ATE513038T1 - Produktion eines transgenen vogels mittels cytoplasmainjektion - Google Patents

Produktion eines transgenen vogels mittels cytoplasmainjektion

Info

Publication number
ATE513038T1
ATE513038T1 AT02775895T AT02775895T ATE513038T1 AT E513038 T1 ATE513038 T1 AT E513038T1 AT 02775895 T AT02775895 T AT 02775895T AT 02775895 T AT02775895 T AT 02775895T AT E513038 T1 ATE513038 T1 AT E513038T1
Authority
AT
Austria
Prior art keywords
cytoplasm
heterologous protein
bird
transgenic
methods
Prior art date
Application number
AT02775895T
Other languages
English (en)
Inventor
Jeffrey Rapp
Leandro Christmann
Original Assignee
Synageva Biopharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synageva Biopharma Corp filed Critical Synageva Biopharma Corp
Application granted granted Critical
Publication of ATE513038T1 publication Critical patent/ATE513038T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/23—Immunoglobulins specific features characterized by taxonomic origin from birds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/90—Vector systems having a special element relevant for transcription from vertebrates avian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
AT02775895T 2001-09-18 2002-09-18 Produktion eines transgenen vogels mittels cytoplasmainjektion ATE513038T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32296901P 2001-09-18 2001-09-18
US35155002P 2002-01-25 2002-01-25
PCT/US2002/029878 WO2003025146A2 (en) 2001-09-18 2002-09-18 Production of a transgenic avian by cytoplasmic injection

Publications (1)

Publication Number Publication Date
ATE513038T1 true ATE513038T1 (de) 2011-07-15

Family

ID=26983697

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02775895T ATE513038T1 (de) 2001-09-18 2002-09-18 Produktion eines transgenen vogels mittels cytoplasmainjektion

Country Status (5)

