ATE524484T1 - In vitro verfahren zur sequenzspezifischen biotinylierung von polypeptiden - Google Patents

In vitro verfahren zur sequenzspezifischen biotinylierung von polypeptiden

Info

Publication number: ATE524484T1
Authority: AT; Austria
Prior art keywords: polypeptide; biotinylated; reaction mixture; enzymatically active; active domain
Prior art date: 2001-09-25

Application number

AT02021322T

Other languages

English (en)

Inventor

Dorothee Ambrosius

Martin Lanzendoerfer

Michael Schraeml

Manfred Watzele

Original Assignee

Hoffmann La Roche

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-09-25

Filing date

2002-09-20

Publication date

2011-09-15

2002-09-20 Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche

2011-09-15 Application granted granted Critical

2011-09-15 Publication of ATE524484T1 publication Critical patent/ATE524484T1/de

Links

229920001184 polypeptide Polymers 0.000 title abstract 8
108090000765 processed proteins & peptides Proteins 0.000 title abstract 8
102000004196 processed proteins & peptides Human genes 0.000 title abstract 8
238000000034 method Methods 0.000 title abstract 2
230000006287 biotinylation Effects 0.000 title 1
238000007413 biotinylation Methods 0.000 title 1
238000000338 in vitro Methods 0.000 title 1
102000004190 Enzymes Human genes 0.000 abstract 3
108090000790 Enzymes Proteins 0.000 abstract 3
239000011541 reaction mixture Substances 0.000 abstract 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 2
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 abstract 1
XKMLYUALXHKNFT-UUOKFMHZSA-N Guanosine-5'-triphosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XKMLYUALXHKNFT-UUOKFMHZSA-N 0.000 abstract 1
150000001413 amino acids Chemical class 0.000 abstract 1
229960002685 biotin Drugs 0.000 abstract 1
235000020958 biotin Nutrition 0.000 abstract 1
239000011616 biotin Substances 0.000 abstract 1
238000011065 in-situ storage Methods 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
239000002773 nucleotide Substances 0.000 abstract 1
125000003729 nucleotide group Chemical group 0.000 abstract 1
238000010647 peptide synthesis reaction Methods 0.000 abstract 1
210000003705 ribosome Anatomy 0.000 abstract 1
239000000758 substrate Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
- C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/13—Labelling of peptides

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Biochemistry (AREA)
Analytical Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

AT02021322T 2001-09-25 2002-09-20 In vitro verfahren zur sequenzspezifischen biotinylierung von polypeptiden ATE524484T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP01122554		2001-09-25
EP01129681		2001-12-13

Publications (1)

Publication Number	Publication Date
ATE524484T1 true ATE524484T1 (de)	2011-09-15

Family

ID=26076717

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT02021322T ATE524484T1 (de)	2001-09-25	2002-09-20	In vitro verfahren zur sequenzspezifischen biotinylierung von polypeptiden

Country Status (4)

Country	Link
US (2)	US20030092073A1 (de)
JP (1)	JP2003153698A (de)
AT (1)	ATE524484T1 (de)
ES (1)	ES2372029T3 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20040067532A1 (en)	2002-08-12	2004-04-08	Genetastix Corporation	High throughput generation and affinity maturation of humanized antibody
US8877688B2 (en)	2007-09-14	2014-11-04	Adimab, Llc	Rationally designed, synthetic antibody libraries and uses therefor
JP5933894B2 (ja)	2007-09-14	2016-06-15	アディマブ，エルエルシー	合理的に設計された、合成抗体ライブラリおよびその使用
US12529164B2 (en)	2007-09-14	2026-01-20	Adimab, Llc	Rationally designed, synthetic antibody libraries and uses therefor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5478730A (en) *	1988-12-21	1995-12-26	Institute Of Protein Research	Method of preparing polypeptides in cell-free translation system
US5571690A (en) *	1991-05-08	1996-11-05	The University Of Virginia Patents Foundation	Method for the cell-free synthesis of proteins
WO1995004069A1 (en) *	1993-07-30	1995-02-09	Affymax Technologies N.V.	Biotinylation of proteins

2002
- 2002-09-20 AT AT02021322T patent/ATE524484T1/de not_active IP Right Cessation
- 2002-09-20 ES ES02021322T patent/ES2372029T3/es not_active Expired - Lifetime
- 2002-09-20 US US10/251,313 patent/US20030092073A1/en not_active Abandoned
- 2002-09-25 JP JP2002278364A patent/JP2003153698A/ja active Pending
2007
- 2007-09-28 US US11/863,733 patent/US20080187957A1/en not_active Abandoned

Also Published As

Publication number	Publication date
ES2372029T3 (es)	2012-01-13
US20080187957A1 (en)	2008-08-07
US20030092073A1 (en)	2003-05-15
JP2003153698A (ja)	2003-05-27

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Legal Events

Date	Code	Title	Description
2012-03-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties