ATE517192T1 - Verfahren zur isolierung von zellen - Google Patents

Verfahren zur isolierung von zellen

Info

Publication number
ATE517192T1
ATE517192T1 AT07784617T AT07784617T ATE517192T1 AT E517192 T1 ATE517192 T1 AT E517192T1 AT 07784617 T AT07784617 T AT 07784617T AT 07784617 T AT07784617 T AT 07784617T AT E517192 T1 ATE517192 T1 AT E517192T1
Authority
AT
Austria
Prior art keywords
cells
isolation
isolating
sample
complex sample
Prior art date
Application number
AT07784617T
Other languages
English (en)
Inventor
Peter Rossmanith
Ingeborg Hein
Martin Wagner
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Application granted granted Critical
Publication of ATE517192T1 publication Critical patent/ATE517192T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT07784617T 2006-08-10 2007-08-10 Verfahren zur isolierung von zellen ATE517192T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT13462006 2006-08-10
PCT/AT2007/000388 WO2008017097A1 (en) 2006-08-10 2007-08-10 Method for isolating cells

Publications (1)

Publication Number Publication Date
ATE517192T1 true ATE517192T1 (de) 2011-08-15

Family

ID=38645811

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07784617T ATE517192T1 (de) 2006-08-10 2007-08-10 Verfahren zur isolierung von zellen

Country Status (4)

Country Link
US (1) US9328326B2 (de)
EP (1) EP2049677B1 (de)
AT (1) ATE517192T1 (de)
WO (1) WO2008017097A1 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2443226T3 (pl) * 2009-06-18 2015-04-30 Merck Patent Gmbh Sposób izolowania komórek
EP2266696A1 (de) 2009-06-23 2010-12-29 Veterinärmedizinische Universität Wien Vorrichtung zur Vereinzelung von Mikroobjekten
ES2565335T3 (es) 2009-08-17 2016-04-04 Merck Patent Gmbh Método para aislar virus
EP2325312A1 (de) 2009-11-19 2011-05-25 Qiagen GmbH Verfahren zur selektiven Anreicherung und Isolierung mikrobieller und optional zusätzlich viraler Nukleinsäuren
US8808683B2 (en) 2010-06-02 2014-08-19 Merck Patent Gmbh Genetically modified bacterium of the species Listeria monocytogenes
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
ES2656947T3 (es) 2011-04-27 2018-03-01 Merck Patent Gmbh Método para lisar células
CN103813845B (zh) * 2011-07-22 2016-08-17 生物梅里埃有限公司 从培养物离析微生物的方法和试剂盒
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
EP2684947A1 (de) * 2012-07-09 2014-01-15 Biocartis SA Verfahren zum Filtern einer biologischen Probe
EP2875156A4 (de) 2012-07-19 2016-02-24 Ariosa Diagnostics Inc Sequentielle ligationsbasierte multiplex-detektion von genetischen varianten
KR101933621B1 (ko) 2012-09-28 2018-12-28 삼성전자주식회사 소포를 분리하기 위한 조성물, 키트 및 이를 이용하여 소포를 분리하는 방법
ES2787174T3 (es) 2013-12-18 2020-10-15 Asahi Chemical Ind Procedimiento para detectar bacterias coliformes contenidas en la leche
US8906972B1 (en) 2013-12-27 2014-12-09 King Fahd University Of Petroleum And Minerals Aqueous two phase (ATPSs) of pH-responsive poly(phosphonate-alt-sulfur dioxide) with PEG
KR102535489B1 (ko) 2014-06-13 2023-05-22 큐-리네아 에이비 미생물 검출 방법 및 특성 규명 방법
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
GB201511129D0 (en) 2015-06-24 2015-08-05 Linea Ab Q Method of determining antimicrobial susceptibility of a microorganism
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
RU2659197C2 (ru) * 2016-07-18 2018-06-28 ФГБУ "Новосибирский научно-исследовательский институт туберкулеза" Министерства здравоохранения Российской Федерации Способ выделения ДНК клинических изолятов Mycobacterium tuberculosis из ткани легкого
GB201617713D0 (en) 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
GB201716883D0 (en) 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
CN109439658A (zh) * 2018-12-27 2019-03-08 中国农业科学院北京畜牧兽医研究所 一种裂解液及其在液态奶中提取核酸的方法
US20230183777A1 (en) * 2021-12-13 2023-06-15 BioMed Technology Holdings Limited Hypoosmotic stabilizer for genomic dna of gut microbiota, and preparation method and use thereof
CN114657125B (zh) * 2022-04-29 2023-08-08 中国科学院海洋研究所 鲨鱼单个核细胞的分离方法、鲨鱼稀释液及其用途
EP4389911A1 (de) * 2022-12-21 2024-06-26 Mobidiag Oy Verfahren und systeme zur isolierung eines analyten
FR3164230A1 (fr) 2024-07-03 2026-01-09 Commissariat A L' Energie Atomique Et Aux Energies Alternatives Procédé d’obtention d’un échantillon de matrice alimentaire fluidifié et utilisation de l’échantillon obtenu pour la détection de bactéries

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3745090A (en) * 1970-08-04 1973-07-10 Nasa Method of detecting and counting bacteria in body fluids
US5346999A (en) * 1985-01-18 1994-09-13 Applied Biosystems, Inc. Method of nucleic acid extraction
US4673733A (en) * 1985-04-11 1987-06-16 Sudhish Chandra Treatment of biological and pharmaceutical products adsorbed on a solid phase with virus and pyrogen inactivating agents
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5420017A (en) * 1991-01-24 1995-05-30 Orion Corporation Ltd. Method and kit for the detection of microorganisms
DE69228116T2 (de) * 1991-06-03 1999-05-20 Arch Development Corp., Chicago, Ill. Verfahren und zusammensetzungen des genetischen stressantwortsystems
US5459253A (en) * 1991-07-19 1995-10-17 Pharmacia P-L Biochemicals Inc. M-RNA purification
DE69223617T2 (de) * 1992-09-18 1998-04-09 Amersham Int Plc Verfahren zum Auffangen von Zellkernen und dafür verwendete Vorrichtung
FR2698879B1 (fr) * 1992-12-09 1995-01-13 Rhone Poulenc Biochimie gellules modifiées au niveau du catabolisme de la bétaïne, préparation et utilisations, notamment pour la production de métabolites ou d'enzymes.
US20040132082A1 (en) * 1995-02-14 2004-07-08 Bio101 Method for isolating DNA
DE19607202A1 (de) 1996-02-26 1997-08-28 Uwe Dr Michel Reagenzienkit zur Präparation von Nukleinsäuren
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
US20010007153A1 (en) * 1997-06-16 2001-07-05 Jennifer June Brown Solid chimeric organs, animal models having same, process for preparing same, non-tumorigenic immortalized human cell lines, susceptible cells and cytopathic mammalian viruses
DE60026641T2 (de) * 1999-07-15 2007-01-25 Qiagen Gmbh Verfahren zur Trennung von teilchenförmigen Substraten aus einer Lösung indem man den Teilchenverlust minimiert
EP1237928A1 (de) * 1999-12-13 2002-09-11 ZLB Bioplasma AG Verbesserung des therapeutischen potential von immunoglobulinpreparationen
GB0001450D0 (en) * 2000-01-21 2000-03-08 Genpoint As Cell isolation method
US7322369B2 (en) * 2000-04-11 2008-01-29 The United States Of America As Represented By The Secretary Of Agriculture Methods of detaching microorganisms from, or of inhibiting microbial attachment to, animal or poultry carcasses or seafood or parts thereof
WO2001088183A2 (en) 2000-05-18 2001-11-22 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services, Centers For Disease Control And Prevention Method for the isolation of helicobacter pylori
EP1311860A2 (de) 2000-08-21 2003-05-21 Queen's University At Kingston Verfahren und testkit zur auftrennung und zum nachweis von proteinen in biologischen proben
EP1978110B1 (de) * 2000-09-06 2010-05-26 Transnetyx, Inc. Computer-basiertes Verfahren und System zum Screenen von genomischer DNS
US20030101476A1 (en) * 2000-12-12 2003-05-29 Short Jay M. Recombinant phytases and uses thereof
GB0030669D0 (en) * 2000-12-15 2001-01-31 Unilever Plc Detergent compositions
MXPA03005624A (es) * 2000-12-22 2004-12-03 Vaurox Llc Metodos para clonar mamiferos usando cromatina reprogramada del donador o celulas donadoras.
WO2002065125A1 (en) * 2001-02-13 2002-08-22 Invitrogen Corporation Methods and compositions for isolation of biological macromolecules
JP3578144B2 (ja) * 2002-01-16 2004-10-20 住友電気工業株式会社 回折型光学部品の光学特性測定装置及び測定方法
JP4261366B2 (ja) 2002-03-26 2009-04-30 カウンスル オブ サイエンティフィック アンド インダストリアル リサーチ 食物を毒する細菌を検出するためのプライマー及びその使用
US20040248089A1 (en) * 2002-03-27 2004-12-09 Banada Padmanabha Padmapriya Primes for detecting food poisoning bacteria and a method thereof
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
WO2005010204A2 (en) * 2003-07-21 2005-02-03 Creighton University Rapid nucleic acid isolation method and compositions therefore
DE60330399D1 (de) 2003-07-29 2010-01-14 All India Inst Med Trichmikroskopie, kultur und polymerasekettenreaktion unter verwendung prozessierter klinischer proben und kit davon
CA2559943A1 (en) * 2004-03-15 2005-10-06 Purdue Research Foundation Cell concentration and pathogen recovery
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
DE102005040347A1 (de) * 2005-08-25 2007-03-01 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von Listerien
US20080131955A1 (en) * 2006-11-30 2008-06-05 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA

Also Published As

Publication number Publication date
EP2049677A1 (de) 2009-04-22
US9328326B2 (en) 2016-05-03
WO2008017097A1 (en) 2008-02-14
EP2049677B1 (de) 2011-07-20
US20100184210A1 (en) 2010-07-22

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