Country Link
US (1) US7312374B2 (de)
EP (1) EP1438401B1 (de)
AT (1) ATE513038T1 (de)
AU (1) AU2002341746A1 (de)
WO (1) WO2003025146A2 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001520009A (ja) * 1997-10-16 2001-10-30 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド 鳥の導入遺伝子についてのマグナム特異的プロモーターを含むベクター
US20040019923A1 (en) * 1997-10-16 2004-01-29 Ivarie Robert D. Exogenous proteins expressed in avians and their eggs
US20020116732A1 (en) * 2001-02-13 2002-08-22 Leandro Christmann Microinjection assembly and methods for microinjecting and reimplanting avian eggs
US20080124787A1 (en) * 2001-02-13 2008-05-29 Avigenics, Inc. Microinjection devices and methods of use
US7339090B2 (en) * 2001-02-13 2008-03-04 Avigenics, Inc. Microinjection devices and methods of use
US7541512B2 (en) * 2001-03-30 2009-06-02 Synageva Biopharma Corp. Avians containing a lysozyme promoter transgene
US7294507B2 (en) * 2001-11-30 2007-11-13 Avigenics, Inc. Ovomucoid promoters and methods of use
US7335761B2 (en) * 2001-11-30 2008-02-26 Avigenics, Inc. Avian gene expression controlling regions
US20100333219A1 (en) * 2001-11-30 2010-12-30 Synageva Biopharma Corp. Methods of protein production using ovomucoid regulatory regions
US7323618B2 (en) * 2002-02-01 2008-01-29 Origen Therapeutics, Inc. Tissue specific expression of exogenous proteins in transgenic chickens
US20060191026A1 (en) 2005-02-18 2006-08-24 Origen Therapeutics, Inc. Tissue specific expression of antibodies in chickens
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
BR0313441A (pt) * 2002-08-13 2005-07-12 Kaneka Corp Método para expressar gene em aves transgênicas usando vetor retroviral, e as aves transgênicas assim obtidas
US7803362B2 (en) 2003-01-24 2010-09-28 Synageva Biopharma Corp. Glycosylated interferon alpha
US20050198700A1 (en) * 2003-03-07 2005-09-08 Avigenics, Inc. Genomic modification
US20050034186A1 (en) * 2003-03-07 2005-02-10 Harvey Alex J. Site specific nucleic acid integration
US20040255345A1 (en) * 2003-03-07 2004-12-16 Rapp Jeffrey C. Production of transgenic avians
US20050273873A1 (en) * 2003-03-07 2005-12-08 Avigenics, Inc. Genomic modification
WO2004080162A2 (en) * 2003-03-07 2004-09-23 Avigenics, Inc. Integrase mediated avian transgenesis
EP1623035A4 (de) * 2003-04-15 2006-08-30 Univ Georgia Res Found Vogeltransgenese unter verwendung eines hühnereialbumin-genbereichs
US7381712B2 (en) * 2003-05-09 2008-06-03 Avigenics, Inc. In vivo transfection in avians
US20080222744A1 (en) * 2003-05-09 2008-09-11 Avigenics, Inc. In vivo transfection in avians
KR100569163B1 (ko) * 2003-08-11 2006-04-07 (주)아비코아생명공학연구소 정원세포를 이용한 조류 카이메라의 생산방법 및 조류 카이메라
US20050090001A1 (en) 2003-10-07 2005-04-28 Parker Stephen H. Cell lines and methods for producing proteins
US8071364B2 (en) * 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
EP1708564A4 (de) * 2004-01-15 2007-03-07 Avigenics Inc Produktion eines transgenen vogels mittels cytoplasmainjektion
US20060174364A1 (en) * 2004-03-01 2006-08-03 Avigenics, Inc. Artificial chromosomes and transchromosomic avians
US20060123504A1 (en) * 2004-12-07 2006-06-08 Avigenics, Inc. Methods of producing polyclonal antibodies
US20070190601A1 (en) * 2004-10-08 2007-08-16 Avigenics, Inc. Modified integrase and methods of use
US7812127B2 (en) 2006-03-17 2010-10-12 Synageva Biopharma Corp. Glycosylated human G-CSF
US20090105462A1 (en) * 2006-11-09 2009-04-23 Ivarie Robert D Glycosylated erythropoietin
KR20090090328A (ko) * 2006-11-09 2009-08-25 시나게바 바이오파르마, 코포레이션 조류 유래 에리트로포이어틴
US20100081789A1 (en) * 2008-09-25 2010-04-01 Cooper Richard K Novel Vectors for Production of Interferon
WO2010036978A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
WO2010036976A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
US9150881B2 (en) * 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors
EP2435476A4 (de) * 2009-05-27 2013-04-17 Synageva Biopharma Corp Aus vögeln gewonnene antikörper
WO2011060242A2 (en) 2009-11-13 2011-05-19 Talecris Biotherapeutics, Inc. Von willebrand factor (vwf)-containing preparations, and methods, kits, and uses related thereto
GB201420139D0 (en) 2014-11-12 2014-12-24 Ucl Business Plc Factor IX gene therapy
US10858669B2 (en) * 2015-12-25 2020-12-08 National Institute Of Advanced Industrial Science And Technology Genetically modified chicken egg with an exogenous sequence knocked into the ovalbumin gene
JP2020536580A (ja) * 2017-09-19 2020-12-17 ザ ステート オブ イスラエル， ミニストリー オブ アグリカルチャー アンド ルーラル ディヴェロプメント， アグリカルチュラル リサーチ オーガニゼーション （エーアールオー） （ボルカニ センター） ゲノム編集された鳥
US12134790B2 (en) 2017-09-27 2024-11-05 Inozyme Pharma, Inc. Methods of improving cardiovascular function and treating cardiovascular disease using a recombinant ectonucleotide pyrophosphatase phosphodiesterase (NPP1)
WO2019060993A1 (en) 2017-09-28 2019-04-04 The Governors Of The University Of Alberta Retinoic acid-inducible gene i promoter and compositions and methods relating to same
GB201813528D0 (en) 2018-08-20 2018-10-03 Ucl Business Plc Factor IX encoding nucleotides
US10842885B2 (en) 2018-08-20 2020-11-24 Ucl Business Ltd Factor IX encoding nucleotides

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3636991A1 (de) 1986-03-03 1987-09-24 Transgene Gmbh Verfahren zur uebertragung organischer und/oder anorganischer substanzen auf ei- und/oder somazellen von tieren sowie entsprechende zusammensetzungen
GB8714426D0 (en) 1987-06-19 1987-07-22 Agricultural & Food Res Culture technique
US4997763A (en) 1987-07-31 1991-03-05 The United States Of America As Represented By The Secretary Of Agriculture Vectors for gene insertion into avian germ line
US5162215A (en) 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
GB8906214D0 (en) 1989-03-17 1989-05-04 Nat Res Dev Introducing an exogenous gene into birds
US5731178A (en) 1990-03-21 1998-03-24 Behringwerke Aktiengesellschaft Attachment-elements for stimulation of eukaryotic expression systems
US6027722A (en) 1990-10-25 2000-02-22 Nature Technology Corporation Vectors for gene transfer
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
US6825396B2 (en) 1996-06-12 2004-11-30 Board Of Trustees Operating Michigan State University Methods for tissue specific synthesis of protein in eggs of transgenic hens
AU750028B2 (en) 1997-08-22 2002-07-11 Regents Of The University Of California, The Production of proteins in eggs
JP2001520009A (ja) * 1997-10-16 2001-10-30 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド 鳥の導入遺伝子についてのマグナム特異的プロモーターを含むベクター
IL123411A0 (en) 1998-02-22 1998-09-24 Kimron Veterinary Inst High efficiency methods and compositions for integrating exogenous dna into genomic dna of sperm
EP1105469B1 (de) 1998-08-11 2010-09-29 University Of Hawaii Transgenese in säugetieren durch intracytoplasmatische injektion von spermien
JP2002543814A (ja) 1999-05-13 2002-12-24 セダーシナイ メディカル センター トランスジェニック種の発生と遺伝子療法用の雄性生殖細胞の遺伝的修飾法
AU777420B2 (en) * 1999-06-04 2004-10-14 Tranxenogen, Inc. Methods for manipulating the avian genome
US6423488B1 (en) 2000-01-15 2002-07-23 Avigenics, Inc High throughput screening assay for detecting a DNA sequence
EP1129617A1 (de) * 2000-02-15 2001-09-05 Ovo Biosciences, Inc. Methode um ein Vogelei in der Schale zu fertilisieren
US7049480B1 (en) * 2000-09-01 2006-05-23 Avigenics, Inc. Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy
US20020108132A1 (en) 2001-02-02 2002-08-08 Avigenics Inc. Production of a monoclonal antibody by a transgenic chicken
US20020116732A1 (en) 2001-02-13 2002-08-22 Leandro Christmann Microinjection assembly and methods for microinjecting and reimplanting avian eggs
US7176300B2 (en) 2001-03-30 2007-02-13 Avigenics, Inc. Avian lysozyme promoter
WO2002079447A2 (en) 2001-03-30 2002-10-10 Avigenics, Inc. Avian lysozyme promoter
US6875588B2 (en) 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use

Also Published As

Publication number Publication date
WO2003025146A2 (en) 2003-03-27
AU2002341746A1 (en) 2003-04-01
EP1438401B1 (de) 2011-06-15
EP1438401A4 (de) 2005-03-09
US20030126629A1 (en) 2003-07-03
EP1438401A2 (de) 2004-07-21
WO2003025146A3 (en) 2003-12-18
US7312374B2 (en) 2007-12-25

Similar Documents

Publication Publication Date Title
ATE513038T1 (de) Produktion eines transgenen vogels mittels cytoplasmainjektion
DE69842019D1 (de) Transgene vögel und proteinproduktion
AU2003303830A8 (en) Production of multimeric proteins
Dimitrov et al. Germline gene editing in chickens by efficient CRISPR-mediated homologous recombination in primordial germ cells
DE60226335D1 (en) Aviäres expressionssystem für fremdproteine
ATE405154T1 (de) Verfahren zur erschaffung transgener nicht- menschlicher tiere
EP1592773A4 (de) In vogelartigen und deren eiern exprimierte exogene proteine
EP1451316A4 (de) Verfahren zur erzeugung von transgenetischen vögeln und fischen
RU2016123135A (ru) Отличные от человека животные, содержащие гуманизированный ген индуцирующего пролиферацию лиганда
US6281408B1 (en) Efficient method for production of compound transgenic animals
DE60327537D1 (de) Genregulation in transgenen tieren unter verwendung eines vektors auf transposonbasis
NZ598769A (en) Transgenic chickens
JP2004129649A (ja) 新規なトランスジェニックゼブラフィッシュ、遺伝子断片、及びトランスジェニックゼブラフィッシュの生産方法
RU2017132331A (ru) Etv2 и его применения
McCoy et al. Creation and preliminary characterization of a Tp53 knockout rat
CN105039402A (zh) 一种改良猪肌肉品质的方法
US10246679B2 (en) Method for producing pluripotent stem cell having improved chimera forming ability from primed pluripotent stem cell, and composition and combination therefor
MX2024016133A (es) Elevada expresion de hemoproteina animal en plantas
US20210345592A1 (en) Genetically modified sterile avians and method for the reconstitution thereof
RU2018103234A (ru) Гуманизированная мышца сердца
EP1672076A4 (de) Verfahren zur konstruktion eines transgenen vogels unter verwendung eines lentivirusvektors und damit erhaltener transgener vogel
JP6366944B2 (ja) プライム型多能性幹細胞からキメラ形成能を向上させた多能性幹細胞を製造する方法並びにそのための組成物および組合せ
ATE540580T1 (de) Verfahren zur herstellung eines transgenen vogels.
ATE455176T1 (de) Transgene expressionskassetten zur expression von nukleinsäuren in nicht-reproduktiven blütengeweben von pflanzen
Okon et al. Transgenesis techniques and its application in poultry production

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties