 2023-06-22 Century Therapeutics, Inc. Chimeric antigen receptor systems with adaptable receptor specificity
KR20250089553A (ko) * 2022-12-06 2025-06-18 기타야마 라베스 가부시키가이샤 불멸화 세포의 제조 방법
CN117230258B (zh) * 2023-11-10 2024-04-09 苏州驾玉生物医药有限公司 一种提高灵敏度的培养扩增联合pcr的eb病毒检测方法
CN119285758B (zh) * 2024-10-31 2025-07-01 上海百英生物科技股份有限公司 一种基于b细胞永生化的单个b细胞抗体开发方法及其应用
CN120272432B (zh) * 2025-03-25 2025-12-26 佛山市妇幼保健院 一种脊髓性肌萎缩症质控品及其制备方法和应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4997764A (en) * 1987-04-23 1991-03-05 New York University Transformation of human B-lympocytes with Epstein Barr virus and c-myc containing vectors
EP0493433B1 (de) 1989-09-19 1996-01-10 Centocor, Inc. Verfahren zur verbesserten herstellung von menschlichen antikörpern sowie hierzu benutzte zellen
ATE449853T1 (de) * 1990-02-01 2009-12-15 Siemens Healthcare Diagnostics HERSTELLUNG UND VERWENDUNG VON GENBANKEN MENSCHLICHER ANTIKÖRPER(ßHUMAN-ANTIKÖRPER- BIBLIOTHEKENß)
US5811524A (en) * 1995-06-07 1998-09-22 Idec Pharmaceuticals Corporation Neutralizing high affinity human monoclonal antibodies specific to RSV F-protein and methods for their manufacture and therapeutic use thereof
US6399384B1 (en) * 1999-09-17 2002-06-04 Reneuron Limited Conditional immortalization of cells
FR2817875B1 (fr) * 2000-12-07 2005-03-18 Technopharm Procede de preparation d'un anticorps monoclonal humain, de fragments de celui-ci ou d'anticorps comprenant de tels fragments, les anticorps ainsi obtenus et leur utilisation
GB2398783A (en) * 2003-02-26 2004-09-01 Antonio Lanzavecchia A method for producing immortalised human B memory lymphocytes
EP1597280B2 (de) * 2003-02-26 2016-08-24 Institute for Research in Biomedicine Monoklonale antikörperproduktion durch ebv transformation von b-zellen
WO2007049152A2 (en) * 2005-08-23 2007-05-03 Iq Corporation Methods of producing stable b-lymphocytes

Also Published As

Publication number Publication date
DK1974020T3 (da) 2011-10-03
ES2367322T3 (es) 2011-11-02
ZA200806094B (en) 2009-06-24
WO2007068758A1 (en) 2007-06-21
US8338172B2 (en) 2012-12-25
BRPI0619908A2 (pt) 2011-10-25
JP5431730B2 (ja) 2014-03-05
EA200870062A1 (ru) 2009-04-28
PL1974020T3 (pl) 2011-11-30
NO20083160L (no) 2008-09-10
CA2633601C (en) 2015-06-30
NZ569816A (en) 2011-10-28
EP1974020A1 (de) 2008-10-01
CA2633601A1 (en) 2007-06-21
EA019505B1 (ru) 2014-04-30
ZA200904907B (en) 2010-04-28
AU2006325236A1 (en) 2007-06-21
JP2013255515A (ja) 2013-12-26
HK1127783A1 (en) 2009-10-09
CN101374944A (zh) 2009-02-25
KR101508945B1 (ko) 2015-04-08
KR20080097181A (ko) 2008-11-04
CN101374944B (zh) 2012-02-08
JP2009519026A (ja) 2009-05-14
EP1974020B1 (de) 2011-06-15
AU2006325236B2 (en) 2011-11-10
US20090270268A1 (en) 2009-10-29
IL192095A0 (en) 2008-12-29
PT1974020E (pt) 2011-08-26

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