ATE513038T1 - Produktion eines transgenen vogels mittels cytoplasmainjektion - Google Patents

Produktion eines transgenen vogels mittels cytoplasmainjektion

Info

Publication number
ATE513038T1
ATE513038T1 AT02775895T AT02775895T ATE513038T1 AT E513038 T1 ATE513038 T1 AT E513038T1 AT 02775895 T AT02775895 T AT 02775895T AT 02775895 T AT02775895 T AT 02775895T AT E513038 T1 ATE513038 T1 AT E513038T1
Authority
AT
Austria
Prior art keywords
cytoplasm
heterologous protein
bird
transgenic
methods
Prior art date
Application number
AT02775895T
Other languages
English (en)
Inventor
Jeffrey Rapp
Leandro Christmann
Original Assignee
Synageva Biopharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synageva Biopharma Corp filed Critical Synageva Biopharma Corp
Application granted granted Critical
Publication of ATE513038T1 publication Critical patent/ATE513038T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/23—Immunoglobulins specific features characterized by taxonomic origin from birds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/90—Vector systems having a special element relevant for transcription from vertebrates avian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Husbandry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
AT02775895T 2001-09-18 2002-09-18 Produktion eines transgenen vogels mittels cytoplasmainjektion ATE513038T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32296901P 2001-09-18 2001-09-18
US35155002P 2002-01-25 2002-01-25
PCT/US2002/029878 WO2003025146A2 (en) 2001-09-18 2002-09-18 Production of a transgenic avian by cytoplasmic injection

Publications (1)

Publication Number Publication Date
ATE513038T1 true ATE513038T1 (de) 2011-07-15

Family

ID=26983697

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02775895T ATE513038T1 (de) 2001-09-18 2002-09-18 Produktion eines transgenen vogels mittels cytoplasmainjektion

Country Status (5)

Country Link
US (1) US7312374B2 (de)
EP (1) EP1438401B1 (de)
AT (1) ATE513038T1 (de)
AU (1) AU2002341746A1 (de)
WO (1) WO2003025146A2 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040019923A1 (en) * 1997-10-16 2004-01-29 Ivarie Robert D. Exogenous proteins expressed in avians and their eggs
ATE489461T1 (de) * 1997-10-16 2010-12-15 Univ Georgia Transgene vögel und proteinproduktion
US20080124787A1 (en) * 2001-02-13 2008-05-29 Avigenics, Inc. Microinjection devices and methods of use
US20020116732A1 (en) * 2001-02-13 2002-08-22 Leandro Christmann Microinjection assembly and methods for microinjecting and reimplanting avian eggs
US7339090B2 (en) * 2001-02-13 2008-03-04 Avigenics, Inc. Microinjection devices and methods of use
US7541512B2 (en) * 2001-03-30 2009-06-02 Synageva Biopharma Corp. Avians containing a lysozyme promoter transgene
US20100333219A1 (en) * 2001-11-30 2010-12-30 Synageva Biopharma Corp. Methods of protein production using ovomucoid regulatory regions
US7294507B2 (en) * 2001-11-30 2007-11-13 Avigenics, Inc. Ovomucoid promoters and methods of use
US7335761B2 (en) * 2001-11-30 2008-02-26 Avigenics, Inc. Avian gene expression controlling regions
US7323618B2 (en) * 2002-02-01 2008-01-29 Origen Therapeutics, Inc. Tissue specific expression of exogenous proteins in transgenic chickens
US20060191026A1 (en) 2005-02-18 2006-08-24 Origen Therapeutics, Inc. Tissue specific expression of antibodies in chickens
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
EP1548114B8 (de) * 2002-08-13 2012-10-31 Kaneka Corporation Verfahren zur genexpression in transgenen vögeln unter verwendung eines retrovirusvektors und so erhaltene transgene vögel
US7803362B2 (en) 2003-01-24 2010-09-28 Synageva Biopharma Corp. Glycosylated interferon alpha
US20040255345A1 (en) * 2003-03-07 2004-12-16 Rapp Jeffrey C. Production of transgenic avians
WO2004080162A2 (en) * 2003-03-07 2004-09-23 Avigenics, Inc. Integrase mediated avian transgenesis
US20050273873A1 (en) * 2003-03-07 2005-12-08 Avigenics, Inc. Genomic modification
US20050198700A1 (en) * 2003-03-07 2005-09-08 Avigenics, Inc. Genomic modification
US20050034186A1 (en) * 2003-03-07 2005-02-10 Harvey Alex J. Site specific nucleic acid integration
JP2006523438A (ja) * 2003-04-15 2006-10-19 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド ニワトリ卵白アルブミン遺伝子領域を用いたトリ遺伝子組換え
US7381712B2 (en) * 2003-05-09 2008-06-03 Avigenics, Inc. In vivo transfection in avians
US20080222744A1 (en) * 2003-05-09 2008-09-11 Avigenics, Inc. In vivo transfection in avians
KR100569163B1 (ko) * 2003-08-11 2006-04-07 (주)아비코아생명공학연구소 정원세포를 이용한 조류 카이메라의 생산방법 및 조류 카이메라
US20050090001A1 (en) 2003-10-07 2005-04-28 Parker Stephen H. Cell lines and methods for producing proteins
US8071364B2 (en) * 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
EP1708564A4 (de) * 2004-01-15 2007-03-07 Avigenics Inc Produktion eines transgenen vogels mittels cytoplasmainjektion
US20060174364A1 (en) * 2004-03-01 2006-08-03 Avigenics, Inc. Artificial chromosomes and transchromosomic avians
US20060123504A1 (en) * 2004-12-07 2006-06-08 Avigenics, Inc. Methods of producing polyclonal antibodies
WO2006042214A1 (en) * 2004-10-08 2006-04-20 Avigenics, Inc. Modified integrase and methods of use
US7812127B2 (en) 2006-03-17 2010-10-12 Synageva Biopharma Corp. Glycosylated human G-CSF
EP2076530A4 (de) * 2006-11-09 2010-04-07 Synageva Biopharma Corp Aus vögeln gewonnenes erythropoietin
US20090105462A1 (en) * 2006-11-09 2009-04-23 Ivarie Robert D Glycosylated erythropoietin
WO2010036978A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
EP2342224A2 (de) * 2008-09-25 2011-07-13 TransGenRx, Inc. Neue vektoren zur herstellung von interferon
WO2010036976A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
WO2010118360A1 (en) 2009-04-09 2010-10-14 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Production of proteins using transposon-based vectors
US8815242B2 (en) * 2009-05-27 2014-08-26 Synageva Biopharma Corp. Avian derived antibodies
KR101808751B1 (ko) 2009-11-13 2017-12-13 그리폴스 테라퓨틱스 인코포레이티드 폰 빌레브란트 인자(ｖＷＦ)-함유 제제, 및 그와 관련된 방법, 키트 및 용도
GB201420139D0 (en) 2014-11-12 2014-12-24 Ucl Business Plc Factor IX gene therapy
WO2017111144A1 (ja) * 2015-12-25 2017-06-29 国立研究開発法人産業技術総合研究所 遺伝子改変家禽卵
CA3075956A1 (en) * 2017-09-19 2019-03-28 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Genome-edited birds
JP7511469B2 (ja) 2017-09-27 2024-07-05 イノザイム ファーマ， インコーポレイテッド 組換えエクトヌクレオチドピロホスファターゼ／ホスホジエステラーゼ（ｎｐｐ１）を使用して心血管機能を改善し、心血管疾患を処置するための方法。
US11519005B2 (en) 2017-09-28 2022-12-06 The Governors Of The University Of Alberta Retinoic acid-inducible gene I promoter and compositions and methods relating to same
GB201813528D0 (en) 2018-08-20 2018-10-03 Ucl Business Plc Factor IX encoding nucleotides
US10842885B2 (en) 2018-08-20 2020-11-24 Ucl Business Ltd Factor IX encoding nucleotides

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3636991A1 (de) 1986-03-03 1987-09-24 Transgene Gmbh Verfahren zur uebertragung organischer und/oder anorganischer substanzen auf ei- und/oder somazellen von tieren sowie entsprechende zusammensetzungen
GB8714426D0 (en) 1987-06-19 1987-07-22 Agricultural & Food Res Culture technique
US4997763A (en) 1987-07-31 1991-03-05 The United States Of America As Represented By The Secretary Of Agriculture Vectors for gene insertion into avian germ line
US5162215A (en) 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
GB8906214D0 (en) 1989-03-17 1989-05-04 Nat Res Dev Introducing an exogenous gene into birds
US5731178A (en) 1990-03-21 1998-03-24 Behringwerke Aktiengesellschaft Attachment-elements for stimulation of eukaryotic expression systems
US6027722A (en) 1990-10-25 2000-02-22 Nature Technology Corporation Vectors for gene transfer
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
US6825396B2 (en) * 1996-06-12 2004-11-30 Board Of Trustees Operating Michigan State University Methods for tissue specific synthesis of protein in eggs of transgenic hens
CA2311116A1 (en) 1997-08-22 1999-03-04 University Of Guelph Production of proteins in eggs
ATE489461T1 (de) * 1997-10-16 2010-12-15 Univ Georgia Transgene vögel und proteinproduktion
IL123411A0 (en) 1998-02-22 1998-09-24 Kimron Veterinary Inst High efficiency methods and compositions for integrating exogenous dna into genomic dna of sperm
RU2267270C2 (ru) 1998-08-11 2006-01-10 Юнивесити Оф Гаваи Трансгенез у млекопитающих путем интрацитоплазматической инъекции спермы
JP2002543814A (ja) 1999-05-13 2002-12-24 セダーシナイ メディカル センター トランスジェニック種の発生と遺伝子療法用の雄性生殖細胞の遺伝的修飾法
WO2000075300A2 (en) * 1999-06-04 2000-12-14 Tranxenogen, Inc. Methods for manipulating the avian genome
US6423488B1 (en) 2000-01-15 2002-07-23 Avigenics, Inc High throughput screening assay for detecting a DNA sequence
TWI314163B (en) * 2000-02-15 2009-09-01 Ovo Biosciences Inc In ovo activation of an avian egg in the shell
US7049480B1 (en) * 2000-09-01 2006-05-23 Avigenics, Inc. Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy
US20020108132A1 (en) 2001-02-02 2002-08-08 Avigenics Inc. Production of a monoclonal antibody by a transgenic chicken
US20020116732A1 (en) 2001-02-13 2002-08-22 Leandro Christmann Microinjection assembly and methods for microinjecting and reimplanting avian eggs
US7176300B2 (en) 2001-03-30 2007-02-13 Avigenics, Inc. Avian lysozyme promoter
AU2002255995A1 (en) 2001-03-30 2002-10-15 Avigenics, Inc. Avian lysozyme promoter
US6875588B2 (en) 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use

Also Published As

Publication number Publication date
EP1438401B1 (de) 2011-06-15
US20030126629A1 (en) 2003-07-03
WO2003025146A2 (en) 2003-03-27
AU2002341746A1 (en) 2003-04-01
EP1438401A4 (de) 2005-03-09
WO2003025146A3 (en) 2003-12-18
EP1438401A2 (de) 2004-07-21
US7312374B2 (en) 2007-12-25

Similar Documents

Publication Publication Date Title
EP1438401A4 (de) Produktion eines transgenen vogels mittels cytoplasmainjektion
ATE489461T1 (de) Transgene vögel und proteinproduktion
AU2003303830A8 (en) Production of multimeric proteins
Dimitrov et al. Germline gene editing in chickens by efficient CRISPR-mediated homologous recombination in primordial germ cells
DE60226335D1 (en) Aviäres expressionssystem für fremdproteine
ATE405154T1 (de) Verfahren zur erschaffung transgener nicht- menschlicher tiere
NO20076087L (no) Serum-fri stabil transfeksjon og fremstilling av rekombinante humane proteiner i humane cellelinjer
WO2003064627A3 (en) Chimeric bird from embryonic stem cells
WO2004067713A3 (en) Exogenous proteins expressed in avians and their eggs
RU2016123135A (ru) Отличные от человека животные, содержащие гуманизированный ген индуцирующего пролиферацию лиганда
WO2004015123A8 (en) Tissue specific expression of exogenous proteins in transgenic chickens
US6281408B1 (en) Efficient method for production of compound transgenic animals
DE60327537D1 (de) Genregulation in transgenen tieren unter verwendung eines vektors auf transposonbasis
NZ598769A (en) Transgenic chickens
WO2006084035A3 (en) Transgenic chickens
JP2004129649A (ja) 新規なトランスジェニックゼブラフィッシュ、遺伝子断片、及びトランスジェニックゼブラフィッシュの生産方法
RU2017132331A (ru) Etv2 и его применения
McCoy et al. Creation and preliminary characterization of a Tp53 knockout rat
CN105039402A (zh) 一种改良猪肌肉品质的方法
US10246679B2 (en) Method for producing pluripotent stem cell having improved chimera forming ability from primed pluripotent stem cell, and composition and combination therefor
MX2024016133A (es) Elevada expresion de hemoproteina animal en plantas
RU2017142635A (ru) Способы доставки агента в икринки и эмбрионы мечущих икру водных животных для лекарственного скрининга, анализ токсичности агента и получение бесплодной рыбы
CA3115886A1 (en) Genetically modified sterile avians and method for the reconstitution thereof
EP1672076A4 (de) Verfahren zur konstruktion eines transgenen vogels unter verwendung eines lentivirusvektors und damit erhaltener transgener vogel
JP6366944B2 (ja) プライム型多能性幹細胞からキメラ形成能を向上させた多能性幹細胞を製造する方法並びにそのための組成物および組合せ

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties